(Linear) (Six-base) MAPSORT of: KIEE2589.seq Check: 175 from: 1 to: 2916 KIEE2589.orf With 182 enzymes: SgfI * October 20, 2015 16:07 .. Acc65I G'GTAC_C Cuts at: 0 1367 2916 Size: 1367 1549 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1470 1624 1921 2286 2916 Size: 1470 154 297 365 630 Fragments arranged by size: 1470 630 365 297 154 AfeI AGC'GCT Cuts at: 0 2882 2916 Size: 2882 34 AflIII A'CryG_T Cuts at: 0 2462 2916 Size: 2462 454 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 196 2916 Size: 196 2720 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1375 1407 2916 Size: 1375 32 1509 Fragments arranged by size: 1509 1375 32 AlwNI CAG_nnn'CTG Cuts at: 0 84 466 513 618 841 1090 1602 2838 Size: 84 382 47 105 223 249 512 1236 Cuts at: 2838 2916 Size: 78 Fragments arranged by size: 1236 512 382 249 223 105 84 78 47 ApaI G_GGCC'C Cuts at: 0 2082 2594 2916 Size: 2082 512 322 Fragments arranged by size: 2082 512 322 ApaLI G'TGCA_C Cuts at: 0 313 2916 Size: 313 2603 ApoI r'AATT_y Cuts at: 0 451 1771 2916 Size: 451 1320 1145 Fragments arranged by size: 1320 1145 451 AvaI C'yCGr_G Cuts at: 0 427 995 2916 Size: 427 568 1921 Fragments arranged by size: 1921 568 427 BanI G'GyrC_C Cuts at: 0 343 1367 1625 2916 Size: 343 1024 258 1291 Fragments arranged by size: 1291 1024 343 258 BanII G_rGCy'C Cuts at: 0 29 592 886 1257 1898 2082 2594 2609 Size: 29 563 294 371 641 184 512 15 Cuts at: 2609 2717 2873 2916 Size: 108 156 43 Fragments arranged by size: 641 563 512 371 294 184 156 108 43 29 15 BbeI G_GCGC'C Cuts at: 0 1629 2916 Size: 1629 1287 BbsI GAAGACnn'nnnn_ Cuts at: 0 103 1404 1583 2916 Size: 103 1301 179 1333 Fragments arranged by size: 1333 1301 179 103 BbvCI CC'TCA_GC Cuts at: 0 1877 2916 Size: 1877 1039 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1809 1843 2916 Size: 1809 34 1073 Fragments arranged by size: 1809 1073 34 BciVI GTATCCnnnnn_n' Cuts at: 0 1224 2916 Size: 1224 1692 BfrBI ATG'CAT Cuts at: 0 2642 2916 Size: 2642 274 BglI GCCn_nnn'nGGC Cuts at: 0 2266 2916 Size: 2266 650 BglII A'GATC_T Cuts at: 0 733 2348 2916 Size: 733 1615 568 Fragments arranged by size: 1615 733 568 BlpI GC'TnA_GC Cuts at: 0 665 1673 2603 2916 Size: 665 1008 930 313 Fragments arranged by size: 1008 930 665 313 Bme1580I G_kGCm'C Cuts at: 0 317 713 791 2082 2394 2594 2916 Size: 317 396 78 1291 312 200 322 Fragments arranged by size: 1291 396 322 317 312 200 78 BmgBI CAC'GTC Cuts at: 0 792 1871 2916 Size: 792 1079 1045 Fragments arranged by size: 1079 1045 792 BmrI ACTGGGnnnn_n' Cuts at: 0 626 823 1704 1833 2916 Size: 626 197 881 129 1083 Fragments arranged by size: 1083 881 626 197 129 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 870 1050 1230 1241 1683 1963 2860 2916 Size: 870 180 180 11 442 280 897 56 Fragments arranged by size: 897 870 442 280 180 180 56 11 Bpu10I CC'TnA_GC Cuts at: 0 1377 1877 2916 Size: 1377 500 1039 Fragments arranged by size: 1377 1039 500 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 758 1797 2916 Size: 758 1039 1119 Fragments arranged by size: 1119 1039 758 BsaAI yAC'GTr Cuts at: 0 294 1723 2916 Size: 294 1429 1193 Fragments arranged by size: 1429 1193 294 BsaBI GATnn'nnATC Cuts at: 0 2269 2916 Size: 2269 647 BsaHI Gr'CG_yC Cuts at: 0 1626 2916 Size: 1626 1290 BsaWI w'CCGG_w Cuts at: 0 263 2013 2177 2916 Size: 263 1750 164 739 Fragments arranged by size: 1750 739 263 164 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1204 1234 1930 1960 2916 Size: 1204 30 696 30 956 Fragments arranged by size: 1204 956 696 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 55 70 1053 1570 1573 2878 2881 2916 Size: 55 15 983 517 3 1305 3 35 Fragments arranged by size: 1305 983 517 55 35 15 3 3 BseYI C'CCAG_C Cuts at: 0 84 410 1333 2275 2586 2916 Size: 84 326 923 942 311 330 Fragments arranged by size: 942 923 330 326 311 84 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 827 1846 2183 2395 2916 Size: 827 1019 337 212 521 Fragments arranged by size: 1019 827 521 337 212 BsiEI CG_ry'CG Cuts at: 0 7 2916 Size: 7 2909 BsiHKAI G_wGCw'C Cuts at: 0 29 74 317 886 1257 1378 1679 2609 Size: 29 45 243 569 371 121 301 930 Cuts at: 2609 2717 2916 Size: 108 199 Fragments arranged by size: 930 569 371 301 243 199 121 108 45 29 BsmI GAATG_Cn' Cuts at: 0 669 1482 2262 2916 Size: 669 813 780 654 Fragments arranged by size: 813 780 669 654 BsmBI CGTCTCn'nnnn_ Cuts at: 0 798 2916 Size: 798 2118 Bsp1286I G_dGCh'C Cuts at: 0 29 74 317 592 713 791 886 1257 Size: 29 45 243 275 121 78 95 371 Cuts at: 1257 1378 1679 1898 2082 2394 2594 2609 2717 Size: 121 301 219 184 312 200 15 108 Cuts at: 2717 2873 2916 Size: 156 43 Fragments arranged by size: 371 312 301 275 243 219 200 184 156 121 121 108 95 78 45 43 29 15 BspEI T'CCGG_A Cuts at: 0 2177 2916 Size: 2177 739 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1145 1658 2345 2916 Size: 1145 513 687 571 Fragments arranged by size: 1145 687 571 513 BsrBI CCG'CTC Cuts at: 0 165 594 2799 2916 Size: 165 429 2205 117 Fragments arranged by size: 2205 429 165 117 BsrDI GCAATG_nn' Cuts at: 0 1038 1238 1275 2916 Size: 1038 200 37 1641 Fragments arranged by size: 1641 1038 200 37 BsrGI T'GTAC_A Cuts at: 0 1343 2916 Size: 1343 1573 BssSI C'ACGA_G Cuts at: 0 316 583 2251 2916 Size: 316 267 1668 665 Fragments arranged by size: 1668 665 316 267 BstAPI GCAn_nnn'nTGC Cuts at: 0 387 2648 2916 Size: 387 2261 268 Fragments arranged by size: 2261 387 268 BstEII G'GTnAC_C Cuts at: 0 1692 2916 Size: 1692 1224 BstYI r'GATC_y Cuts at: 0 733 1592 1613 2348 2916 Size: 733 859 21 735 568 Fragments arranged by size: 859 735 733 568 21 Bsu36I CC'TnA_GG Cuts at: 0 53 1104 2916 Size: 53 1051 1812 Fragments arranged by size: 1812 1051 53 BtgI C'CryG_G Cuts at: 0 1889 2138 2873 2916 Size: 1889 249 735 43 Fragments arranged by size: 1889 735 249 43 BtsI GCAGTG_nn' Cuts at: 0 339 396 514 527 1323 1851 1999 2916 Size: 339 57 118 13 796 528 148 917 Fragments arranged by size: 917 796 528 339 148 118 57 13 ClaI AT'CG_AT Cuts at: 0 557 2916 Size: 557 2359 DrdI GACnn_nn'nnGTC Cuts at: 0 490 2916 Size: 490 2426 EaeI y'GGCC_r Cuts at: 0 4 497 974 1886 2141 2267 2483 2825 Size: 4 493 477 912 255 126 216 342 Cuts at: 2825 2916 Size: 91 Fragments arranged by size: 912 493 477 342 255 216 126 91 4 EagI C'GGCC_G Cuts at: 0 4 2916 Size: 4 2912 EarI CTCTTCn'nnn_ Cuts at: 0 1104 1565 2371 2732 2916 Size: 1104 461 806 361 184 Fragments arranged by size: 1104 806 461 361 184 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1606 2916 Size: 1606 1310 EcoICRI GAG'CTC Cuts at: 0 27 884 1255 2607 2715 2916 Size: 27 857 371 1352 108 201 Fragments arranged by size: 1352 857 371 201 108 27 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 870 1050 1230 1241 1470 1624 1683 1921 Size: 870 180 180 11 229 154 59 238 Cuts at: 1921 1963 2286 2860 2916 Size: 42 323 574 56 Fragments arranged by size: 870 574 323 238 229 180 180 154 59 56 42 11 EcoNI CCTnn'n_nnAGG Cuts at: 0 52 1468 1555 2530 2916 Size: 52 1416 87 975 386 Fragments arranged by size: 1416 975 386 87 52 EcoO109I rG'GnC_Cy Cuts at: 0 683 961 1291 2079 2334 2535 2590 2591 Size: 683 278 330 788 255 201 55 1 Cuts at: 2591 2916 Size: 325 Fragments arranged by size: 788 683 330 325 278 255 201 55 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 755 2316 2916 Size: 755 1561 600 Fragments arranged by size: 1561 755 600 FspI TGC'GCA Cuts at: 0 2290 2916 Size: 2290 626 HaeII r_GCGC'y Cuts at: 0 48 1629 2884 2916 Size: 48 1581 1255 32 Fragments arranged by size: 1581 1255 48 32 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 428 460 718 750 776 808 1403 1435 Size: 428 32 258 32 26 32 595 32 Cuts at: 1435 1735 1767 2159 2191 2630 2662 2916 Size: 300 32 392 32 439 32 254 Fragments arranged by size: 595 439 428 392 300 258 254 32 32 32 32 32 32 32 26 HindIII A'AGCT_T Cuts at: 0 1683 2916 Size: 1683 1233 KasI G'GCGC_C Cuts at: 0 1625 2916 Size: 1625 1291 KpnI G_GTAC'C Cuts at: 0 1371 2916 Size: 1371 1545 MscI TGG'CCA Cuts at: 0 976 1888 2143 2269 2485 2827 2916 Size: 976 912 255 126 216 342 89 Fragments arranged by size: 976 912 342 255 216 126 89 MslI CAynn'nnrTG Cuts at: 0 310 499 2255 2916 Size: 310 189 1756 661 Fragments arranged by size: 1756 661 310 189 MspA1I CmG'CkG Cuts at: 0 1013 1137 1454 2159 2445 2875 2916 Size: 1013 124 317 705 286 430 41 Fragments arranged by size: 1013 705 430 317 286 124 41 NarI GG'CG_CC Cuts at: 0 1626 2916 Size: 1626 1290 NcoI C'CATG_G Cuts at: 0 1889 2916 Size: 1889 1027 NsiI A_TGCA'T Cuts at: 0 2644 2916 Size: 2644 272 NspI r_CATG'y Cuts at: 0 183 1392 2466 2646 2916 Size: 183 1209 1074 180 270 Fragments arranged by size: 1209 1074 270 183 180 PciI A'CATG_T Cuts at: 0 2462 2916 Size: 2462 454 PflMI CCAn_nnn'nTGG Cuts at: 0 140 754 2916 Size: 140 614 2162 Fragments arranged by size: 2162 614 140 PfoI T'CCnGG_A Cuts at: 0 1595 1955 2903 2916 Size: 1595 360 948 13 Fragments arranged by size: 1595 948 360 13 PmlI CAC'GTG Cuts at: 0 294 1723 2916 Size: 294 1429 1193 Fragments arranged by size: 1429 1193 294 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1032 1064 1795 1827 2916 Size: 1032 32 731 32 1089 Fragments arranged by size: 1089 1032 731 32 32 PpuMI rG'GwC_Cy Cuts at: 0 683 2334 2535 2916 Size: 683 1651 201 381 Fragments arranged by size: 1651 683 381 201 PspOMI G'GGCC_C Cuts at: 0 2078 2590 2916 Size: 2078 512 326 Fragments arranged by size: 2078 512 326 PstI C_TGCA'G Cuts at: 0 380 392 509 2807 2916 Size: 380 12 117 2298 109 Fragments arranged by size: 2298 380 117 109 12 PvuII CAG'CTG Cuts at: 0 1013 1137 1454 2445 2916 Size: 1013 124 317 991 471 Fragments arranged by size: 1013 991 471 317 124 SacI G_AGCT'C Cuts at: 0 29 886 1257 2609 2717 2916 Size: 29 857 371 1352 108 199 Fragments arranged by size: 1352 857 371 199 108 29 SacII CC_GC'GG Cuts at: 0 2876 2916 Size: 2876 40 ScaI AGT'ACT Cuts at: 0 891 2233 2311 2916 Size: 891 1342 78 605 Fragments arranged by size: 1342 891 605 78 SfcI C'TryA_G Cuts at: 0 376 388 505 2397 2803 2916 Size: 376 12 117 1892 406 113 Fragments arranged by size: 1892 406 376 117 113 12 SfoI GGC'GCC Cuts at: 0 1627 2916 Size: 1627 1289 SmlI C'TyrA_G Cuts at: 0 773 1812 2916 Size: 773 1039 1104 Fragments arranged by size: 1104 1039 773 SphI G_CATG'C Cuts at: 0 2646 2916 Size: 2646 270 StyI C'CwwG_G Cuts at: 0 1889 2330 2486 2916 Size: 1889 441 156 430 Fragments arranged by size: 1889 441 430 156 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1347 2916 Size: 1347 1569 TatI w'GTAC_w Cuts at: 0 889 1343 2231 2309 2546 2916 Size: 889 454 888 78 237 370 Fragments arranged by size: 889 888 454 370 237 78 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1204 1236 1930 1962 2916 Size: 1204 32 694 32 954 Fragments arranged by size: 1204 954 694 32 32 Tth111I GACn'n_nGTC Cuts at: 0 2512 2916 Size: 2512 404 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 984 1120 2493 2835 2916 Size: 984 136 1373 342 81 Fragments arranged by size: 1373 984 342 136 81 XmnI GAAnn'nnTTC Cuts at: 0 2464 2916 Size: 2464 452 Enzymes that do cut: Acc65I AcuI AfeI AflIII AlfI AloI AlwNI ApaI ApaLI ApoI AvaI BanI BanII BbeI BbsI BbvCI BcgI BciVI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BsrGI BssSI BstAPI BstEII BstYI Bsu36I BtgI BtsI ClaI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI HaeII Hin4I HindIII KasI KpnI MscI MslI MspA1I NarI NcoI NsiI NspI PciI PflMI PfoI PmlI PpiI PpuMI PspOMI PstI PvuII SacI SacII ScaI SfcI SfoI SmlI SphI StyI TaqII TatI TstI Tth111I XcmI XmnI Enzymes that do not cut: AarI AatII AccI AclI AflII AgeI AhdI AleI AscI AseI AsiSI AvrII BaeI BamHI BclI BmtI BplI BsaI BsiWI BspHI BsrFI BssHII BstBI BstXI BstZ17I BtgZI CspCI DraI DraIII EcoRI EcoRV FseI FspAI HincII HpaI MfeI MluI MmeI NaeI NdeI NgoMIV NheI NotI NruI PacI PasI PmeI PshAI PsiI PspXI PsrI PvuI RsrII SalI SanDI SapI SbfI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI StuI SwaI XbaI XhoI XmaI ZraI