Restriction map for pF1KE6673
 (Linear) (Six-base) MAPSORT of: KIEE6673.seq  Check: 2893  from: 1  to: 927



KIEE6673.orf

 With 182 enzymes: SgfI * 

                        July 16, 2019 14:21  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     345     351     927 
   Size:        345       6     576

  Fragments arranged by size: 

                 576     345       6
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     181     927 
   Size:        181     746
 
ApaI G_GGCC'C

Cuts at:      0      28     927 
   Size:         28     899
 
ApoI r'AATT_y

Cuts at:      0     456     927 
   Size:        456     471
 
AvaI C'yCGr_G

Cuts at:      0     670     852     876     927 
   Size:        670     182      24      51

  Fragments arranged by size: 

                 670     182      51      24
 
BanI G'GyrC_C

Cuts at:      0     245     511     760     856     927 
   Size:        245     266     249      96      71

  Fragments arranged by size: 

                 266     249     245      96      71
 
BanII G_rGCy'C

Cuts at:      0       7      28     101     211     849     927 
   Size:          7      21      73     110     638      78

  Fragments arranged by size: 

                 638     110      78      73      21       7
 
BbeI G_GCGC'C

Cuts at:      0     764     927 
   Size:        764     163
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     774     927 
   Size:        774     153
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      93     127     927 
   Size:         93      34     800

  Fragments arranged by size: 

                 800      93      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     536     656     827     844     927 
   Size:        536     120     171      17      83

  Fragments arranged by size: 

                 536     171     120      83      17
 
Bme1580I G_kGCm'C

Cuts at:      0      28     514     634     859     927 
   Size:         28     486     120     225      68

  Fragments arranged by size: 

                 486     225     120      68      28
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     607     927 
   Size:        607     320
 
BsaHI Gr'CG_yC

Cuts at:      0     428     761     927 
   Size:        428     333     166

  Fragments arranged by size: 

                 428     333     166
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     712     742     927 
   Size:        712      30     185

  Fragments arranged by size: 

                 712     185      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     717     927 
   Size:        717     210
 
BseYI C'CCAG_C

Cuts at:      0     382     702     927 
   Size:        382     320     225

  Fragments arranged by size: 

                 382     320     225
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     379     927 
   Size:        379     548
 
BsiEI CG_ry'CG

Cuts at:      0      47     927 
   Size:         47     880
 
BsiHKAI G_wGCw'C

Cuts at:      0       7     101     927 
   Size:          7      94     826

  Fragments arranged by size: 

                 826      94       7
 
BsiWI C'GTAC_G

Cuts at:      0      32     927 
   Size:         32     895
 
Bsp1286I G_dGCh'C

Cuts at:      0       7      28     101     211     514     634     849     859 
   Size:          7      21      73     110     303     120     215      10

Cuts at:    859     927 
   Size:         68

  Fragments arranged by size: 

                 303     215     120     110      73      68      21      10
                   7
 
BspHI T'CATG_A

Cuts at:      0     188     927 
   Size:        188     739
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     345     351     927 
   Size:        345       6     576

  Fragments arranged by size: 

                 576     345       6
 
BsrBI CCG'CTC

Cuts at:      0     230     413     453     773     927 
   Size:        230     183      40     320     154

  Fragments arranged by size: 

                 320     230     183     154      40
 
BsrFI r'CCGG_y

Cuts at:      0      59     124     794     859     919     927 
   Size:         59      65     670      65      60       8

  Fragments arranged by size: 

                 670      65      65      60      59       8
 
BssSI C'ACGA_G

Cuts at:      0     555     927 
   Size:        555     372
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     654     927 
   Size:        654     273
 
BtgI C'CryG_G

Cuts at:      0     281     599     927 
   Size:        281     318     328

  Fragments arranged by size: 

                 328     318     281
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     427     927 
   Size:        427     500
 
EaeI y'GGCC_r

Cuts at:      0      44     483     774     836     927 
   Size:         44     439     291      62      91

  Fragments arranged by size: 

                 439     291      91      62      44
 
EagI C'GGCC_G

Cuts at:      0      44     927 
   Size:         44     883
 
EarI CTCTTCn'nnn_

Cuts at:      0     374     927 
   Size:        374     553
 
EcoICRI GAG'CTC

Cuts at:      0       5      99     927 
   Size:          5      94     828

  Fragments arranged by size: 

                 828      94       5
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     181     607     927 
   Size:        181     426     320

  Fragments arranged by size: 

                 426     320     181
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     316     927 
   Size:        316     611
 
EcoO109I rG'GnC_Cy

Cuts at:      0      24      25     156     674     885     927 
   Size:         24       1     131     518     211      42

  Fragments arranged by size: 

                 518     211     131      42      24       1
 
HaeII r_GCGC'y

Cuts at:      0     764     927 
   Size:        764     163
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     549     581     712     744     927 
   Size:        549      32     131      32     183

  Fragments arranged by size: 

                 549     183     131      32      32
 
KasI G'GCGC_C

Cuts at:      0     760     927 
   Size:        760     167
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     290     927 
   Size:        290     637
 
MscI TGG'CCA

Cuts at:      0     485     927 
   Size:        485     442
 
MslI CAynn'nnrTG

Cuts at:      0     187     569     689     927 
   Size:        187     382     120     238

  Fragments arranged by size: 

                 382     238     187     120
 
NaeI GCC'GGC

Cuts at:      0     796     921     927 
   Size:        796     125       6

  Fragments arranged by size: 

                 796     125       6
 
NarI GG'CG_CC

Cuts at:      0     761     927 
   Size:        761     166
 
NcoI C'CATG_G

Cuts at:      0     281     599     927 
   Size:        281     318     328

  Fragments arranged by size: 

                 328     318     281
 
NgoMIV G'CCGG_C

Cuts at:      0     794     919     927 
   Size:        794     125       8

  Fragments arranged by size: 

                 794     125       8
 
PpuMI rG'GwC_Cy

Cuts at:      0     674     885     927 
   Size:        674     211      42

  Fragments arranged by size: 

                 674     211      42
 
PspOMI G'GGCC_C

Cuts at:      0      24     927 
   Size:         24     903
 
PstI C_TGCA'G

Cuts at:      0     410     506     743     927 
   Size:        410      96     237     184

  Fragments arranged by size: 

                 410     237     184      96
 
SacI G_AGCT'C

Cuts at:      0       7     101     927 
   Size:          7      94     826

  Fragments arranged by size: 

                 826      94       7
 
SanDI GG'GwC_CC

Cuts at:      0     674     927 
   Size:        674     253
 
ScaI AGT'ACT

Cuts at:      0     436     817     927 
   Size:        436     381     110

  Fragments arranged by size: 

                 436     381     110
 
SfcI C'TryA_G

Cuts at:      0     199     406     502     739     927 
   Size:        199     207      96     237     188

  Fragments arranged by size: 

                 237     207     199     188      96
 
SfoI GGC'GCC

Cuts at:      0     762     927 
   Size:        762     165
 
SgrAI Cr'CCGG_yG

Cuts at:      0     859     927 
   Size:        859      68
 
SmaI CCC'GGG

Cuts at:      0     672     878     927 
   Size:        672     206      49

  Fragments arranged by size: 

                 672     206      49
 
StyI C'CwwG_G

Cuts at:      0     281     599     927 
   Size:        281     318     328

  Fragments arranged by size: 

                 328     318     281
 
TatI w'GTAC_w

Cuts at:      0     434     815     927 
   Size:        434     381     112

  Fragments arranged by size: 

                 434     381     112
 
XmaI C'CCGG_G

Cuts at:      0     670     876     927 
   Size:        670     206      51

  Fragments arranged by size: 

                 670     206      51
 

 Enzymes that do cut:

     AarI     AcuI     ApaI     ApoI     AvaI     BanI    BanII     BbeI
     BbsI     BcgI     BglI Bme1580I     BpmI    BsaHI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI Bsp1286I    BspHI    BspMI
    BsrBI    BsrFI    BssSI    BstXI     BtgI     DrdI     EaeI     EagI
     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I    HaeII    Hin4I     KasI
     MmeI     MscI     MslI     NaeI     NarI     NcoI   NgoMIV    PpuMI
   PspOMI     PstI     SacI    SanDI     ScaI     SfcI     SfoI    SgrAI
     SmaI     StyI     TatI     XmaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI    BbvCI    BciVI     BclI    BfrBI
    BglII     BlpI    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaWI     BsmI    BsmBI    BspEI    BsrDI
    BsrGI   BssHII   BstAPI    BstBI   BstEII    BstYI  BstZ17I   Bsu36I
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     EciI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI   HincII  HindIII     HpaI
     KpnI     MfeI     MluI   MspA1I     NdeI     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI    PvuII
    RsrII    SacII     SalI     SapI     SbfI    SexAI     SfiI     SgfI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI     XmnI     ZraI
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