Restriction map for pF1KE0800
 (Linear) (Six-base) MAPSORT of: KIEE0800.seq  Check: 5968  from: 1  to: 4593



KIEE0800.orf

 With 182 enzymes: SgfI * 

                        September 2, 2010 12:21  ..

Acc65I G'GTAC_C

Cuts at:      0    2371    4593 
   Size:       2371    2222
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1531    1949    2579    2939    3644    3752    4593 
   Size:       1531     418     630     360     705     108     841

  Fragments arranged by size: 

                1531     841     705     630     418     360     108
 
AflIII A'CryG_T

Cuts at:      0     643     815     972    1076    2471    4593 
   Size:        643     172     157     104    1395    2122

  Fragments arranged by size: 

                2122    1395     643     172     157     104
 
AgeI A'CCGG_T

Cuts at:      0    4288    4593 
   Size:       4288     305
 
AhdI GACnn_n'nnGTC

Cuts at:      0     479    4593 
   Size:        479    4114
 
AlwNI CAG_nnn'CTG

Cuts at:      0    2254    3361    4593 
   Size:       2254    1107    1232

  Fragments arranged by size: 

                2254    1232    1107
 
ApoI r'AATT_y

Cuts at:      0     526    1189    1310    1714    1780    2121    2319    2899 
   Size:        526     663     121     404      66     341     198     580

Cuts at:   2899    3973    4341    4593 
   Size:       1074     368     252

  Fragments arranged by size: 

                1074     663     580     526     404     368     341     252
                 198     121      66
 
AseI AT'TA_AT

Cuts at:      0    1661    3217    4593 
   Size:       1661    1556    1376

  Fragments arranged by size: 

                1661    1556    1376
 
AvaI C'yCGr_G

Cuts at:      0    4523    4593 
   Size:       4523      70
 
AvrII C'CTAG_G

Cuts at:      0    3111    4593 
   Size:       3111    1482
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    2879    2912    4593 
   Size:       2879      33    1681

  Fragments arranged by size: 

                2879    1681      33
 
BamHI G'GATC_C

Cuts at:      0     742    3252    4329    4593 
   Size:        742    2510    1077     264

  Fragments arranged by size: 

                2510    1077     742     264
 
BanI G'GyrC_C

Cuts at:      0    1849    2371    2836    4291    4593 
   Size:       1849     522     465    1455     302

  Fragments arranged by size: 

                1849    1455     522     465     302
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1537    1591    2022    2255    2851    2955    3512    4163 
   Size:       1537      54     431     233     596     104     557     651

Cuts at:   4163    4258    4265    4593 
   Size:         95       7     328

  Fragments arranged by size: 

                1537     651     596     557     431     328     233     104
                  95      54       7
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1915    4593 
   Size:       1915    2678
 
BclI T'GATC_A

Cuts at:      0     990    2466    4593 
   Size:        990    1476    2127

  Fragments arranged by size: 

                2127    1476     990
 
BglII A'GATC_T

Cuts at:      0    2137    4593 
   Size:       2137    2456
 
BlpI GC'TnA_GC

Cuts at:      0    2428    4593 
   Size:       2428    2165
 
Bme1580I G_kGCm'C

Cuts at:      0    1852    4593 
   Size:       1852    2741
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1248    2582    4216    4593 
   Size:       1248    1334    1634     377

  Fragments arranged by size: 

                1634    1334    1248     377
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1820    4593 
   Size:       1820    2773
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1729    4593 
   Size:       1729    2864
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     601    3728    4593 
   Size:        601    3127     865

  Fragments arranged by size: 

                3127     865     601
 
BsaAI yAC'GTr

Cuts at:      0    3015    4593 
   Size:       3015    1578
 
BsaBI GATnn'nnATC

Cuts at:      0    3251    4593 
   Size:       3251    1342
 
BsaWI w'CCGG_w

Cuts at:      0     330    4288    4593 
   Size:        330    3958     305

  Fragments arranged by size: 

                3958     330     305
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    2843    4511    4593 
   Size:       2843    1668      82

  Fragments arranged by size: 

                2843    1668      82
 
BseYI C'CCAG_C

Cuts at:      0     173     411    3278    4593 
   Size:        173     238    2867    1315

  Fragments arranged by size: 

                2867    1315     238     173
 
BsmI GAATG_Cn'

Cuts at:      0    3122    4593 
   Size:       3122    1471
 
Bsp1286I G_dGCh'C

Cuts at:      0    1852    4593 
   Size:       1852    2741
 
BspEI T'CCGG_A

Cuts at:      0     330    4593 
   Size:        330    4263
 
BsrDI GCAATG_nn'

Cuts at:      0      13     947    4593 
   Size:         13     934    3646

  Fragments arranged by size: 

                3646     934      13
 
BsrFI r'CCGG_y

Cuts at:      0    4288    4593 
   Size:       4288     305
 
BsrGI T'GTAC_A

Cuts at:      0     242    4593 
   Size:        242    4351
 
BssSI C'ACGA_G

Cuts at:      0    3935    4593 
   Size:       3935     658
 
BstBI TT'CG_AA

Cuts at:      0    4447    4593 
   Size:       4447     146
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1401    2242    4593 
   Size:       1401     841    2351

  Fragments arranged by size: 

                2351    1401     841
 
BstYI r'GATC_y

Cuts at:      0     742    2137    3252    4329    4593 
   Size:        742    1395    1115    1077     264

  Fragments arranged by size: 

                1395    1115    1077     742     264
 
Bsu36I CC'TnA_GG

Cuts at:      0    2042    4593 
   Size:       2042    2551
 
BtgI C'CryG_G

Cuts at:      0    2537    3949    4593 
   Size:       2537    1412     644

  Fragments arranged by size: 

                2537    1412     644
 
BtsI GCAGTG_nn'

Cuts at:      0    3912    4164    4593 
   Size:       3912     252     429

  Fragments arranged by size: 

                3912     429     252
 
DraI TTT'AAA

Cuts at:      0     678    3026    4593 
   Size:        678    2348    1567

  Fragments arranged by size: 

                2348    1567     678
 
DraIII CAC_nnn'GTG

Cuts at:      0    1361    4593 
   Size:       1361    3232
 
EarI CTCTTCn'nnn_

Cuts at:      0     591     717    1694    1781    1789    3305    3862    4562 
   Size:        591     126     977      87       8    1516     557     700

Cuts at:   4562    4593 
   Size:         31

  Fragments arranged by size: 

                1516     977     700     591     557     126      87      31
                   8
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     124    4593 
   Size:        124    4469
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1531    1729    1949    2579    2939    3644    3752    4593 
   Size:       1531     198     220     630     360     705     108     841

  Fragments arranged by size: 

                1531     841     705     630     360     220     198     108
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1378    1457    3584    4593 
   Size:       1378      79    2127    1009

  Fragments arranged by size: 

                2127    1378    1009      79
 
EcoO109I rG'GnC_Cy

Cuts at:      0     294    4593 
   Size:        294    4299
 
EcoRI G'AATT_C

Cuts at:      0    1310    4341    4593 
   Size:       1310    3031     252

  Fragments arranged by size: 

                3031    1310     252
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     784    3592    4289    4593 
   Size:        784    2808     697     304

  Fragments arranged by size: 

                2808     784     697     304
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     465     497    1527    1559    1889    1921    1962    1994 
   Size:        465      32    1030      32     330      32      41      32

Cuts at:   1994    2838    2870    3877    3909    4408    4440    4492    4524 
   Size:        844      32    1007      32     499      32      52      32

Cuts at:   4524    4593 
   Size:         69

  Fragments arranged by size: 

                1030    1007     844     499     465     330      69      52
                  41      32      32      32      32      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0      45     942    1994    4593 
   Size:         45     897    1052    2599

  Fragments arranged by size: 

                2599    1052     897      45
 
HindIII A'AGCT_T

Cuts at:      0    1484    4593 
   Size:       1484    3109
 
HpaI GTT'AAC

Cuts at:      0    1994    4593 
   Size:       1994    2599
 
KpnI G_GTAC'C

Cuts at:      0    2375    4593 
   Size:       2375    2218
 
MfeI C'AATT_G

Cuts at:      0     115     323    1177    4593 
   Size:        115     208     854    3416

  Fragments arranged by size: 

                3416     854     208     115
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     815    2928    3026    3552    4593 
   Size:        815    2113      98     526    1041

  Fragments arranged by size: 

                2113    1041     815     526      98
 
MslI CAynn'nnrTG

Cuts at:      0    2276    3676    4593 
   Size:       2276    1400     917

  Fragments arranged by size: 

                2276    1400     917
 
MspA1I CmG'CkG

Cuts at:      0    1285    4513    4593 
   Size:       1285    3228      80

  Fragments arranged by size: 

                3228    1285      80
 
NcoI C'CATG_G

Cuts at:      0    2537    4593 
   Size:       2537    2056
 
NdeI CA'TA_TG

Cuts at:      0     729    4496    4593 
   Size:        729    3767      97

  Fragments arranged by size: 

                3767     729      97
 
NspI r_CATG'y

Cuts at:      0     647     819     976    1080    1175    2990    4593 
   Size:        647     172     157     104      95    1815    1603

  Fragments arranged by size: 

                1815    1603     647     172     157     104      95
 
PacI TTA_AT'TAA

Cuts at:      0    3221    4593 
   Size:       3221    1372
 
PciI A'CATG_T

Cuts at:      0     643     815     972    1076    4593 
   Size:        643     172     157     104    3517

  Fragments arranged by size: 

                3517     643     172     157     104
 
PpuMI rG'GwC_Cy

Cuts at:      0     294    4593 
   Size:        294    4299
 
PstI C_TGCA'G

Cuts at:      0    3436    4593 
   Size:       3436    1157
 
PvuII CAG'CTG

Cuts at:      0    1285    4513    4593 
   Size:       1285    3228      80

  Fragments arranged by size: 

                3228    1285      80
 
SanDI GG'GwC_CC

Cuts at:      0     294    4593 
   Size:        294    4299
 
SapI GCTCTTCn'nnn_

Cuts at:      0     591     717    4593 
   Size:        591     126    3876

  Fragments arranged by size: 

                3876     591     126
 
SexAI A'CCwGG_T

Cuts at:      0    2169    3807    4593 
   Size:       2169    1638     786

  Fragments arranged by size: 

                2169    1638     786
 
SfcI C'TryA_G

Cuts at:      0     213    2868    3432    4593 
   Size:        213    2655     564    1161

  Fragments arranged by size: 

                2655    1161     564     213
 
SmlI C'TyrA_G

Cuts at:      0     616    3743    4593 
   Size:        616    3127     850

  Fragments arranged by size: 

                3127     850     616
 
SpeI A'CTAG_T

Cuts at:      0    2977    4593 
   Size:       2977    1616
 
SspI AAT'ATT

Cuts at:      0    1834    1918    3085    4593 
   Size:       1834      84    1167    1508

  Fragments arranged by size: 

                1834    1508    1167      84
 
StuI AGG'CCT

Cuts at:      0    1415    3265    3770    4593 
   Size:       1415    1850     505     823

  Fragments arranged by size: 

                1850    1415     823     505
 
StyI C'CwwG_G

Cuts at:      0     959    1416    2537    3111    3410    3548    3599    4472 
   Size:        959     457    1121     574     299     138      51     873

Cuts at:   4472    4593 
   Size:        121

  Fragments arranged by size: 

                1121     959     873     574     457     299     138     121
                  51
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1230    2564    4593 
   Size:       1230    1334    2029

  Fragments arranged by size: 

                2029    1334    1230
 
TatI w'GTAC_w

Cuts at:      0     242    1550    4593 
   Size:        242    1308    3043

  Fragments arranged by size: 

                3043    1308     242
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     216     248     312     344    4593 
   Size:        216      32      64      32    4249

  Fragments arranged by size: 

                4249     216      64      32      32
 
XbaI T'CTAG_A

Cuts at:      0    2817    4593 
   Size:       2817    1776
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     531    2307    3494    4593 
   Size:        531    1776    1187    1099

  Fragments arranged by size: 

                1776    1187    1099     531
 
XmnI GAAnn'nnTTC

Cuts at:      0     565    2813    4593 
   Size:        565    2248    1780

  Fragments arranged by size: 

                2248    1780     565
 

 Enzymes that do cut:

   Acc65I     AcuI   AflIII     AgeI     AhdI    AlwNI     ApoI     AseI
     AvaI    AvrII     BaeI    BamHI     BanI     BbsI    BciVI     BclI
    BglII     BlpI Bme1580I     BmrI     BplI     BpmI    BpuEI    BsaAI
    BsaBI    BsaWI    BseRI    BseYI     BsmI Bsp1286I    BspEI    BsrDI
    BsrFI    BsrGI    BssSI    BstBI    BstXI    BstYI   Bsu36I     BtgI
     BtsI     DraI   DraIII     EarI     EciI  Eco57MI    EcoNI EcoO109I
    EcoRI     FalI    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI
     MmeI     MslI   MspA1I     NcoI     NdeI     NspI     PacI     PciI
    PpuMI     PstI    PvuII    SanDI     SapI    SexAI     SfcI     SmlI
     SpeI     SspI     StuI     StyI    TaqII     TatI     TstI     XbaI
     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII     AleI     AlfI
     AloI     ApaI    ApaLI     AscI    AsiSI    BanII     BbeI    BbvCI
     BcgI    BfrBI     BglI    BmgBI     BmtI   Bpu10I     BsaI    BsaHI
    BsaXI     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI    BspHI    BspMI
    BsrBI   BssHII   BstAPI   BstEII  BstZ17I    BtgZI     ClaI    CspCI
     DrdI     EaeI     EagI  EcoICRI    EcoRV     FseI     FspI    FspAI
    HaeII     KasI     MluI     MscI     NaeI     NarI   NgoMIV     NheI
     NotI     NruI     NsiI     PasI    PflMI     PfoI     PmeI     PmlI
     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII
     SacI    SacII     SalI     SbfI     ScaI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SphI     SrfI     SwaI  Tth111I     XhoI
     XmaI     ZraI
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