Restriction map for pF1KB6782
##################################
# NO CUT ENZYMES (  196 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AflIII      AgeI        
AhdI        AlwNI       AocI        Aor51HI     ApaI        
AscI        Asp700I     Asp718I     AspEI       AspI        
AvaI        AvaII       AviII       AvrII       BalI        
BamHI       BanI        BanII       BbeI        BbrPI       
BbsI        BbvCI       BbvI        BcgI        BciVI       
BclI        BcnI        BglI        BglII       BlnI        
BlpI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BseRI       
BsgI        BsiEI       BsiWI       BsiYI       BslI        
BsmBI       BsmI        BsoBI       BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrFI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstXI       BstZ17I     
Bsu36I      Cac8I       CelII       CfoI        Cfr10I      
Cfr13I      ClaI        CpoI        DraI        DraII       
DraIII      DrdI        DsaI        EaeI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRI       
EcoRV       EcoT14I     EcoT22I     FauI        FbaI        
Fnu4HI      FseI        FspI        HaeII       HapII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HpaI        HpaII       ItaI        
KasI        KpnI        Ksp632I     KspI        MamI        
MfeI        MluI        MluNI       MroI        MscI        
MspA1I      MspI        MunI        MvnI        MwoI        
NaeI        NarI        NciI        NcoI        NdeI        
NgoMIV      NheI        NlaIV       NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PleI        PmaCI       PmeI        
PmlI        PpuMI       PshAI       Psp1406I    PstI        
PvuI        PvuII       RcaI        RsrII       SacI        
SacII       SalI        SapI        Sau96I      SbfI        
ScaI        SexAI       SfcI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspI        StuI        StyI        
SwaI        TaqI        TseI        Tth111I     TthHB8I     
Van91I      XcmI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AfaI            3  GTAC                 GT^AC
AflII         297  CTTAAG               C^TTAAG
Alw44I         59  GTGCAC               G^TGCAC
AlwI          352  GGATC                GGATC(4/5)
ApaLI          59  GTGCAC               G^TGCAC
AseI          142  ATTAAT               AT^TAAT
AsnI          142  ATTAAT               AT^TAAT
AspHI          59  GTGCAC               G[AT]GC[AT]^C
BfaI          235  CTAG                 C^TAG
BfrI          297  CTTAAG               C^TTAAG
BmyI           59  GTGCAC               G[AGT]GC[ACT]^C
BsiHKAI        59  GTGCAC               G[AT]GC[AT]^C
BsmAI         388  GAGAC                GTCTC(1/5)
BsmFI         308  GGGAC                GGGAC(10/14)
Bsp1286I       59  GTGCAC               G[AGT]GC[ACT]^C
Bsp1407I        2  TGTACA               T^GTACA
BsrDI          27  CATTGC               GCAATG(2/0)
BsrGI           2  TGTACA               T^GTACA
BsrI           99  ACTGG                ACTGG(1/-1)
BstYI         352  GGATCT               [GA]^GATC[CT]
DdeI           34  CTAAG                C^T[ACGT]AG
DpnII         353  GATC                 ^GATC
HaeIII        207  GGCC                 GG^CC
HinfI         156  GAATC                G^A[ACGT]TC
HphI          167  TCACC                GGTGA(8/7)
MaeI          235  CTAG                 C^TAG
MaeII         329  ACGT                 A^CGT
MaeIII         49  GTCAC                ^GT[ACGT]AC
MboI          353  GATC                 ^GATC
MflI          352  GGATCT               [GA]^GATC[CT]
NdeII         353  GATC                 ^GATC
NspI          194  ACATGC               [GA]CATG^[CT]
PshBI         142  ATTAAT               AT^TAAT
RsaI            3  GTAC                 GT^AC
Sau3AI        353  GATC                 ^GATC
SmlI          297  CTTAAG               C^T[CT][GA]AG
SspBI           2  TGTACA               T^GTACA
TfiI          156  GAATC                G^A[AT]TC
Tsp45I         49  GTCAC                ^GT[GC]AC
XbaI          234  TCTAGA               T^CTAGA
XhoII         352  GGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

AcsI          270  AAATTT               [GA]^AATT[CT]
              406  GAATTT              
AluI           92  AGCT                 AG^CT
              276  AGCT                
ApoI          270  AAATTT               [GA]^AATT[CT]
              406  GAATTT              
BpmI          100  CTGGAG               CTGGAG(16/14)
              256  CTGGAG              
BsaJI         304  CCCAGG               C^C[ACGT][ACGT]GG
              305  CCAGGG              
BssKI         170  CCAGG                ^CC[ACGT]GG
              305  CCAGG               
BstF5I          8  GGATG                GGATG(2/0)
              173  GGATG               
BstNI         170  CCAGG                CC^[AT]GG
              305  CCAGG               
EcoRII        170  CCAGG                ^CC[AT]GG
              305  CCAGG               
FokI            8  GGATG                GGATG(9/13)
              173  GGATG               
MnlI          253  CCTC                 CCTC(7/6)
              259  GAGG                
MslI          168  CACCAGGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              365  CATTCATGTG          
MvaI          170  CCAGG                CC^[AT]GG
              305  CCAGG               
NlaIII        195  CATG                 CATG^
              369  CATG                
ScrFI         170  CCAGG                CC^[ACGT]GG
              305  CCAGG               


#########################################
# 3 OR MORE CUT ENZYMES (    6 enzymes) #
#########################################

MboII       MseI        SfaNI       Tru9I       Tsp509I     
TspRI       
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