(Linear) (Six-base) MAPSORT of: KIEE2631.seq Check: 8117 from: 1 to: 3348 KIEE2631.orf With 182 enzymes: SgfI * July 8, 2016 11:01 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 724 3348 Size: 724 2624 AccI GT'mk_AC Cuts at: 0 1334 3348 Size: 1334 2014 AclI AA'CG_TT Cuts at: 0 2429 3348 Size: 2429 919 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1161 2113 3081 3348 Size: 1161 952 968 267 Fragments arranged by size: 1161 968 952 267 AfeI AGC'GCT Cuts at: 0 2458 3348 Size: 2458 890 AflIII A'CryG_T Cuts at: 0 1706 3348 Size: 1706 1642 AleI CACnn'nnGTG Cuts at: 0 364 706 730 3157 3348 Size: 364 342 24 2427 191 Fragments arranged by size: 2427 364 342 191 24 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 724 2530 3348 Size: 724 1806 818 Fragments arranged by size: 1806 818 724 AlwNI CAG_nnn'CTG Cuts at: 0 1740 2022 3348 Size: 1740 282 1326 Fragments arranged by size: 1740 1326 282 ApaI G_GGCC'C Cuts at: 0 520 1518 1611 1991 3061 3348 Size: 520 998 93 380 1070 287 Fragments arranged by size: 1070 998 520 380 287 93 ApaLI G'TGCA_C Cuts at: 0 2059 2494 3348 Size: 2059 435 854 Fragments arranged by size: 2059 854 435 AvaI C'yCGr_G Cuts at: 0 48 1539 2287 3191 3271 3348 Size: 48 1491 748 904 80 77 Fragments arranged by size: 1491 904 748 80 77 48 BamHI G'GATC_C Cuts at: 0 1468 3348 Size: 1468 1880 BanI G'GyrC_C Cuts at: 0 292 532 592 700 1300 2137 2189 2336 Size: 292 240 60 108 600 837 52 147 Cuts at: 2336 2537 3348 Size: 201 811 Fragments arranged by size: 837 811 600 292 240 201 147 108 60 52 BanII G_rGCy'C Cuts at: 0 520 1081 1518 1611 1991 2125 3061 3348 Size: 520 561 437 93 380 134 936 287 Fragments arranged by size: 936 561 520 437 380 287 134 93 BbeI G_GCGC'C Cuts at: 0 2193 2340 3348 Size: 2193 147 1008 Fragments arranged by size: 2193 1008 147 BbsI GAAGACnn'nnnn_ Cuts at: 0 765 1167 2719 3348 Size: 765 402 1552 629 Fragments arranged by size: 1552 765 629 402 BbvCI CC'TCA_GC Cuts at: 0 1744 3348 Size: 1744 1604 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1134 1168 2914 2948 3348 Size: 1134 34 1746 34 400 Fragments arranged by size: 1746 1134 400 34 34 BciVI GTATCCnnnnn_n' Cuts at: 0 1925 3348 Size: 1925 1423 BfrBI ATG'CAT Cuts at: 0 2679 2757 3348 Size: 2679 78 591 Fragments arranged by size: 2679 591 78 BglI GCCn_nnn'nGGC Cuts at: 0 913 2170 3348 Size: 913 1257 1178 Fragments arranged by size: 1257 1178 913 Bme1580I G_kGCm'C Cuts at: 0 295 520 562 595 1432 1518 1559 1611 Size: 295 225 42 33 837 86 41 52 Cuts at: 1611 1991 2063 2140 2498 3061 3348 Size: 380 72 77 358 563 287 Fragments arranged by size: 837 563 380 358 295 287 225 86 77 72 52 42 41 33 BmgBI CAC'GTC Cuts at: 0 2360 3348 Size: 2360 988 BmrI ACTGGGnnnn_n' Cuts at: 0 3056 3348 Size: 3056 292 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2478 2527 3348 Size: 2478 49 821 Fragments arranged by size: 2478 821 49 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1095 1779 1999 2028 3348 Size: 1095 684 220 29 1320 Fragments arranged by size: 1320 1095 684 220 29 Bpu10I CC'TnA_GC Cuts at: 0 1744 2453 3348 Size: 1744 709 895 Fragments arranged by size: 1744 895 709 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2152 3348 Size: 2152 1196 BsaI GGTCTCn'nnnn_ Cuts at: 0 515 3348 Size: 515 2833 BsaBI GATnn'nnATC Cuts at: 0 1642 2569 3348 Size: 1642 927 779 Fragments arranged by size: 1642 927 779 BsaHI Gr'CG_yC Cuts at: 0 338 2190 2337 3197 3348 Size: 338 1852 147 860 151 Fragments arranged by size: 1852 860 338 151 147 BsaWI w'CCGG_w Cuts at: 0 2822 3000 3348 Size: 2822 178 348 Fragments arranged by size: 2822 348 178 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1327 1357 3348 Size: 1327 30 1991 Fragments arranged by size: 1991 1327 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 160 163 957 1125 2229 2917 2920 2986 Size: 160 3 794 168 1104 688 3 66 Cuts at: 2986 3063 3348 Size: 77 285 Fragments arranged by size: 1104 794 688 285 168 160 77 66 3 3 BseYI C'CCAG_C Cuts at: 0 624 814 1492 2066 2087 2165 2938 2947 Size: 624 190 678 574 21 78 773 9 Cuts at: 2947 3348 Size: 401 Fragments arranged by size: 773 678 624 574 401 190 78 21 9 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 295 1748 1753 2932 3348 Size: 295 1453 5 1179 416 Fragments arranged by size: 1453 1179 416 295 5 BsiEI CG_ry'CG Cuts at: 0 3158 3324 3348 Size: 3158 166 24 Fragments arranged by size: 3158 166 24 BsiHKAI G_wGCw'C Cuts at: 0 713 1081 2063 2498 2937 3290 3348 Size: 713 368 982 435 439 353 58 Fragments arranged by size: 982 713 439 435 368 353 58 BsmBI CGTCTCn'nnnn_ Cuts at: 0 925 2866 3348 Size: 925 1941 482 Fragments arranged by size: 1941 925 482 Bsp1286I G_dGCh'C Cuts at: 0 295 520 562 595 713 1081 1432 1518 Size: 295 225 42 33 118 368 351 86 Cuts at: 1518 1559 1611 1991 2063 2125 2140 2498 2937 Size: 41 52 380 72 62 15 358 439 Cuts at: 2937 3061 3290 3348 Size: 124 229 58 Fragments arranged by size: 439 380 368 358 351 295 229 225 124 118 86 72 62 58 52 42 41 33 15 BspEI T'CCGG_A Cuts at: 0 2822 3000 3348 Size: 2822 178 348 Fragments arranged by size: 2822 348 178 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 218 320 724 1557 3348 Size: 218 102 404 833 1791 Fragments arranged by size: 1791 833 404 218 102 BsrBI CCG'CTC Cuts at: 0 1259 2775 2781 3348 Size: 1259 1516 6 567 Fragments arranged by size: 1516 1259 567 6 BsrDI GCAATG_nn' Cuts at: 0 374 1649 1868 3249 3348 Size: 374 1275 219 1381 99 Fragments arranged by size: 1381 1275 374 219 99 BsrFI r'CCGG_y Cuts at: 0 270 3348 Size: 270 3078 BsrGI T'GTAC_A Cuts at: 0 581 3348 Size: 581 2767 BssSI C'ACGA_G Cuts at: 0 1420 2810 3348 Size: 1420 1390 538 Fragments arranged by size: 1420 1390 538 BstAPI GCAn_nnn'nTGC Cuts at: 0 652 1740 3348 Size: 652 1088 1608 Fragments arranged by size: 1608 1088 652 BstEII G'GTnAC_C Cuts at: 0 868 936 2418 3348 Size: 868 68 1482 930 Fragments arranged by size: 1482 930 868 68 BstXI CCAn_nnnn'nTGG Cuts at: 0 168 513 1451 3348 Size: 168 345 938 1897 Fragments arranged by size: 1897 938 345 168 BstYI r'GATC_y Cuts at: 0 1025 1468 2619 2741 2786 2997 3348 Size: 1025 443 1151 122 45 211 351 Fragments arranged by size: 1151 1025 443 351 211 122 45 Bsu36I CC'TnA_GG Cuts at: 0 1270 2978 3348 Size: 1270 1708 370 Fragments arranged by size: 1708 1270 370 BtgI C'CryG_G Cuts at: 0 536 629 888 1450 1578 1902 3312 3348 Size: 536 93 259 562 128 324 1410 36 Fragments arranged by size: 1410 562 536 324 259 128 93 36 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 47 1299 1940 3348 Size: 47 1252 641 1408 Fragments arranged by size: 1408 1252 641 47 BtsI GCAGTG_nn' Cuts at: 0 1736 2651 3348 Size: 1736 915 697 Fragments arranged by size: 1736 915 697 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 237 272 3348 Size: 237 35 3076 Fragments arranged by size: 3076 237 35 EaeI y'GGCC_r Cuts at: 0 194 437 632 722 803 1136 1310 1441 Size: 194 243 195 90 81 333 174 131 Cuts at: 1441 1575 2400 3155 3321 3348 Size: 134 825 755 166 27 Fragments arranged by size: 825 755 333 243 195 194 174 166 134 131 90 81 27 EagI C'GGCC_G Cuts at: 0 3155 3321 3348 Size: 3155 166 27 Fragments arranged by size: 3155 166 27 EarI CTCTTCn'nnn_ Cuts at: 0 230 352 625 2171 2864 2885 3348 Size: 230 122 273 1546 693 21 463 Fragments arranged by size: 1546 693 463 273 230 122 21 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 2568 2842 3348 Size: 2568 274 506 Fragments arranged by size: 2568 506 274 EcoICRI GAG'CTC Cuts at: 0 1079 3348 Size: 1079 2269 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1095 1161 1779 1999 2028 2113 3081 3348 Size: 1095 66 618 220 29 85 968 267 Fragments arranged by size: 1095 968 618 267 220 85 66 29 EcoNI CCTnn'n_nnAGG Cuts at: 0 214 1090 1528 1663 2197 3348 Size: 214 876 438 135 534 1151 Fragments arranged by size: 1151 876 534 438 214 135 EcoO109I rG'GnC_Cy Cuts at: 0 279 954 1266 1361 1514 1515 1608 1987 Size: 279 675 312 95 153 1 93 379 Cuts at: 1987 1988 2106 2283 3057 3058 3276 3348 Size: 1 118 177 774 1 218 72 Fragments arranged by size: 774 675 379 312 279 218 177 153 118 95 93 72 1 1 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 849 3111 3348 Size: 849 2262 237 Fragments arranged by size: 2262 849 237 HaeII r_GCGC'y Cuts at: 0 2193 2340 2460 3348 Size: 2193 147 120 888 Fragments arranged by size: 2193 888 147 120 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1174 1206 1923 1955 2245 2277 2804 2817 Size: 1174 32 717 32 290 32 527 13 Cuts at: 2817 2836 2849 2910 2942 3348 Size: 19 13 61 32 406 Fragments arranged by size: 1174 717 527 406 290 61 32 32 32 32 19 13 13 HincII GTy'rAC Cuts at: 0 756 3348 Size: 756 2592 HindIII A'AGCT_T Cuts at: 0 3202 3348 Size: 3202 146 KasI G'GCGC_C Cuts at: 0 2189 2336 3348 Size: 2189 147 1012 Fragments arranged by size: 2189 1012 147 MfeI C'AATT_G Cuts at: 0 455 3348 Size: 455 2893 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 2267 3158 3348 Size: 2267 891 190 Fragments arranged by size: 2267 891 190 MscI TGG'CCA Cuts at: 0 196 634 724 805 1312 1443 1577 2402 Size: 196 438 90 81 507 131 134 825 Cuts at: 2402 3348 Size: 946 Fragments arranged by size: 946 825 507 438 196 134 131 90 81 MslI CAynn'nnrTG Cuts at: 0 298 304 364 445 706 718 730 948 Size: 298 6 60 81 261 12 12 218 Cuts at: 948 1169 1449 1711 2512 2608 3157 3348 Size: 221 280 262 801 96 549 191 Fragments arranged by size: 801 549 298 280 262 261 221 218 191 96 81 60 12 12 6 MspA1I CmG'CkG Cuts at: 0 2019 2070 2091 2169 2398 2916 2942 3314 Size: 2019 51 21 78 229 518 26 372 Cuts at: 3314 3348 Size: 34 Fragments arranged by size: 2019 518 372 229 78 51 34 26 21 NarI GG'CG_CC Cuts at: 0 2190 2337 3348 Size: 2190 147 1011 Fragments arranged by size: 2190 1011 147 NcoI C'CATG_G Cuts at: 0 629 888 1450 1578 3348 Size: 629 259 562 128 1770 Fragments arranged by size: 1770 629 562 259 128 NotI GC'GGCC_GC Cuts at: 0 3321 3348 Size: 3321 27 NsiI A_TGCA'T Cuts at: 0 2681 2759 3348 Size: 2681 78 589 Fragments arranged by size: 2681 589 78 NspI r_CATG'y Cuts at: 0 690 1710 2511 3150 3222 3348 Size: 690 1020 801 639 72 126 Fragments arranged by size: 1020 801 690 639 126 72 PasI CC'CwG_GG Cuts at: 0 216 2965 3348 Size: 216 2749 383 Fragments arranged by size: 2749 383 216 PciI A'CATG_T Cuts at: 0 1706 3348 Size: 1706 1642 PflMI CCAn_nnn'nTGG Cuts at: 0 526 2006 2055 3348 Size: 526 1480 49 1293 Fragments arranged by size: 1480 1293 526 49 PfoI T'CCnGG_A Cuts at: 0 2384 3348 Size: 2384 964 PpuMI rG'GwC_Cy Cuts at: 0 1266 3276 3348 Size: 1266 2010 72 Fragments arranged by size: 2010 1266 72 PspOMI G'GGCC_C Cuts at: 0 516 1514 1607 1987 3057 3348 Size: 516 998 93 380 1070 291 Fragments arranged by size: 1070 998 516 380 291 93 PstI C_TGCA'G Cuts at: 0 326 1502 1532 1568 2300 3348 Size: 326 1176 30 36 732 1048 Fragments arranged by size: 1176 1048 732 326 36 30 PvuII CAG'CTG Cuts at: 0 2019 2070 2091 2398 2916 2942 3348 Size: 2019 51 21 307 518 26 406 Fragments arranged by size: 2019 518 406 307 51 26 21 SacI G_AGCT'C Cuts at: 0 1081 3348 Size: 1081 2267 SacII CC_GC'GG Cuts at: 0 3315 3348 Size: 3315 33 SbfI CC_TGCA'GG Cuts at: 0 1568 3348 Size: 1568 1780 SfcI C'TryA_G Cuts at: 0 322 1498 1528 1564 2296 3348 Size: 322 1176 30 36 732 1052 Fragments arranged by size: 1176 1052 732 322 36 30 SfoI GGC'GCC Cuts at: 0 2191 2338 3348 Size: 2191 147 1010 Fragments arranged by size: 2191 1010 147 SmaI CCC'GGG Cuts at: 0 50 3193 3348 Size: 50 3143 155 Fragments arranged by size: 3143 155 50 SmlI C'TyrA_G Cuts at: 0 1539 2131 3271 3348 Size: 1539 592 1140 77 Fragments arranged by size: 1539 1140 592 77 SphI G_CATG'C Cuts at: 0 2511 3150 3348 Size: 2511 639 198 Fragments arranged by size: 2511 639 198 StyI C'CwwG_G Cuts at: 0 65 629 888 1091 1450 1578 3026 3348 Size: 65 564 259 203 359 128 1448 322 Fragments arranged by size: 1448 564 359 322 259 203 128 65 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 354 730 858 1767 3348 Size: 354 376 128 909 1581 Fragments arranged by size: 1581 909 376 354 128 TatI w'GTAC_w Cuts at: 0 581 1889 3348 Size: 581 1308 1459 Fragments arranged by size: 1459 1308 581 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 833 865 3348 Size: 833 32 2483 Fragments arranged by size: 2483 833 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 585 1036 3348 Size: 585 451 2312 Fragments arranged by size: 2312 585 451 XhoI C'TCGA_G Cuts at: 0 1539 3271 3348 Size: 1539 1732 77 Fragments arranged by size: 1732 1539 77 XmaI C'CCGG_G Cuts at: 0 48 3191 3348 Size: 48 3143 157 Fragments arranged by size: 3143 157 48 XmnI GAAnn'nnTTC Cuts at: 0 2218 3348 Size: 2218 1130 Enzymes that do cut: AarI AccI AclI AcuI AfeI AflIII AleI AlfI AlwNI ApaI ApaLI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BfrBI BglI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HincII HindIII KasI MfeI MmeI MscI MslI MspA1I NarI NcoI NotI NsiI NspI PasI PciI PflMI PfoI PpuMI PspOMI PstI PvuII SacI SacII SbfI SfcI SfoI SmaI SmlI SphI StyI TaqII TatI TstI XcmI XhoI XmaI XmnI Enzymes that do not cut: AatII Acc65I AflII AgeI AhdI AloI ApoI AscI AseI AsiSI AvrII BaeI BclI BglII BlpI BmtI BsaAI BsiWI BsmI BspHI BssHII BstBI BstZ17I ClaI DraI DraIII DrdI EcoRI EcoRV FseI FspI FspAI HpaI KpnI MluI NaeI NdeI NgoMIV NheI NruI PacI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SalI SanDI SapI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SrfI SspI StuI SwaI Tth111I XbaI ZraI