Restriction map for
pF1KE3356
(Circular) (Six-base) MAPSORT of: pF1KE3356.seq Check: 1187 from: 1 to: 6257
pF1KE3356 6257 bp
With 182 enzymes: SgfI *
October 24, 2019 20:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1830 1830
Size: 6257
AccI GT'mk_AC
Cuts at: 2361 3295 2361
Size: 934 5323
Acc65I G'GTAC_C
Cuts at: 841 3273 841
Size: 2432 3825
AclI AA'CG_TT
Cuts at: 6097 6097
Size: 6257
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1451 1721 1754 2507 2581 2887 4082 4514 5261
Size: 270 33 753 74 306 1195 432 747
Cuts at: 5261 5529 1451
Size: 268 2179
Fragments arranged by size:
2179 1195 753 747 432 306 270 268
74 33
AfeI AGC'GCT
Cuts at: 1875 1875
Size: 6257
AflII C'TTAA_G
Cuts at: 1834 1834
Size: 6257
AflIII A'CryG_T
Cuts at: 354 584 1363 3609 4713 5690 354
Size: 230 779 2246 1104 977 921
Fragments arranged by size:
2246 1104 977 921 779 230
AgeI A'CCGG_T
Cuts at: 4645 4645
Size: 6257
AleI CACnn'nnGTG
Cuts at: 3103 3103
Size: 6257
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2142 2142
Size: 6257
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 295 327 441 473 295
Size: 32 114 32 6079
Fragments arranged by size:
6079 114 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2298 3003 3562 5129 2298
Size: 705 559 1567 3426
Fragments arranged by size:
3426 1567 705 559
ApaLI G'TGCA_C
Cuts at: 721 817 5027 721
Size: 96 4210 1951
Fragments arranged by size:
4210 1951 96
ApoI r'AATT_y
Cuts at: 487 1612 1773 2169 2839 3207 3261 5610 5866
Size: 1125 161 396 670 368 54 2349 256
Cuts at: 5866 487
Size: 878
Fragments arranged by size:
2349 1125 878 670 396 368 256 161
54
AseI AT'TA_AT
Cuts at: 20 20
Size: 6257
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6257
AvaI C'yCGr_G
Cuts at: 888 2677 2902 3277 888
Size: 1789 225 375 3868
Fragments arranged by size:
3868 1789 375 225
BamHI G'GATC_C
Cuts at: 1197 3282 1197
Size: 2085 4172
BanI G'GyrC_C
Cuts at: 841 1795 2784 3273 3687 3935 3970 841
Size: 954 989 489 414 248 35 3128
Fragments arranged by size:
3128 989 954 489 414 248 35
BanII G_rGCy'C
Cuts at: 2187 2494 2852 3031 3271 4301 2187
Size: 307 358 179 240 1030 4143
Fragments arranged by size:
4143 1030 358 307 240 179
BbeI G_GCGC'C
Cuts at: 3691 3939 3691
Size: 248 6009
BbsI GAAGACnn'nnnn_
Cuts at: 347 1439 1645 347
Size: 1092 206 4959
Fragments arranged by size:
4959 1092 206
BbvCI CC'TCA_GC
Cuts at: 971 971
Size: 6257
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 1121 1155 1678 1712 3113 3147 90
Size: 34 997 34 523 34 1401 34 3200
Fragments arranged by size:
3200 1401 997 523 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2999 4155 4916 6257 2999
Size: 1156 761 1341 2999
Fragments arranged by size:
2999 1341 1156 761
BclI T'GATC_A
Cuts at: 3777 3777
Size: 6257
BfrBI ATG'CAT
Cuts at: 965 965
Size: 6257
BglII A'GATC_T
Cuts at: 3772 5809 3772
Size: 2037 4220
BlpI GC'TnA_GC
Cuts at: 3369 3369
Size: 6257
Bme1580I G_kGCm'C
Cuts at: 725 821 2787 3882 3975 5031 725
Size: 96 1966 1095 93 1056 1951
Fragments arranged by size:
1966 1951 1095 1056 96 93
BmgBI CAC'GTC
Cuts at: 1364 1364
Size: 6257
BmrI ACTGGGnnnn_n'
Cuts at: 1205 3469 3550 3885 6029 1205
Size: 2264 81 335 2144 1433
Fragments arranged by size:
2264 2144 1433 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 756 4624 756
Size: 3868 2389
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1272 2320 3015 1272
Size: 1048 695 4514
Fragments arranged by size:
4514 1048 695
Bpu10I CC'TnA_GC
Cuts at: 971 3025 971
Size: 2054 4203
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 939 3433 4312 4804 5102 939
Size: 2494 879 492 298 2094
Fragments arranged by size:
2494 2094 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 915 2107 5961 915
Size: 1192 3854 1211
Fragments arranged by size:
3854 1211 1192
BsaAI yAC'GTr
Cuts at: 3610 3610
Size: 6257
BsaBI GATnn'nnATC
Cuts at: 6 3771 5808 6
Size: 3765 2037 455
Fragments arranged by size:
3765 2037 455
BsaHI Gr'CG_yC
Cuts at: 3688 3936 4638 6134 3688
Size: 248 702 1496 3811
Fragments arranged by size:
3811 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1307 2058 2368 2658 3446 3967 4645 4919 5066
Size: 751 310 290 788 521 678 274 147
Cuts at: 5066 5477 1307
Size: 411 2087
Fragments arranged by size:
2087 788 751 678 521 411 310 290
274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 441 471 598 628 849 879 1353 1383 2040
Size: 30 127 30 221 30 474 30 657
Cuts at: 2040 2070 441
Size: 30 4628
Fragments arranged by size:
4628 657 474 221 127 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 732 2042 2045 732
Size: 1310 3 4944
Fragments arranged by size:
4944 1310 3
BseYI C'CCAG_C
Cuts at: 2388 3682 4440 5017 5513 2388
Size: 1294 758 577 496 3132
Fragments arranged by size:
3132 1294 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1151 2639 1151
Size: 1488 4769
BsiEI CG_ry'CG
Cuts at: 104 731 3799 3845 5053 104
Size: 627 3068 46 1208 1308
Fragments arranged by size:
3068 1308 1208 627 46
BsiHKAI G_wGCw'C
Cuts at: 725 809 821 1072 3031 3271 4049 4239 5031
Size: 84 12 251 1959 240 778 190 792
Cuts at: 5031 725
Size: 1951
Fragments arranged by size:
1959 1951 792 778 251 240 190 84
12
BsmI GAATG_Cn'
Cuts at: 884 3225 884
Size: 2341 3916
Bsp1286I G_dGCh'C
Cuts at: 725 809 821 1072 2187 2494 2787 2852 3031
Size: 84 12 251 1115 307 293 65 179
Cuts at: 3031 3271 3882 3975 4049 4239 4301 5031 725
Size: 240 611 93 74 190 62 730 1951
Fragments arranged by size:
1951 1115 730 611 307 293 251 240
190 179 93 84 74 65 62 12
BspEI T'CCGG_A
Cuts at: 2058 5477 2058
Size: 3419 2838
BspHI T'CATG_A
Cuts at: 5433 5433
Size: 6257
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1830 3307 3521 3823 4204 1830
Size: 1477 214 302 381 3883
Fragments arranged by size:
3883 1477 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4549 4603 6087 6252 50
Size: 4499 54 1484 165 55
Fragments arranged by size:
4499 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 4169 4169
Size: 6257
BsrFI r'CCGG_y
Cuts at: 371 1315 4255 4436 4645 371
Size: 944 2940 181 209 1983
Fragments arranged by size:
2940 1983 944 209 181
BsrGI T'GTAC_A
Cuts at: 155 155
Size: 6257
BssHII G'CGCG_C
Cuts at: 851 851
Size: 6257
BssSI C'ACGA_G
Cuts at: 4528 4886 4528
Size: 358 5899
BstAPI GCAn_nnn'nTGC
Cuts at: 314 3003 3634 314
Size: 2689 631 2937
Fragments arranged by size:
2937 2689 631
BstBI TT'CG_AA
Cuts at: 4618 4618
Size: 6257
BstXI CCAn_nnnn'nTGG
Cuts at: 1341 1341
Size: 6257
BstYI r'GATC_y
Cuts at: 1 539 1197 1438 3282 3749 3772 4107 4353
Size: 538 658 241 1844 467 23 335 246
Cuts at: 4353 5365 5451 5463 5809 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1844 1012 658 538 467 449 346 335
246 241 86 23 12
BtgI C'CryG_G
Cuts at: 1170 1187 1406 2177 2779 1170
Size: 17 219 771 602 4648
Fragments arranged by size:
4648 771 602 219 17
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4207 4388 4551 4207
Size: 181 163 5913
Fragments arranged by size:
5913 181 163
BtsI GCAGTG_nn'
Cuts at: 1233 3500 1233
Size: 2267 3990
DraI TTT'AAA
Cuts at: 2168 3256 2168
Size: 1088 5169
DrdI GACnn_nn'nnGTC
Cuts at: 3963 4821 3963
Size: 858 5399
EaeI y'GGCC_r
Cuts at: 728 3842 4016 4407 4434 5779 728
Size: 3114 174 391 27 1345 1206
Fragments arranged by size:
3114 1345 1206 391 174 27
EagI C'GGCC_G
Cuts at: 728 3842 728
Size: 3114 3143
EarI CTCTTCn'nnn_
Cuts at: 2192 2702 2790 2913 4280 4490 2192
Size: 510 88 123 1367 210 3959
Fragments arranged by size:
3959 1367 510 210 123 88
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2056 4465 4775 4921 6067 2056
Size: 2409 310 146 1146 2246
Fragments arranged by size:
2409 2246 1146 310 146
EcoICRI GAG'CTC
Cuts at: 3029 3269 3029
Size: 240 6017
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1272 1451 1721 1754 2320 2507 2581 2887 3015
Size: 179 270 33 566 187 74 306 128
Cuts at: 3015 4082 4514 5261 5529 1272
Size: 1067 432 747 268 2000
Fragments arranged by size:
2000 1067 747 566 432 306 270 268
187 179 128 74 33
EcoNI CCTnn'n_nnAGG
Cuts at: 2501 2501
Size: 6257
EcoO109I rG'GnC_Cy
Cuts at: 2511 3396 2511
Size: 885 5372
EcoRI G'AATT_C
Cuts at: 3261 3261
Size: 6257
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4276 4276
Size: 6257
FspI TGC'GCA
Cuts at: 1763 4038 1763
Size: 2275 3982
HaeII r_GCGC'y
Cuts at: 1183 1877 3004 3691 3939 4961 5940 1183
Size: 694 1127 687 248 1022 979 1500
Fragments arranged by size:
1500 1127 1022 979 694 687 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 205 237 849 881 1353 1385 2253 2285 4099
Size: 32 612 32 472 32 868 32 1814
Cuts at: 4099 4129 4131 4161 205
Size: 30 2 30 2301
Fragments arranged by size:
2301 1814 868 612 472 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 2362 2895 3296 2362
Size: 533 401 5323
Fragments arranged by size:
5323 533 401
HindIII A'AGCT_T
Cuts at: 1253 1253
Size: 6257
KasI G'GCGC_C
Cuts at: 3687 3935 3687
Size: 248 6009
KpnI G_GTAC'C
Cuts at: 845 3277 845
Size: 2432 3825
MfeI C'AATT_G
Cuts at: 2193 2193
Size: 6257
MluI A'CGCG_T
Cuts at: 5690 5690
Size: 6257
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4928 5112 4928
Size: 184 6073
MscI TGG'CCA
Cuts at: 4018 4018
Size: 6257
MslI CAynn'nnrTG
Cuts at: 1339 1589 1605 1919 3103 4246 4373 1339
Size: 250 16 314 1184 1143 127 3223
Fragments arranged by size:
3223 1184 1143 314 250 127 16
MspA1I CmG'CkG
Cuts at: 238 1008 1275 1753 1927 2122 3368 3682 4042
Size: 770 267 478 174 195 1246 314 360
Cuts at: 4042 5055 5300 238
Size: 1013 245 1195
Fragments arranged by size:
1246 1195 1013 770 478 360 314 267
245 195 174
NaeI GCC'GGC
Cuts at: 1317 4438 1317
Size: 3121 3136
NarI GG'CG_CC
Cuts at: 3688 3936 3688
Size: 248 6009
NdeI CA'TA_TG
Cuts at: 2945 2945
Size: 6257
NgoMIV G'CCGG_C
Cuts at: 1315 4436 1315
Size: 3121 3136
NsiI A_TGCA'T
Cuts at: 967 967
Size: 6257
NspI r_CATG'y
Cuts at: 135 312 358 588 633 3310 3810 4717 135
Size: 177 46 230 45 2677 500 907 1675
Fragments arranged by size:
2677 1675 907 500 230 177 46 45
PciI A'CATG_T
Cuts at: 354 584 4713 354
Size: 230 4129 1898
Fragments arranged by size:
4129 1898 230
PflMI CCAn_nnn'nTGG
Cuts at: 1275 2298 1275
Size: 1023 5234
PfoI T'CCnGG_A
Cuts at: 2790 2790
Size: 6257
PmeI GTTT'AAAC
Cuts at: 3256 3256
Size: 6257
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 441 473 5396 5428 441
Size: 32 4923 32 1270
Fragments arranged by size:
4923 1270 32 32
PshAI GACnn'nnGTC
Cuts at: 2868 2868
Size: 6257
PspXI vC'TCGA_Gb
Cuts at: 888 888
Size: 6257
PstI C_TGCA'G
Cuts at: 319 674 908 1632 2131 2835 3304 3989 319
Size: 355 234 724 499 704 469 685 2587
Fragments arranged by size:
2587 724 704 685 499 469 355 234
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6257
PvuII CAG'CTG
Cuts at: 1008 1275 1927 2122 3682 4042 1008
Size: 267 652 195 1560 360 3223
Fragments arranged by size:
3223 1560 652 360 267 195
RsrII CG'GwC_CG
Cuts at: 4452 4452
Size: 6257
SacI G_AGCT'C
Cuts at: 3031 3271 3031
Size: 240 6017
SalI G'TCGA_C
Cuts at: 2360 3294 2360
Size: 934 5323
SapI GCTCTTCn'nnn_
Cuts at: 2192 4280 4490 2192
Size: 2088 210 3959
Fragments arranged by size:
3959 2088 210
SbfI CC_TGCA'GG
Cuts at: 3304 3304
Size: 6257
ScaI AGT'ACT
Cuts at: 5774 5774
Size: 6257
SfcI C'TryA_G
Cuts at: 33 315 670 904 1628 2028 2127 2831 3300
Size: 282 355 234 724 400 99 704 469
Cuts at: 3300 3985 4978 5169 33
Size: 685 993 191 1121
Fragments arranged by size:
1121 993 724 704 685 469 400 355
282 234 191 99
SfoI GGC'GCC
Cuts at: 3689 3937 3689
Size: 248 6009
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6257
SmaI CCC'GGG
Cuts at: 2904 3279 2904
Size: 375 5882
SmlI C'TyrA_G
Cuts at: 888 918 1834 3412 4327 4819 5081 888
Size: 30 916 1578 915 492 262 2064
Fragments arranged by size:
2064 1578 916 915 492 262 30
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6257
SphI G_CATG'C
Cuts at: 3310 3810 3310
Size: 500 5757
SspI AAT'ATT
Cuts at: 1569 1569
Size: 6257
StuI AGG'CCT
Cuts at: 1136 3153 1136
Size: 2017 4240
StyI C'CwwG_G
Cuts at: 299 461 610 2036 2595 3016 3391 3745 299
Size: 162 149 1426 559 421 375 354 2811
Fragments arranged by size:
2811 1426 559 421 375 354 162 149
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2089 3840 3973 4432 4641 2089
Size: 1751 133 459 209 3705
Fragments arranged by size:
3705 1751 459 209 133
TatI w'GTAC_w
Cuts at: 155 5772 155
Size: 5617 640
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2384 2416 5774 5806 2384
Size: 32 3358 32 2835
Fragments arranged by size:
3358 2835 32 32
Tth111I GACn'n_nGTC
Cuts at: 4054 4054
Size: 6257
XbaI T'CTAG_A
Cuts at: 3288 3288
Size: 6257
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1338 2943 3752 1338
Size: 1605 809 3843
Fragments arranged by size:
3843 1605 809
XhoI C'TCGA_G
Cuts at: 888 888
Size: 6257
XmaI C'CCGG_G
Cuts at: 2902 3277 2902
Size: 375 5882
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NdeI NgoMIV NsiI
NspI PciI PflMI PfoI PmeI PpiI PshAI PspXI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI
Enzymes that do not cut:
AatII AhdI ApaI AscI AvrII BaeI BglI BmtI
BsiWI BsmBI BstEII BstZ17I Bsu36I ClaI CspCI DraIII
EcoRV FseI FspAI HpaI NcoI NheI NotI NruI
PacI PasI PmlI PpuMI PsiI PspOMI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SwaI XmnI
ZraI