Restriction map for pF1KE2580
##################################
# NO CUT ENZYMES (  114 enzymes) #
##################################

AatII       AccII       AccIII      AclI        AcyI        
AflII       AgeI        AhdI        Alw44I      AocI        
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        Asp700I     AspEI       AspI        AvaI        
AviII       AvrII       BbeI        BbrPI       BciVI       
BcnI        BfrI        BlnI        BlpI        Bpu1102I    
BsaBI       BsaHI       BsaWI       BseAI       BsiWI       
BsmBI       BsoBI       BspDI       BspEI       BsrBI       
BsrFI       BssHII      Bst1107I    BstAPI      BstEII      
BstPI       BstUI       BstZ17I     Bsu36I      CelII       
CfoI        Cfr10I      ClaI        CpoI        DraIII      
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoO65I     EcoT22I     FseI        FspI        
HaeII       HhaI        Hin1I       HinP1I      HpaI        
KasI        KspI        MamI        MluI        MroI        
MvnI        NaeI        NarI        NciI        NcoI        
NgoMIV      NheI        NotI        NruI        NsiI        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshBI       Psp1406I    
PvuI        RsrII       SacII       SapI        SbfI        
ScaI        SexAI       SfiI        SfoI        SgrAI       
SmaI        SphI        Sse8387I    SwaI        Tth111I     
XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   28 enzymes) #
###################################

Acc65I       2015  GGTACC               G^GTACC
AflIII       1772  ACATGT               A^C[GA][CT]GT
Asp718I      2015  GGTACC               G^GTACC
BglI          793  GCCAAAAGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI         1448  CTGGAG               CTGGAG(16/14)
BsaI         3116  GAGACC               GGTCTC(1/5)
BsiEI         205  CGGTCG               CG[GA][CT]^CG
BsmI          181  GAATGC               GAATGC(1/-1)
BspLU11I     1772  ACATGT               A^CATGT
BssSI        3172  CACGAG               CACGAG(-5/-1)
BstBI        2402  TTCGAA               TT^CGAA
DraII        1610  AGGGCCT              [GA]G^G[ACGT]CC[CT]
DrdI         2296  GACCTGGCTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI         2690  CCACGG               C^C[GA][CT]GG
EcoO109I     1610  AGGGCCT              [GA]G^G[ACGT]CC[CT]
EcoT14I      1871  CCAAGG               C^C[AT][AT]GG
HindIII      1424  AAGCTT               A^AGCTT
KpnI         2015  GGTACC               GGTAC^C
NdeI         2465  CATATG               CA^TATG
NspV         2402  TTCGAA               TT^CGAA
PshAI        1570  GACTTGGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI         2674  GAGCTC               GAGCT^C
SalI         2916  GTCGAC               G^TCGAC
SfuI         2402  TTCGAA               TT^CGAA
SpeI         2575  ACTAGT               A^CTAGT
StyI         1871  CCAAGG               C^C[AT][AT]GG
XbaI         3429  TCTAGA               T^CTAGA
XcmI         2690  CCACGGCTGCCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   41 enzymes) #
###################################

AccI          715  GTAGAC               GT^[AC][GT]AC
             2916  GTCGAC              
AlwNI        3007  CAGACGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             3187  CAGCAGCTG           
AspHI        2674  GAGCTC               G[AT]GC[AT]^C
             3230  GAGCAC              
BalI            2  TGGCCA               TGG^CCA
             2346  TGGCCA              
BamHI         225  GGATCC               G^GATCC
             2354  GGATCC              
BanI         2015  GGTACC               G^G[CT][GA]CC
             2155  GGCACC              
BanII         598  GAGCCC               G[GA]GC[CT]^C
             2674  GAGCTC              
BbvCI        3126  CCTCAGC              CCTCAGC(-5/-2)
             3097  GCTGAGG             
BclI         1703  TGATCA               T^GATCA
             3080  TGATCA              
BsaAI        2161  TACGTA               [CT]AC^GT[GA]
             2201  TACGTA              
BsiHKAI      2674  GAGCTC               G[AT]GC[AT]^C
             3230  GAGCAC              
BsmFI        3201  GTCCC                GGGAC(10/14)
             3483  GTCCC               
Bsp1407I     1034  TGTACA               T^GTACA
             1138  TGTACA              
BspHI        2036  TCATGA               T^CATGA
             2922  TCATGA              
BspMI        3119  ACCTGC               ACCTGC(4/8)
             2115  GCAGGT              
BsrGI        1034  TGTACA               T^GTACA
             1138  TGTACA              
BstXI         230  CCAAAATCTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3389  CCAAAGAAATGG        
DraI         2793  TTTAAA               TTT^AAA
             2823  TTTAAA              
EaeI            2  TGGCCA               [CT]^GGCC[GA]
             2346  TGGCCA              
EcoNI        1149  CCTTTTTAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             2934  CCTAGAGAAGG         
FbaI         1703  TGATCA               T^GATCA
             3080  TGATCA              
HapII        2293  CCGG                 C^CGG
             2381  CCGG                
HgaI         3009  GACGC                GACGC(5/10)
             1601  GCGTC               
HincII       2881  GTTGAC               GT[CT]^[GA]AC
             2916  GTCGAC              
HindII       2881  GTTGAC               GT[CT]^[GA]AC
             2916  GTCGAC              
HpaII        2293  CCGG                 C^CGG
             2381  CCGG                
MfeI         1823  CAATTG               C^AATTG
             1975  CAATTG              
MluNI           2  TGGCCA               TGG^CCA
             2346  TGGCCA              
MscI            2  TGGCCA               TGG^CCA
             2346  TGGCCA              
MslI          813  CATTGAGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             2223  CATGGTTATG          
MspA1I       1417  CAGCTG               C[AC]G^C[GT]G
             3190  CAGCTG              
MspI         2293  CCGG                 C^CGG
             2381  CCGG                
MunI         1823  CAATTG               C^AATTG
             1975  CAATTG              
PflMI         936  CCAAGAAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              948  CCATCCTCTGG         
PstI          300  CTGCAG               CTGCA^G
             1233  CTGCAG              
PvuII        1417  CAGCTG               CAG^CTG
             3190  CAGCTG              
RcaI         2036  TCATGA               T^CATGA
             2922  TCATGA              
SnaBI        2161  TACGTA               TAC^GTA
             2201  TACGTA              
SspBI        1034  TGTACA               T^GTACA
             1138  TGTACA              
StuI          381  AGGCCT               AGG^CCT
              852  AGGCCT              
Van91I        936  CCAAGAAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              948  CCATCCTCTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   75 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        AvaII       BbsI        BbvI        BcgI        
BfaI        BglII       BmyI        BpuAI       BsaJI       
BseRI       BsgI        BsiYI       BslI        BsmAI       
Bsp1286I    BsrDI       BsrI        BssKI       BstF5I      
BstNI       BstYI       Cac8I       Cfr13I      DdeI        
DpnII       EarI        EcoRI       EcoRII      EcoRV       
FauI        Fnu4HI      FokI        HaeIII      HinfI       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MvaI        MwoI        NdeII       NlaIII      
NlaIV       NspI        PleI        RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
SspI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     XhoII       
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