Restriction map for pF1KE1686
##################################
# NO CUT ENZYMES (  177 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwNI       AocI        
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AvaI        AviII       BalI        BamHI       
BanI        BbeI        BbrPI       BbvCI       BcgI        
BciVI       BclI        BfrI        BglII       BlpI        
BpmI        Bpu1102I    BsaAI       BsaBI       BsaHI       
BsaWI       BseAI       BseRI       BsgI        BsiEI       
BsiWI       BsmBI       BsoBI       Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrDI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstBI       BstEII      BstPI       BstUI       
BstYI       BstZ17I     Bsu36I      CelII       CfoI        
Cfr10I      ClaI        CpoI        DraI        DraIII      
DrdI        DsaI        EaeI        EagI        Eam1105I    
EarI        EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO65I     EcoRI       EcoRV       EcoT22I     
FbaI        FseI        FspI        HaeII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HindIII     HpaI        KasI        KpnI        Ksp632I     
KspI        MaeII       MamI        MfeI        MflI        
MluI        MluNI       MroI        MscI        MslI        
MspA1I      MunI        MvnI        NaeI        NarI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
PvuII       RcaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        SexAI       SfaNI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        Sse8387I    SspBI       
SspI        SwaI        TfiI        Tth111I     Van91I      
XbaI        XcmI        XhoI        XhoII       XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AfaI          453  GTAC                 GT^AC
AspHI         393  GAGCAC               G[AT]GC[AT]^C
AvaII         105  GGTCC                G^G[AT]CC
AvrII           6  CCTAGG               C^CTAGG
BanII          41  GGGCTC               G[GA]GC[CT]^C
BbvI          114  GCAGC                GCAGC(8/12)
BcnI          325  CCCGG                CC^[GC]GG
BglI          320  GCCAGCCCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI            6  CCTAGG               C^CTAGG
BsaI           10  GGTCTC               GGTCTC(1/5)
BsiHKAI       393  GAGCAC               G[AT]GC[AT]^C
BsmAI          11  GTCTC                GTCTC(1/5)
BsmI          174  GAATGC               GAATGC(1/-1)
BstAPI        169  GCAGGGAATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I        516  CATCC                GGATG(2/0)
BstXI         527  CCAAATCTCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII         104  AGGTCCC              [GA]G^G[ACGT]CC[CT]
EcoO109I      104  AGGTCCC              [GA]G^G[ACGT]CC[CT]
FauI          201  CCCGC                CCCGC(4/6)
Fnu4HI        114  GCAGC                GC^[ACGT]GC
FokI          516  CATCC                GGATG(9/13)
HapII         326  CCGG                 C^CGG
HinfI          64  GACTC                G^A[ACGT]TC
HpaII         326  CCGG                 C^CGG
ItaI          114  GCAGC                GC^[ACGT]GC
MspI          326  CCGG                 C^CGG
NciI          325  CCCGG                CC^[GC]GG
NlaIV         105  GGTCCC               GG[ACGT]^[ACGT]CC
PleI           64  GACTC                GAGTC(4/5)
PpuMI         104  AGGTCCC              [GA]G^G[AT]CC[CT]
PstI          112  CTGCAG               CTGCA^G
RsaI          453  GTAC                 GT^AC
ScaI          452  AGTACT               AGT^ACT
SfcI          112  CTGCAG               C^T[GA][CT]AG
StuI          162  AGGCCT               AGG^CCT
TseI          114  GCAGC                G^C[AT]GC
TspRI         580  CAGTG                CA[GC]TG[ACGT][ACGT]^


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AciI          202  CCGC                 CCGC(-3/-1)
              519  CCGC                
AlwI           81  GATCC                GGATC(4/5)
              296  GGATC               
BfaI            7  CTAG                 C^TAG
               28  CTAG                
BmyI           41  GGGCTC               G[AGT]GC[ACT]^C
              393  GAGCAC              
BsmFI         106  GTCCC                GGGAC(10/14)
              568  GTCCC               
Bsp1286I       41  GGGCTC               G[AGT]GC[ACT]^C
              393  GAGCAC              
BsrI          293  ACTGG                ACTGG(1/-1)
              579  CCAGT               
EcoT14I         6  CCTAGG               C^C[AT][AT]GG
              482  CCAAGG              
MaeI            7  CTAG                 C^TAG
               28  CTAG                
MseI          350  TTAA                 T^TAA
              367  TTAA                
NlaIII         49  CATG                 CATG^
              412  CATG                
NspI           48  GCATGC               [GA]CATG^[CT]
              411  GCATGC              
SphI           48  GCATGC               GCATG^C
              411  GCATGC              
StyI            6  CCTAGG               C^C[AT][AT]GG
              482  CCAAGG              
TaqI          575  TCGA                 T^CGA
              587  TCGA                
Tru9I         350  TTAA                 T^TAA
              367  TTAA                
Tsp45I        256  GTCAC                ^GT[GC]AC
              287  GTGAC               
Tsp509I       137  AATT                 ^AATT
              180  AATT                
TthHB8I       575  TCGA                 T^CGA
              587  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   25 enzymes) #
#########################################

AluI        BbsI        BpuAI       BsaJI       BsiYI       
BslI        BssKI       BstNI       Cac8I       Cfr13I      
DdeI        DpnII       EcoRII      HaeIII      HphI        
MaeIII      MboI        MboII       MnlI        MvaI        
MwoI        NdeII       Sau3AI      Sau96I      ScrFI       
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