################################## # NO CUT ENZYMES ( 137 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AflII AflIII AgeI AocI ApaI ApoI AscI AseI AsnI Asp700I Asp718I AspI AvrII BalI BamHI BanI BbeI BbrPI BbvCI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaHI BsaI BsaWI BseAI BsgI BsiWI BsmI BspDI BspEI BspHI BspLU11I BsrBI BsrDI BssSI Bst1107I BstBI BstEII BstF5I BstPI BstXI BstYI BstZ17I Bsu36I CelII ClaI CpoI DraI DrdI EarI Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FokI FseI Hin1I HindIII HpaI KasI KpnI Ksp632I MfeI MflI MluI MluNI MroI MscI MseI MslI MunI NarI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SmaI SmlI SnaBI SpeI Sse8387I SspI StuI SwaI TaqI Tru9I Tsp509I Tth111I TthHB8I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AfaI 721 GTAC GT^AC AhdI 583 GACTACGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwI 502 GATCC GGATC(4/5) AlwNI 313 CAGGCGCTG CAG[ACGT][ACGT][ACGT]^CTG AspEI 583 GACTACGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaI 90 CTCGGG C^[CT]CG[GA]G AviII 217 TGCGCA TGC^GCA BcgI 129 CGACGCGCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BsaBI 531 GATAATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseRI 618 CTCCTC GAGGAG(10/8) BsmBI 477 CGTCTC CGTCTC(1/5) BsoBI 90 CTCGGG C^[CT]CG[GA]G Bsp1407I 720 TGTACA T^GTACA BspMI 377 ACCTGC ACCTGC(4/8) BsrGI 720 TGTACA T^GTACA BstAPI 312 GCAGGCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC DpnII 502 GATC ^GATC DraII 102 GGGTCCC [GA]G^G[ACGT]CC[CT] DraIII 490 CACCGGGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 327 CGGCCG [CT]^GGCC[GA] EagI 327 CGGCCG C^GGCCG Eam1105I 583 GACTACGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 327 CGGCCG C^GGCCG Eco52I 327 CGGCCG C^GGCCG EcoO109I 102 GGGTCCC [GA]G^G[ACGT]CC[CT] EcoT14I 848 CCAAGG C^C[AT][AT]GG FspI 217 TGCGCA TGC^GCA MaeIII 737 GTGAC ^GT[ACGT]AC MamI 531 GATAATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MboI 502 GATC ^GATC NaeI 454 GCCGGC GCC^GGC NdeII 502 GATC ^GATC NgoMIV 454 GCCGGC G^CCGGC PflMI 17 CCACGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 102 GGGTCCC [GA]G^G[AT]CC[CT] PstI 310 CTGCAG CTGCA^G RsaI 721 GTAC GT^AC SacI 87 GAGCTC GAGCT^C Sau3AI 502 GATC ^GATC SfaNI 245 GCATC GCATC(5/9) SgrAI 65 CGCCGGTG C[GA]^CCGG[CT]G SspBI 720 TGTACA T^GTACA StyI 848 CCAAGG C^C[AT][AT]GG TfiI 681 GAATC G^A[AT]TC Tsp45I 737 GTGAC ^GT[GC]AC Van91I 17 CCACGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### Alw44I 137 GTGCAC G^TGCAC 865 GTGCAC Aor51HI 257 AGCGCT AGC^GCT 569 AGCGCT ApaLI 137 GTGCAC G^TGCAC 865 GTGCAC AvaII 103 GGTCC G^G[AT]CC 649 GGTCC BbsI 442 GAAGAC GAAGAC(2/6) 670 GAAGAC BcnI 193 CCGGG CC^[GC]GG 492 CCGGG BfaI 350 CTAG C^TAG 428 CTAG BpmI 398 CTCCAG CTGGAG(16/14) 480 CTCCAG BpuAI 442 GAAGAC GAAGAC(2/6) 670 GAAGAC BsiEI 304 CGACCG CG[GA][CT]^CG 327 CGGCCG BsrFI 66 GCCGGT [GA]^CCGG[CT] 454 GCCGGC BsrI 141 ACTGG ACTGG(1/-1) 147 CCAGT BssHII 152 GCGCGC G^CGCGC 369 GCGCGC Cfr10I 66 GCCGGT [GA]^CCGG[CT] 454 GCCGGC Eco47III 257 AGCGCT AGC^GCT 569 AGCGCT EcoNI 775 CCTTCCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 829 CCTCTCCCAGG HgaI 130 GACGC GACGC(5/10) 341 GCGTC HincII 343 GTCAAC GT[CT]^[GA]AC 580 GTTGAC HindII 343 GTCAAC GT[CT]^[GA]AC 580 GTTGAC HphI 403 GGTGA GGTGA(8/7) 684 TCACC KspI 57 CCGCGG CCGC^GG 330 CCGCGG MaeI 350 CTAG C^TAG 428 CTAG MaeII 214 ACGT A^CGT 863 ACGT MboII 442 GAAGA GAAGA(8/7) 670 GAAGA NciI 193 CCGGG CC^[GC]GG 492 CCGGG NlaIII 553 CATG CATG^ 879 CATG NspI 552 GCATGC [GA]CATG^[CT] 878 GCATGC SacII 57 CCGCGG CCGC^GG 330 CCGCGG SfcI 310 CTGCAG C^T[GA][CT]AG 873 CTACAG SphI 552 GCATGC GCATG^C 878 GCATGC ######################################### # 3 OR MORE CUT ENZYMES ( 45 enzymes) # ######################################### AccII AciI AluI AspHI BanII BbvI BmyI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BssKI BstNI BstUI Cac8I CfoI Cfr13I DdeI DsaI EcoRII FauI Fnu4HI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII ItaI MnlI MspA1I MspI MvaI MvnI MwoI NlaIV PleI Sau96I ScrFI TseI TspRI