(Linear) (Six-base) MAPSORT of: KIEE3542.seq Check: 5790 from: 1 to: 3390 KIEE3542.orf With 182 enzymes: SgfI * March 22, 2013 15:27 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 84 232 1686 2188 3390 Size: 84 148 1454 502 1202 Fragments arranged by size: 1454 1202 502 148 84 AccI GT'mk_AC Cuts at: 0 715 3390 Size: 715 2675 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1022 1262 3390 Size: 1022 240 2128 Fragments arranged by size: 2128 1022 240 AflII C'TTAA_G Cuts at: 0 1795 3390 Size: 1795 1595 AhdI GACnn_n'nnGTC Cuts at: 0 1497 3325 3390 Size: 1497 1828 65 Fragments arranged by size: 1828 1497 65 AleI CACnn'nnGTG Cuts at: 0 3109 3390 Size: 3109 281 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1694 3390 Size: 1694 1696 AlwNI CAG_nnn'CTG Cuts at: 0 2767 3390 Size: 2767 623 ApoI r'AATT_y Cuts at: 0 304 578 595 697 797 1867 1873 2143 Size: 304 274 17 102 100 1070 6 270 Cuts at: 2143 2171 2203 2836 3046 3390 Size: 28 32 633 210 344 Fragments arranged by size: 1070 633 344 304 274 270 210 102 100 32 28 17 6 AvaI C'yCGr_G Cuts at: 0 112 136 2082 3390 Size: 112 24 1946 1308 Fragments arranged by size: 1946 1308 112 24 AvrII C'CTAG_G Cuts at: 0 271 1285 3390 Size: 271 1014 2105 Fragments arranged by size: 2105 1014 271 BamHI G'GATC_C Cuts at: 0 2551 3390 Size: 2551 839 BanI G'GyrC_C Cuts at: 0 39 131 3390 Size: 39 92 3259 Fragments arranged by size: 3259 92 39 BanII G_rGCy'C Cuts at: 0 34 1339 3390 Size: 34 1305 2051 Fragments arranged by size: 2051 1305 34 BbeI G_GCGC'C Cuts at: 0 43 135 3390 Size: 43 92 3255 Fragments arranged by size: 3255 92 43 BbsI GAAGACnn'nnnn_ Cuts at: 0 194 2126 2239 3390 Size: 194 1932 113 1151 Fragments arranged by size: 1932 1151 194 113 BbvCI CC'TCA_GC Cuts at: 0 2844 3390 Size: 2844 546 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 52 86 715 749 3390 Size: 52 34 629 34 2641 Fragments arranged by size: 2641 629 52 34 34 BciVI GTATCCnnnnn_n' Cuts at: 0 1840 3390 Size: 1840 1550 BclI T'GATC_A Cuts at: 0 1466 1991 3390 Size: 1466 525 1399 Fragments arranged by size: 1466 1399 525 BfrBI ATG'CAT Cuts at: 0 2857 3390 Size: 2857 533 BglII A'GATC_T Cuts at: 0 1365 3390 Size: 1365 2025 Bme1580I G_kGCm'C Cuts at: 0 106 3099 3390 Size: 106 2993 291 Fragments arranged by size: 2993 291 106 BmgBI CAC'GTC Cuts at: 0 192 369 3390 Size: 192 177 3021 Fragments arranged by size: 3021 192 177 BmrI ACTGGGnnnn_n' Cuts at: 0 92 421 1647 1769 2006 2251 3086 3390 Size: 92 329 1226 122 237 245 835 304 Fragments arranged by size: 1226 835 329 304 245 237 122 92 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1577 3390 Size: 1577 1813 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2 2724 3390 Size: 2 2722 666 Fragments arranged by size: 2722 666 2 Bpu10I CC'TnA_GC Cuts at: 0 2844 3390 Size: 2844 546 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2151 3390 Size: 2151 1239 BsaI GGTCTCn'nnnn_ Cuts at: 0 2666 2740 2898 3390 Size: 2666 74 158 492 Fragments arranged by size: 2666 492 158 74 BsaAI yAC'GTr Cuts at: 0 1506 3390 Size: 1506 1884 BsaHI Gr'CG_yC Cuts at: 0 40 132 3390 Size: 40 92 3258 Fragments arranged by size: 3258 92 40 BsaWI w'CCGG_w Cuts at: 0 3153 3390 Size: 3153 237 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 541 571 2875 2905 3390 Size: 541 30 2304 30 485 Fragments arranged by size: 2304 541 485 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 42 48 178 187 190 193 951 1954 Size: 42 6 130 9 3 3 758 1003 Cuts at: 1954 2832 3390 Size: 878 558 Fragments arranged by size: 1003 878 758 558 130 42 9 6 3 3 BseYI C'CCAG_C Cuts at: 0 1144 2422 3390 Size: 1144 1278 968 Fragments arranged by size: 1278 1144 968 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 56 3390 Size: 56 3334 BsiHKAI G_wGCw'C Cuts at: 0 34 3390 Size: 34 3356 BsmI GAATG_Cn' Cuts at: 0 2618 3390 Size: 2618 772 BsmBI CGTCTCn'nnnn_ Cuts at: 0 375 3312 3390 Size: 375 2937 78 Fragments arranged by size: 2937 375 78 Bsp1286I G_dGCh'C Cuts at: 0 34 106 1339 3099 3390 Size: 34 72 1233 1760 291 Fragments arranged by size: 1760 1233 291 72 34 BspEI T'CCGG_A Cuts at: 0 3153 3390 Size: 3153 237 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 84 232 1686 2188 3390 Size: 84 148 1454 502 1202 Fragments arranged by size: 1454 1202 502 148 84 BsrDI GCAATG_nn' Cuts at: 0 863 1538 1655 2624 3390 Size: 863 675 117 969 766 Fragments arranged by size: 969 863 766 675 117 BssSI C'ACGA_G Cuts at: 0 99 1927 3390 Size: 99 1828 1463 Fragments arranged by size: 1828 1463 99 BstAPI GCAn_nnn'nTGC Cuts at: 0 2472 2854 3390 Size: 2472 382 536 Fragments arranged by size: 2472 536 382 BstXI CCAn_nnnn'nTGG Cuts at: 0 2406 2748 3390 Size: 2406 342 642 Fragments arranged by size: 2406 642 342 BstYI r'GATC_y Cuts at: 0 554 1365 2252 2386 2485 2551 3390 Size: 554 811 887 134 99 66 839 Fragments arranged by size: 887 839 811 554 134 99 66 BstZ17I GTA'TAC Cuts at: 0 716 3390 Size: 716 2674 Bsu36I CC'TnA_GG Cuts at: 0 2431 3390 Size: 2431 959 BtgI C'CryG_G Cuts at: 0 281 625 687 1592 2975 3390 Size: 281 344 62 905 1383 415 Fragments arranged by size: 1383 905 415 344 281 62 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 2808 2867 3390 Size: 2808 59 523 Fragments arranged by size: 2808 523 59 BtsI GCAGTG_nn' Cuts at: 0 3012 3390 Size: 3012 378 DraI TTT'AAA Cuts at: 0 2447 3390 Size: 2447 943 DraIII CAC_nnn'GTG Cuts at: 0 1345 3390 Size: 1345 2045 EaeI y'GGCC_r Cuts at: 0 349 923 2283 2396 2583 3390 Size: 349 574 1360 113 187 807 Fragments arranged by size: 1360 807 574 349 187 113 EarI CTCTTCn'nnn_ Cuts at: 0 164 988 998 1758 2033 2038 2298 3390 Size: 164 824 10 760 275 5 260 1092 Fragments arranged by size: 1092 824 760 275 260 164 10 5 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1967 3390 Size: 1967 1423 EcoICRI GAG'CTC Cuts at: 0 32 3390 Size: 32 3358 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2 1022 1262 2724 3390 Size: 2 1020 240 1462 666 Fragments arranged by size: 1462 1020 666 240 2 EcoNI CCTnn'n_nnAGG Cuts at: 0 123 3112 3390 Size: 123 2989 278 Fragments arranged by size: 2989 278 123 EcoO109I rG'GnC_Cy Cuts at: 0 116 201 811 2460 3390 Size: 116 85 610 1649 930 Fragments arranged by size: 1649 930 610 116 85 EcoRI G'AATT_C Cuts at: 0 2171 3390 Size: 2171 1219 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 3240 3390 Size: 3240 150 HaeII r_GCGC'y Cuts at: 0 43 135 3390 Size: 43 92 3255 Fragments arranged by size: 3255 92 43 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 946 978 1350 1382 1483 1515 1581 1613 Size: 946 32 372 32 101 32 66 32 Cuts at: 1613 1627 1659 1910 1942 1946 1978 2536 2568 Size: 14 32 251 32 4 32 558 32 Cuts at: 2568 2875 2907 3072 3104 3311 3343 3390 Size: 307 32 165 32 207 32 47 Fragments arranged by size: 946 558 372 307 251 207 165 101 66 47 32 32 32 32 32 32 32 32 32 32 32 14 4 HindIII A'AGCT_T Cuts at: 0 1136 3390 Size: 1136 2254 KasI G'GCGC_C Cuts at: 0 39 131 3390 Size: 39 92 3259 Fragments arranged by size: 3259 92 39 MfeI C'AATT_G Cuts at: 0 731 3390 Size: 731 2659 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 398 2589 3390 Size: 398 2191 801 Fragments arranged by size: 2191 801 398 MscI TGG'CCA Cuts at: 0 351 925 2285 2585 3390 Size: 351 574 1360 300 805 Fragments arranged by size: 1360 805 574 351 300 MslI CAynn'nnrTG Cuts at: 0 466 1828 2404 2875 3109 3390 Size: 466 1362 576 471 234 281 Fragments arranged by size: 1362 576 471 466 281 234 MspA1I CmG'CkG Cuts at: 0 70 1743 1945 3003 3390 Size: 70 1673 202 1058 387 Fragments arranged by size: 1673 1058 387 202 70 NarI GG'CG_CC Cuts at: 0 40 132 3390 Size: 40 92 3258 Fragments arranged by size: 3258 92 40 NcoI C'CATG_G Cuts at: 0 281 625 2975 3390 Size: 281 344 2350 415 Fragments arranged by size: 2350 415 344 281 NdeI CA'TA_TG Cuts at: 0 2115 3390 Size: 2115 1275 NsiI A_TGCA'T Cuts at: 0 2859 3390 Size: 2859 531 NspI r_CATG'y Cuts at: 0 1265 3390 Size: 1265 2125 PflMI CCAn_nnn'nTGG Cuts at: 0 445 2804 3390 Size: 445 2359 586 Fragments arranged by size: 2359 586 445 PfoI T'CCnGG_A Cuts at: 0 299 3390 Size: 299 3091 PmlI CAC'GTG Cuts at: 0 1506 3390 Size: 1506 1884 PpuMI rG'GwC_Cy Cuts at: 0 116 201 3390 Size: 116 85 3189 Fragments arranged by size: 3189 116 85 PshAI GACnn'nnGTC Cuts at: 0 362 3390 Size: 362 3028 PstI C_TGCA'G Cuts at: 0 364 1078 3390 Size: 364 714 2312 Fragments arranged by size: 2312 714 364 PvuII CAG'CTG Cuts at: 0 70 1743 3003 3390 Size: 70 1673 1260 387 Fragments arranged by size: 1673 1260 387 70 SacI G_AGCT'C Cuts at: 0 34 3390 Size: 34 3356 SapI GCTCTTCn'nnn_ Cuts at: 0 998 3390 Size: 998 2392 ScaI AGT'ACT Cuts at: 0 1487 1720 3390 Size: 1487 233 1670 Fragments arranged by size: 1670 1487 233 SexAI A'CCwGG_T Cuts at: 0 500 3390 Size: 500 2890 SfcI C'TryA_G Cuts at: 0 360 815 1074 1857 2217 2829 3390 Size: 360 455 259 783 360 612 561 Fragments arranged by size: 783 612 561 455 360 360 259 SfoI GGC'GCC Cuts at: 0 41 133 3390 Size: 41 92 3257 Fragments arranged by size: 3257 92 41 SmaI CCC'GGG Cuts at: 0 114 138 2084 3390 Size: 114 24 1946 1306 Fragments arranged by size: 1946 1306 114 24 SmlI C'TyrA_G Cuts at: 0 1795 2166 3390 Size: 1795 371 1224 Fragments arranged by size: 1795 1224 371 StuI AGG'CCT Cuts at: 0 1463 3390 Size: 1463 1927 StyI C'CwwG_G Cuts at: 0 271 281 625 946 1285 2975 3390 Size: 271 10 344 321 339 1690 415 Fragments arranged by size: 1690 415 344 339 321 271 10 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 437 757 3390 Size: 437 320 2633 Fragments arranged by size: 2633 437 320 TatI w'GTAC_w Cuts at: 0 408 1078 1485 1718 3390 Size: 408 670 407 233 1672 Fragments arranged by size: 1672 670 408 407 233 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 546 578 3390 Size: 546 32 2812 Fragments arranged by size: 2812 546 32 Tth111I GACn'n_nGTC Cuts at: 0 3058 3390 Size: 3058 332 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 446 465 2062 3390 Size: 446 19 1597 1328 Fragments arranged by size: 1597 1328 446 19 XmaI C'CCGG_G Cuts at: 0 112 136 2082 3390 Size: 112 24 1946 1308 Fragments arranged by size: 1946 1308 112 24 XmnI GAAnn'nnTTC Cuts at: 0 3223 3390 Size: 3223 167 Enzymes that do cut: AarI AccI AcuI AflII AhdI AleI AlfI AlwNI ApoI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglII Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI BsrDI BssSI BstAPI BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI DraI DraIII EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I HindIII KasI MfeI MmeI MscI MslI MspA1I NarI NcoI NdeI NsiI NspI PflMI PfoI PmlI PpuMI PshAI PstI PvuII SacI SapI ScaI SexAI SfcI SfoI SmaI SmlI StuI StyI TaqII TatI TstI Tth111I XcmI XmaI XmnI Enzymes that do not cut: AatII Acc65I AclI AfeI AflIII AgeI AloI ApaI ApaLI AscI AseI AsiSI BaeI BglI BlpI BmtI BsaBI BsiEI BsiWI BspHI BsrBI BsrFI BsrGI BssHII BstBI BstEII ClaI CspCI DrdI EagI EcoRV FseI FspI FspAI HincII HpaI KpnI MluI NaeI NgoMIV NheI NotI NruI PacI PasI PciI PmeI PpiI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI XbaI XhoI ZraI