(Circular) (Six-base) MAPSORT of: pF1KE2580.seq Check: 9550 from: 1 to: 6695 pF1KE2580 6695 bp With 182 enzymes: SgfI * July 27, 2015 09:05 .. AccI GT'mk_AC Cuts at: 824 3025 3733 824 Size: 2201 708 3786 Fragments arranged by size: 3786 2201 708 Acc65I G'GTAC_C Cuts at: 2123 3711 2123 Size: 1588 5107 AclI AA'CG_TT Cuts at: 3689 6535 3689 Size: 2846 3849 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 498 1529 4520 4952 5699 5967 498 Size: 1031 2991 432 747 268 1226 Fragments arranged by size: 2991 1226 1031 747 432 268 AflIII A'CryG_T Cuts at: 1880 4047 5151 6128 1880 Size: 2167 1104 977 2447 Fragments arranged by size: 2447 2167 1104 977 AgeI A'CCGG_T Cuts at: 5083 5083 Size: 6695 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1465 2229 3349 1465 Size: 764 1120 4811 Fragments arranged by size: 4811 1120 764 AlwNI CAG_nnn'CTG Cuts at: 3120 3300 4000 5567 3120 Size: 180 700 1567 4248 Fragments arranged by size: 4248 1567 700 180 ApaLI G'TGCA_C Cuts at: 5465 5465 Size: 6695 ApoI r'AATT_y Cuts at: 124 596 878 1645 1651 1936 2098 2317 2626 Size: 472 282 767 6 285 162 219 309 Cuts at: 2626 2925 3110 3541 3699 6048 6304 124 Size: 299 185 431 158 2349 256 515 Fragments arranged by size: 2349 767 515 472 431 309 299 285 282 256 219 185 162 158 6 AseI AT'TA_AT Cuts at: 20 20 Size: 6695 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 6695 AvaI C'yCGr_G Cuts at: 3715 3715 Size: 6695 BamHI G'GATC_C Cuts at: 333 2462 3720 333 Size: 2129 1258 3308 Fragments arranged by size: 3308 2129 1258 BanI G'GyrC_C Cuts at: 2123 2263 3711 4125 4373 4408 2123 Size: 140 1448 414 248 35 4410 Fragments arranged by size: 4410 1448 414 248 140 35 BanII G_rGCy'C Cuts at: 710 2786 3709 4739 710 Size: 2076 923 1030 2666 Fragments arranged by size: 2666 2076 1030 923 BbeI G_GCGC'C Cuts at: 4129 4377 4129 Size: 248 6447 BbsI GAAGACnn'nnnn_ Cuts at: 670 1011 1121 1214 1877 1927 2873 670 Size: 341 110 93 663 50 946 4492 Fragments arranged by size: 4492 946 663 341 110 93 50 BbvCI CC'TCA_GC Cuts at: 3206 3235 3206 Size: 29 6666 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1098 1132 1948 1982 3595 3629 1098 Size: 34 816 34 1613 34 4164 Fragments arranged by size: 4164 1613 816 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4593 5354 6695 4593 Size: 761 1341 4593 Fragments arranged by size: 4593 1341 761 BclI T'GATC_A Cuts at: 1811 3188 4215 1811 Size: 1377 1027 4291 Fragments arranged by size: 4291 1377 1027 BglI GCCn_nnn'nGGC Cuts at: 907 907 Size: 6695 BglII A'GATC_T Cuts at: 420 777 1383 4210 6247 420 Size: 357 606 2827 2037 868 Fragments arranged by size: 2827 2037 868 606 357 BlpI GC'TnA_GC Cuts at: 3807 3807 Size: 6695 Bme1580I G_kGCm'C Cuts at: 2705 4320 4413 5469 2705 Size: 1615 93 1056 3931 Fragments arranged by size: 3931 1615 1056 93 BmgBI CAC'GTC Cuts at: 2700 2700 Size: 6695 BmrI ACTGGGnnnn_n' Cuts at: 677 1009 2699 3907 3988 4323 6467 677 Size: 332 1690 1208 81 335 2144 905 Fragments arranged by size: 2144 1690 1208 905 335 332 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5062 5062 Size: 6695 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1577 1577 Size: 6695 Bpu10I CC'TnA_GC Cuts at: 3206 3235 3206 Size: 29 6666 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 532 649 2436 2837 3871 4750 5242 5540 532 Size: 117 1787 401 1034 879 492 298 1687 Fragments arranged by size: 1787 1687 1034 879 492 401 298 117 BsaI GGTCTCn'nnnn_ Cuts at: 3218 6399 3218 Size: 3181 3514 BsaAI yAC'GTr Cuts at: 2271 2311 4048 2271 Size: 40 1737 4918 Fragments arranged by size: 4918 1737 40 BsaBI GATnn'nnATC Cuts at: 6 4209 6246 6 Size: 4203 2037 455 Fragments arranged by size: 4203 2037 455 BsaHI Gr'CG_yC Cuts at: 4126 4374 5076 6572 4126 Size: 248 702 1496 4249 Fragments arranged by size: 4249 1496 702 248 BsaWI w'CCGG_w Cuts at: 3884 4405 5083 5357 5504 5915 3884 Size: 521 678 274 147 411 4664 Fragments arranged by size: 4664 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1139 1169 1976 2006 2585 2615 1139 Size: 30 807 30 579 30 5219 Fragments arranged by size: 5219 807 579 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 202 1618 1642 3223 202 Size: 1416 24 1581 3674 Fragments arranged by size: 3674 1581 1416 24 BseYI C'CCAG_C Cuts at: 1190 1572 1823 3293 4120 4878 5455 5951 1190 Size: 382 251 1470 827 758 577 496 1934 Fragments arranged by size: 1934 1470 827 758 577 496 382 251 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 2242 2964 3214 2242 Size: 722 250 5723 Fragments arranged by size: 5723 722 250 BsiEI CG_ry'CG Cuts at: 104 316 4237 4283 5491 104 Size: 212 3921 46 1208 1308 Fragments arranged by size: 3921 1308 1208 212 46 BsiHKAI G_wGCw'C Cuts at: 2786 3342 3709 4487 4677 5469 2786 Size: 556 367 778 190 792 4012 Fragments arranged by size: 4012 792 778 556 367 190 BsmI GAATG_Cn' Cuts at: 295 295 Size: 6695 Bsp1286I G_dGCh'C Cuts at: 710 2705 2786 3342 3709 4320 4413 4487 4677 Size: 1995 81 556 367 611 93 74 190 Cuts at: 4677 4739 5469 710 Size: 62 730 1936 Fragments arranged by size: 1995 1936 730 611 556 367 190 93 81 74 62 BspEI T'CCGG_A Cuts at: 5915 5915 Size: 6695 BspHI T'CATG_A Cuts at: 2144 3030 5871 2144 Size: 886 2841 2968 Fragments arranged by size: 2968 2841 886 BspMI ACCTGCnnnn'nnnn_ Cuts at: 2214 3236 3745 3959 4261 4642 2214 Size: 1022 509 214 302 381 4267 Fragments arranged by size: 4267 1022 509 381 302 214 BsrBI CCG'CTC Cuts at: 50 4987 5041 6525 6690 50 Size: 4937 54 1484 165 55 Fragments arranged by size: 4937 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 484 917 1247 3059 4607 484 Size: 433 330 1812 1548 2572 Fragments arranged by size: 2572 1812 1548 433 330 BsrFI r'CCGG_y Cuts at: 4693 4874 5083 4693 Size: 181 209 6305 Fragments arranged by size: 6305 209 181 BsrGI T'GTAC_A Cuts at: 1142 1246 1142 Size: 104 6591 BssSI C'ACGA_G Cuts at: 3280 4966 5324 3280 Size: 1686 358 4651 Fragments arranged by size: 4651 1686 358 BstAPI GCAn_nnn'nTGC Cuts at: 4072 4072 Size: 6695 BstBI TT'CG_AA Cuts at: 2511 5056 2511 Size: 2545 4150 BstXI CCAn_nnnn'nTGG Cuts at: 345 3504 345 Size: 3159 3536 BstYI r'GATC_y Cuts at: 1 333 420 777 861 1383 1395 2462 2616 Size: 332 87 357 84 522 12 1067 154 Cuts at: 2616 3720 4187 4210 4545 4791 5803 5889 5901 Size: 1104 467 23 335 246 1012 86 12 Cuts at: 5901 6247 1 Size: 346 449 Fragments arranged by size: 1104 1067 1012 522 467 449 357 346 335 332 246 154 87 86 84 23 12 12 BtgI C'CryG_G Cuts at: 107 2798 107 Size: 2691 4004 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2092 4645 4826 4989 2092 Size: 2553 181 163 3798 Fragments arranged by size: 3798 2553 181 163 BtsI GCAGTG_nn' Cuts at: 417 1338 2976 3250 3593 3938 417 Size: 921 1638 274 343 345 3174 Fragments arranged by size: 3174 1638 921 345 343 274 DraI TTT'AAA Cuts at: 2903 2933 3694 2903 Size: 30 761 5904 Fragments arranged by size: 5904 761 30 DrdI GACnn_nn'nnGTC Cuts at: 2410 4401 5259 2410 Size: 1991 858 3846 Fragments arranged by size: 3846 1991 858 EaeI y'GGCC_r Cuts at: 110 2454 4280 4454 4845 4872 6217 110 Size: 2344 1826 174 391 27 1345 588 Fragments arranged by size: 2344 1826 1345 588 391 174 27 EagI C'GGCC_G Cuts at: 4280 4280 Size: 6695 EarI CTCTTCn'nnn_ Cuts at: 226 1595 2363 4718 4928 226 Size: 1369 768 2355 210 1993 Fragments arranged by size: 2355 1993 1369 768 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 408 4903 5213 5359 6505 408 Size: 4495 310 146 1146 598 Fragments arranged by size: 4495 1146 598 310 146 EcoICRI GAG'CTC Cuts at: 2784 3707 2784 Size: 923 5772 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 498 1529 1577 4520 4952 5699 5967 498 Size: 1031 48 2943 432 747 268 1226 Fragments arranged by size: 2943 1226 1031 747 432 268 48 EcoNI CCTnn'n_nnAGG Cuts at: 1261 3046 1261 Size: 1785 4910 EcoO109I rG'GnC_Cy Cuts at: 1719 3834 1719 Size: 2115 4580 EcoRI G'AATT_C Cuts at: 1645 2317 3541 3699 1645 Size: 672 1224 158 4641 Fragments arranged by size: 4641 1224 672 158 EcoRV GAT'ATC Cuts at: 991 1254 2494 2598 991 Size: 263 1240 104 5088 Fragments arranged by size: 5088 1240 263 104 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 669 2907 3544 4714 669 Size: 2238 637 1170 2650 Fragments arranged by size: 2650 2238 1170 637 FspI TGC'GCA Cuts at: 4476 4476 Size: 6695 HaeII r_GCGC'y Cuts at: 4129 4377 5399 6378 4129 Size: 248 1022 979 4446 Fragments arranged by size: 4446 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 178 210 744 776 1139 1171 1976 2008 2161 Size: 32 534 32 363 32 805 32 153 Cuts at: 2161 2193 2596 2628 3114 3121 3146 3153 4537 Size: 32 403 32 486 7 25 7 1384 Cuts at: 4537 4567 4569 4599 178 Size: 30 2 30 2274 Fragments arranged by size: 2274 1384 805 534 486 403 363 153 32 32 32 32 32 32 30 30 25 7 7 2 HincII GTy'rAC Cuts at: 2991 3026 3734 2991 Size: 35 708 5952 Fragments arranged by size: 5952 708 35 HindIII A'AGCT_T Cuts at: 1532 1532 Size: 6695 KasI G'GCGC_C Cuts at: 4125 4373 4125 Size: 248 6447 KpnI G_GTAC'C Cuts at: 2127 3715 2127 Size: 1588 5107 MfeI C'AATT_G Cuts at: 1931 2083 1931 Size: 152 6543 MluI A'CGCG_T Cuts at: 6128 6128 Size: 6695 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 345 5366 5550 345 Size: 5021 184 1490 Fragments arranged by size: 5021 1490 184 MscI TGG'CCA Cuts at: 112 2456 4456 112 Size: 2344 2000 2351 Fragments arranged by size: 2351 2344 2000 MslI CAynn'nnrTG Cuts at: 925 2335 4684 4811 925 Size: 1410 2349 127 2809 Fragments arranged by size: 2809 2349 1410 127 MspA1I CmG'CkG Cuts at: 1527 3300 3806 4120 4480 5493 5738 1527 Size: 1773 506 314 360 1013 245 2484 Fragments arranged by size: 2484 1773 1013 506 360 314 245 NaeI GCC'GGC Cuts at: 4876 4876 Size: 6695 NarI GG'CG_CC Cuts at: 4126 4374 4126 Size: 248 6447 NcoI C'CATG_G Cuts at: 107 107 Size: 6695 NdeI CA'TA_TG Cuts at: 2574 2574 Size: 6695 NgoMIV G'CCGG_C Cuts at: 4874 4874 Size: 6695 NspI r_CATG'y Cuts at: 545 1884 1930 2013 2947 3748 4248 5155 545 Size: 1339 46 83 934 801 500 907 2085 Fragments arranged by size: 2085 1339 934 907 801 500 83 46 PciI A'CATG_T Cuts at: 1880 5151 1880 Size: 3271 3424 PflMI CCAn_nnn'nTGG Cuts at: 1050 1062 1050 Size: 12 6683 PmeI GTTT'AAAC Cuts at: 3694 3694 Size: 6695 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5834 5866 5834 Size: 32 6663 PshAI GACnn'nnGTC Cuts at: 1682 1682 Size: 6695 PsiI TTA'TAA Cuts at: 1763 1877 1949 1763 Size: 114 72 6509 Fragments arranged by size: 6509 114 72 PstI C_TGCA'G Cuts at: 412 1345 3742 4427 412 Size: 933 2397 685 2680 Fragments arranged by size: 2680 2397 933 685 PvuI CG_AT'CG Cuts at: 104 104 Size: 6695 PvuII CAG'CTG Cuts at: 1527 3300 4120 4480 1527 Size: 1773 820 360 3742 Fragments arranged by size: 3742 1773 820 360 RsrII CG'GwC_CG Cuts at: 4890 4890 Size: 6695 SacI G_AGCT'C Cuts at: 2786 3709 2786 Size: 923 5772 SalI G'TCGA_C Cuts at: 3024 3732 3024 Size: 708 5987 SapI GCTCTTCn'nnn_ Cuts at: 4718 4928 4718 Size: 210 6485 SbfI CC_TGCA'GG Cuts at: 3742 3742 Size: 6695 ScaI AGT'ACT Cuts at: 6212 6212 Size: 6695 SfcI C'TryA_G Cuts at: 33 408 1341 1730 3738 4423 5416 5607 33 Size: 375 933 389 2008 685 993 191 1121 Fragments arranged by size: 2008 1121 993 933 685 389 375 191 SfoI GGC'GCC Cuts at: 4127 4375 4127 Size: 248 6447 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 6695 SmaI CCC'GGG Cuts at: 3717 3717 Size: 6695 SmlI C'TyrA_G Cuts at: 547 664 2415 2852 3850 4765 5257 5519 547 Size: 117 1751 437 998 915 492 262 1723 Fragments arranged by size: 1751 1723 998 915 492 437 262 117 SnaBI TAC'GTA Cuts at: 2271 2311 2271 Size: 40 6655 SpeI A'CTAG_T Cuts at: 67 2683 67 Size: 2616 4079 SphI G_CATG'C Cuts at: 3748 4248 3748 Size: 500 6195 SspI AAT'ATT Cuts at: 1375 1869 2559 1375 Size: 494 690 5511 Fragments arranged by size: 5511 690 494 StuI AGG'CCT Cuts at: 491 962 491 Size: 471 6224 StyI C'CwwG_G Cuts at: 107 1979 3829 4183 107 Size: 1872 1850 354 2619 Fragments arranged by size: 2619 1872 1850 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 302 1894 4278 4411 4870 5079 302 Size: 1592 2384 133 459 209 1918 Fragments arranged by size: 2384 1918 1592 459 209 133 TatI w'GTAC_w Cuts at: 1142 1246 1358 6210 1142 Size: 104 112 4852 1627 Fragments arranged by size: 4852 1627 112 104 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 387 419 2585 2617 6212 6244 387 Size: 32 2166 32 3595 32 838 Fragments arranged by size: 3595 2166 838 32 32 32 Tth111I GACn'n_nGTC Cuts at: 4492 4492 Size: 6695 XbaI T'CTAG_A Cuts at: 3537 3726 3537 Size: 189 6506 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2805 4190 2805 Size: 1385 5310 XmaI C'CCGG_G Cuts at: 3715 3715 Size: 6695 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AleI AloI ApaI AscI AvrII BaeI BfrBI BmtI BsiWI BsmBI BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII FseI FspAI HpaI NheI NotI NruI NsiI PacI PasI PfoI PmlI PpuMI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SrfI SwaI XhoI XmnI ZraI