Restriction map for pF1KB6718
##################################
# NO CUT ENZYMES (  204 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AflII       AgeI        
AhdI        AlwI        AlwNI       AocI        Aor51HI     
ApaI        AscI        AseI        AsnI        Asp718I     
AspEI       AspI        AvaI        AvaII       AviII       
AvrII       BalI        BamHI       BanI        BanII       
BbeI        BbrPI       BbsI        BbvCI       BcgI        
BciVI       BclI        BcnI        BfrI        BglI        
BglII       BlnI        BlpI        Bpu1102I    BpuAI       
BsaBI       BsaHI       BsaWI       BseAI       BseRI       
BsgI        BsiEI       BsiWI       BsiYI       BslI        
BsmBI       BsmFI       BsmI        BsoBI       BspDI       
BspEI       BspHI       BspLU11I    BsrBI       BsrDI       
BsrFI       BssHII      BssKI       BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstNI       BstPI       
BstUI       BstXI       BstYI       BstZ17I     Bsu36I      
CelII       CfoI        Cfr10I      Cfr13I      ClaI        
CpoI        DpnII       DraI        DraII       DrdI        
DsaI        EaeI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRII      EcoRV       
EcoT22I     FauI        FbaI        FseI        FspI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HindIII     
HpaI        HpaII       KasI        KpnI        Ksp632I     
KspI        MaeIII      MamI        MboI        MfeI        
MflI        MluI        MluNI       MroI        MscI        
MslI        MspA1I      MspI        MunI        MvaI        
MvnI        NaeI        NarI        NciI        NcoI        
NdeI        NdeII       NgoMIV      NlaIII      NlaIV       
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RcaI        RsrII       SacI        SacII       SalI        
SapI        Sau3AI      Sau96I      SbfI        ScaI        
ScrFI       SexAI       SfcI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspI        StuI        
SwaI        Tsp45I      Tth111I     Van91I      XbaI        
XhoI        XhoII       XmaCI       XmaI        


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# ONE CUT ENZYMES (   39 enzymes) #
###################################

AcsI           68  AAATTC               [GA]^AATT[CT]
AflIII         47  ACGTGT               A^C[GA][CT]GT
Alw44I        186  GTGCAC               G^TGCAC
ApaLI         186  GTGCAC               G^TGCAC
ApoI           68  AAATTC               [GA]^AATT[CT]
Asp700I       196  GAAATCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI         186  GTGCAC               G[AT]GC[AT]^C
BbvI           40  GCTGC                GCAGC(8/12)
BmyI          186  GTGCAC               G[AGT]GC[ACT]^C
BpmI          220  CTGGAG               CTGGAG(16/14)
BsaAI          46  TACGTG               [CT]AC^GT[GA]
BsaI           94  GAGACC               GGTCTC(1/5)
BsaJI          98  CCTTGG               C^C[ACGT][ACGT]GG
BsiHKAI       186  GTGCAC               G[AT]GC[AT]^C
Bsp1286I      186  GTGCAC               G[AGT]GC[ACT]^C
Bsp1407I      165  TGTACA               T^GTACA
BspMI         353  ACCTGC               ACCTGC(4/8)
BsrGI         165  TGTACA               T^GTACA
BsrI          219  ACTGG                ACTGG(1/-1)
Cac8I          27  GCTAGC               GC[ACGT]^[ACGT]GC
DdeI          148  CTGAG                C^T[ACGT]AG
DraIII        381  CACCGAGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoT14I        98  CCTTGG               C^C[AT][AT]GG
Fnu4HI         40  GCTGC                GC^[ACGT]GC
ItaI           40  GCTGC                GC^[ACGT]GC
MaeII          47  ACGT                 A^CGT
MseI          277  TTAA                 T^TAA
MwoI           31  GCTCTTGGAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NheI           27  GCTAGC               G^CTAGC
SfaNI           6  GATGC                GCATC(5/9)
SspBI         165  TGTACA               T^GTACA
StyI           98  CCTTGG               C^C[AT][AT]GG
TaqI          120  TCGA                 T^CGA
TfiI          153  GATTC                G^A[AT]TC
Tru9I         277  TTAA                 T^TAA
TseI           40  GCTGC                G^C[AT]GC
TthHB8I       120  TCGA                 T^CGA
XcmI           74  CCACAAGTGCATTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI          196  GAAATCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   13 enzymes) #
###################################

AfaI          166  GTAC                 GT^AC
              245  GTAC                
AluI           30  AGCT                 AG^CT
               39  AGCT                
BfaI           28  CTAG                 C^TAG
              346  CTAG                
BsmAI          94  GAGAC                GTCTC(1/5)
              142  GAGAC               
BstF5I         57  CATCC                GGATG(2/0)
                5  GGATG               
FokI           57  CATCC                GGATG(9/13)
                5  GGATG               
HphI           87  GGTGA                GGTGA(8/7)
              177  TCACC               
MaeI           28  CTAG                 C^TAG
              346  CTAG                
MboII         193  GAAGA                GAAGA(8/7)
              319  GAAGA               
MnlI            3  GAGG                 CCTC(7/6)
              150  GAGG                
PleI          144  GACTC                GAGTC(4/5)
              225  GAGTC               
RsaI          166  GTAC                 GT^AC
              245  GTAC                
Tsp509I        69  AATT                 ^AATT
              341  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (    2 enzymes) #
#########################################

HinfI       TspRI       
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