MAGED4B ORF clone FHC00934

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Halotag ORF Clone FHC00934

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Clone info.

Species

human

Clone Name

pFN21ASDA1859 Sequence (with vector) Restriction Map

Product ID

FHC00934

Flexi Clone PID

FXC00934

ORF Sequence

DNA sequence (2223 bp)
Protein sequence (741 aa)

Linker Type

Flexi_RBS type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB384309]

Gene

KIAA1859

Symbol

MAGED4B

Description

melanoma antigen family D4B, transcript variant 1

Original Clone ID

bm01165

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 2223 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 741 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS14338.1 3.4e-174 100.0 MAGED4B CCDS35281.1 1.4e-173 100.0 MAGED4B CCDS48116.1 1.4e-173 100.0 MAGED4 CCDS65261.1 8.7e-128 97.9 MAGED4 CCDS56602.1 8.7e-128 97.9 MAGED4B The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR002190 420 589 PF01454 MAGE protein ProfileScan IPR002190 413 611 PS50838 MAGE protein
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_110428 741 300765 100.0 100.0 XP_006724670 741 300765 100.0 100.0 NP_001258992 741 300702 100.0 100.0 XP_006724671 741 300765 100.0 100.0 NP_803879 741 300765 100.0 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21ASDA1859 Download

>KIAA1859  2223 bp
ATGGCTGAGGGAAGCTTCAGCGTGCAATCGGAAAGCTACAGTGTTGAAGACATGGATGAG
GGTAGCGACGAAGTCGGGGAGGAAGAGATGGTTGAAGGCAACGACTATGAAGAATTCGGT
GCGTTTGGTGGCTATGGCACCCTCACCAGCTTTGACATCCATATCCTCAGAGCCTTCGGA
AGCTTGGGTCCAGGCCTTCGCATCTTATCGAATGAGCCCTGGGAACTGGAAAACCCTGTG
CTGGCCCAGACCCTGGTGGAGGCATTGCAGCTGGATCCGGAAACACTTGCCAATGAGACG
GCCGCCCGTGCTGCCAACGTAGCCCGCGCCGCCGCCTCCAACCGTGCGGCTCGGGCCGCT
GCCGCCGCTGCCCGTACCGCCTTCAGTCAGGTGGTCGCTAGCCACCGGGTGGCCACGCCG
CAGGTCTCAGGAGAGGATACCCAGCCCACGACCTACGCCGCCGAGGCTCAGGGGCCCACC
CCTGAGCCACCCCTTGCTTCTCCGCAGACCTCCCAGATGTTAGTCACCAGTAAGATGGCT
GCCCCCGAGGCTCCGGCAACCTCCGCACAGTCCCAGACAGGCTCCCCGGCCCAGGAGGCT
GCTACTGAGGGCCCTAGTAGCGCCTGTGCTTTCTCTCAGGCTCCGTGTGCCAGGGAGGTG
GACGCCAACCGGCCCAGCACAGCCTTCCTGGGCCAGAATGATGTCTTCGATTTCACTCAG
CCGGCAGGTGTCAGTGGCATGGCCTTCCCGCGCCCCAAGAGACCTGCCCCAGCCCAAGAG
GCTGCCACAGAGGGCCCCAGTGCTGCCTCTGGTGTGCCCCAGACGGGACCTGGCAGGGAG
GTGGCAGCCACCCGGCCCAAGACCACCAAGTCGGGGAAGGCGCTGGCCAAGACTCGGTGG
GTGGAGCCTCAGAATGTTGTGGCAGCAGCTGCTGCCAAGGCCAAGATGGCCACGAGCATC
CCTGAGCCGGAGGGTGCAGCTGCTGCCACTGCTCAGCACAGTGCTGAGCCCTGGGCCAGG
ATGGGAGGCAAGAGGACCAAGAAGTCCAAGCACCTGGATGATGAGTATGAGAGCAGCGAG
GAGGAGAGAGAGACTCCCGCGGTCCCACCCACCTGGAGAGCATCACAGCCCTCATTGACG
GTGCGGGCTCAGTTGGCCCCTCGGCCCCCGATGGCCCCGAGGTCCCAGATACCCTCAAGG
CACGTACTGTGCCTGCCCCCCCGCAACGTGACCCTTCTGCAGGAGAGGGCAAATAAGTTG
GTGAAATACCTGATGATTAAGGACTACAAGAAGATCCCCATCAAGCGCGCAGACATGCTG
AAGGATGTCATCAGAGAATATGATGAACATTTCCCTGAGATCATTGAACGAGCAACGTAC
ACCCTGGAAAAGAAGTTTGGGATCCACCTGAAGGAGATCGACAAGGAAGAACACCTGTAT
ATTCTTGTCTGCACACGGGACTCCTCAGCTCGCCTCCTTGGAAAAACCAAGGACACTCCC
AGGCTGAGTCTCCTCTTGGTGATTCTGGGCGTCATCTTCATGAATGGCAACCGTGCCAGC
GAGGCTGTCCTCTGGGAGGCACTACGCAAGATGGGACTGCGCCCTGGGGTGAGGCACCCA
TTCCTCGGCGATCTGAGGAAGCTCATCACAGATGACTTTGTGAAGCAGAAGTACCTGGAA
TACAAGAAGATCCCCAACAGCAACCCACCTGAGTATGAATTCCTCTGGGGCCTGCGAGCC
CGCCATGAGACCAGCAAGATGAGGGTCCTGAGATTCATCGCCCAGAATCAGAACCGAGAC
CCCCGGGAATGGAAGGCTCATTTCTTGGAGGCTGTGGATGATGCTTTCAAGACAATGGAT
GTGGATATGGCCGAGGAACATGCCAGGGCCCAGATGAGGGCCCAGATGAATATCGGGGAT
GAAGCGCTGATTGGACGGTGGAGCTGGGATGACATACAAGTCGAGCTCCTGACCTGGGAT
GAGGACGGAGATTTTGGCGATGCCTGGGCCAGGATCCCCTTTGCTTTCTGGGCCAGATAC
CATCAGTACATTCTGAATAGCAACCGTGCCAACAGGAGGGCCACGTGGAGAGCTGGCGTC
AGCAGTGGCACCAATGGAGGGGCCAGCACCAGCGTCCTAGATGGCCCCAGCACCAGCTCC
ACCATCCGGACCAGAAATGCTGCCAGAGCTGGCGCCAGCTTCTTCTCCTGGATCCAGCAC
CGT

ORF Protein

Cloned ORF protein sequence for pFN21ASDA1859 Download

>KIAA1859  741 aa
MAEGSFSVQSESYSVEDMDEGSDEVGEEEMVEGNDYEEFGAFGGYGTLTSFDIHILRAFG
SLGPGLRILSNEPWELENPVLAQTLVEALQLDPETLANETAARAANVARAAASNRAARAA
AAAARTAFSQVVASHRVATPQVSGEDTQPTTYAAEAQGPTPEPPLASPQTSQMLVTSKMA
APEAPATSAQSQTGSPAQEAATEGPSSACAFSQAPCAREVDANRPSTAFLGQNDVFDFTQ
PAGVSGMAFPRPKRPAPAQEAATEGPSAASGVPQTGPGREVAATRPKTTKSGKALAKTRW
VEPQNVVAAAAAKAKMATSIPEPEGAAAATAQHSAEPWARMGGKRTKKSKHLDDEYESSE
EERETPAVPPTWRASQPSLTVRAQLAPRPPMAPRSQIPSRHVLCLPPRNVTLLQERANKL
VKYLMIKDYKKIPIKRADMLKDVIREYDEHFPEIIERATYTLEKKFGIHLKEIDKEEHLY
ILVCTRDSSARLLGKTKDTPRLSLLLVILGVIFMNGNRASEAVLWEALRKMGLRPGVRHP
FLGDLRKLITDDFVKQKYLEYKKIPNSNPPEYEFLWGLRARHETSKMRVLRFIAQNQNRD
PREWKAHFLEAVDDAFKTMDVDMAEEHARAQMRAQMNIGDEALIGRWSWDDIQVELLTWD
EDGDFGDAWARIPFAFWARYHQYILNSNRANRRATWRAGVSSGTNGGASTSVLDGPSTSS
TIRTRNAARAGASFFSWIQHR

ORF+Vector

Nucleotide Sequence (with vector) for pFN21ASDA1859 Download

>pFN21ASDA1859  6952 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCTTTCGAAGGAGATAGAACCATGGCTGAGGGAAGCTT
CAGCGTGCAATCGGAAAGCTACAGTGTTGAAGACATGGATGAGGGTAGCGACGAAGTCGG
GGAGGAAGAGATGGTTGAAGGCAACGACTATGAAGAATTCGGTGCGTTTGGTGGCTATGG
CACCCTCACCAGCTTTGACATCCATATCCTCAGAGCCTTCGGAAGCTTGGGTCCAGGCCT
TCGCATCTTATCGAATGAGCCCTGGGAACTGGAAAACCCTGTGCTGGCCCAGACCCTGGT
GGAGGCATTGCAGCTGGATCCGGAAACACTTGCCAATGAGACGGCCGCCCGTGCTGCCAA
CGTAGCCCGCGCCGCCGCCTCCAACCGTGCGGCTCGGGCCGCTGCCGCCGCTGCCCGTAC
CGCCTTCAGTCAGGTGGTCGCTAGCCACCGGGTGGCCACGCCGCAGGTCTCAGGAGAGGA
TACCCAGCCCACGACCTACGCCGCCGAGGCTCAGGGGCCCACCCCTGAGCCACCCCTTGC
TTCTCCGCAGACCTCCCAGATGTTAGTCACCAGTAAGATGGCTGCCCCCGAGGCTCCGGC
AACCTCCGCACAGTCCCAGACAGGCTCCCCGGCCCAGGAGGCTGCTACTGAGGGCCCTAG
TAGCGCCTGTGCTTTCTCTCAGGCTCCGTGTGCCAGGGAGGTGGACGCCAACCGGCCCAG
CACAGCCTTCCTGGGCCAGAATGATGTCTTCGATTTCACTCAGCCGGCAGGTGTCAGTGG
CATGGCCTTCCCGCGCCCCAAGAGACCTGCCCCAGCCCAAGAGGCTGCCACAGAGGGCCC
CAGTGCTGCCTCTGGTGTGCCCCAGACGGGACCTGGCAGGGAGGTGGCAGCCACCCGGCC
CAAGACCACCAAGTCGGGGAAGGCGCTGGCCAAGACTCGGTGGGTGGAGCCTCAGAATGT
TGTGGCAGCAGCTGCTGCCAAGGCCAAGATGGCCACGAGCATCCCTGAGCCGGAGGGTGC
AGCTGCTGCCACTGCTCAGCACAGTGCTGAGCCCTGGGCCAGGATGGGAGGCAAGAGGAC
CAAGAAGTCCAAGCACCTGGATGATGAGTATGAGAGCAGCGAGGAGGAGAGAGAGACTCC
CGCGGTCCCACCCACCTGGAGAGCATCACAGCCCTCATTGACGGTGCGGGCTCAGTTGGC
CCCTCGGCCCCCGATGGCCCCGAGGTCCCAGATACCCTCAAGGCACGTACTGTGCCTGCC
CCCCCGCAACGTGACCCTTCTGCAGGAGAGGGCAAATAAGTTGGTGAAATACCTGATGAT
TAAGGACTACAAGAAGATCCCCATCAAGCGCGCAGACATGCTGAAGGATGTCATCAGAGA
ATATGATGAACATTTCCCTGAGATCATTGAACGAGCAACGTACACCCTGGAAAAGAAGTT
TGGGATCCACCTGAAGGAGATCGACAAGGAAGAACACCTGTATATTCTTGTCTGCACACG
GGACTCCTCAGCTCGCCTCCTTGGAAAAACCAAGGACACTCCCAGGCTGAGTCTCCTCTT
GGTGATTCTGGGCGTCATCTTCATGAATGGCAACCGTGCCAGCGAGGCTGTCCTCTGGGA
GGCACTACGCAAGATGGGACTGCGCCCTGGGGTGAGGCACCCATTCCTCGGCGATCTGAG
GAAGCTCATCACAGATGACTTTGTGAAGCAGAAGTACCTGGAATACAAGAAGATCCCCAA
CAGCAACCCACCTGAGTATGAATTCCTCTGGGGCCTGCGAGCCCGCCATGAGACCAGCAA
GATGAGGGTCCTGAGATTCATCGCCCAGAATCAGAACCGAGACCCCCGGGAATGGAAGGC
TCATTTCTTGGAGGCTGTGGATGATGCTTTCAAGACAATGGATGTGGATATGGCCGAGGA
ACATGCCAGGGCCCAGATGAGGGCCCAGATGAATATCGGGGATGAAGCGCTGATTGGACG
GTGGAGCTGGGATGACATACAAGTCGAGCTCCTGACCTGGGATGAGGACGGAGATTTTGG
CGATGCCTGGGCCAGGATCCCCTTTGCTTTCTGGGCCAGATACCATCAGTACATTCTGAA
TAGCAACCGTGCCAACAGGAGGGCCACGTGGAGAGCTGGCGTCAGCAGTGGCACCAATGG
AGGGGCCAGCACCAGCGTCCTAGATGGCCCCAGCACCAGCTCCACCATCCGGACCAGAAA
TGCTGCCAGAGCTGGCGCCAGCTTCTTCTCCTGGATCCAGCACCGTTACGTAGTTTAAAC
GAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATC
CGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAAC
TAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTT
GGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCT
ATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATT
CATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTC
TACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTC
CAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTC
TACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCT
ATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGA
TAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCC
CTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTG
AAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTC
AACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACT
TTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTC
GGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAG
CATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGAT
AACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTT
TTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAA
GCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGC
AAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATG
GAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATT
GCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA
GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGAT
GAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAA
ATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTAT
CCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCA
GGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGC
ATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACC
AGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCG
GATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTA
GGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCG
TTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGAC
ACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAG
GCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTAT
TTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGAT
CCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGC
GCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGT
GGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCT
AGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGG
TGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCT
CTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCG
GGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCG
GTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAG
ATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT