(Circular) (Six-base) MAPSORT of: pF1KE3374.seq Check: 1994 from: 1 to: 6464 pF1KE3374 6464 bp With 182 enzymes: SgfI * October 24, 2019 20:49 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 611 2382 2457 611 Size: 1771 75 4618 Fragments arranged by size: 4618 1771 75 AatII G_ACGT'C Cuts at: 2377 2377 Size: 6464 AccI GT'mk_AC Cuts at: 3502 3502 Size: 6464 Acc65I G'GTAC_C Cuts at: 3480 3480 Size: 6464 AclI AA'CG_TT Cuts at: 6304 6304 Size: 6464 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1060 1801 2010 4289 4721 5468 5736 1060 Size: 741 209 2279 432 747 268 1788 Fragments arranged by size: 2279 1788 747 741 432 268 209 AflIII A'CryG_T Cuts at: 986 3816 4920 5897 986 Size: 2830 1104 977 1553 Fragments arranged by size: 2830 1553 1104 977 AgeI A'CCGG_T Cuts at: 1994 4852 1994 Size: 2858 3606 AhdI GACnn_n'nnGTC Cuts at: 2423 2423 Size: 6464 AleI CACnn'nnGTG Cuts at: 754 2536 2701 754 Size: 1782 165 4517 Fragments arranged by size: 4517 1782 165 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 760 2533 3044 3169 760 Size: 1773 511 125 4055 Fragments arranged by size: 4055 1773 511 125 AlwNI CAG_nnn'CTG Cuts at: 154 417 561 867 1966 2232 2273 2805 3099 Size: 263 144 306 1099 266 41 532 294 Cuts at: 3099 3769 5336 154 Size: 670 1567 1282 Fragments arranged by size: 1567 1282 1099 670 532 306 294 266 263 144 41 ApaI G_GGCC'C Cuts at: 309 411 897 1673 309 Size: 102 486 776 5100 Fragments arranged by size: 5100 776 486 102 ApaLI G'TGCA_C Cuts at: 422 1084 1702 5234 422 Size: 662 618 3532 1652 Fragments arranged by size: 3532 1652 662 618 ApoI r'AATT_y Cuts at: 1119 2092 3468 5817 6073 1119 Size: 973 1376 2349 256 1510 Fragments arranged by size: 2349 1510 1376 973 256 AseI AT'TA_AT Cuts at: 20 20 Size: 6464 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 6464 AvaI C'yCGr_G Cuts at: 545 1954 2072 2350 3484 545 Size: 1409 118 278 1134 3525 Fragments arranged by size: 3525 1409 1134 278 118 BamHI G'GATC_C Cuts at: 1406 1715 1751 3489 1406 Size: 309 36 1738 4381 Fragments arranged by size: 4381 1738 309 36 BanI G'GyrC_C Cuts at: 144 254 517 550 662 724 736 776 1187 Size: 110 263 33 112 62 12 40 411 Cuts at: 1187 1984 2281 2592 3480 3894 4142 4177 144 Size: 797 297 311 888 414 248 35 2431 Fragments arranged by size: 2431 888 797 414 411 311 297 263 248 112 110 62 40 35 33 12 BanII G_rGCy'C Cuts at: 309 411 897 1673 1949 2327 2483 2732 2768 Size: 102 486 776 276 378 156 249 36 Cuts at: 2768 2871 3478 4508 309 Size: 103 607 1030 2265 Fragments arranged by size: 2265 1030 776 607 486 378 276 249 156 103 102 36 BbeI G_GCGC'C Cuts at: 258 554 666 780 1988 3898 4146 258 Size: 296 112 114 1208 1910 248 2576 Fragments arranged by size: 2576 1910 1208 296 248 114 112 BbsI GAAGACnn'nnnn_ Cuts at: 1641 2004 3337 1641 Size: 363 1333 4768 Fragments arranged by size: 4768 1333 363 BbvCI CC'TCA_GC Cuts at: 667 667 Size: 6464 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1043 1077 2493 2527 1043 Size: 34 1416 34 4980 Fragments arranged by size: 4980 1416 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4362 5123 6464 4362 Size: 761 1341 4362 Fragments arranged by size: 4362 1341 761 BclI T'GATC_A Cuts at: 3984 3984 Size: 6464 BglI GCCn_nnn'nGGC Cuts at: 650 707 1217 2331 650 Size: 57 510 1114 4783 Fragments arranged by size: 4783 1114 510 57 BglII A'GATC_T Cuts at: 3979 6016 3979 Size: 2037 4427 BlpI GC'TnA_GC Cuts at: 1943 3576 1943 Size: 1633 4831 Bme1580I G_kGCm'C Cuts at: 309 344 411 426 897 1088 1673 1706 2284 Size: 35 67 15 471 191 585 33 578 Cuts at: 2284 2698 2742 4089 4182 5238 309 Size: 414 44 1347 93 1056 1535 Fragments arranged by size: 1535 1347 1056 585 578 471 414 191 93 67 44 35 33 15 BmgBI CAC'GTC Cuts at: 2049 2049 Size: 6464 BmrI ACTGGGnnnn_n' Cuts at: 1203 1857 2235 3676 3757 4092 6236 1203 Size: 654 378 1441 81 335 2144 1431 Fragments arranged by size: 2144 1441 1431 654 378 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 1380 4831 1380 Size: 3451 3013 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 176 246 259 315 840 844 960 1129 1140 Size: 70 13 56 525 4 116 169 11 Cuts at: 1140 1533 1705 2077 2740 2798 3076 3424 176 Size: 393 172 372 663 58 278 348 3216 Fragments arranged by size: 3216 663 525 393 372 348 278 172 169 116 70 58 56 13 11 4 Bpu10I CC'TnA_GC Cuts at: 667 1657 2930 3377 667 Size: 990 1273 447 3754 Fragments arranged by size: 3754 1273 990 447 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2656 3026 3640 4519 5011 5309 2656 Size: 370 614 879 492 298 3811 Fragments arranged by size: 3811 879 614 492 370 298 BsaI GGTCTCn'nnnn_ Cuts at: 973 1509 3244 6168 973 Size: 536 1735 2924 1269 Fragments arranged by size: 2924 1735 1269 536 BsaAI yAC'GTr Cuts at: 1896 3817 1896 Size: 1921 4543 BsaBI GATnn'nnATC Cuts at: 6 2917 3978 6015 6 Size: 2911 1061 2037 455 Fragments arranged by size: 2911 2037 1061 455 BsaHI Gr'CG_yC Cuts at: 210 255 551 663 777 1209 1985 2042 2374 Size: 45 296 112 114 432 776 57 332 Cuts at: 2374 2831 3895 4143 4845 6341 210 Size: 457 1064 248 702 1496 333 Fragments arranged by size: 1496 1064 776 702 457 432 333 332 296 248 114 112 57 45 BsaWI w'CCGG_w Cuts at: 923 1994 3653 4174 4852 5126 5273 5684 923 Size: 1071 1659 521 678 274 147 411 1703 Fragments arranged by size: 1703 1659 1071 678 521 411 274 147 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 225 228 475 478 585 1369 1516 1915 225 Size: 3 247 3 107 784 147 399 4774 Fragments arranged by size: 4774 784 399 247 147 107 3 3 BseYI C'CCAG_C Cuts at: 175 223 269 410 554 1368 1928 2327 2691 Size: 48 46 141 144 814 560 399 364 Cuts at: 2691 2937 3109 3889 4647 5224 5720 175 Size: 246 172 780 758 577 496 919 Fragments arranged by size: 919 814 780 758 577 560 496 399 364 246 172 144 141 48 46 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 631 921 2485 2557 2560 2635 3017 3085 3139 Size: 290 1564 72 3 75 382 68 54 Cuts at: 3139 631 Size: 3956 Fragments arranged by size: 3956 1564 382 290 75 72 68 54 3 BsiEI CG_ry'CG Cuts at: 104 806 1995 2865 4006 4052 5260 104 Size: 702 1189 870 1141 46 1208 1308 Fragments arranged by size: 1308 1208 1189 1141 870 702 46 BsiHKAI G_wGCw'C Cuts at: 383 426 968 1088 1366 1706 2312 2441 2732 Size: 43 542 120 278 340 606 129 291 Cuts at: 2732 2879 3478 4256 4446 5238 383 Size: 147 599 778 190 792 1609 Fragments arranged by size: 1609 792 778 606 599 542 340 291 278 190 147 129 120 43 BsmBI CGTCTCn'nnnn_ Cuts at: 3125 3442 3125 Size: 317 6147 Bsp1286I G_dGCh'C Cuts at: 309 344 383 411 426 897 968 1088 1366 Size: 35 39 28 15 471 71 120 278 Cuts at: 1366 1673 1706 1949 2284 2312 2327 2441 2483 Size: 307 33 243 335 28 15 114 42 Cuts at: 2483 2698 2732 2742 2768 2871 2879 3478 4089 Size: 215 34 10 26 103 8 599 611 Cuts at: 4089 4182 4256 4446 4508 5238 309 Size: 93 74 190 62 730 1535 Fragments arranged by size: 1535 730 611 599 471 335 307 278 243 215 190 120 114 103 93 74 71 62 42 39 35 34 33 28 28 26 15 15 10 8 BspEI T'CCGG_A Cuts at: 923 5684 923 Size: 4761 1703 BspHI T'CATG_A Cuts at: 1550 3326 5640 1550 Size: 1776 2314 2374 Fragments arranged by size: 2374 2314 1776 BspMI ACCTGCnnnn'nnnn_ Cuts at: 590 611 1718 2382 2457 3206 3514 3728 4030 Size: 21 1107 664 75 749 308 214 302 Cuts at: 4030 4411 590 Size: 381 2643 Fragments arranged by size: 2643 1107 749 664 381 308 302 214 75 21 BsrBI CCG'CTC Cuts at: 50 260 915 1548 2412 4756 4810 6294 6459 Size: 210 655 633 864 2344 54 1484 165 Cuts at: 6459 50 Size: 55 Fragments arranged by size: 2344 1484 864 655 633 210 165 55 54 BsrDI GCAATG_nn' Cuts at: 4376 4376 Size: 6464 BsrFI r'CCGG_y Cuts at: 323 733 1994 2589 3410 4462 4643 4852 323 Size: 410 1261 595 821 1052 181 209 1935 Fragments arranged by size: 1935 1261 1052 821 595 410 209 181 BssSI C'ACGA_G Cuts at: 1296 2065 4735 5093 1296 Size: 769 2670 358 2667 Fragments arranged by size: 2670 2667 769 358 BstAPI GCAn_nnn'nTGC Cuts at: 1710 2592 3293 3841 1710 Size: 882 701 548 4333 Fragments arranged by size: 4333 882 701 548 BstBI TT'CG_AA Cuts at: 4825 4825 Size: 6464 BstXI CCAn_nnnn'nTGG Cuts at: 183 697 2172 183 Size: 514 1475 4475 Fragments arranged by size: 4475 1475 514 BstYI r'GATC_y Cuts at: 1 920 1406 1715 1751 1883 2222 3221 3489 Size: 919 486 309 36 132 339 999 268 Cuts at: 3489 3956 3979 4314 4560 5572 5658 5670 6016 Size: 467 23 335 246 1012 86 12 346 Cuts at: 6016 1 Size: 449 Fragments arranged by size: 1012 999 919 486 467 449 346 339 335 309 268 246 132 86 36 23 12 Bsu36I CC'TnA_GG Cuts at: 684 720 1499 1967 684 Size: 36 779 468 5181 Fragments arranged by size: 5181 779 468 36 BtgI C'CryG_G Cuts at: 107 401 611 1385 2534 107 Size: 294 210 774 1149 4037 Fragments arranged by size: 4037 1149 774 294 210 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2496 4414 4595 4758 2496 Size: 1918 181 163 4202 Fragments arranged by size: 4202 1918 181 163 BtsI GCAGTG_nn' Cuts at: 2428 2837 3016 3707 2428 Size: 409 179 691 5185 Fragments arranged by size: 5185 691 409 179 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 1833 1868 1833 Size: 35 6429 DraI TTT'AAA Cuts at: 3463 3463 Size: 6464 DrdI GACnn_nn'nnGTC Cuts at: 987 2047 4170 5028 987 Size: 1060 2123 858 2423 Fragments arranged by size: 2423 2123 1060 858 EaeI y'GGCC_r Cuts at: 803 1283 2174 2381 3392 4049 4223 4614 4641 Size: 480 891 207 1011 657 174 391 27 Cuts at: 4641 5986 803 Size: 1345 1281 Fragments arranged by size: 1345 1281 1011 891 657 480 391 207 174 27 EagI C'GGCC_G Cuts at: 803 4049 803 Size: 3246 3218 EarI CTCTTCn'nnn_ Cuts at: 708 1453 3044 4487 4697 708 Size: 745 1591 1443 210 2475 Fragments arranged by size: 2475 1591 1443 745 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 869 2414 2571 4672 4982 5128 6274 869 Size: 1545 157 2101 310 146 1146 1059 Fragments arranged by size: 2101 1545 1146 1059 310 157 146 EcoICRI GAG'CTC Cuts at: 2730 3476 2730 Size: 746 5718 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 176 246 259 315 840 844 960 1060 1129 Size: 70 13 56 525 4 116 100 69 Cuts at: 1129 1140 1533 1705 1801 2010 2077 2740 2798 Size: 11 393 172 96 209 67 663 58 Cuts at: 2798 3076 3424 4289 4721 5468 5736 176 Size: 278 348 865 432 747 268 904 Fragments arranged by size: 904 865 747 663 525 432 393 348 278 268 209 172 116 100 96 70 69 67 58 56 13 11 4 EcoNI CCTnn'n_nnAGG Cuts at: 1473 1660 1473 Size: 187 6277 EcoO109I rG'GnC_Cy Cuts at: 151 305 407 642 893 894 1424 1495 1669 Size: 154 102 235 251 1 530 71 174 Cuts at: 1669 1932 1963 2169 2229 2646 3317 3603 151 Size: 263 31 206 60 417 671 286 3012 Fragments arranged by size: 3012 671 530 417 286 263 251 235 206 174 154 102 71 60 31 1 EcoRI G'AATT_C Cuts at: 3468 3468 Size: 6464 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1155 1278 2838 4483 1155 Size: 123 1560 1645 3136 Fragments arranged by size: 3136 1645 1560 123 FspI TGC'GCA Cuts at: 1065 2521 2608 4245 1065 Size: 1456 87 1637 3284 Fragments arranged by size: 3284 1637 1456 87 HaeII r_GCGC'y Cuts at: 258 269 330 506 554 666 780 827 1684 Size: 11 61 176 48 112 114 47 857 Cuts at: 1684 1988 2564 3898 4146 5168 6147 258 Size: 304 576 1334 248 1022 979 575 Fragments arranged by size: 1334 1022 979 857 576 575 304 248 176 114 112 61 48 47 11 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1508 1540 1861 1893 2409 2441 4306 4336 4338 Size: 32 321 32 516 32 1865 30 2 Cuts at: 4338 4368 1508 Size: 30 3604 Fragments arranged by size: 3604 1865 516 321 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 437 3503 437 Size: 3066 3398 HindIII A'AGCT_T Cuts at: 2488 2488 Size: 6464 KasI G'GCGC_C Cuts at: 254 550 662 776 1984 3894 4142 254 Size: 296 112 114 1208 1910 248 2576 Fragments arranged by size: 2576 1910 1208 296 248 114 112 KpnI G_GTAC'C Cuts at: 3484 3484 Size: 6464 MluI A'CGCG_T Cuts at: 5897 5897 Size: 6464 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 5135 5319 5135 Size: 184 6280 MscI TGG'CCA Cuts at: 1285 2176 4225 1285 Size: 891 2049 3524 Fragments arranged by size: 3524 2049 891 MslI CAynn'nnrTG Cuts at: 754 2188 2461 2536 2614 2639 2701 4453 4580 Size: 1434 273 75 78 25 62 1752 127 Cuts at: 4580 754 Size: 2638 Fragments arranged by size: 2638 1752 1434 273 127 78 75 62 25 MspA1I CmG'CkG Cuts at: 171 223 252 561 613 675 1107 1127 1292 Size: 52 29 309 52 62 432 20 165 Cuts at: 1292 1511 1920 1928 2331 2691 3271 3363 3435 Size: 219 409 8 403 360 580 92 72 Cuts at: 3435 3575 3889 4249 5262 5507 171 Size: 140 314 360 1013 245 1128 Fragments arranged by size: 1128 1013 580 432 409 403 360 360 314 309 245 219 165 140 92 72 62 52 52 29 20 8 NaeI GCC'GGC Cuts at: 325 735 4645 325 Size: 410 3910 2144 Fragments arranged by size: 3910 2144 410 NarI GG'CG_CC Cuts at: 255 551 663 777 1985 3895 4143 255 Size: 296 112 114 1208 1910 248 2576 Fragments arranged by size: 2576 1910 1208 296 248 114 112 NcoI C'CATG_G Cuts at: 107 2534 107 Size: 2427 4037 NdeI CA'TA_TG Cuts at: 957 957 Size: 6464 NgoMIV G'CCGG_C Cuts at: 323 733 4643 323 Size: 410 3910 2144 Fragments arranged by size: 3910 2144 410 NruI TCG'CGA Cuts at: 835 2429 835 Size: 1594 4870 NspI r_CATG'y Cuts at: 2634 2798 3517 4017 4924 2634 Size: 164 719 500 907 4174 Fragments arranged by size: 4174 907 719 500 164 PasI CC'CwG_GG Cuts at: 309 703 889 1812 2291 2302 3276 309 Size: 394 186 923 479 11 974 3497 Fragments arranged by size: 3497 974 923 479 394 186 11 PciI A'CATG_T Cuts at: 4920 4920 Size: 6464 PflMI CCAn_nnn'nTGG Cuts at: 154 417 867 2291 154 Size: 263 450 1424 4327 Fragments arranged by size: 4327 1424 450 263 PfoI T'CCnGG_A Cuts at: 473 1156 2225 2825 2921 3092 473 Size: 683 1069 600 96 171 3845 Fragments arranged by size: 3845 1069 683 600 171 96 PmeI GTTT'AAAC Cuts at: 3463 3463 Size: 6464 PmlI CAC'GTG Cuts at: 1896 1896 Size: 6464 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5603 5635 5603 Size: 32 6432 PpuMI rG'GwC_Cy Cuts at: 1932 2169 2229 1932 Size: 237 60 6167 Fragments arranged by size: 6167 237 60 PspOMI G'GGCC_C Cuts at: 305 407 893 1669 305 Size: 102 486 776 5100 Fragments arranged by size: 5100 776 486 102 PspXI vC'TCGA_Gb Cuts at: 2350 2350 Size: 6464 PstI C_TGCA'G Cuts at: 1346 2393 3511 4196 1346 Size: 1047 1118 685 3614 Fragments arranged by size: 3614 1118 1047 685 PvuI CG_AT'CG Cuts at: 104 104 Size: 6464 PvuII CAG'CTG Cuts at: 1107 1292 1920 3889 4249 1107 Size: 185 628 1969 360 3322 Fragments arranged by size: 3322 1969 628 360 185 RsrII CG'GwC_CG Cuts at: 4659 4659 Size: 6464 SacI G_AGCT'C Cuts at: 2732 3478 2732 Size: 746 5718 SacII CC_GC'GG Cuts at: 614 614 Size: 6464 SalI G'TCGA_C Cuts at: 3501 3501 Size: 6464 SapI GCTCTTCn'nnn_ Cuts at: 708 3044 4487 4697 708 Size: 2336 1443 210 2475 Fragments arranged by size: 2475 2336 1443 210 SbfI CC_TGCA'GG Cuts at: 3511 3511 Size: 6464 ScaI AGT'ACT Cuts at: 1021 5981 1021 Size: 4960 1504 SfcI C'TryA_G Cuts at: 33 1342 2389 2853 3507 4192 5185 5376 33 Size: 1309 1047 464 654 685 993 191 1121 Fragments arranged by size: 1309 1121 1047 993 685 654 464 191 SfiI GGCCn_nnn'nGGCC Cuts at: 707 707 Size: 6464 SfoI GGC'GCC Cuts at: 256 552 664 778 1986 3896 4144 256 Size: 296 112 114 1208 1910 248 2576 Fragments arranged by size: 2576 1910 1208 296 248 114 112 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 6464 SgrAI Cr'CCGG_yG Cuts at: 323 323 Size: 6464 SmaI CCC'GGG Cuts at: 3486 3486 Size: 6464 SmlI C'TyrA_G Cuts at: 2072 2350 2671 3041 3619 4534 5026 5288 2072 Size: 278 321 370 578 915 492 262 3248 Fragments arranged by size: 3248 915 578 492 370 321 278 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 6464 SphI G_CATG'C Cuts at: 2634 3517 4017 2634 Size: 883 500 5081 Fragments arranged by size: 5081 883 500 StuI AGG'CCT Cuts at: 232 817 2680 232 Size: 585 1863 4016 Fragments arranged by size: 4016 1863 585 StyI C'CwwG_G Cuts at: 107 1673 2165 2450 2534 3598 3952 107 Size: 1566 492 285 84 1064 354 2619 Fragments arranged by size: 2619 1566 1064 492 354 285 84 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 409 1988 2879 3018 4047 4180 4639 4848 409 Size: 1579 891 139 1029 133 459 209 2025 Fragments arranged by size: 2025 1579 1029 891 459 209 139 133 TatI w'GTAC_w Cuts at: 928 1019 5979 928 Size: 91 4960 1413 Fragments arranged by size: 4960 1413 91 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 1207 1239 5981 6013 1207 Size: 32 4742 32 1658 Fragments arranged by size: 4742 1658 32 32 Tth111I GACn'n_nGTC Cuts at: 4261 4261 Size: 6464 XbaI T'CTAG_A Cuts at: 3495 3495 Size: 6464 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1680 1809 2376 3959 1680 Size: 129 567 1583 4185 Fragments arranged by size: 4185 1583 567 129 XhoI C'TCGA_G Cuts at: 2072 2350 2072 Size: 278 6186 XmaI C'CCGG_G Cuts at: 3484 3484 Size: 6464 XmnI GAAnn'nnTTC Cuts at: 1243 3079 1243 Size: 1836 4628 ZraI GAC'GTC Cuts at: 2375 2375 Size: 6464 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NruI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PspOMI PspXI PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AflII AloI AscI AvrII BaeI BfrBI BmtI BsaXI BsiWI BsmI BsrGI BssHII BstEII BstZ17I ClaI DraIII EcoRV FseI FspAI HpaI MfeI NheI NotI NsiI PacI PshAI PsiI PsrI SanDI SexAI SnaBI SrfI SspI SwaI