(Linear) (Six-base) MAPSORT of: KIEE2678.seq Check: 7201 from: 1 to: 4452 KIEE2678.orf With 182 enzymes: SgfI * July 16, 2019 14:21 .. AatII G_ACGT'C Cuts at: 0 3820 4452 Size: 3820 632 AccI GT'mk_AC Cuts at: 0 1568 3446 4452 Size: 1568 1878 1006 Fragments arranged by size: 1878 1568 1006 Acc65I G'GTAC_C Cuts at: 0 192 3576 4452 Size: 192 3384 876 Fragments arranged by size: 3384 876 192 AclI AA'CG_TT Cuts at: 0 2905 4452 Size: 2905 1547 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1035 1214 1722 2859 2944 3286 3351 4200 Size: 1035 179 508 1137 85 342 65 849 Cuts at: 4200 4452 Size: 252 Fragments arranged by size: 1137 1035 849 508 342 252 179 85 65 AfeI AGC'GCT Cuts at: 0 1665 3361 4452 Size: 1665 1696 1091 Fragments arranged by size: 1696 1665 1091 AflII C'TTAA_G Cuts at: 0 1393 4452 Size: 1393 3059 AflIII A'CryG_T Cuts at: 0 617 761 2468 2936 4013 4452 Size: 617 144 1707 468 1077 439 Fragments arranged by size: 1707 1077 617 468 439 144 AgeI A'CCGG_T Cuts at: 0 3623 4452 Size: 3623 829 AhdI GACnn_n'nnGTC Cuts at: 0 921 4452 Size: 921 3531 AleI CACnn'nnGTG Cuts at: 0 3682 3919 4452 Size: 3682 237 533 Fragments arranged by size: 3682 533 237 AlwNI CAG_nnn'CTG Cuts at: 0 808 1192 3786 4452 Size: 808 384 2594 666 Fragments arranged by size: 2594 808 666 384 ApaI G_GGCC'C Cuts at: 0 3515 4269 4452 Size: 3515 754 183 Fragments arranged by size: 3515 754 183 ApoI r'AATT_y Cuts at: 0 1041 1400 3121 4452 Size: 1041 359 1721 1331 Fragments arranged by size: 1721 1331 1041 359 AvaI C'yCGr_G Cuts at: 0 197 3699 4452 Size: 197 3502 753 Fragments arranged by size: 3502 753 197 BanI G'GyrC_C Cuts at: 0 192 1239 1760 3576 4452 Size: 192 1047 521 1816 876 Fragments arranged by size: 1816 1047 876 521 192 BanII G_rGCy'C Cuts at: 0 78 94 388 830 950 1534 3008 3515 Size: 78 16 294 442 120 584 1474 507 Cuts at: 3515 3853 4003 4269 4452 Size: 338 150 266 183 Fragments arranged by size: 1474 584 507 442 338 294 266 183 150 120 78 16 BbsI GAAGACnn'nnnn_ Cuts at: 0 2454 2574 4452 Size: 2454 120 1878 Fragments arranged by size: 2454 1878 120 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 3208 3242 4452 Size: 3208 34 1210 Fragments arranged by size: 3208 1210 34 BciVI GTATCCnnnnn_n' Cuts at: 0 786 4452 Size: 786 3666 BclI T'GATC_A Cuts at: 0 1016 4452 Size: 1016 3436 BfrBI ATG'CAT Cuts at: 0 2106 2149 4452 Size: 2106 43 2303 Fragments arranged by size: 2303 2106 43 BglI GCCn_nnn'nGGC Cuts at: 0 55 1522 3808 3949 4452 Size: 55 1467 2286 141 503 Fragments arranged by size: 2286 1467 503 141 55 BlpI GC'TnA_GC Cuts at: 0 4117 4452 Size: 4117 335 Bme1580I G_kGCm'C Cuts at: 0 130 776 1583 2060 3515 4070 4269 4452 Size: 130 646 807 477 1455 555 199 183 Fragments arranged by size: 1455 807 646 555 477 199 183 130 BmrI ACTGGGnnnn_n' Cuts at: 0 770 833 3033 3444 3799 4452 Size: 770 63 2200 411 355 653 Fragments arranged by size: 2200 770 653 411 355 63 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 3789 4452 Size: 3789 663 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1252 4386 4452 Size: 1252 3134 66 Fragments arranged by size: 3134 1252 66 Bpu10I CC'TnA_GC Cuts at: 0 944 1996 4452 Size: 944 1052 2456 Fragments arranged by size: 2456 1052 944 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 643 4452 Size: 643 3809 BsaI GGTCTCn'nnnn_ Cuts at: 0 217 2010 3533 4296 4452 Size: 217 1793 1523 763 156 Fragments arranged by size: 1793 1523 763 217 156 BsaAI yAC'GTr Cuts at: 0 764 3444 4452 Size: 764 2680 1008 Fragments arranged by size: 2680 1008 764 BsaBI GATnn'nnATC Cuts at: 0 1211 4452 Size: 1211 3241 BsaHI Gr'CG_yC Cuts at: 0 3817 4240 4452 Size: 3817 423 212 Fragments arranged by size: 3817 423 212 BsaWI w'CCGG_w Cuts at: 0 272 3623 3719 3881 4452 Size: 272 3351 96 162 571 Fragments arranged by size: 3351 571 272 162 96 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 169 199 370 400 1099 1129 1487 1517 Size: 169 30 171 30 699 30 358 30 Cuts at: 1517 2626 2656 2668 2698 3610 3640 3658 3688 Size: 1109 30 12 30 912 30 18 30 Cuts at: 3688 4452 Size: 764 Fragments arranged by size: 1109 912 764 699 358 171 169 30 30 30 30 30 30 30 30 18 12 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 378 613 1249 1357 2647 2878 3075 3521 Size: 378 235 636 108 1290 231 197 446 Cuts at: 3521 4452 Size: 931 Fragments arranged by size: 1290 931 636 446 378 235 231 197 108 BseYI C'CCAG_C Cuts at: 0 97 587 4452 Size: 97 490 3865 Fragments arranged by size: 3865 490 97 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1879 3566 3649 4452 Size: 1879 1687 83 803 Fragments arranged by size: 1879 1687 803 83 BsiEI CG_ry'CG Cuts at: 0 1558 2630 3141 3337 4452 Size: 1558 1072 511 196 1115 Fragments arranged by size: 1558 1115 1072 511 196 BsiHKAI G_wGCw'C Cuts at: 0 78 932 2810 3443 4042 4452 Size: 78 854 1878 633 599 410 Fragments arranged by size: 1878 854 633 599 410 78 BsmI GAATG_Cn' Cuts at: 0 2826 4452 Size: 2826 1626 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1710 4452 Size: 1710 2742 Bsp1286I G_dGCh'C Cuts at: 0 78 94 130 388 776 830 932 950 Size: 78 16 36 258 388 54 102 18 Cuts at: 950 1534 1583 2060 2810 3008 3443 3515 3853 Size: 584 49 477 750 198 435 72 338 Cuts at: 3853 4003 4042 4070 4269 4452 Size: 150 39 28 199 183 Fragments arranged by size: 750 584 477 435 388 338 258 199 198 183 150 102 78 72 54 49 39 36 28 18 16 BspHI T'CATG_A Cuts at: 0 1505 1958 2423 4452 Size: 1505 453 465 2029 Fragments arranged by size: 2029 1505 465 453 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1291 2924 3588 3935 4452 Size: 1291 1633 664 347 517 Fragments arranged by size: 1633 1291 664 517 347 BsrBI CCG'CTC Cuts at: 0 13 3170 3344 3471 4452 Size: 13 3157 174 127 981 Fragments arranged by size: 3157 981 174 127 13 BsrDI GCAATG_nn' Cuts at: 0 107 1629 2149 2288 2541 3231 4413 4452 Size: 107 1522 520 139 253 690 1182 39 Fragments arranged by size: 1522 1182 690 520 253 139 107 39 BsrFI r'CCGG_y Cuts at: 0 2966 3623 3795 3896 4284 4452 Size: 2966 657 172 101 388 168 Fragments arranged by size: 2966 657 388 172 168 101 BssSI C'ACGA_G Cuts at: 0 121 160 973 4452 Size: 121 39 813 3479 Fragments arranged by size: 3479 813 121 39 BstAPI GCAn_nnn'nTGC Cuts at: 0 3641 4452 Size: 3641 811 BstEII G'GTnAC_C Cuts at: 0 1440 2244 4452 Size: 1440 804 2208 Fragments arranged by size: 2208 1440 804 BstXI CCAn_nnnn'nTGG Cuts at: 0 783 1890 1990 2721 2980 4452 Size: 783 1107 100 731 259 1472 Fragments arranged by size: 1472 1107 783 731 259 100 BstYI r'GATC_y Cuts at: 0 278 329 554 644 4452 Size: 278 51 225 90 3808 Fragments arranged by size: 3808 278 225 90 51 BtgI C'CryG_G Cuts at: 0 188 378 1712 2483 2616 4194 4226 4452 Size: 188 190 1334 771 133 1578 32 226 Fragments arranged by size: 1578 1334 771 226 190 188 133 32 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 306 4452 Size: 306 4146 BtsI GCAGTG_nn' Cuts at: 0 902 1532 1937 2207 2262 2660 2991 4070 Size: 902 630 405 270 55 398 331 1079 Cuts at: 4070 4452 Size: 382 Fragments arranged by size: 1079 902 630 405 398 382 331 270 55 ClaI AT'CG_AT Cuts at: 0 2999 4452 Size: 2999 1453 DraIII CAC_nnn'GTG Cuts at: 0 3564 4452 Size: 3564 888 EaeI y'GGCC_r Cuts at: 0 47 56 143 728 1928 2480 2711 3150 Size: 47 9 87 585 1200 552 231 439 Cuts at: 3150 3284 4452 Size: 134 1168 Fragments arranged by size: 1200 1168 585 552 439 231 134 87 47 9 EarI CTCTTCn'nnn_ Cuts at: 0 82 973 1190 1504 2878 4452 Size: 82 891 217 314 1374 1574 Fragments arranged by size: 1574 1374 891 314 217 82 EcoICRI GAG'CTC Cuts at: 0 76 4452 Size: 76 4376 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1035 1214 1252 1722 2859 2944 3286 3351 Size: 1035 179 38 470 1137 85 342 65 Cuts at: 3351 4200 4386 4452 Size: 849 186 66 Fragments arranged by size: 1137 1035 849 470 342 186 179 85 66 65 38 EcoNI CCTnn'n_nnAGG Cuts at: 0 1234 1287 3931 4452 Size: 1234 53 2644 521 Fragments arranged by size: 2644 1234 521 53 EcoO109I rG'GnC_Cy Cuts at: 0 603 1514 3511 3512 4266 4452 Size: 603 911 1997 1 754 186 Fragments arranged by size: 1997 911 754 603 186 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 3381 4452 Size: 3381 1071 FspI TGC'GCA Cuts at: 0 2707 4452 Size: 2707 1745 HaeII r_GCGC'y Cuts at: 0 1667 2322 2640 2762 3363 4452 Size: 1667 655 318 122 601 1089 Fragments arranged by size: 1667 1089 655 601 318 122 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 250 282 403 435 636 668 907 939 Size: 250 32 121 32 201 32 239 32 Cuts at: 939 1487 1519 1540 1572 2626 2658 2824 2856 Size: 548 32 21 32 1054 32 166 32 Cuts at: 2856 3610 3642 4452 Size: 754 32 810 Fragments arranged by size: 1054 810 754 548 250 239 201 166 121 32 32 32 32 32 32 32 32 32 21 HincII GTy'rAC Cuts at: 0 1569 4452 Size: 1569 2883 HindIII A'AGCT_T Cuts at: 0 458 4452 Size: 458 3994 KpnI G_GTAC'C Cuts at: 0 196 3580 4452 Size: 196 3384 872 Fragments arranged by size: 3384 872 196 MfeI C'AATT_G Cuts at: 0 446 4452 Size: 446 4006 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 158 2562 2711 2799 4379 4452 Size: 158 2404 149 88 1580 73 Fragments arranged by size: 2404 1580 158 149 88 73 MscI TGG'CCA Cuts at: 0 145 730 1930 3152 4452 Size: 145 585 1200 1222 1300 Fragments arranged by size: 1300 1222 1200 585 145 MslI CAynn'nnrTG Cuts at: 0 121 1525 3682 3919 4418 4452 Size: 121 1404 2157 237 499 34 Fragments arranged by size: 2157 1404 499 237 121 34 MspA1I CmG'CkG Cuts at: 0 2436 2609 2922 3503 3572 3650 4030 4452 Size: 2436 173 313 581 69 78 380 422 Fragments arranged by size: 2436 581 422 380 313 173 78 69 NaeI GCC'GGC Cuts at: 0 3797 3898 4452 Size: 3797 101 554 Fragments arranged by size: 3797 554 101 NcoI C'CATG_G Cuts at: 0 2483 2616 4194 4452 Size: 2483 133 1578 258 Fragments arranged by size: 2483 1578 258 133 NdeI CA'TA_TG Cuts at: 0 843 4452 Size: 843 3609 NgoMIV G'CCGG_C Cuts at: 0 3795 3896 4452 Size: 3795 101 556 Fragments arranged by size: 3795 556 101 NsiI A_TGCA'T Cuts at: 0 2108 2151 4452 Size: 2108 43 2301 Fragments arranged by size: 2301 2108 43 NspI r_CATG'y Cuts at: 0 621 921 2110 2472 4017 4116 4417 4452 Size: 621 300 1189 362 1545 99 301 35 Fragments arranged by size: 1545 1189 621 362 301 300 99 35 PasI CC'CwG_GG Cuts at: 0 4385 4452 Size: 4385 67 PciI A'CATG_T Cuts at: 0 617 2468 4013 4452 Size: 617 1851 1545 439 Fragments arranged by size: 1851 1545 617 439 PflMI CCAn_nnn'nTGG Cuts at: 0 35 288 2622 4452 Size: 35 253 2334 1830 Fragments arranged by size: 2334 1830 253 35 PmlI CAC'GTG Cuts at: 0 764 4452 Size: 764 3688 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2890 2922 4452 Size: 2890 32 1530 Fragments arranged by size: 2890 1530 32 PpuMI rG'GwC_Cy Cuts at: 0 603 4452 Size: 603 3849 PshAI GACnn'nnGTC Cuts at: 0 2781 4452 Size: 2781 1671 PsiI TTA'TAA Cuts at: 0 1754 4452 Size: 1754 2698 PspOMI G'GGCC_C Cuts at: 0 3511 4265 4452 Size: 3511 754 187 Fragments arranged by size: 3511 754 187 PstI C_TGCA'G Cuts at: 0 1154 2345 2921 3782 3932 4452 Size: 1154 1191 576 861 150 520 Fragments arranged by size: 1191 1154 861 576 520 150 PvuI CG_AT'CG Cuts at: 0 1558 2630 4452 Size: 1558 1072 1822 Fragments arranged by size: 1822 1558 1072 PvuII CAG'CTG Cuts at: 0 2436 2609 2922 3650 4030 4452 Size: 2436 173 313 728 380 422 Fragments arranged by size: 2436 728 422 380 313 173 RsrII CG'GwC_CG Cuts at: 0 3626 4452 Size: 3626 826 SacI G_AGCT'C Cuts at: 0 78 4452 Size: 78 4374 SalI G'TCGA_C Cuts at: 0 1567 4452 Size: 1567 2885 SapI GCTCTTCn'nnn_ Cuts at: 0 82 1190 4452 Size: 82 1108 3262 Fragments arranged by size: 3262 1108 82 SbfI CC_TGCA'GG Cuts at: 0 3782 4452 Size: 3782 670 ScaI AGT'ACT Cuts at: 0 1162 1735 4452 Size: 1162 573 2717 Fragments arranged by size: 2717 1162 573 SexAI A'CCwGG_T Cuts at: 0 1436 1982 4452 Size: 1436 546 2470 Fragments arranged by size: 2470 1436 546 SfcI C'TryA_G Cuts at: 0 1150 2341 2604 2917 3144 3778 3928 4452 Size: 1150 1191 263 313 227 634 150 524 Fragments arranged by size: 1191 1150 634 524 313 263 227 150 SfiI GGCCn_nnn'nGGCC Cuts at: 0 55 4452 Size: 55 4397 SmaI CCC'GGG Cuts at: 0 199 4452 Size: 199 4253 SmlI C'TyrA_G Cuts at: 0 658 1393 4452 Size: 658 735 3059 Fragments arranged by size: 3059 735 658 SphI G_CATG'C Cuts at: 0 2110 4452 Size: 2110 2342 SspI AAT'ATT Cuts at: 0 2082 4452 Size: 2082 2370 StyI C'CwwG_G Cuts at: 0 698 2483 2616 3586 3863 4194 4452 Size: 698 1785 133 970 277 331 258 Fragments arranged by size: 1785 970 698 331 277 258 133 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 241 985 1665 2300 2542 3052 3469 4452 Size: 241 744 680 635 242 510 417 983 Fragments arranged by size: 983 744 680 635 510 417 242 241 TatI w'GTAC_w Cuts at: 0 1160 1733 4452 Size: 1160 573 2719 Fragments arranged by size: 2719 1160 573 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 169 201 370 402 1099 1131 2024 2056 Size: 169 32 169 32 697 32 893 32 Cuts at: 2056 3658 3690 4168 4200 4452 Size: 1602 32 478 32 252 Fragments arranged by size: 1602 893 697 478 252 169 169 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 0 3496 4141 4452 Size: 3496 645 311 Fragments arranged by size: 3496 645 311 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 36 2305 4284 4452 Size: 36 2269 1979 168 Fragments arranged by size: 2269 1979 168 36 XmaI C'CCGG_G Cuts at: 0 197 4452 Size: 197 4255 XmnI GAAnn'nnTTC Cuts at: 0 2872 4452 Size: 2872 1580 ZraI GAC'GTC Cuts at: 0 3818 4452 Size: 3818 634 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlwNI ApaI ApoI AvaI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI DraIII EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI HaeII Hin4I HincII HindIII KpnI MfeI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NsiI NspI PasI PciI PflMI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SexAI SfcI SfiI SmaI SmlI SphI SspI StyI TaqII TatI TstI Tth111I XcmI XmaI XmnI ZraI Enzymes that do not cut: AarI AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BglII BmgBI BmtI BsiWI BspEI BsrGI BssHII BstBI BstZ17I Bsu36I CspCI DraI DrdI EagI EciI EcoRI EcoRV FseI FspAI HpaI KasI MluI NarI NheI NotI NruI PacI PfoI PmeI PspXI PsrI SacII SanDI SfoI SgfI SgrAI SnaBI SpeI SrfI StuI SwaI XbaI XhoI