(Circular) (Six-base) MAPSORT of: pF1KE2112.seq Check: 7619 from: 1 to: 5876 pF1KE2112 5876 bp With 182 enzymes: SgfI * February 10, 2017 14:54 .. AccI GT'mk_AC Cuts at: 2702 2914 2702 Size: 212 5664 Acc65I G'GTAC_C Cuts at: 2892 2892 Size: 5876 AclI AA'CG_TT Cuts at: 5716 5716 Size: 5876 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 933 1590 1786 1961 2234 2512 3701 4133 4880 Size: 657 196 175 273 278 1189 432 747 Cuts at: 4880 5148 933 Size: 268 1661 Fragments arranged by size: 1661 1189 747 657 432 278 273 268 196 175 AfeI AGC'GCT Cuts at: 451 451 Size: 5876 AflIII A'CryG_T Cuts at: 2644 3228 4332 5309 2644 Size: 584 1104 977 3211 Fragments arranged by size: 3211 1104 977 584 AgeI A'CCGG_T Cuts at: 4264 4264 Size: 5876 AlwNI CAG_nnn'CTG Cuts at: 750 1585 3181 4748 750 Size: 835 1596 1567 1878 Fragments arranged by size: 1878 1596 1567 835 ApaI G_GGCC'C Cuts at: 400 400 Size: 5876 ApaLI G'TGCA_C Cuts at: 853 4646 853 Size: 3793 2083 ApoI r'AATT_y Cuts at: 1457 2248 2880 5229 5485 1457 Size: 791 632 2349 256 1848 Fragments arranged by size: 2349 1848 791 632 256 AseI AT'TA_AT Cuts at: 20 2336 20 Size: 2316 3560 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 5876 AvaI C'yCGr_G Cuts at: 963 2896 963 Size: 1933 3943 AvrII C'CTAG_G Cuts at: 1708 1708 Size: 5876 BamHI G'GATC_C Cuts at: 2901 2901 Size: 5876 BanI G'GyrC_C Cuts at: 159 261 380 424 874 2892 3306 3554 3589 Size: 102 119 44 450 2018 414 248 35 Cuts at: 3589 159 Size: 2446 Fragments arranged by size: 2446 2018 450 414 248 119 102 44 35 BanII G_rGCy'C Cuts at: 400 736 1049 1694 2890 3920 400 Size: 336 313 645 1196 1030 2356 Fragments arranged by size: 2356 1196 1030 645 336 313 BbeI G_GCGC'C Cuts at: 163 265 384 3310 3558 163 Size: 102 119 2926 248 2481 Fragments arranged by size: 2926 2481 248 119 102 BbsI GAAGACnn'nnnn_ Cuts at: 182 2792 182 Size: 2610 3266 BciVI GTATCCnnnnn_n' Cuts at: 2260 3774 4535 5876 2260 Size: 1514 761 1341 2260 Fragments arranged by size: 2260 1514 1341 761 BclI T'GATC_A Cuts at: 2241 3396 2241 Size: 1155 4721 BglI GCCn_nnn'nGGC Cuts at: 221 997 1069 221 Size: 776 72 5028 Fragments arranged by size: 5028 776 72 BglII A'GATC_T Cuts at: 1194 1601 2027 3391 5428 1194 Size: 407 426 1364 2037 1642 Fragments arranged by size: 2037 1642 1364 426 407 BlpI GC'TnA_GC Cuts at: 1048 1969 2988 1048 Size: 921 1019 3936 Fragments arranged by size: 3936 1019 921 Bme1580I G_kGCm'C Cuts at: 400 857 3501 3594 4650 400 Size: 457 2644 93 1056 1626 Fragments arranged by size: 2644 1626 1056 457 93 BmrI ACTGGGnnnn_n' Cuts at: 478 1617 3088 3169 3504 5648 478 Size: 1139 1471 81 335 2144 706 Fragments arranged by size: 2144 1471 1139 706 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 273 1356 4243 273 Size: 1083 2887 1906 Fragments arranged by size: 2887 1906 1083 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 246 268 523 607 1237 2745 246 Size: 22 255 84 630 1508 3377 Fragments arranged by size: 3377 1508 630 255 84 22 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 592 2580 3052 3931 4423 4721 592 Size: 1988 472 879 492 298 1747 Fragments arranged by size: 1988 1747 879 492 472 298 BsaI GGTCTCn'nnnn_ Cuts at: 5580 5580 Size: 5876 BsaAI yAC'GTr Cuts at: 669 3229 669 Size: 2560 3316 BsaBI GATnn'nnATC Cuts at: 6 3390 5427 6 Size: 3384 2037 455 Fragments arranged by size: 3384 2037 455 BsaHI Gr'CG_yC Cuts at: 160 233 262 381 461 638 3307 3555 4257 Size: 73 29 119 80 177 2669 248 702 Cuts at: 4257 5753 160 Size: 1496 283 Fragments arranged by size: 2669 1496 702 283 248 177 119 80 73 29 BsaWI w'CCGG_w Cuts at: 3065 3586 4264 4538 4685 5096 3065 Size: 521 678 274 147 411 3845 Fragments arranged by size: 3845 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 2421 2451 2421 Size: 30 5846 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 181 184 457 460 520 181 Size: 3 273 3 60 5537 Fragments arranged by size: 5537 273 60 3 3 BseYI C'CCAG_C Cuts at: 1449 2851 3301 4059 4636 5132 1449 Size: 1402 450 758 577 496 2193 Fragments arranged by size: 2193 1402 758 577 496 450 BsiEI CG_ry'CG Cuts at: 104 892 3418 3464 4672 104 Size: 788 2526 46 1208 1308 Fragments arranged by size: 2526 1308 1208 788 46 BsiHKAI G_wGCw'C Cuts at: 584 857 1049 1694 1970 2234 2890 3668 3858 Size: 273 192 645 276 264 656 778 190 Cuts at: 3858 4650 584 Size: 792 1810 Fragments arranged by size: 1810 792 778 656 645 276 273 264 192 190 BsmI GAATG_Cn' Cuts at: 2627 2627 Size: 5876 BsmBI CGTCTCn'nnnn_ Cuts at: 342 727 342 Size: 385 5491 Bsp1286I G_dGCh'C Cuts at: 400 584 736 857 1049 1694 1970 2234 2890 Size: 184 152 121 192 645 276 264 656 Cuts at: 2890 3501 3594 3668 3858 3920 4650 400 Size: 611 93 74 190 62 730 1626 Fragments arranged by size: 1626 730 656 645 611 276 264 192 190 184 152 121 93 74 62 BspEI T'CCGG_A Cuts at: 5096 5096 Size: 5876 BspHI T'CATG_A Cuts at: 5052 5052 Size: 5876 BspMI ACCTGCnnnn'nnnn_ Cuts at: 2926 3140 3442 3823 2926 Size: 214 302 381 4979 Fragments arranged by size: 4979 381 302 214 BsrBI CCG'CTC Cuts at: 50 888 4168 4222 5706 5871 50 Size: 838 3280 54 1484 165 55 Fragments arranged by size: 3280 1484 838 165 55 54 BsrDI GCAATG_nn' Cuts at: 1126 1766 3788 1126 Size: 640 2022 3214 Fragments arranged by size: 3214 2022 640 BsrFI r'CCGG_y Cuts at: 377 3874 4055 4264 377 Size: 3497 181 209 1989 Fragments arranged by size: 3497 1989 209 181 BssHII G'CGCG_C Cuts at: 625 625 Size: 5876 BssSI C'ACGA_G Cuts at: 4147 4505 4147 Size: 358 5518 BstAPI GCAn_nnn'nTGC Cuts at: 750 861 3253 750 Size: 111 2392 3373 Fragments arranged by size: 3373 2392 111 BstBI TT'CG_AA Cuts at: 1550 4237 1550 Size: 2687 3189 BstEII G'GTnAC_C Cuts at: 147 591 147 Size: 444 5432 BstXI CCAn_nnnn'nTGG Cuts at: 822 822 Size: 5876 BstYI r'GATC_y Cuts at: 1 1149 1194 1601 2027 2901 3368 3391 3726 Size: 1148 45 407 426 874 467 23 335 Cuts at: 3726 3972 4984 5070 5082 5428 1 Size: 246 1012 86 12 346 449 Fragments arranged by size: 1148 1012 874 467 449 426 407 346 335 246 86 45 23 12 Bsu36I CC'TnA_GG Cuts at: 1348 1348 Size: 5876 BtgI C'CryG_G Cuts at: 309 326 420 309 Size: 17 94 5765 Fragments arranged by size: 5765 94 17 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 3826 4007 4170 3826 Size: 181 163 5532 Fragments arranged by size: 5532 181 163 BtsI GCAGTG_nn' Cuts at: 831 3119 831 Size: 2288 3588 DraI TTT'AAA Cuts at: 2875 2875 Size: 5876 DrdI GACnn_nn'nnGTC Cuts at: 1837 3582 4440 1837 Size: 1745 858 3273 Fragments arranged by size: 3273 1745 858 EaeI y'GGCC_r Cuts at: 710 889 989 1061 1070 1415 3461 3635 4026 Size: 179 100 72 9 345 2046 174 391 Cuts at: 4026 4053 5398 710 Size: 27 1345 1188 Fragments arranged by size: 2046 1345 1188 391 345 179 174 100 72 27 9 EagI C'GGCC_G Cuts at: 889 3461 889 Size: 2572 3304 EarI CTCTTCn'nnn_ Cuts at: 1163 2584 3899 4109 1163 Size: 1421 1315 210 2930 Fragments arranged by size: 2930 1421 1315 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 4084 4394 4540 5686 4084 Size: 310 146 1146 4274 Fragments arranged by size: 4274 1146 310 146 EcoICRI GAG'CTC Cuts at: 1047 1692 2888 1047 Size: 645 1196 4035 Fragments arranged by size: 4035 1196 645 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 246 268 523 607 933 1237 1590 1786 1961 Size: 22 255 84 326 304 353 196 175 Cuts at: 1961 2234 2512 2745 3701 4133 4880 5148 246 Size: 273 278 233 956 432 747 268 974 Fragments arranged by size: 974 956 747 432 353 326 304 278 273 268 255 233 196 175 84 22 EcoNI CCTnn'n_nnAGG Cuts at: 1542 1542 Size: 5876 EcoO109I rG'GnC_Cy Cuts at: 396 1023 1705 3015 396 Size: 627 682 1310 3257 Fragments arranged by size: 3257 1310 682 627 EcoRI G'AATT_C Cuts at: 2880 2880 Size: 5876 EcoRV GAT'ATC Cuts at: 2757 2757 Size: 5876 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3895 3895 Size: 5876 FspI TGC'GCA Cuts at: 493 673 3657 493 Size: 180 2984 2712 Fragments arranged by size: 2984 2712 180 FspAI rTGC'GCAy Cuts at: 673 673 Size: 5876 HaeII r_GCGC'y Cuts at: 163 213 265 384 453 3310 3558 4580 5559 Size: 50 52 119 69 2857 248 1022 979 Cuts at: 5559 163 Size: 480 Fragments arranged by size: 2857 1022 979 480 248 119 69 52 50 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1186 1218 3718 3748 3750 3780 1186 Size: 32 2500 30 2 30 3282 Fragments arranged by size: 3282 2500 32 30 30 2 HincII GTy'rAC Cuts at: 237 528 2499 2915 237 Size: 291 1971 416 3198 Fragments arranged by size: 3198 1971 416 291 HindIII A'AGCT_T Cuts at: 1235 1235 Size: 5876 HpaI GTT'AAC Cuts at: 2499 2499 Size: 5876 KasI G'GCGC_C Cuts at: 159 261 380 3306 3554 159 Size: 102 119 2926 248 2481 Fragments arranged by size: 2926 2481 248 119 102 KpnI G_GTAC'C Cuts at: 2896 2896 Size: 5876 MluI A'CGCG_T Cuts at: 5309 5309 Size: 5876 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 4547 4731 4547 Size: 184 5692 MscI TGG'CCA Cuts at: 991 1063 1072 1417 3637 991 Size: 72 9 345 2220 3230 Fragments arranged by size: 3230 2220 345 72 9 MslI CAynn'nnrTG Cuts at: 358 484 3865 3992 358 Size: 126 3381 127 2242 Fragments arranged by size: 3381 2242 127 126 MspA1I CmG'CkG Cuts at: 273 422 564 2987 3301 3661 4674 4919 273 Size: 149 142 2423 314 360 1013 245 1230 Fragments arranged by size: 2423 1230 1013 360 314 245 149 142 NaeI GCC'GGC Cuts at: 379 4057 379 Size: 3678 2198 NarI GG'CG_CC Cuts at: 160 262 381 3307 3555 160 Size: 102 119 2926 248 2481 Fragments arranged by size: 2926 2481 248 119 102 NcoI C'CATG_G Cuts at: 326 326 Size: 5876 NgoMIV G'CCGG_C Cuts at: 377 4055 377 Size: 3678 2198 NotI GC'GGCC_GC Cuts at: 889 889 Size: 5876 NspI r_CATG'y Cuts at: 861 1495 2112 2322 2859 2929 3429 4336 861 Size: 634 617 210 537 70 500 907 2401 Fragments arranged by size: 2401 907 634 617 537 500 210 70 PasI CC'CwG_GG Cuts at: 1398 1398 Size: 5876 PciI A'CATG_T Cuts at: 4332 4332 Size: 5876 PmeI GTTT'AAAC Cuts at: 2875 2875 Size: 5876 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 1072 1104 5015 5047 1072 Size: 32 3911 32 1901 Fragments arranged by size: 3911 1901 32 32 PpuMI rG'GwC_Cy Cuts at: 1023 1705 1023 Size: 682 5194 PsiI TTA'TAA Cuts at: 2674 2674 Size: 5876 PspOMI G'GGCC_C Cuts at: 396 396 Size: 5876 PspXI vC'TCGA_Gb Cuts at: 963 963 Size: 5876 PstI C_TGCA'G Cuts at: 563 755 2027 2923 3608 563 Size: 192 1272 896 685 2831 Fragments arranged by size: 2831 1272 896 685 192 PvuI CG_AT'CG Cuts at: 104 104 Size: 5876 PvuII CAG'CTG Cuts at: 564 3301 3661 564 Size: 2737 360 2779 Fragments arranged by size: 2779 2737 360 RsrII CG'GwC_CG Cuts at: 4071 4071 Size: 5876 SacI G_AGCT'C Cuts at: 1049 1694 2890 1049 Size: 645 1196 4035 Fragments arranged by size: 4035 1196 645 SacII CC_GC'GG Cuts at: 423 423 Size: 5876 SalI G'TCGA_C Cuts at: 2913 2913 Size: 5876 SapI GCTCTTCn'nnn_ Cuts at: 3899 4109 3899 Size: 210 5666 SbfI CC_TGCA'GG Cuts at: 2923 2923 Size: 5876 ScaI AGT'ACT Cuts at: 5393 5393 Size: 5876 SfcI C'TryA_G Cuts at: 33 559 751 2023 2165 2919 3604 4597 4788 Size: 526 192 1272 142 754 685 993 191 Cuts at: 4788 33 Size: 1121 Fragments arranged by size: 1272 1121 993 754 685 526 192 191 142 SfiI GGCCn_nnn'nGGCC Cuts at: 1069 1069 Size: 5876 SfoI GGC'GCC Cuts at: 161 263 382 3308 3556 161 Size: 102 119 2926 248 2481 Fragments arranged by size: 2926 2481 248 119 102 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 5876 SmaI CCC'GGG Cuts at: 2898 2898 Size: 5876 SmlI C'TyrA_G Cuts at: 607 963 2595 3031 3946 4438 4700 607 Size: 356 1632 436 915 492 262 1783 Fragments arranged by size: 1783 1632 915 492 436 356 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 5876 SphI G_CATG'C Cuts at: 2859 2929 3429 2859 Size: 70 500 5306 Fragments arranged by size: 5306 500 70 SspI AAT'ATT Cuts at: 2201 2262 2201 Size: 61 5815 StuI AGG'CCT Cuts at: 1346 1946 1346 Size: 600 5276 StyI C'CwwG_G Cuts at: 116 326 1708 2133 3010 3364 116 Size: 210 1382 425 877 354 2628 Fragments arranged by size: 2628 1382 877 425 354 210 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 610 1129 1636 3459 3592 4051 4260 610 Size: 519 507 1823 133 459 209 2226 Fragments arranged by size: 2226 1823 519 507 459 209 133 TatI w'GTAC_w Cuts at: 5391 5391 Size: 5876 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5393 5425 5393 Size: 32 5844 Tth111I GACn'n_nGTC Cuts at: 3673 3673 Size: 5876 XbaI T'CTAG_A Cuts at: 2907 2907 Size: 5876 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3371 3371 Size: 5876 XhoI C'TCGA_G Cuts at: 963 963 Size: 5876 XmaI C'CCGG_G Cuts at: 2896 2896 Size: 5876 XmnI GAAnn'nnTTC Cuts at: 1087 1181 1087 Size: 94 5782 Enzymes that do cut: AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NotI NspI PasI PciI PmeI PpiI PpuMI PsiI PspOMI PspXI PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI Enzymes that do not cut: AarI AatII AflII AhdI AleI AlfI AloI AscI BaeI BbvCI BcgI BfrBI BmgBI BmtI Bpu10I BsgI BsiWI BsrGI BstZ17I ClaI CspCI DraIII FseI MfeI NdeI NheI NruI NsiI PacI PflMI PfoI PmlI PshAI PsrI SanDI SexAI SgrAI SnaBI SrfI SwaI ZraI