Restriction map for pF1KE0800
##################################
# NO CUT ENZYMES (  100 enzymes) #
##################################

AatII       AccI        AclI        AcyI        AflII       
Alw44I      Aor51HI     ApaI        ApaLI       AscI        
AspHI       AspI        AviII       BalI        BanII       
BbeI        BbrPI       BbvCI       BcgI        BcnI        
BfrI        BglI        BsaHI       BsaI        BsgI        
BsiEI       BsiHKAI     BsiWI       BsmBI       BspDI       
BspHI       BspMI       BsrBI       BssHII      Bst1107I    
BstAPI      BstEII      BstPI       BstZ17I     CfoI        
ClaI        CpoI        DrdI        EaeI        EagI        
EclXI       Eco47III    Eco52I      EcoO65I     EcoRV       
EcoT22I     FauI        FseI        FspI        HaeII       
HhaI        Hin1I       HinP1I      KasI        KspI        
MluI        MluNI       MscI        NaeI        NarI        
NciI        NgoMIV      NheI        NotI        NruI        
NsiI        PaeR7I      PflFI       PflMI       PmaCI       
PmeI        PmlI        PshAI       Psp1406I    PvuI        
RcaI        RsrII       SacI        SacII       SalI        
SbfI        ScaI        SfiI        SfoI        SgrAI       
SmaI        SnaBI       SphI        Sse8387I    SwaI        
Tth111I     Van91I      XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   55 enzymes) #
###################################

Acc65I       2371  GGTACC               G^GTACC
AccII        3641  CGCG                 CG^CG
AccIII        330  TCCGGA               T^CCGGA
AgeI         4288  ACCGGT               A^CCGGT
AhdI          474  GACAGGTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI         2041  CCTGAGG              CC^T[ACGT]AGG
Asp718I      2371  GGTACC               G^GTACC
AspEI         474  GACAGGTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI         4523  CTCGGG               C^[CT]CG[GA]G
AvrII        3111  CCTAGG               C^CTAGG
BciVI        1904  GTATCC               GTATCC(6/5)
BglII        2137  AGATCT               A^GATCT
BlnI         3111  CCTAGG               C^CTAGG
BlpI         2427  GCTCAGC              GC^T[ACGT]AGC
BmyI         1848  GGGCAC               G[AGT]GC[ACT]^C
BpmI         1708  CTGGAG               CTGGAG(16/14)
Bpu1102I     2427  GCTCAGC              GC^T[ACGT]AGC
BsaAI        3013  CACGTA               [CT]AC^GT[GA]
BsaBI        3247  GATTCGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI         330  TCCGGA               T^CCGGA
BsmI         3116  GAATGC               GAATGC(1/-1)
BsoBI        4523  CTCGGG               C^[CT]CG[GA]G
Bsp1286I     1848  GGGCAC               G[AGT]GC[ACT]^C
Bsp1407I      242  TGTACA               T^GTACA
BspEI         330  TCCGGA               T^CCGGA
BsrFI        4288  ACCGGT               [GA]^CCGG[CT]
BsrGI         242  TGTACA               T^GTACA
BssSI        3935  CACGAG               CACGAG(-5/-1)
BstBI        4446  TTCGAA               TT^CGAA
BstUI        3641  CGCG                 CG^CG
Bsu36I       2041  CCTGAGG              CC^T[ACGT]AGG
CelII        2427  GCTCAGC              GC^T[ACGT]AGC
Cfr10I       4288  ACCGGT               [GA]^CCGG[CT]
DraII         293  GGGACCC              [GA]G^G[ACGT]CC[CT]
DraIII       1356  CACTGAGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I      474  GACAGGTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I       2041  CCTGAGG              CC^T[ACGT]AGG
EcoO109I      293  GGGACCC              [GA]G^G[ACGT]CC[CT]
HgaI         1587  GACGC                GACGC(5/10)
HindIII      1484  AAGCTT               A^AGCTT
HpaI         1992  GTTAAC               GTT^AAC
KpnI         2371  GGTACC               GGTAC^C
MamI         3247  GATTCGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI          330  TCCGGA               T^CCGGA
MvnI         3641  CGCG                 CG^CG
NcoI         2537  CCATGG               C^CATGG
NspV         4446  TTCGAA               TT^CGAA
PacI         3217  TTAATTAA             TTAAT^TAA
PinAI        4288  ACCGGT               A^CCGGT
PpuMI         293  GGGACCC              [GA]G^G[AT]CC[CT]
PstI         3432  CTGCAG               CTGCA^G
SfuI         4446  TTCGAA               TT^CGAA
SpeI         2977  ACTAGT               A^CTAGT
SspBI         242  TGTACA               T^GTACA
XbaI         2817  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AlwNI        2249  CAGTGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             3356  CAGATTCTG           
AseI         1660  ATTAAT               AT^TAAT
             3216  ATTAAT              
AsnI         1660  ATTAAT               AT^TAAT
             3216  ATTAAT              
Asp700I       561  GAAAATGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2809  GAACAAGTTC          
BclI          990  TGATCA               T^GATCA
             2466  TGATCA              
BsaWI         330  TCCGGA               [AT]^CCGG[AT]
             4288  ACCGGT              
BseRI        2852  CTCCTC               GAGGAG(10/8)
             4520  CTCCTC              
BsrDI          14  CATTGC               GCAATG(2/0)
              948  CATTGC              
BstXI        1394  CCAAAACTTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2235  CCAATTAGCTGG        
DraI          676  TTTAAA               TTT^AAA
             3024  TTTAAA              
DsaI         2537  CCATGG               C^C[GA][CT]GG
             3949  CCACGG              
EcoRI        1310  GAATTC               G^AATTC
             4341  GAATTC              
FbaI          990  TGATCA               T^GATCA
             2466  TGATCA              
HapII         331  CCGG                 C^CGG
             4289  CCGG                
HpaII         331  CCGG                 C^CGG
             4289  CCGG                
MaeII        2473  ACGT                 A^CGT
             3014  ACGT                
MslI         2272  CATCATGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             3672  CATAGAAATG          
MspA1I       1283  CAGCTG               C[AC]G^C[GT]G
             4511  CAGCTG              
MspI          331  CCGG                 C^CGG
             4289  CCGG                
NdeI          728  CATATG               CA^TATG
             4495  CATATG              
PshBI        1660  ATTAAT               AT^TAAT
             3216  ATTAAT              
PvuII        1283  CAGCTG               CAG^CTG
             4511  CAGCTG              
SapI          596  GAAGAGC              GCTCTTC(1/4)
              722  GAAGAGC             
SexAI        2169  ACCAGGT              A^CC[AT]GGT
             3807  ACCAGGT             
SmlI          616  CTCAAG               C^T[CT][GA]AG
             3743  CTCAAG              
XmnI          561  GAAAATGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2809  GAACAAGTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   77 enzymes) #
#########################################

AciI        AcsI        AfaI        AflIII      AluI        
AlwI        ApoI        AvaII       BamHI       BanI        
BbsI        BbvI        BfaI        BpuAI       BsaJI       
BsiYI       BslI        BsmAI       BsmFI       BspLU11I    
BsrI        BssKI       BstF5I      BstNI       BstYI       
Cac8I       Cfr13I      DdeI        DpnII       EarI        
EcoNI       EcoRII      EcoT14I     Fnu4HI      FokI        
HaeIII      HincII      HindII      HinfI       HphI        
ItaI        Ksp632I     MaeI        MaeIII      MboI        
MboII       MfeI        MflI        MnlI        MseI        
MunI        MvaI        MwoI        NdeII       NlaIII      
NlaIV       NspI        PleI        RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SspI        
StuI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
XcmI        XhoII       
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