Restriction map for
pF1KE2794
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# NO CUT ENZYMES ( 90 enzymes) #
##################################
Acc65I AccI AclI AfaI AflII
AflIII AgeI Alw44I Aor51HI ApaLI
AscI AseI AsnI Asp718I BamHI
BbeI BbrPI BclI BfrI BsaAI
BsaBI BsiWI BsmI Bsp1407I BspDI
BspHI BspLU11I BspMI BsrDI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I ClaI CpoI DraI DraIII
DrdI Eco47III EcoNI EcoO65I EcoRI
EcoT22I FbaI FseI HincII HindII
HpaI HphI KasI KpnI MamI
MfeI MluI MunI NarI NdeI
NheI NotI NruI NsiI NspV
PacI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RcaI RsaI
RsrII SacI SalI ScaI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SspBI SspI StuI SwaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AatII 1720 GACGTC GACGT^C
AccIII 1970 TCCGGA T^CCGGA
AcsI 1540 AAATTC [GA]^AATT[CT]
AhdI 1207 GACCGACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 1011 GATCC GGATC(4/5)
ApoI 1540 AAATTC [GA]^AATT[CT]
Asp700I 1536 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1207 GACCGACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 298 GAGCAC G[AT]GC[AT]^C
AspI 730 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 786 CCTAGG C^CTAGG
BalI 1439 TGGCCA TGG^CCA
BbsI 1005 GAAGAC GAAGAC(2/6)
BlnI 786 CCTAGG C^CTAGG
BlpI 1617 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1617 GCTGAGC GC^T[ACGT]AGC
BpuAI 1005 GAAGAC GAAGAC(2/6)
BseAI 1970 TCCGGA T^CCGGA
BsiHKAI 298 GAGCAC G[AT]GC[AT]^C
BsmBI 80 GAGACG CGTCTC(1/5)
BspEI 1970 TCCGGA T^CCGGA
BssSI 1390 CTCGTG CACGAG(-5/-1)
CelII 1617 GCTGAGC GC^T[ACGT]AGC
EagI 375 CGGCCG C^GGCCG
Eam1105I 1207 GACCGACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 375 CGGCCG C^GGCCG
Eco52I 375 CGGCCG C^GGCCG
EcoRV 1075 GATATC GAT^ATC
HindIII 1886 AAGCTT A^AGCTT
KspI 812 CCGCGG CCGC^GG
MluNI 1439 TGGCCA TGG^CCA
MroI 1970 TCCGGA T^CCGGA
MscI 1439 TGGCCA TGG^CCA
MseI 1853 TTAA T^TAA
MslI 335 CACCCCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 599 GCCGGC GCC^GGC
NcoI 1822 CCATGG C^CATGG
NgoMIV 599 GCCGGC G^CCGGC
PflFI 730 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1503 CCAGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 1462 GACCAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 812 CCGCGG CCGC^GG
SapI 535 GAAGAGC GCTCTTC(1/4)
SbfI 657 CCTGCAGG CCTGCA^GG
SphI 639 GCATGC GCATG^C
Sse8387I 657 CCTGCAGG CCTGCA^GG
Tru9I 1853 TTAA T^TAA
Tth111I 730 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1503 CCAGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 912 TCTAGA T^CTAGA
XmnI 1536 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 32 enzymes) #
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AocI 141 CCTAAGG CC^T[ACGT]AGG
241 CCTCAGG
ApaI 237 GGGCCC GGGCC^C
1400 GGGCCC
AviII 1151 TGCGCA TGC^GCA
1253 TGCGCA
BbvCI 165 GCTGAGG CCTCAGC(-5/-2)
1065 GCTGAGG
BcgI 984 CGAGCTGCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
219 GCAGATCTCTCG
BciVI 451 GGATAC GTATCC(6/5)
1789 GTATCC
BglI 761 GCCACTTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1235 GCCCCTCGGGC
BglII 221 AGATCT A^GATCT
1407 AGATCT
BseRI 68 GAGGAG GAGGAG(10/8)
1569 GAGGAG
BsgI 1794 CTGCAC GTGCAG(16/14)
1138 GTGCAG
BsiEI 251 CGACCG CG[GA][CT]^CG
375 CGGCCG
BsrBI 1087 CCGCTC CCGCTC(-3/-3)
1327 GAGCGG
BsrFI 599 GCCGGC [GA]^CCGG[CT]
746 ACCGGC
BstAPI 634 GCACAGCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1140 GCAGACCCTGC
BstXI 334 CCACCCCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
423 CCACTGCCTTGG
BstYI 221 AGATCT [GA]^GATC[CT]
1407 AGATCT
Bsu36I 141 CCTAAGG CC^T[ACGT]AGG
241 CCTCAGG
Cfr10I 599 GCCGGC [GA]^CCGG[CT]
746 ACCGGC
EarI 65 GAAGAG CTCTTC(1/4)
535 GAAGAG
Eco81I 141 CCTAAGG CC^T[ACGT]AGG
241 CCTCAGG
FspI 1151 TGCGCA TGC^GCA
1253 TGCGCA
HaeII 1296 GGCGCT [GA]GCGC^[CT]
1340 GGCGCT
Ksp632I 65 GAAGAG CTCTTC(1/4)
535 GAAGAG
MaeIII 781 GTGAC ^GT[ACGT]AC
1490 GTGAC
MflI 221 AGATCT [GA]^GATC[CT]
1407 AGATCT
NspI 639 GCATGC [GA]CATG^[CT]
1309 ACATGC
PpuMI 306 AGGACCC [GA]G^G[AT]CC[CT]
1817 AGGTCCC
TfiI 511 GAATC G^A[AT]TC
1990 GATTC
Tsp45I 781 GTGAC ^GT[GC]AC
1490 GTGAC
Tsp509I 1541 AATT ^AATT
1760 AATT
XcmI 756 CCAAGGCCACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
852 CCAGACCCTTCCTGG
XhoII 221 AGATCT [GA]^GATC[CT]
1407 AGATCT
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# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AccII AciI AcyI AluI AlwNI
AvaI AvaII BanI BanII BbvI
BcnI BfaI BmyI BpmI BsaHI
BsaI BsaJI BsaWI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII ItaI MaeI MaeII MboI
MboII MnlI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PaeR7I PleI PstI PvuII
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI StyI TaqI TseI
TspRI TthHB8I XhoI XmaCI XmaI