(Linear) (Six-base) MAPSORT of: KIEE2742.seq Check: 1476 from: 1 to: 4083 KIEE2742.orf With 182 enzymes: SgfI * June 19, 2019 14:13 .. AatII G_ACGT'C Cuts at: 0 2882 4083 Size: 2882 1201 AccI GT'mk_AC Cuts at: 0 1127 1694 2882 3572 4083 Size: 1127 567 1188 690 511 Fragments arranged by size: 1188 1127 690 567 511 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 439 824 1651 2338 3322 3439 3774 4083 Size: 439 385 827 687 984 117 335 309 Fragments arranged by size: 984 827 687 439 385 335 309 117 AgeI A'CCGG_T Cuts at: 0 614 3132 4083 Size: 614 2518 951 Fragments arranged by size: 2518 951 614 AleI CACnn'nnGTG Cuts at: 0 509 1252 4083 Size: 509 743 2831 Fragments arranged by size: 2831 743 509 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 205 4083 Size: 205 3878 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 554 586 641 673 4083 Size: 554 32 55 32 3410 Fragments arranged by size: 3410 554 55 32 32 AlwNI CAG_nnn'CTG Cuts at: 0 97 1178 1382 1650 2068 3527 4083 Size: 97 1081 204 268 418 1459 556 Fragments arranged by size: 1459 1081 556 418 268 204 97 ApaI G_GGCC'C Cuts at: 0 2116 2569 2828 4083 Size: 2116 453 259 1255 Fragments arranged by size: 2116 1255 453 259 ApoI r'AATT_y Cuts at: 0 1025 2167 2613 2630 4083 Size: 1025 1142 446 17 1453 Fragments arranged by size: 1453 1142 1025 446 17 AvaI C'yCGr_G Cuts at: 0 890 2324 4083 Size: 890 1434 1759 Fragments arranged by size: 1759 1434 890 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 1301 1334 1405 1438 4083 Size: 1301 33 71 33 2645 Fragments arranged by size: 2645 1301 71 33 33 BamHI G'GATC_C Cuts at: 0 402 689 3230 4083 Size: 402 287 2541 853 Fragments arranged by size: 2541 853 402 287 BanI G'GyrC_C Cuts at: 0 104 675 2945 3259 4083 Size: 104 571 2270 314 824 Fragments arranged by size: 2270 824 571 314 104 BanII G_rGCy'C Cuts at: 0 121 2116 2569 2828 3245 4083 Size: 121 1995 453 259 417 838 Fragments arranged by size: 1995 838 453 417 259 121 BbsI GAAGACnn'nnnn_ Cuts at: 0 854 3318 4083 Size: 854 2464 765 Fragments arranged by size: 2464 854 765 BbvCI CC'TCA_GC Cuts at: 0 1111 4083 Size: 1111 2972 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 261 295 2227 2261 4083 Size: 261 34 1932 34 1822 Fragments arranged by size: 1932 1822 261 34 34 BclI T'GATC_A Cuts at: 0 1796 1949 2210 4083 Size: 1796 153 261 1873 Fragments arranged by size: 1873 1796 261 153 BglI GCCn_nnn'nGGC Cuts at: 0 748 1221 2573 4083 Size: 748 473 1352 1510 Fragments arranged by size: 1510 1352 748 473 Bme1580I G_kGCm'C Cuts at: 0 109 336 680 1624 2116 2569 2828 4055 Size: 109 227 344 944 492 453 259 1227 Cuts at: 4055 4083 Size: 28 Fragments arranged by size: 1227 944 492 453 344 259 227 109 28 BmgBI CAC'GTC Cuts at: 0 966 3576 4083 Size: 966 2610 507 Fragments arranged by size: 2610 966 507 BmrI ACTGGGnnnn_n' Cuts at: 0 2170 3177 3399 3713 4083 Size: 2170 1007 222 314 370 Fragments arranged by size: 2170 1007 370 314 222 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1126 3259 4083 Size: 1126 2133 824 Fragments arranged by size: 2133 1126 824 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1325 2713 2994 3109 3879 4083 Size: 1325 1388 281 115 770 204 Fragments arranged by size: 1388 1325 770 281 204 115 Bpu10I CC'TnA_GC Cuts at: 0 1111 4083 Size: 1111 2972 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 472 3931 4083 Size: 472 3459 152 Fragments arranged by size: 3459 472 152 BsaI GGTCTCn'nnnn_ Cuts at: 0 3949 4083 Size: 3949 134 BsaAI yAC'GTr Cuts at: 0 2103 4083 Size: 2103 1980 BsaHI Gr'CG_yC Cuts at: 0 2306 2879 3323 3518 4083 Size: 2306 573 444 195 565 Fragments arranged by size: 2306 573 565 444 195 BsaWI w'CCGG_w Cuts at: 0 614 3132 4083 Size: 614 2518 951 Fragments arranged by size: 2518 951 614 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 554 584 1176 1206 2467 2497 2893 2923 Size: 554 30 592 30 1261 30 396 30 Cuts at: 2923 4083 Size: 1160 Fragments arranged by size: 1261 1160 592 554 396 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 326 1453 1903 4083 Size: 326 1127 450 2180 Fragments arranged by size: 2180 1127 450 326 BseYI C'CCAG_C Cuts at: 0 10 100 325 548 3070 4083 Size: 10 90 225 223 2522 1013 Fragments arranged by size: 2522 1013 225 223 90 10 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 766 1411 2652 3513 4083 Size: 766 645 1241 861 570 Fragments arranged by size: 1241 861 766 645 570 BsiEI CG_ry'CG Cuts at: 0 1167 2263 4083 Size: 1167 1096 1820 Fragments arranged by size: 1820 1167 1096 BsiHKAI G_wGCw'C Cuts at: 0 2153 2187 3112 4083 Size: 2153 34 925 971 Fragments arranged by size: 2153 971 925 34 BsmI GAATG_Cn' Cuts at: 0 3967 4083 Size: 3967 116 BsmBI CGTCTCn'nnnn_ Cuts at: 0 3897 4083 Size: 3897 186 Bsp1286I G_dGCh'C Cuts at: 0 109 121 336 680 1624 2116 2153 2187 Size: 109 12 215 344 944 492 37 34 Cuts at: 2187 2569 2828 3112 3245 4055 4083 Size: 382 259 284 133 810 28 Fragments arranged by size: 944 810 492 382 344 284 259 215 133 109 37 34 28 12 BspHI T'CATG_A Cuts at: 0 990 4083 Size: 990 3093 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 76 1433 3047 4083 Size: 76 1357 1614 1036 Fragments arranged by size: 1614 1357 1036 76 BsrDI GCAATG_nn' Cuts at: 0 1319 2007 2351 2771 4083 Size: 1319 688 344 420 1312 Fragments arranged by size: 1319 1312 688 420 344 BsrFI r'CCGG_y Cuts at: 0 614 1684 2331 2417 2699 2767 3132 4083 Size: 614 1070 647 86 282 68 365 951 Fragments arranged by size: 1070 951 647 614 365 282 86 68 BsrGI T'GTAC_A Cuts at: 0 1287 3011 4083 Size: 1287 1724 1072 Fragments arranged by size: 1724 1287 1072 BssSI C'ACGA_G Cuts at: 0 3783 4083 Size: 3783 300 BstAPI GCAn_nnn'nTGC Cuts at: 0 1178 1382 1940 2996 4083 Size: 1178 204 558 1056 1087 Fragments arranged by size: 1178 1087 1056 558 204 BstXI CCAn_nnnn'nTGG Cuts at: 0 1095 1885 2715 3918 4062 4083 Size: 1095 790 830 1203 144 21 Fragments arranged by size: 1203 1095 830 790 144 21 BstYI r'GATC_y Cuts at: 0 402 631 689 1378 2065 2558 3230 4083 Size: 402 229 58 689 687 493 672 853 Fragments arranged by size: 853 689 687 672 493 402 229 58 BtgI C'CryG_G Cuts at: 0 209 558 1776 2120 2373 3817 3904 4083 Size: 209 349 1218 344 253 1444 87 179 Fragments arranged by size: 1444 1218 349 344 253 209 179 87 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 766 4083 Size: 766 3317 BtsI GCAGTG_nn' Cuts at: 0 187 710 1830 2997 4083 Size: 187 523 1120 1167 1086 Fragments arranged by size: 1167 1120 1086 523 187 DraIII CAC_nnn'GTG Cuts at: 0 165 4083 Size: 165 3918 DrdI GACnn_nn'nnGTC Cuts at: 0 2224 4083 Size: 2224 1859 EaeI y'GGCC_r Cuts at: 0 144 174 740 1682 2024 2260 2333 2450 Size: 144 30 566 942 342 236 73 117 Cuts at: 2450 4083 Size: 1633 Fragments arranged by size: 1633 942 566 342 236 144 117 73 30 EagI C'GGCC_G Cuts at: 0 2260 4083 Size: 2260 1823 EarI CTCTTCn'nnn_ Cuts at: 0 34 207 1349 4083 Size: 34 173 1142 2734 Fragments arranged by size: 2734 1142 173 34 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1480 2713 4083 Size: 1480 1233 1370 Fragments arranged by size: 1480 1370 1233 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 439 824 1325 1651 2338 2713 2994 3109 Size: 439 385 501 326 687 375 281 115 Cuts at: 3109 3322 3439 3774 3879 4083 Size: 213 117 335 105 204 Fragments arranged by size: 687 501 439 385 375 335 326 281 213 204 117 115 105 EcoNI CCTnn'n_nnAGG Cuts at: 0 31 3148 4083 Size: 31 3117 935 Fragments arranged by size: 3117 935 31 EcoO109I rG'GnC_Cy Cuts at: 0 1734 2113 2378 2565 2566 2594 2825 3335 Size: 1734 379 265 187 1 28 231 510 Cuts at: 3335 4083 Size: 748 Fragments arranged by size: 1734 748 510 379 265 231 187 28 1 EcoRI G'AATT_C Cuts at: 0 2630 4083 Size: 2630 1453 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 2523 3081 3612 3678 4083 Size: 2523 558 531 66 405 Fragments arranged by size: 2523 558 531 405 66 HaeII r_GCGC'y Cuts at: 0 449 2714 3100 4083 Size: 449 2265 386 983 Fragments arranged by size: 2265 983 449 386 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 574 606 636 668 985 1017 2013 2045 Size: 574 32 30 32 317 32 996 32 Cuts at: 2045 2467 2499 2848 2880 3385 3417 3480 3512 Size: 422 32 349 32 505 32 63 32 Cuts at: 3512 4083 Size: 571 Fragments arranged by size: 996 574 571 505 422 349 317 63 32 32 32 32 32 32 32 32 30 HincII GTy'rAC Cuts at: 0 1107 2883 4083 Size: 1107 1776 1200 Fragments arranged by size: 1776 1200 1107 HindIII A'AGCT_T Cuts at: 0 3925 4083 Size: 3925 158 HpaI GTT'AAC Cuts at: 0 1107 4083 Size: 1107 2976 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 2216 4083 Size: 2216 1867 MscI TGG'CCA Cuts at: 0 146 742 2026 2452 4083 Size: 146 596 1284 426 1631 Fragments arranged by size: 1631 1284 596 426 146 MslI CAynn'nnrTG Cuts at: 0 509 1093 1252 1495 1498 3697 3739 4003 Size: 509 584 159 243 3 2199 42 264 Cuts at: 4003 4060 4083 Size: 57 23 Fragments arranged by size: 2199 584 509 264 243 159 57 42 23 3 MspA1I CmG'CkG Cuts at: 0 53 1650 2059 2316 2414 2841 3609 4083 Size: 53 1597 409 257 98 427 768 474 Fragments arranged by size: 1597 768 474 427 409 257 98 53 NaeI GCC'GGC Cuts at: 0 2333 2701 4083 Size: 2333 368 1382 Fragments arranged by size: 2333 1382 368 NcoI C'CATG_G Cuts at: 0 1776 3817 3904 4083 Size: 1776 2041 87 179 Fragments arranged by size: 2041 1776 179 87 NdeI CA'TA_TG Cuts at: 0 1637 4083 Size: 1637 2446 NgoMIV G'CCGG_C Cuts at: 0 2331 2699 4083 Size: 2331 368 1384 Fragments arranged by size: 2331 1384 368 NspI r_CATG'y Cuts at: 0 325 1461 4083 Size: 325 1136 2622 Fragments arranged by size: 2622 1136 325 PasI CC'CwG_GG Cuts at: 0 76 1969 4083 Size: 76 1893 2114 Fragments arranged by size: 2114 1893 76 PflMI CCAn_nnn'nTGG Cuts at: 0 53 953 2018 3167 3630 3744 4083 Size: 53 900 1065 1149 463 114 339 Fragments arranged by size: 1149 1065 900 463 339 114 53 PfoI T'CCnGG_A Cuts at: 0 478 4083 Size: 478 3605 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 554 586 4083 Size: 554 32 3497 Fragments arranged by size: 3497 554 32 PpuMI rG'GwC_Cy Cuts at: 0 1734 2594 3335 4083 Size: 1734 860 741 748 Fragments arranged by size: 1734 860 748 741 PshAI GACnn'nnGTC Cuts at: 0 1880 3322 3941 4083 Size: 1880 1442 619 142 Fragments arranged by size: 1880 1442 619 142 PspOMI G'GGCC_C Cuts at: 0 2112 2565 2824 4083 Size: 2112 453 259 1259 Fragments arranged by size: 2112 1259 453 259 PstI C_TGCA'G Cuts at: 0 1378 2064 3149 4083 Size: 1378 686 1085 934 Fragments arranged by size: 1378 1085 934 686 PvuII CAG'CTG Cuts at: 0 53 1650 2414 2841 3609 4083 Size: 53 1597 764 427 768 474 Fragments arranged by size: 1597 768 764 474 427 53 RsrII CG'GwC_CG Cuts at: 0 1923 4083 Size: 1923 2160 SalI G'TCGA_C Cuts at: 0 2881 4083 Size: 2881 1202 ScaI AGT'ACT Cuts at: 0 1329 4083 Size: 1329 2754 SexAI A'CCwGG_T Cuts at: 0 1394 4083 Size: 1394 2689 SfcI C'TryA_G Cuts at: 0 627 1374 1530 2060 2603 3145 4083 Size: 627 747 156 530 543 542 938 Fragments arranged by size: 938 747 627 543 542 530 156 SmaI CCC'GGG Cuts at: 0 892 2326 4083 Size: 892 1434 1757 Fragments arranged by size: 1757 1434 892 SmlI C'TyrA_G Cuts at: 0 451 3946 4083 Size: 451 3495 137 Fragments arranged by size: 3495 451 137 SphI G_CATG'C Cuts at: 0 325 4083 Size: 325 3758 SspI AAT'ATT Cuts at: 0 928 4083 Size: 928 3155 StuI AGG'CCT Cuts at: 0 2242 4083 Size: 2242 1841 StyI C'CwwG_G Cuts at: 0 786 802 1776 2249 3762 3817 3904 4083 Size: 786 16 974 473 1513 55 87 179 Fragments arranged by size: 1513 974 786 473 179 87 55 16 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 92 521 1064 1734 2963 2984 3127 3993 Size: 92 429 543 670 1229 21 143 866 Cuts at: 3993 4083 Size: 90 Fragments arranged by size: 1229 866 670 543 429 143 92 90 21 TatI w'GTAC_w Cuts at: 0 422 1287 1327 2129 3011 4083 Size: 422 865 40 802 882 1072 Fragments arranged by size: 1072 882 865 802 422 40 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1176 1208 4083 Size: 1176 32 2875 Fragments arranged by size: 2875 1176 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 90 916 1272 1783 2019 3867 4083 Size: 90 826 356 511 236 1848 216 Fragments arranged by size: 1848 826 511 356 236 216 90 XmaI C'CCGG_G Cuts at: 0 890 2324 4083 Size: 890 1434 1759 Fragments arranged by size: 1759 1434 890 XmnI GAAnn'nnTTC Cuts at: 0 392 3064 3682 4083 Size: 392 2672 618 401 Fragments arranged by size: 2672 618 401 392 ZraI GAC'GTC Cuts at: 0 2880 4083 Size: 2880 1203 Enzymes that do cut: AatII AccI AcuI AgeI AleI AlfI AloI AlwNI ApaI ApoI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BclI BglI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrDI BsrFI BsrGI BssSI BstAPI BstXI BstYI BtgI BtgZI BtsI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII HindIII HpaI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NspI PasI PflMI PfoI PpiI PpuMI PshAI PspOMI PstI PvuII RsrII SalI ScaI SexAI SfcI SmaI SmlI SphI SspI StuI StyI TaqII TatI TstI XcmI XmaI XmnI ZraI Enzymes that do not cut: AarI Acc65I AclI AfeI AflII AflIII AhdI ApaLI AscI AseI AsiSI AvrII BbeI BciVI BfrBI BglII BlpI BmtI BsaBI BsiWI BspEI BsrBI BssHII BstBI BstEII BstZ17I Bsu36I ClaI CspCI DraI EcoICRI EcoRV FseI FspI FspAI KasI KpnI MfeI MluI NarI NheI NotI NruI NsiI PacI PciI PmeI PmlI PsiI PspXI PsrI PvuI SacI SacII SanDI SapI SbfI SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI SwaI Tth111I XbaI XhoI