################################## # NO CUT ENZYMES ( 92 enzymes) # ################################## AatII AccI AccIII AclI AcyI AflII AflIII AgeI Alw44I ApaLI AscI Asp700I AspI AvrII BamHI BbeI BcgI BfrI BglI BglII BlnI BsaHI BsaWI BseAI BsgI BsiEI BsiWI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrGI BssHII BssSI Bst1107I BstAPI BstZ17I ClaI CpoI DrdI EagI EclXI Eco52I EcoNI EcoRI EcoT22I FseI Hin1I HincII HindII HpaI KasI KspI MfeI MluI MroI MunI NarI NcoI NdeI NheI NotI NruI NsiI PacI PflFI PinAI PmeI PpuMI Psp1406I PvuI RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SpeI SphI Sse8387I SspBI SspI SwaI Tth111I XbaI XmnI ################################### # ONE CUT ENZYMES ( 82 enzymes) # ################################### Acc65I 666 GGTACC G^GTACC AhdI 680 GACCAGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 236 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 1036 CCTAAGG CC^T[ACGT]AGG Aor51HI 741 AGCGCT AGC^GCT ApaI 696 GGGCCC GGGCC^C AseI 967 ATTAAT AT^TAAT AsnI 967 ATTAAT AT^TAAT Asp718I 666 GGTACC G^GTACC AspEI 680 GACCAGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 119 TGCGCA TGC^GCA BalI 248 TGGCCA TGG^CCA BanII 696 GGGCCC G[GA]GC[CT]^C BbrPI 1567 CACGTG CAC^GTG BbsI 636 GAAGAC GAAGAC(2/6) BbvCI 1436 CCTCAGC CCTCAGC(-5/-2) BciVI 960 GGATAC GTATCC(6/5) BclI 1335 TGATCA T^GATCA BlpI 884 GCTTAGC GC^T[ACGT]AGC Bpu1102I 884 GCTTAGC GC^T[ACGT]AGC BpuAI 636 GAAGAC GAAGAC(2/6) BsaBI 780 GATATCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 1612 GAGACC GGTCTC(1/5) BsmBI 1607 GAGACG CGTCTC(1/5) BsmI 620 GAATGC GAATGC(1/-1) BsrDI 935 CATTGC GCAATG(2/0) BsrFI 26 GCCGGC [GA]^CCGG[CT] BstBI 259 TTCGAA TT^CGAA BstEII 795 GGTCACC G^GT[ACGT]ACC BstPI 795 GGTCACC G^GT[ACGT]ACC BstXI 1455 CCATCGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 865 AGATCC [GA]^GATC[CT] Bsu36I 1036 CCTAAGG CC^T[ACGT]AGG CelII 884 GCTTAGC GC^T[ACGT]AGC Cfr10I 26 GCCGGC [GA]^CCGG[CT] DraI 756 TTTAAA TTT^AAA DraII 696 GGGCCCT [GA]G^G[ACGT]CC[CT] DraIII 1586 CACACCGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 1617 CCACGG C^C[GA][CT]GG EaeI 248 TGGCCA [CT]^GGCC[GA] Eam1105I 680 GACCAGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 1428 CTCTTC CTCTTC(1/4) Eco47III 741 AGCGCT AGC^GCT Eco81I 1036 CCTAAGG CC^T[ACGT]AGG EcoO109I 696 GGGCCCT [GA]G^G[ACGT]CC[CT] EcoO65I 795 GGTCACC G^GT[ACGT]ACC FauI 11 GCGGG CCCGC(4/6) FbaI 1335 TGATCA T^GATCA FspI 119 TGCGCA TGC^GCA HaeII 741 AGCGCT [GA]GCGC^[CT] HgaI 955 GACGC GACGC(5/10) HindIII 882 AAGCTT A^AGCTT KpnI 666 GGTACC GGTAC^C Ksp632I 1428 CTCTTC CTCTTC(1/4) MamI 780 GATATCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 865 AGATCC [GA]^GATC[CT] MluNI 248 TGGCCA TGG^CCA MscI 248 TGGCCA TGG^CCA NaeI 26 GCCGGC GCC^GGC NgoMIV 26 GCCGGC G^CCGGC NspI 279 GCATGT [GA]CATG^[CT] NspV 259 TTCGAA TT^CGAA PaeR7I 1064 CTCGAG C^TCGAG PflMI 235 CCAGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 1567 CACGTG CAC^GTG PmlI 1567 CACGTG CAC^GTG PshAI 195 GACAAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 967 ATTAAT AT^TAAT PstI 385 CTGCAG CTGCA^G PvuII 1109 CAGCTG CAG^CTG SapI 1427 GCTCTTC GCTCTTC(1/4) SfuI 259 TTCGAA TT^CGAA SgrAI 25 CGCCGGCG C[GA]^CCGG[CT]G SmaI 1661 CCCGGG CCC^GGG SnaBI 1534 TACGTA TAC^GTA StuI 1208 AGGCCT AGG^CCT Van91I 235 CCAGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 674 CCATGCGACCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 1064 CTCGAG C^TCGAG XhoII 865 AGATCC [GA]^GATC[CT] XmaCI 1661 CCCGGG C^CCGGG XmaI 1661 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AccII 957 CGCG CG^CG 1474 CGCG AspHI 643 GAGCAC G[AT]GC[AT]^C 1186 GAGCAC AvaI 1064 CTCGAG C^[CT]CG[GA]G 1661 CCCGGG BanI 666 GGTACC G^G[CT][GA]CC 1652 GGCACC BsaAI 1534 TACGTA [CT]AC^GT[GA] 1567 CACGTG BsiHKAI 643 GAGCAC G[AT]GC[AT]^C 1186 GAGCAC BsoBI 1064 CTCGAG C^[CT]CG[GA]G 1661 CCCGGG BstUI 957 CGCG CG^CG 1474 CGCG EcoRV 780 GATATC GAT^ATC 1758 GATATC EcoT14I 998 CCTTGG C^C[AT][AT]GG 1724 CCAAGG MspA1I 800 CCGCTG C[AC]G^C[GT]G 1109 CAGCTG MvnI 957 CGCG CG^CG 1474 CGCG StyI 998 CCTTGG C^C[AT][AT]GG 1724 CCAAGG TfiI 82 GATTC G^A[AT]TC 347 GATTC ######################################### # 3 OR MORE CUT ENZYMES ( 70 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI AvaII BbvI BcnI BfaI BmyI BpmI BsaJI BseRI BsiYI BslI BsmAI BsmFI Bsp1286I BsrBI BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII EcoRII Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I