################################## # NO CUT ENZYMES ( 106 enzymes) # ################################## Acc65I AccI AccIII AclI AcsI AflII AgeI AhdI Aor51HI ApoI AscI Asp718I AspEI AspI AviII BamHI BbrPI BbvCI BciVI BclI BfrI BglI BglII BlpI Bpu1102I BsaAI BsaI BseAI BsiWI BspDI BspEI BspLU11I BsrBI BsrDI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I CelII ClaI CpoI DrdI Eam1105I Eco47III EcoNI EcoO65I EcoRI EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KpnI KspI MfeI MluI MroI MunI NcoI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RsrII SacII SalI SapI SbfI ScaI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I StuI SwaI TfiI Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AatII 175 GACGTC GACGT^C AflIII 1124 ACGTGT A^C[GA][CT]GT Alw44I 270 GTGCAC G^TGCAC AlwNI 262 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 742 CCTGAGG CC^T[ACGT]AGG ApaI 1587 GGGCCC GGGCC^C ApaLI 270 GTGCAC G^TGCAC AseI 546 ATTAAT AT^TAAT AsnI 546 ATTAAT AT^TAAT Asp700I 460 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvrII 162 CCTAGG C^CTAGG BalI 1079 TGGCCA TGG^CCA BbsI 142 GTCTTC GAAGAC(2/6) BcgI 597 CGAAGAAGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfaI 163 CTAG C^TAG BlnI 162 CCTAGG C^CTAGG BpuAI 142 GTCTTC GAAGAC(2/6) BsaBI 1426 GATATTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 452 ACCGGA [AT]^CCGG[AT] BsgI 1544 CTGCAC GTGCAG(16/14) BsmAI 1362 GTCTC GTCTC(1/5) BsmBI 1361 CGTCTC CGTCTC(1/5) BsmI 460 GAATGC GAATGC(1/-1) Bsp1407I 541 TGTACA T^GTACA BspHI 1070 TCATGA T^CATGA BspMI 406 GCAGGT ACCTGC(4/8) BsrGI 541 TGTACA T^GTACA BstYI 311 AGATCC [GA]^GATC[CT] Bsu36I 742 CCTGAGG CC^T[ACGT]AGG DraI 849 TTTAAA TTT^AAA DraIII 569 CACGATGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 186 CCACGG C^C[GA][CT]GG Eco81I 742 CCTGAGG CC^T[ACGT]AGG EcoRV 500 GATATC GAT^ATC MaeI 163 CTAG C^TAG MamI 1426 GATATTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 311 AGATCC [GA]^GATC[CT] MluNI 1079 TGGCCA TGG^CCA MscI 1079 TGGCCA TGG^CCA PflMI 1333 CCAGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshBI 546 ATTAAT AT^TAAT PstI 842 CTGCAG CTGCA^G RcaI 1070 TCATGA T^CATGA SacI 1036 GAGCTC GAGCT^C SexAI 137 ACCTGGT A^CC[AT]GGT SspBI 541 TGTACA T^GTACA Van91I 1333 CCAGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 311 AGATCC [GA]^GATC[CT] XmnI 460 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AlwI 312 GATCC GGATC(4/5) 1564 GATCC AvaI 29 CCCGAG C^[CT]CG[GA]G 108 CTCGGG BbeI 96 GGCGCC GGCGC^C 112 GGCGCC BpmI 712 CTCCAG CTGGAG(16/14) 1232 CTCCAG BseRI 55 CTCCTC GAGGAG(10/8) 1318 CTCCTC BsiEI 127 CGGCCG CG[GA][CT]^CG 227 CGGCCG BsoBI 29 CCCGAG C^[CT]CG[GA]G 108 CTCGGG BsrFI 125 GCCGGC [GA]^CCGG[CT] 229 GCCGGC Cfr10I 125 GCCGGC [GA]^CCGG[CT] 229 GCCGGC EagI 127 CGGCCG C^GGCCG 227 CGGCCG EclXI 127 CGGCCG C^GGCCG 227 CGGCCG Eco52I 127 CGGCCG C^GGCCG 227 CGGCCG HaeII 96 GGCGCC [GA]GCGC^[CT] 112 GGCGCC HinfI 1201 GACTC G^A[ACGT]TC 1246 GACTC HphI 644 TCACC GGTGA(8/7) 1207 TCACC KasI 96 GGCGCC G^GCGCC 112 GGCGCC MaeII 176 ACGT A^CGT 1124 ACGT NaeI 125 GCCGGC GCC^GGC 229 GCCGGC NarI 96 GGCGCC GG^CGCC 112 GGCGCC NdeI 421 CATATG CA^TATG 761 CATATG NgoMIV 125 GCCGGC G^CCGGC 229 GCCGGC PleI 1201 GACTC GAGTC(4/5) 1246 GACTC PvuII 289 CAGCTG CAG^CTG 357 CAGCTG SfaNI 197 GCATC GCATC(5/9) 789 GCATC SfoI 96 GGCGCC GGC^GCC 112 GGCGCC SspI 1180 AATATT AAT^ATT 1262 AATATT XcmI 288 CCAGCTGCTCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1217 CCAGGAACAGCATGG ######################################### # 3 OR MORE CUT ENZYMES ( 76 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AspHI AvaII BanI BanII BbvI BcnI BmyI BsaHI BsaJI BsiHKAI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HpaII ItaI Ksp632I MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PpuMI RsaI Sau3AI Sau96I ScrFI SfcI StyI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I