Restriction map for
pF1KB7344
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# NO CUT ENZYMES ( 101 enzymes) #
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AatII Acc65I AccII AccIII AclI
AflII AflIII AgeI AhdI Aor51HI
ApaI AscI Asp718I AspEI AviII
BamHI BbeI BbrPI BbvCI BcgI
BfrI BlpI Bpu1102I BsaAI BsaI
BsaWI BseAI BsiEI BsiWI BsmBI
BspDI BspEI BspHI BspLU11I BsrFI
BssHII BssSI Bst1107I BstEII BstPI
BstUI BstZ17I CelII Cfr10I ClaI
CpoI DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoRV FauI FseI FspI HindIII
KasI KpnI KspI MfeI MluI
MroI MunI MvnI NaeI NarI
NcoI NgoMIV NotI NruI PacI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PstI PvuI RcaI RsrII
SacII SalI SapI SbfI SfiI
SfoI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SwaI XcmI XmaCI
XmaI
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# ONE CUT ENZYMES ( 62 enzymes) #
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AcyI 2132 GACGCC G[GA]^CG[CT]C
Alw44I 2551 GTGCAC G^TGCAC
AlwNI 1027 CAGAGTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 2356 CCTCAGG CC^T[ACGT]AGG
ApaLI 2551 GTGCAC G^TGCAC
Asp700I 2030 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 2511 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 2286 CCTAGG C^CTAGG
BciVI 2244 GGATAC GTATCC(6/5)
BclI 276 TGATCA T^GATCA
BcnI 1594 CCCGG CC^[GC]GG
BglI 2933 GCCTGGTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1929 AGATCT A^GATCT
BlnI 2286 CCTAGG C^CTAGG
BsaBI 1402 GATGATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 2132 GACGCC G[GA]^CG[CT]C
BsgI 1736 GTGCAG GTGCAG(16/14)
Bsp1407I 1629 TGTACA T^GTACA
BspMI 1292 ACCTGC ACCTGC(4/8)
BsrBI 2961 GAGCGG CCGCTC(-3/-3)
BsrDI 28 GCAATG GCAATG(2/0)
BsrGI 1629 TGTACA T^GTACA
BstAPI 1026 GCAGAGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 653 TTCGAA TT^CGAA
BstXI 3072 CCAAACCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 2356 CCTCAGG CC^T[ACGT]AGG
CfoI 2621 GCGC GCG^C
DraI 990 TTTAAA TTT^AAA
EarI 889 GAAGAG CTCTTC(1/4)
Eco81I 2356 CCTCAGG CC^T[ACGT]AGG
EcoNI 2352 CCTTCCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 70 GAATTC G^AATTC
EcoT22I 1875 ATGCAT ATGCA^T
FbaI 276 TGATCA T^GATCA
HaeII 2620 GGCGCT [GA]GCGC^[CT]
HhaI 2621 GCGC GCG^C
Hin1I 2132 GACGCC G[GA]^CG[CT]C
HinP1I 2621 GCGC G^CGC
HincII 61 GTTAAC GT[CT]^[GA]AC
HindII 61 GTTAAC GT[CT]^[GA]AC
HpaI 61 GTTAAC GTT^AAC
Ksp632I 889 GAAGAG CTCTTC(1/4)
MamI 1402 GATGATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 2943 CACCAGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NciI 1594 CCCGG CC^[GC]GG
NheI 1296 GCTAGC G^CTAGC
NsiI 1875 ATGCAT ATGCA^T
NspI 743 GCATGT [GA]CATG^[CT]
NspV 653 TTCGAA TT^CGAA
PaeR7I 862 CTCGAG C^TCGAG
PflFI 2511 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
ScaI 1525 AGTACT AGT^ACT
SexAI 2451 ACCTGGT A^CC[AT]GGT
SfcI 1397 CTATAG C^T[GA][CT]AG
SfuI 653 TTCGAA TT^CGAA
SmlI 862 CTCGAG C^T[CT][GA]AG
SspBI 1629 TGTACA T^GTACA
SspI 1438 AATATT AAT^ATT
StuI 415 AGGCCT AGG^CCT
Tth111I 2511 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 862 CTCGAG C^TCGAG
XmnI 2030 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 18 enzymes) #
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AccI 1244 GTAGAC GT^[AC][GT]AC
2711 GTCTAC
BalI 2323 TGGCCA TGG^CCA
3036 TGGCCA
BsmAI 1214 GAGAC GTCTC(1/5)
1086 GTCTC
BstYI 293 GGATCT [GA]^GATC[CT]
1929 AGATCT
DraII 2253 GGGACCT [GA]G^G[ACGT]CC[CT]
2448 GGGACCT
EcoO109I 2253 GGGACCT [GA]G^G[ACGT]CC[CT]
2448 GGGACCT
HapII 1595 CCGG C^CGG
2792 CCGG
HgaI 547 GACGC GACGC(5/10)
2132 GACGC
HpaII 1595 CCGG C^CGG
2792 CCGG
MflI 293 GGATCT [GA]^GATC[CT]
1929 AGATCT
MluNI 2323 TGGCCA TGG^CCA
3036 TGGCCA
MscI 2323 TGGCCA TGG^CCA
3036 TGGCCA
MspI 1595 CCGG C^CGG
2792 CCGG
PpuMI 2253 GGGACCT [GA]G^G[AT]CC[CT]
2448 GGGACCT
PvuII 1286 CAGCTG CAG^CTG
1739 CAGCTG
SacI 383 GAGCTC GAGCT^C
424 GAGCTC
XbaI 420 TCTAGA T^CTAGA
672 TCTAGA
XhoII 293 GGATCT [GA]^GATC[CT]
1929 AGATCT
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# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AseI AsnI AspHI AvaI
AvaII BanI BanII BbsI BbvI
BfaI BmyI BpmI BpuAI BsaJI
BseRI BsiHKAI BsiYI BslI BsmFI
BsmI BsoBI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII EaeI EcoRII EcoT14I Fnu4HI
FokI HaeIII HinfI HphI ItaI
MaeI MaeII MaeIII MboI MboII
MnlI MseI MspA1I MvaI MwoI
NdeI NdeII NlaIII NlaIV PflMI
PleI PshBI RsaI Sau3AI Sau96I
ScrFI SfaNI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I