################################## # NO CUT ENZYMES ( 74 enzymes) # ################################## Acc65I AccI AclI AflII Aor51HI AscI AseI AsnI Asp718I AspI AvrII BciVI BclI BfrI BglII BlnI BsiWI BsmI Bsp1407I BspDI BspLU11I BsrDI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI DraI DraIII Eco47III EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HpaI KpnI MfeI MluI MseI MunI NheI NotI NsiI NspV PacI PflFI PmeI PshAI PshBI Psp1406I PvuI RsrII SalI SbfI SexAI SfuI SmaI SnaBI SpeI Sse8387I SspBI SspI SwaI Tru9I Tth111I XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AatII 2265 GACGTC GACGT^C AccIII 815 TCCGGA T^CCGGA AflIII 878 ACGTGT A^C[GA][CT]GT AgeI 1886 ACCGGT A^CCGGT AhdI 2310 GACGCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspEI 2310 GACGCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BbrPI 1786 CACGTG CAC^GTG BbvCI 558 CCTCAGC CCTCAGC(-5/-2) BfaI 2617 CTAG C^TAG BlpI 1834 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1834 GCTGAGC GC^T[ACGT]AGC BsaAI 1786 CACGTG [CT]AC^GT[GA] BsaBI 2805 GATTTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseAI 815 TCCGGA T^CCGGA BspEI 815 TCCGGA T^CCGGA CelII 1834 GCTGAGC GC^T[ACGT]AGC EagI 695 CGGCCG C^GGCCG Eam1105I 2310 GACGCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 695 CGGCCG C^GGCCG Eco52I 695 CGGCCG C^GGCCG HincII 327 GTTGAC GT[CT]^[GA]AC HindII 327 GTTGAC GT[CT]^[GA]AC HindIII 2380 AAGCTT A^AGCTT KspI 503 CCGCGG CCGC^GG MaeI 2617 CTAG C^TAG MamI 2805 GATTTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 815 TCCGGA T^CCGGA NcoI 2426 CCATGG C^CATGG NdeI 848 CATATG CA^TATG PinAI 1886 ACCGGT A^CCGGT PmaCI 1786 CACGTG CAC^GTG PmlI 1786 CACGTG CAC^GTG SacI 2620 GAGCTC GAGCT^C SacII 503 CCGCGG CCGC^GG ScaI 911 AGTACT AGT^ACT SfiI 592 GGCCCTGGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SgrAI 214 CGCCGGCG C[GA]^CCGG[CT]G SphI 2522 GCATGC GCATG^C ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AcsI 1011 AAATTT [GA]^AATT[CT] 1984 AAATTC ApoI 1011 AAATTT [GA]^AATT[CT] 1984 AAATTC Asp700I 1131 GAAGGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2967 GAACGACTTC BalI 1175 TGGCCA TGG^CCA 2066 TGGCCA BcgI 946 CGAAGAAAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 2396 GCATCCGCATCG BsaWI 815 TCCGGA [AT]^CCGG[AT] 1886 ACCGGT BsmBI 3023 GAGACG CGTCTC(1/5) 3340 GAGACG BspHI 1442 TCATGA T^CATGA 3218 TCATGA BssSI 1957 CACGAG CACGAG(-5/-1) 1188 CTCGTG DrdI 873 GACCAACGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1933 GACGCCCACGTC EcoNI 1361 CCTTCAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1548 CCTGAGCCAGG MluNI 1175 TGGCCA TGG^CCA 2066 TGGCCA MscI 1175 TGGCCA TGG^CCA 2066 TGGCCA NaeI 215 GCCGGC GCC^GGC 625 GCCGGC NgoMIV 215 GCCGGC G^CCGGC 625 GCCGGC NruI 725 TCGCGA TCG^CGA 2319 TCGCGA NspI 2522 GCATGC [GA]CATG^[CT] 2686 ACATGC PaeR7I 1964 CTCGAG C^TCGAG 2242 CTCGAG PstI 1234 CTGCAG CTGCA^G 2281 CTGCAG RcaI 1442 TCATGA T^CATGA 3218 TCATGA SapI 605 GAAGAGC GCTCTTC(1/4) 2941 GAAGAGC TfiI 1039 GAATC G^A[AT]TC 1136 GATTC Tsp509I 1012 AATT ^AATT 1985 AATT XhoI 1964 CTCGAG C^TCGAG 2242 CTCGAG XmnI 1131 GAAGGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2967 GAACGACTTC ######################################### # 3 OR MORE CUT ENZYMES ( 121 enzymes) # ######################################### AccII AciI AcyI AfaI AluI Alw44I AlwI AlwNI AocI ApaI ApaLI AspHI AvaI AvaII AviII BamHI BanI BanII BbeI BbsI BbvI BcnI BglI BmyI BpmI BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI FspI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I MaeII MaeIII MboI MboII MflI MnlI MslI MspA1I MspI MvaI MvnI MwoI NarI NciI NdeII NlaIII NlaIV PflMI PleI PpuMI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmlI StuI StyI TaqI TseI Tsp45I TspRI TthHB8I Van91I XcmI XhoII