Restriction map for
pF1KE2421
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# NO CUT ENZYMES ( 82 enzymes) #
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AatII AccII AclI AgeI AhdI
Aor51HI AscI AspEI AviII AvrII
BbeI BbrPI BcnI BlnI BsaAI
BsiEI BsiWI BsmBI BspDI BsrBI
BsrFI BssHII Bst1107I BstAPI BstEII
BstPI BstUI BstZ17I CfoI Cfr10I
ClaI CpoI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
FseI FspI HaeII HhaI HinP1I
HpaI KasI KspI MfeI MluI
MunI MvnI NaeI NarI NciI
NcoI NgoMIV NheI NotI NruI
PacI PaeR7I PinAI PmaCI PmeI
PmlI Psp1406I PvuI RsrII SacII
SalI SapI SbfI SfiI SfoI
SgrAI SmaI SnaBI Sse8387I XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 33 enzymes) #
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Acc65I 1811 GGTACC G^GTACC
AccI 1033 GTAGAC GT^[AC][GT]AC
AccIII 6223 TCCGGA T^CCGGA
AcyI 906 GGCGTC G[GA]^CG[CT]C
AflII 319 CTTAAG C^TTAAG
Asp718I 1811 GGTACC G^GTACC
AspI 2441 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BcgI 6393 GCAGACTTATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 319 CTTAAG C^TTAAG
BglI 6056 GCCTTATTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 906 GGCGTC G[GA]^CG[CT]C
BsaWI 6223 TCCGGA [AT]^CCGG[AT]
BseAI 6223 TCCGGA T^CCGGA
BspEI 6223 TCCGGA T^CCGGA
BstBI 6974 TTCGAA TT^CGAA
DsaI 5301 CCGTGG C^C[GA][CT]GG
EcoRV 3945 GATATC GAT^ATC
EcoT22I 3929 ATGCAT ATGCA^T
HapII 6224 CCGG C^CGG
Hin1I 906 GGCGTC G[GA]^CG[CT]C
HpaII 6224 CCGG C^CGG
KpnI 1811 GGTACC GGTAC^C
MroI 6223 TCCGGA T^CCGGA
MspI 6224 CCGG C^CGG
NsiI 3929 ATGCAT ATGCA^T
NspV 6974 TTCGAA TT^CGAA
PflFI 2441 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
ScaI 3795 AGTACT AGT^ACT
SfuI 6974 TTCGAA TT^CGAA
SpeI 585 ACTAGT A^CTAGT
SwaI 6566 ATTTAAAT ATTT^AAAT
Tth111I 2441 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 6945 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 30 enzymes) #
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AflIII 5202 ACATGT A^C[GA][CT]GT
5937 ACATGT
ApaI 3303 GGGCCC GGGCC^C
6194 GGGCCC
AseI 3117 ATTAAT AT^TAAT
3666 ATTAAT
AsnI 3117 ATTAAT AT^TAAT
3666 ATTAAT
AvaI 731 CTCGGG C^[CT]CG[GA]G
4101 CCCGAG
BamHI 1869 GGATCC G^GATCC
4743 GGATCC
BbvCI 1303 CCTCAGC CCTCAGC(-5/-2)
289 GCTGAGG
BclI 402 TGATCA T^GATCA
4419 TGATCA
BglII 1680 AGATCT A^GATCT
4210 AGATCT
BlpI 241 GCTAAGC GC^T[ACGT]AGC
5889 GCTCAGC
Bpu1102I 241 GCTAAGC GC^T[ACGT]AGC
5889 GCTCAGC
BsgI 80 GTGCAG GTGCAG(16/14)
1858 GTGCAG
BsoBI 731 CTCGGG C^[CT]CG[GA]G
4101 CCCGAG
BspHI 3195 TCATGA T^CATGA
3725 TCATGA
BspLU11I 5202 ACATGT A^CATGT
5937 ACATGT
BssSI 1370 CTCGTG CACGAG(-5/-1)
4615 CTCGTG
CelII 241 GCTAAGC GC^T[ACGT]AGC
5889 GCTCAGC
EcoNI 2608 CCTGAGTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4629 CCTTCTTCAGG
FbaI 402 TGATCA T^GATCA
4419 TGATCA
HgaI 136 GACGC GACGC(5/10)
907 GCGTC
MaeII 3536 ACGT A^CGT
3984 ACGT
PflMI 2515 CCATCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5714 CCAAAAGCTGG
PshAI 1036 GACAGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4269 GACCAGGGTC
PshBI 3117 ATTAAT AT^TAAT
3666 ATTAAT
PvuII 1038 CAGCTG CAG^CTG
1850 CAGCTG
RcaI 3195 TCATGA T^CATGA
3725 TCATGA
SacI 10 GAGCTC GAGCT^C
2987 GAGCTC
SexAI 545 ACCTGGT A^CC[AT]GGT
6661 ACCTGGT
SphI 3931 GCATGC GCATG^C
6581 GCATGC
Van91I 2515 CCATCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5714 CCAAAAGCTGG
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# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AciI AcsI AfaI AluI Alw44I
AlwI AlwNI AocI ApaLI ApoI
Asp700I AspHI AvaII BalI BanI
BanII BbsI BbvI BciVI BfaI
BmyI BpmI BpuAI BsaBI BsaI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I Bsp1407I
BspMI BsrDI BsrGI BsrI BssKI
BstF5I BstNI BstXI BstYI Bsu36I
Cac8I Cfr13I DdeI DpnII DraI
DraII EaeI EarI Eco81I EcoO109I
EcoRI EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HincII HindII HindIII
HinfI HphI ItaI Ksp632I MaeI
MaeIII MamI MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MvaI MwoI NdeI NdeII
NlaIII NlaIV NspI PleI PpuMI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspBI SspI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII XmnI