(Circular) (Six-base) MAPSORT of: pF1KE2752.seq Check: 4268 from: 1 to: 6599 pF1KE2752 6599 bp With 182 enzymes: SgfI * August 1, 2019 16:47 .. AccI GT'mk_AC Cuts at: 1352 3637 1352 Size: 2285 4314 Acc65I G'GTAC_C Cuts at: 654 1603 3615 654 Size: 949 2012 3638 Fragments arranged by size: 3638 2012 949 AclI AA'CG_TT Cuts at: 6439 6439 Size: 6599 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 271 1825 2467 2672 2926 2953 3214 4424 4856 Size: 1554 642 205 254 27 261 1210 432 Cuts at: 4856 5603 5871 271 Size: 747 268 999 Fragments arranged by size: 1554 1210 999 747 642 432 268 261 254 205 27 AflII C'TTAA_G Cuts at: 151 151 Size: 6599 AflIII A'CryG_T Cuts at: 197 212 3951 5055 6032 197 Size: 15 3739 1104 977 764 Fragments arranged by size: 3739 1104 977 764 15 AgeI A'CCGG_T Cuts at: 4987 4987 Size: 6599 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 231 241 231 Size: 10 6589 AlwNI CAG_nnn'CTG Cuts at: 730 1455 2868 3538 3904 5471 730 Size: 725 1413 670 366 1567 1858 Fragments arranged by size: 1858 1567 1413 725 670 366 ApaLI G'TGCA_C Cuts at: 5369 5369 Size: 6599 ApoI r'AATT_y Cuts at: 1883 2623 3123 3410 3603 5952 6208 1883 Size: 740 500 287 193 2349 256 2274 Fragments arranged by size: 2349 2274 740 500 287 256 193 AseI AT'TA_AT Cuts at: 20 20 Size: 6599 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 6599 AvaI C'yCGr_G Cuts at: 2342 3619 2342 Size: 1277 5322 BamHI G'GATC_C Cuts at: 393 1660 2919 3624 393 Size: 1267 1259 705 3368 Fragments arranged by size: 3368 1267 1259 705 BanI G'GyrC_C Cuts at: 654 1575 1603 2269 3034 3615 4029 4277 4312 Size: 921 28 666 765 581 414 248 35 Cuts at: 4312 654 Size: 2941 Fragments arranged by size: 2941 921 765 666 581 414 248 35 28 BanII G_rGCy'C Cuts at: 1213 2304 3613 4643 1213 Size: 1091 1309 1030 3169 Fragments arranged by size: 3169 1309 1091 1030 BbeI G_GCGC'C Cuts at: 4033 4281 4033 Size: 248 6351 BbvCI CC'TCA_GC Cuts at: 2560 2560 Size: 6599 BciVI GTATCCnnnnn_n' Cuts at: 429 4497 5258 6599 429 Size: 4068 761 1341 429 Fragments arranged by size: 4068 1341 761 429 BclI T'GATC_A Cuts at: 1436 2183 4119 1436 Size: 747 1936 3916 Fragments arranged by size: 3916 1936 747 BfrBI ATG'CAT Cuts at: 341 439 341 Size: 98 6501 BglI GCCn_nnn'nGGC Cuts at: 1038 2392 1038 Size: 1354 5245 BglII A'GATC_T Cuts at: 3482 4114 6151 3482 Size: 632 2037 3930 Fragments arranged by size: 3930 2037 632 BlpI GC'TnA_GC Cuts at: 3711 3711 Size: 6599 Bme1580I G_kGCm'C Cuts at: 2272 2388 4224 4317 5373 2272 Size: 116 1836 93 1056 3498 Fragments arranged by size: 3498 1836 1056 116 93 BmgBI CAC'GTC Cuts at: 1994 1994 Size: 6599 BmrI ACTGGGnnnn_n' Cuts at: 978 1330 2856 3811 3892 4227 6371 978 Size: 352 1526 955 81 335 2144 1206 Fragments arranged by size: 2144 1526 1206 955 352 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2140 2289 4966 2140 Size: 149 2677 3773 Fragments arranged by size: 3773 2677 149 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 752 1103 1477 1676 2806 3264 3362 3560 752 Size: 351 374 199 1130 458 98 198 3791 Fragments arranged by size: 3791 1130 458 374 351 199 198 98 Bpu10I CC'TnA_GC Cuts at: 1097 1905 2560 1097 Size: 808 655 5136 Fragments arranged by size: 5136 808 655 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1777 3775 4654 5146 5444 1777 Size: 1998 879 492 298 2932 Fragments arranged by size: 2932 1998 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 1054 6303 1054 Size: 5249 1350 BsaAI yAC'GTr Cuts at: 198 215 2152 3952 198 Size: 17 1937 1800 2845 Fragments arranged by size: 2845 1937 1800 17 BsaBI GATnn'nnATC Cuts at: 6 4113 6150 6 Size: 4107 2037 455 Fragments arranged by size: 4107 2037 455 BsaHI Gr'CG_yC Cuts at: 161 4030 4278 4980 6476 161 Size: 3869 248 702 1496 284 Fragments arranged by size: 3869 1496 702 284 248 BsaWI w'CCGG_w Cuts at: 3788 4309 4987 5261 5408 5819 3788 Size: 521 678 274 147 411 4568 Fragments arranged by size: 4568 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1548 1578 2779 2809 3533 3563 1548 Size: 30 1201 30 724 30 4584 Fragments arranged by size: 4584 1201 724 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1183 2505 2537 2577 2815 1183 Size: 1322 32 40 238 4967 Fragments arranged by size: 4967 1322 238 40 32 BseYI C'CCAG_C Cuts at: 884 2615 4024 4782 5359 5855 884 Size: 1731 1409 758 577 496 1628 Fragments arranged by size: 1731 1628 1409 758 577 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 237 1088 2368 237 Size: 851 1280 4468 Fragments arranged by size: 4468 1280 851 BsiEI CG_ry'CG Cuts at: 104 1057 4141 4187 5395 104 Size: 953 3084 46 1208 1308 Fragments arranged by size: 3084 1308 1208 953 46 BsiHKAI G_wGCw'C Cuts at: 134 1387 3079 3613 4391 4581 5373 134 Size: 1253 1692 534 778 190 792 1360 Fragments arranged by size: 1692 1360 1253 792 778 534 190 BsmBI CGTCTCn'nnnn_ Cuts at: 1237 1768 1237 Size: 531 6068 Bsp1286I G_dGCh'C Cuts at: 134 1213 1387 2272 2304 2388 3079 3613 4224 Size: 1079 174 885 32 84 691 534 611 Cuts at: 4224 4317 4391 4581 4643 5373 134 Size: 93 74 190 62 730 1360 Fragments arranged by size: 1360 1079 885 730 691 611 534 190 174 93 84 74 62 32 BspEI T'CCGG_A Cuts at: 5819 5819 Size: 6599 BspHI T'CATG_A Cuts at: 5775 5775 Size: 6599 BspMI ACCTGCnnnn'nnnn_ Cuts at: 3649 3863 4165 4546 3649 Size: 214 302 381 5702 Fragments arranged by size: 5702 381 302 214 BsrBI CCG'CTC Cuts at: 50 1053 4891 4945 6429 6594 50 Size: 1003 3838 54 1484 165 55 Fragments arranged by size: 3838 1484 1003 165 55 54 BsrDI GCAATG_nn' Cuts at: 252 3184 4511 252 Size: 2932 1327 2340 Fragments arranged by size: 2932 2340 1327 BsrFI r'CCGG_y Cuts at: 157 4597 4778 4987 157 Size: 4440 181 209 1769 Fragments arranged by size: 4440 1769 209 181 BssSI C'ACGA_G Cuts at: 2200 4870 5228 2200 Size: 2670 358 3571 Fragments arranged by size: 3571 2670 358 BstAPI GCAn_nnn'nTGC Cuts at: 3976 3976 Size: 6599 BstBI TT'CG_AA Cuts at: 2289 4960 2289 Size: 2671 3928 BstEII G'GTnAC_C Cuts at: 879 879 Size: 6599 BstXI CCAn_nnnn'nTGG Cuts at: 518 518 Size: 6599 BstYI r'GATC_y Cuts at: 1 393 1660 1791 2240 2836 2919 3482 3624 Size: 392 1267 131 449 596 83 563 142 Cuts at: 3624 4091 4114 4449 4695 5707 5793 5805 6151 Size: 467 23 335 246 1012 86 12 346 Cuts at: 6151 1 Size: 449 Fragments arranged by size: 1267 1012 596 563 467 449 449 392 346 335 246 142 131 86 83 23 12 Bsu36I CC'TnA_GG Cuts at: 1034 1302 3290 1034 Size: 268 1988 4343 Fragments arranged by size: 4343 1988 268 BtgI C'CryG_G Cuts at: 107 385 2031 2130 107 Size: 278 1646 99 4576 Fragments arranged by size: 4576 1646 278 99 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 182 1732 4549 4730 4893 182 Size: 1550 2817 181 163 1888 Fragments arranged by size: 2817 1888 1550 181 163 BtsI GCAGTG_nn' Cuts at: 1164 1715 3842 1164 Size: 551 2127 3921 Fragments arranged by size: 3921 2127 551 ClaI AT'CG_AT Cuts at: 3224 3224 Size: 6599 DraI TTT'AAA Cuts at: 2642 3598 2642 Size: 956 5643 DraIII CAC_nnn'GTG Cuts at: 1071 1575 1071 Size: 504 6095 DrdI GACnn_nn'nnGTC Cuts at: 3577 4305 5163 3577 Size: 728 858 5013 Fragments arranged by size: 5013 858 728 EaeI y'GGCC_r Cuts at: 1054 2204 3314 3515 4184 4358 4749 4776 6121 Size: 1150 1110 201 669 174 391 27 1345 Cuts at: 6121 1054 Size: 1532 Fragments arranged by size: 1532 1345 1150 1110 669 391 201 174 27 EagI C'GGCC_G Cuts at: 1054 4184 1054 Size: 3130 3469 EarI CTCTTCn'nnn_ Cuts at: 692 1375 1418 1504 2654 4622 4832 692 Size: 683 43 86 1150 1968 210 2459 Fragments arranged by size: 2459 1968 1150 683 210 86 43 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2866 4807 5117 5263 6409 2866 Size: 1941 310 146 1146 3056 Fragments arranged by size: 3056 1941 1146 310 146 EcoICRI GAG'CTC Cuts at: 3611 3611 Size: 6599 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 271 752 1103 1477 1676 1825 2467 2672 2806 Size: 481 351 374 199 149 642 205 134 Cuts at: 2806 2926 2953 3214 3264 3362 3560 4424 4856 Size: 120 27 261 50 98 198 864 432 Cuts at: 4856 5603 5871 271 Size: 747 268 999 Fragments arranged by size: 999 864 747 642 481 432 374 351 268 261 205 199 198 149 134 120 98 50 27 EcoNI CCTnn'n_nnAGG Cuts at: 952 1033 1132 1301 952 Size: 81 99 169 6250 Fragments arranged by size: 6250 169 99 81 EcoO109I rG'GnC_Cy Cuts at: 943 1809 3286 3738 943 Size: 866 1477 452 3804 Fragments arranged by size: 3804 1477 866 452 EcoRI G'AATT_C Cuts at: 1883 3603 1883 Size: 1720 4879 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2003 3579 4618 2003 Size: 1576 1039 3984 Fragments arranged by size: 3984 1576 1039 FspI TGC'GCA Cuts at: 4380 4380 Size: 6599 HaeII r_GCGC'y Cuts at: 4033 4281 5303 6282 4033 Size: 248 1022 979 4350 Fragments arranged by size: 4350 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 438 470 628 660 1503 1535 2494 2526 2779 Size: 32 158 32 843 32 959 32 253 Cuts at: 2779 2811 3075 3107 3533 3565 4441 4471 4473 Size: 32 264 32 426 32 876 30 2 Cuts at: 4473 4503 438 Size: 30 2534 Fragments arranged by size: 2534 959 876 843 426 264 253 158 32 32 32 32 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 1741 2502 3638 1741 Size: 761 1136 4702 Fragments arranged by size: 4702 1136 761 HindIII A'AGCT_T Cuts at: 2644 3398 2644 Size: 754 5845 KasI G'GCGC_C Cuts at: 4029 4277 4029 Size: 248 6351 KpnI G_GTAC'C Cuts at: 658 1607 3619 658 Size: 949 2012 3638 Fragments arranged by size: 3638 2012 949 MluI A'CGCG_T Cuts at: 6032 6032 Size: 6599 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 488 5270 5454 488 Size: 4782 184 1633 Fragments arranged by size: 4782 1633 184 MscI TGG'CCA Cuts at: 2206 3517 4360 2206 Size: 1311 843 4445 Fragments arranged by size: 4445 1311 843 MslI CAynn'nnrTG Cuts at: 1703 2362 4588 4715 1703 Size: 659 2226 127 3587 Fragments arranged by size: 3587 2226 659 127 MspA1I CmG'CkG Cuts at: 1093 2580 3710 4024 4384 5397 5642 1093 Size: 1487 1130 314 360 1013 245 2050 Fragments arranged by size: 2050 1487 1130 1013 360 314 245 NaeI GCC'GGC Cuts at: 4780 4780 Size: 6599 NarI GG'CG_CC Cuts at: 4030 4278 4030 Size: 248 6351 NcoI C'CATG_G Cuts at: 107 385 2031 2130 107 Size: 278 1646 99 4576 Fragments arranged by size: 4576 1646 278 99 NdeI CA'TA_TG Cuts at: 2172 2172 Size: 6599 NgoMIV G'CCGG_C Cuts at: 4778 4778 Size: 6599 NsiI A_TGCA'T Cuts at: 343 441 343 Size: 98 6501 NspI r_CATG'y Cuts at: 439 3069 3171 3372 3652 4152 5059 439 Size: 2630 102 201 280 500 907 1979 Fragments arranged by size: 2630 1979 907 500 280 201 102 PasI CC'CwG_GG Cuts at: 1128 1128 Size: 6599 PciI A'CATG_T Cuts at: 5055 5055 Size: 6599 PflMI CCAn_nnn'nTGG Cuts at: 1455 1614 1455 Size: 159 6440 PfoI T'CCnGG_A Cuts at: 1080 1448 1080 Size: 368 6231 PmeI GTTT'AAAC Cuts at: 3598 3598 Size: 6599 PmlI CAC'GTG Cuts at: 215 2152 215 Size: 1937 4662 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 500 532 5738 5770 500 Size: 32 5206 32 1329 Fragments arranged by size: 5206 1329 32 32 PpuMI rG'GwC_Cy Cuts at: 943 1809 943 Size: 866 5733 PshAI GACnn'nnGTC Cuts at: 160 1424 160 Size: 1264 5335 PstI C_TGCA'G Cuts at: 3646 4331 3646 Size: 685 5914 PvuI CG_AT'CG Cuts at: 104 104 Size: 6599 PvuII CAG'CTG Cuts at: 2580 4024 4384 2580 Size: 1444 360 4795 Fragments arranged by size: 4795 1444 360 RsrII CG'GwC_CG Cuts at: 4794 4794 Size: 6599 SacI G_AGCT'C Cuts at: 3613 3613 Size: 6599 SalI G'TCGA_C Cuts at: 3636 3636 Size: 6599 SanDI GG'GwC_CC Cuts at: 943 943 Size: 6599 SapI GCTCTTCn'nnn_ Cuts at: 1375 1418 4622 4832 1375 Size: 43 3204 210 3142 Fragments arranged by size: 3204 3142 210 43 SbfI CC_TGCA'GG Cuts at: 3646 3646 Size: 6599 ScaI AGT'ACT Cuts at: 1466 6116 1466 Size: 4650 1949 SexAI A'CCwGG_T Cuts at: 1571 1571 Size: 6599 SfcI C'TryA_G Cuts at: 33 674 2193 3642 4327 5320 5511 33 Size: 641 1519 1449 685 993 191 1121 Fragments arranged by size: 1519 1449 1121 993 685 641 191 SfoI GGC'GCC Cuts at: 4031 4279 4031 Size: 248 6351 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 6599 SmaI CCC'GGG Cuts at: 3621 3621 Size: 6599 SmlI C'TyrA_G Cuts at: 151 1756 3754 4669 5161 5423 151 Size: 1605 1998 915 492 262 1327 Fragments arranged by size: 1998 1605 1327 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 6599 SphI G_CATG'C Cuts at: 439 3069 3652 4152 439 Size: 2630 583 500 2886 Fragments arranged by size: 2886 2630 583 500 StyI C'CwwG_G Cuts at: 107 385 780 2031 2130 2207 3563 3733 4087 Size: 278 395 1251 99 77 1356 170 354 Cuts at: 4087 107 Size: 2619 Fragments arranged by size: 2619 1356 1251 395 354 278 170 99 77 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1033 1120 1726 3422 4182 4315 4774 4983 1033 Size: 87 606 1696 760 133 459 209 2649 Fragments arranged by size: 2649 1696 760 606 459 209 133 87 TatI w'GTAC_w Cuts at: 959 1464 6114 959 Size: 505 4650 1444 Fragments arranged by size: 4650 1444 505 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 225 257 1548 1580 2842 2874 6116 6148 225 Size: 32 1291 32 1262 32 3242 32 676 Fragments arranged by size: 3242 1291 1262 676 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 2985 4396 2985 Size: 1411 5188 XbaI T'CTAG_A Cuts at: 1623 3630 1623 Size: 2007 4592 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1651 3249 3561 4094 1651 Size: 1598 312 533 4156 Fragments arranged by size: 4156 1598 533 312 XmaI C'CCGG_G Cuts at: 3619 3619 Size: 6599 Enzymes that do cut: AccI Acc65I AclI AcuI AflII AflIII AgeI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbvCI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PstI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AfeI AhdI AleI AloI ApaI AscI AvrII BaeI BbsI BcgI BmtI BsiWI BsmI BsrGI BssHII BstZ17I CspCI EcoRV FseI FspAI HpaI MfeI NheI NotI NruI PacI PsiI PspOMI PspXI PsrI SacII SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI