(Linear) (Six-base) MAPSORT of: KIEE2430.seq Check: 8487 from: 1 to: 1599 KIEE2430.orf With 182 enzymes: SgfI * January 7, 2013 14:38 .. AatII G_ACGT'C Cuts at: 0 179 1599 Size: 179 1420 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 718 1030 1463 1599 Size: 718 312 433 136 Fragments arranged by size: 718 433 312 136 AflIII A'CryG_T Cuts at: 0 1124 1599 Size: 1124 475 AleI CACnn'nnGTG Cuts at: 0 1579 1599 Size: 1579 20 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 812 1599 Size: 812 787 AlwNI CAG_nnn'CTG Cuts at: 0 267 1599 Size: 267 1332 ApaI G_GGCC'C Cuts at: 0 1591 1599 Size: 1591 8 ApaLI G'TGCA_C Cuts at: 0 270 1599 Size: 270 1329 AseI AT'TA_AT Cuts at: 0 547 1599 Size: 547 1052 AvaI C'yCGr_G Cuts at: 0 29 108 1599 Size: 29 79 1491 Fragments arranged by size: 1491 79 29 AvrII C'CTAG_G Cuts at: 0 162 1599 Size: 162 1437 BanI G'GyrC_C Cuts at: 0 96 112 437 1599 Size: 96 16 325 1162 Fragments arranged by size: 1162 325 96 16 BanII G_rGCy'C Cuts at: 0 109 731 866 1040 1591 1599 Size: 109 622 135 174 551 8 Fragments arranged by size: 622 551 174 135 109 8 BbeI G_GCGC'C Cuts at: 0 100 116 1599 Size: 100 16 1483 Fragments arranged by size: 1483 100 16 BbsI GAAGACnn'nnnn_ Cuts at: 0 135 1599 Size: 135 1464 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 586 620 1599 Size: 586 34 979 Fragments arranged by size: 979 586 34 Bme1580I G_kGCm'C Cuts at: 0 274 1591 1599 Size: 274 1317 8 Fragments arranged by size: 1317 274 8 BmgBI CAC'GTC Cuts at: 0 1125 1599 Size: 1125 474 BmrI ACTGGGnnnn_n' Cuts at: 0 1586 1599 Size: 1586 13 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1404 1599 Size: 1404 195 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 697 1217 1599 Size: 697 520 382 Fragments arranged by size: 697 520 382 BsaBI GATnn'nnATC Cuts at: 0 1430 1599 Size: 1430 169 BsaHI Gr'CG_yC Cuts at: 0 97 113 176 974 1599 Size: 97 16 63 798 625 Fragments arranged by size: 798 625 97 63 16 BsaWI w'CCGG_w Cuts at: 0 452 1599 Size: 452 1147 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 46 1309 1599 Size: 46 1263 290 Fragments arranged by size: 1263 290 46 BseYI C'CCAG_C Cuts at: 0 355 389 1599 Size: 355 34 1210 Fragments arranged by size: 1210 355 34 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1529 1599 Size: 1529 70 BsiEI CG_ry'CG Cuts at: 0 130 230 1599 Size: 130 100 1369 Fragments arranged by size: 1369 130 100 BsiHKAI G_wGCw'C Cuts at: 0 242 274 579 1040 1599 Size: 242 32 305 461 559 Fragments arranged by size: 559 461 305 242 32 BsmI GAATG_Cn' Cuts at: 0 466 1599 Size: 466 1133 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1367 1599 Size: 1367 232 Bsp1286I G_dGCh'C Cuts at: 0 109 242 274 579 731 866 1040 1591 Size: 109 133 32 305 152 135 174 551 Cuts at: 1591 1599 Size: 8 Fragments arranged by size: 551 305 174 152 135 133 109 32 8 BspHI T'CATG_A Cuts at: 0 1070 1599 Size: 1070 529 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 397 1599 Size: 397 1202 BsrFI r'CCGG_y Cuts at: 0 125 229 1599 Size: 125 104 1370 Fragments arranged by size: 1370 125 104 BsrGI T'GTAC_A Cuts at: 0 541 1599 Size: 541 1058 BstYI r'GATC_y Cuts at: 0 311 1599 Size: 311 1288 Bsu36I CC'TnA_GG Cuts at: 0 743 1599 Size: 743 856 BtgI C'CryG_G Cuts at: 0 186 1599 Size: 186 1413 BtsI GCAGTG_nn' Cuts at: 0 839 864 1599 Size: 839 25 735 Fragments arranged by size: 839 735 25 DraI TTT'AAA Cuts at: 0 851 1599 Size: 851 748 DraIII CAC_nnn'GTG Cuts at: 0 574 1599 Size: 574 1025 EaeI y'GGCC_r Cuts at: 0 127 227 300 1079 1599 Size: 127 100 73 779 520 Fragments arranged by size: 779 520 127 100 73 EagI C'GGCC_G Cuts at: 0 127 227 1599 Size: 127 100 1372 Fragments arranged by size: 1372 127 100 EarI CTCTTCn'nnn_ Cuts at: 0 1209 1254 1259 1599 Size: 1209 45 5 340 Fragments arranged by size: 1209 340 45 5 EcoICRI GAG'CTC Cuts at: 0 1038 1599 Size: 1038 561 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 697 718 1030 1217 1463 1599 Size: 697 21 312 187 246 136 Fragments arranged by size: 697 312 246 187 136 21 EcoO109I rG'GnC_Cy Cuts at: 0 34 738 1437 1551 1587 1588 1599 Size: 34 704 699 114 36 1 11 Fragments arranged by size: 704 699 114 36 34 11 1 EcoRV GAT'ATC Cuts at: 0 502 1599 Size: 502 1097 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 481 1599 Size: 481 1118 HaeII r_GCGC'y Cuts at: 0 100 116 1599 Size: 100 16 1483 Fragments arranged by size: 1483 100 16 KasI G'GCGC_C Cuts at: 0 96 112 1599 Size: 96 16 1487 Fragments arranged by size: 1487 96 16 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 408 493 1599 Size: 408 85 1106 Fragments arranged by size: 1106 408 85 MscI TGG'CCA Cuts at: 0 1081 1599 Size: 1081 518 MslI CAynn'nnrTG Cuts at: 0 794 1094 1579 1599 Size: 794 300 485 20 Fragments arranged by size: 794 485 300 20 MspA1I CmG'CkG Cuts at: 0 75 267 291 359 1559 1599 Size: 75 192 24 68 1200 40 Fragments arranged by size: 1200 192 75 68 40 24 NaeI GCC'GGC Cuts at: 0 127 231 1599 Size: 127 104 1368 Fragments arranged by size: 1368 127 104 NarI GG'CG_CC Cuts at: 0 97 113 1599 Size: 97 16 1486 Fragments arranged by size: 1486 97 16 NdeI CA'TA_TG Cuts at: 0 422 762 1599 Size: 422 340 837 Fragments arranged by size: 837 422 340 NgoMIV G'CCGG_C Cuts at: 0 125 229 1599 Size: 125 104 1370 Fragments arranged by size: 1370 125 104 PflMI CCAn_nnn'nTGG Cuts at: 0 1339 1599 Size: 1339 260 PfoI T'CCnGG_A Cuts at: 0 242 1599 Size: 242 1357 PpuMI rG'GwC_Cy Cuts at: 0 738 1437 1551 1599 Size: 738 699 114 48 Fragments arranged by size: 738 699 114 48 PspOMI G'GGCC_C Cuts at: 0 1587 1599 Size: 1587 12 PstI C_TGCA'G Cuts at: 0 846 1599 Size: 846 753 PvuII CAG'CTG Cuts at: 0 291 359 1599 Size: 291 68 1240 Fragments arranged by size: 1240 291 68 SacI G_AGCT'C Cuts at: 0 1040 1599 Size: 1040 559 SanDI GG'GwC_CC Cuts at: 0 1437 1599 Size: 1437 162 SexAI A'CCwGG_T Cuts at: 0 137 1599 Size: 137 1462 SfcI C'TryA_G Cuts at: 0 656 842 893 1504 1599 Size: 656 186 51 611 95 Fragments arranged by size: 656 611 186 95 51 SfoI GGC'GCC Cuts at: 0 98 114 1599 Size: 98 16 1485 Fragments arranged by size: 1485 98 16 SspI AAT'ATT Cuts at: 0 1182 1264 1599 Size: 1182 82 335 Fragments arranged by size: 1182 335 82 StyI C'CwwG_G Cuts at: 0 162 1568 1591 1599 Size: 162 1406 23 8 Fragments arranged by size: 1406 162 23 8 TatI w'GTAC_w Cuts at: 0 541 1535 1599 Size: 541 994 64 Fragments arranged by size: 994 541 64 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 295 1224 1599 Size: 295 929 375 Fragments arranged by size: 929 375 295 XmnI GAAnn'nnTTC Cuts at: 0 464 1599 Size: 464 1135 ZraI GAC'GTC Cuts at: 0 177 1599 Size: 177 1422 Enzymes that do cut: AatII AcuI AflIII AleI AlfI AlwNI ApaI ApaLI AseI AvaI AvrII BanI BanII BbeI BbsI BcgI Bme1580I BmgBI BmrI BplI BpmI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrFI BsrGI BstYI Bsu36I BtgI BtsI DraI DraIII EaeI EagI EarI EcoICRI Eco57MI EcoO109I EcoRV FalI HaeII KasI MmeI MscI MslI MspA1I NaeI NarI NdeI NgoMIV PflMI PfoI PpuMI PspOMI PstI PvuII SacI SanDI SexAI SfcI SfoI SspI StyI TatI XcmI XmnI ZraI Enzymes that do not cut: AarI AccI Acc65I AclI AfeI AflII AgeI AhdI AloI ApoI AscI AsiSI BaeI BamHI BbvCI BciVI BclI BfrBI BglI BglII BlpI BmtI Bpu10I BpuEI BsaI BsaAI BsaXI BsiWI BspEI BsrBI BsrDI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I BtgZI ClaI CspCI DrdI EciI EcoNI EcoRI FseI FspI FspAI Hin4I HincII HindIII HpaI KpnI MfeI MluI NcoI NheI NotI NruI NsiI NspI PacI PasI PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI SapI SbfI ScaI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI StuI SwaI TaqII TstI Tth111I XbaI XhoI XmaI