Restriction map for
pF1KE2491
(Circular) (Six-base) MAPSORT of: pF1KE2491.seq Check: 7147 from: 1 to: 10172
pF1KE2491 10172 bp
With 182 enzymes: SgfI *
September 4, 2015 15:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3756 3756
Size: 10172
AatII G_ACGT'C
Cuts at: 2309 3211 2309
Size: 902 9270
AccI GT'mk_AC
Cuts at: 1661 4664 5621 7210 1661
Size: 3003 957 1589 4623
Fragments arranged by size:
4623 3003 1589 957
Acc65I G'GTAC_C
Cuts at: 7188 7188
Size: 10172
AclI AA'CG_TT
Cuts at: 10012 10012
Size: 10172
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1510 2218 2436 2475 2653 2803 2877 2956 3286
Size: 708 218 39 178 150 74 79 330
Cuts at: 3286 5125 5305 5314 5463 6980 7997 8429 9176
Size: 1839 180 9 149 1517 1017 432 747
Cuts at: 9176 9444 1510
Size: 268 2238
Fragments arranged by size:
2238 1839 1517 1017 747 708 432 330
268 218 180 178 150 149 79 74
39 9
AfeI AGC'GCT
Cuts at: 502 6856 502
Size: 6354 3818
AflIII A'CryG_T
Cuts at: 614 1142 4751 5903 7524 8628 9605 614
Size: 528 3609 1152 1621 1104 977 1181
Fragments arranged by size:
3609 1621 1181 1152 1104 977 528
AgeI A'CCGG_T
Cuts at: 3950 8560 3950
Size: 4610 5562
AhdI GACnn_n'nnGTC
Cuts at: 1815 3207 1815
Size: 1392 8780
AleI CACnn'nnGTG
Cuts at: 1175 5080 5332 1175
Size: 3905 252 6015
Fragments arranged by size:
6015 3905 252
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3136 3136
Size: 10172
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 955 987 2965 2997 955
Size: 32 1978 32 8130
Fragments arranged by size:
8130 1978 32 32
AlwNI CAG_nnn'CTG
Cuts at: 1819 1897 2714 3018 3857 4587 6226 7477 9044
Size: 78 817 304 839 730 1639 1251 1567
Cuts at: 9044 1819
Size: 2947
Fragments arranged by size:
2947 1639 1567 1251 839 817 730 304
78
ApaI G_GGCC'C
Cuts at: 1875 3508 3671 3927 4347 4492 5770 6625 1875
Size: 1633 163 256 420 145 1278 855 5422
Fragments arranged by size:
5422 1633 1278 855 420 256 163 145
ApaLI G'TGCA_C
Cuts at: 1648 1822 3342 4947 5777 6010 6163 8942 1648
Size: 174 1520 1605 830 233 153 2779 2878
Fragments arranged by size:
2878 2779 1605 1520 830 233 174 153
ApoI r'AATT_y
Cuts at: 2293 7176 9525 9781 2293
Size: 4883 2349 256 2684
Fragments arranged by size:
4883 2684 2349 256
AscI GG'CGCG_CC
Cuts at: 155 1769 155
Size: 1614 8558
AseI AT'TA_AT
Cuts at: 20 20
Size: 10172
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 10172
AvaI C'yCGr_G
Cuts at: 205 796 1152 1606 1729 1874 2412 2560 3664
Size: 591 356 454 123 145 538 148 1104
Cuts at: 3664 4347 5770 6107 6925 7192 205
Size: 683 1423 337 818 267 3185
Fragments arranged by size:
3185 1423 1104 818 683 591 538 454
356 337 267 148 145 123
AvrII C'CTAG_G
Cuts at: 6199 6199
Size: 10172
BamHI G'GATC_C
Cuts at: 758 6213 7197 758
Size: 5455 984 3733
Fragments arranged by size:
5455 3733 984
BanI G'GyrC_C
Cuts at: 144 210 295 383 1177 1610 1738 1933 2032
Size: 66 85 88 794 433 128 195 99
Cuts at: 2032 2813 3299 3514 3902 4270 4844 5827 5919
Size: 781 486 215 388 368 574 983 92
Cuts at: 5919 5931 5983 6823 7188 7602 7850 7885 144
Size: 12 52 840 365 414 248 35 2431
Fragments arranged by size:
2431 983 840 794 781 574 486 433
414 388 368 365 248 215 195 128
99 92 88 85 66 52 35 12
BanII G_rGCy'C
Cuts at: 205 245 268 1327 1875 2249 3379 3508 3671
Size: 40 23 1059 548 374 1130 129 163
Cuts at: 3671 3887 3927 4347 4492 5770 5998 6089 6268
Size: 216 40 420 145 1278 228 91 179
Cuts at: 6268 6458 6625 6638 6673 6715 6809 6820 7186
Size: 190 167 13 35 42 94 11 366
Cuts at: 7186 8216 205
Size: 1030 2161
Fragments arranged by size:
2161 1278 1130 1059 1030 548 420 374
366 228 216 190 179 167 163 145
129 94 91 42 40 40 35 23
13 11
BbeI G_GCGC'C
Cuts at: 214 1742 2817 3518 4274 4848 5831 5935 6827
Size: 1528 1075 701 756 574 983 104 892
Cuts at: 6827 7606 7854 214
Size: 779 248 2532
Fragments arranged by size:
2532 1528 1075 983 892 779 756 701
574 248 104
BbsI GAAGACnn'nnnn_
Cuts at: 336 819 2842 2944 4005 4077 5064 5082 5447
Size: 483 2023 102 1061 72 987 18 365
Cuts at: 5447 336
Size: 5061
Fragments arranged by size:
5061 2023 1061 987 483 365 102 72
18
BbvCI CC'TCA_GC
Cuts at: 3644 4090 7125 3644
Size: 446 3035 6691
Fragments arranged by size:
6691 3035 446
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 6697 6731 6697
Size: 34 10138
BciVI GTATCCnnnnn_n'
Cuts at: 774 8070 8831 10172 774
Size: 7296 761 1341 774
Fragments arranged by size:
7296 1341 774 761
BclI T'GATC_A
Cuts at: 1229 1337 3996 7692 1229
Size: 108 2659 3696 3709
Fragments arranged by size:
3709 3696 2659 108
BglI GCCn_nnn'nGGC
Cuts at: 334 1628 1879 2657 3512 3720 334
Size: 1294 251 778 855 208 6786
Fragments arranged by size:
6786 1294 855 778 251 208
BglII A'GATC_T
Cuts at: 7687 9724 7687
Size: 2037 8135
BlpI GC'TnA_GC
Cuts at: 2248 2530 6881 7284 2248
Size: 282 4351 403 5136
Fragments arranged by size:
5136 4351 403 282
Bme1580I G_kGCm'C
Cuts at: 149 1422 1613 1652 1826 1875 1936 2045 2153
Size: 1273 191 39 174 49 61 109 108
Cuts at: 2153 2182 2226 2397 3302 3346 3498 3508 3561
Size: 29 44 171 905 44 152 10 53
Cuts at: 3561 3671 3927 4347 4492 4951 5770 5781 6014
Size: 110 256 420 145 459 819 11 233
Cuts at: 6014 6115 6167 6625 7013 7797 7890 8946 149
Size: 101 52 458 388 784 93 1056 1375
Fragments arranged by size:
1375 1273 1056 905 819 784 459 458
420 388 256 233 191 174 171 152
145 110 109 108 101 93 61 53
52 49 44 44 39 29 11 10
BmrI ACTGGGnnnn_n'
Cuts at: 1577 2122 3460 3503 3891 5643 6122 6929 7089
Size: 545 1338 43 388 1752 479 807 160
Cuts at: 7089 7384 7465 7800 9944 1577
Size: 295 81 335 2144 1805
Fragments arranged by size:
2144 1805 1752 1338 807 545 479 388
335 295 160 81 43
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 510 8539 510
Size: 8029 2143
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 535 2248 2602 2641 3235 3325 3519 3540 3561
Size: 1713 354 39 594 90 194 21 21
Cuts at: 3561 3901 4066 5221 5449 5716 5773 6028 6330
Size: 340 165 1155 228 267 57 255 302
Cuts at: 6330 6823 7126 535
Size: 493 303 3581
Fragments arranged by size:
3581 1713 1155 594 493 354 340 303
302 267 255 228 194 165 90 57
39 21 21
Bpu10I CC'TnA_GC
Cuts at: 3644 4090 4387 5461 5593 6527 6819 7125 3644
Size: 446 297 1074 132 934 292 306 6691
Fragments arranged by size:
6691 1074 934 446 306 297 292 132
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3553 4339 5038 7348 8227 8719 9017 3553
Size: 786 699 2310 879 492 298 4708
Fragments arranged by size:
4708 2310 879 786 699 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3449 5962 6935 9876 3449
Size: 2513 973 2941 3745
Fragments arranged by size:
3745 2941 2513 973
BsaAI yAC'GTr
Cuts at: 420 617 1497 4002 7525 420
Size: 197 880 2505 3523 3067
Fragments arranged by size:
3523 3067 2505 880 197
BsaBI GATnn'nnATC
Cuts at: 6 562 1216 2512 7686 9723 6
Size: 556 654 1296 5174 2037 455
Fragments arranged by size:
5174 2037 1296 654 556 455
BsaHI Gr'CG_yC
Cuts at: 190 211 527 851 1274 1739 2285 2306 2814
Size: 21 316 324 423 465 546 21 508
Cuts at: 2814 3208 3515 4271 4763 4845 5738 5828 5932
Size: 394 307 756 492 82 893 90 104
Cuts at: 5932 6824 7603 7851 8553 10049 190
Size: 892 779 248 702 1496 313
Fragments arranged by size:
1496 893 892 779 756 702 546 508
492 465 423 394 324 316 313 307
248 104 90 82 21 21
BsaWI w'CCGG_w
Cuts at: 2666 2947 3950 6477 7361 7882 8560 8834 8981
Size: 281 1003 2527 884 521 678 274 147
Cuts at: 8981 9392 2666
Size: 411 3446
Fragments arranged by size:
3446 2527 1003 884 678 521 411 281
274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 220 250 652 682 955 985 2942 2965 2972
Size: 30 402 30 273 30 1957 23 7
Cuts at: 2972 2995 3922 3952 4699 4729 6328 6358 6781
Size: 23 927 30 747 30 1599 30 423
Cuts at: 6781 6811 220
Size: 30 3581
Fragments arranged by size:
3581 1957 1599 927 747 423 402 273
30 30 30 30 30 30 30 23
23 7
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 319 967 972 976 2578 3060 3238 4825 4843
Size: 648 5 4 1602 482 178 1587 18
Cuts at: 4843 4869 5218 5713 6388 6418 319
Size: 26 349 495 675 30 4073
Fragments arranged by size:
4073 1602 1587 675 648 495 482 349
178 30 26 18 5 4
BseYI C'CCAG_C
Cuts at: 291 1061 1244 1929 2164 2172 2324 2384 3400
Size: 770 183 685 235 8 152 60 1016
Cuts at: 3400 3562 3781 4544 4876 4889 5403 6069 6393
Size: 162 219 763 332 13 514 666 324
Cuts at: 6393 6585 6857 6898 7597 8355 8932 9428 291
Size: 192 272 41 699 758 577 496 1035
Fragments arranged by size:
1035 1016 770 763 758 699 685 666
577 514 496 332 324 272 235 219
192 183 162 152 60 41 13 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1035 1088 2199 2847 4474 4699 5084 5454 7172
Size: 53 1111 648 1627 225 385 370 1718
Cuts at: 7172 1035
Size: 4035
Fragments arranged by size:
4035 1718 1627 1111 648 385 370 225
53
BsiEI CG_ry'CG
Cuts at: 104 275 373 937 1029 3724 3728 3756 6355
Size: 171 98 564 92 2695 4 28 2599
Cuts at: 6355 7714 7760 8968 104
Size: 1359 46 1208 1308
Fragments arranged by size:
2695 2599 1359 1308 1208 564 171 98
92 46 28 4
BsiHKAI G_wGCw'C
Cuts at: 245 419 1015 1652 1826 2249 3125 3346 3379
Size: 174 596 637 174 423 876 221 33
Cuts at: 3379 4178 4748 4870 4951 5021 5572 5684 5781
Size: 799 570 122 81 70 551 112 97
Cuts at: 5781 6014 6167 6887 7186 7964 8154 8946 245
Size: 233 153 720 299 778 190 792 1471
Fragments arranged by size:
1471 876 799 792 778 720 637 596
570 551 423 299 233 221 190 174
174 153 122 112 97 81 70 33
BsmBI CGTCTCn'nnnn_
Cuts at: 1372 3966 4101 4363 6348 1372
Size: 2594 135 262 1985 5196
Fragments arranged by size:
5196 2594 1985 262 135
Bsp1286I G_dGCh'C
Cuts at: 149 205 245 268 419 1015 1327 1422 1613
Size: 56 40 23 151 596 312 95 191
Cuts at: 1613 1652 1826 1875 1936 2045 2153 2182 2226
Size: 39 174 49 61 109 108 29 44
Cuts at: 2226 2249 2397 3125 3302 3346 3379 3498 3508
Size: 23 148 728 177 44 33 119 10
Cuts at: 3508 3561 3671 3887 3927 4178 4347 4492 4748
Size: 53 110 216 40 251 169 145 256
Cuts at: 4748 4870 4951 5021 5572 5684 5770 5781 5998
Size: 122 81 70 551 112 86 11 217
Cuts at: 5998 6014 6089 6115 6167 6268 6458 6625 6638
Size: 16 75 26 52 101 190 167 13
Cuts at: 6638 6673 6715 6809 6820 6887 7013 7186 7797
Size: 35 42 94 11 67 126 173 611
Cuts at: 7797 7890 7964 8154 8216 8946 149
Size: 93 74 190 62 730 1375
Fragments arranged by size:
1375 730 728 611 596 551 312 256
251 217 216 191 190 190 177 174
173 169 167 151 148 145 126 122
119 112 110 109 108 101 95 94
93 86 81 75 74 70 67 62
61 56 53 52 49 44 44 42
40 40 39 35 33 29 26 23
23 16 13 11 11 10
BspEI T'CCGG_A
Cuts at: 2666 9392 2666
Size: 6726 3446
BspHI T'CATG_A
Cuts at: 1334 2504 3101 5546 5684 9348 1334
Size: 1170 597 2445 138 3664 2158
Fragments arranged by size:
3664 2445 2158 1170 597 138
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3140 3756 4193 4211 5609 6509 7222 7436 7738
Size: 616 437 18 1398 900 713 214 302
Cuts at: 7738 8119 3140
Size: 381 5193
Fragments arranged by size:
5193 1398 900 713 616 437 381 302
214 18
BsrBI CCG'CTC
Cuts at: 50 231 1151 4080 4931 6080 6122 6495 6640
Size: 181 920 2929 851 1149 42 373 145
Cuts at: 6640 8464 8518 10002 10167 50
Size: 1824 54 1484 165 55
Fragments arranged by size:
2929 1824 1484 1149 920 851 373 181
165 145 55 54 42
BsrDI GCAATG_nn'
Cuts at: 5270 8084 5270
Size: 2814 7358
BsrFI r'CCGG_y
Cuts at: 2017 3527 3723 3950 4790 4895 5604 5980 6137
Size: 1510 196 227 840 105 709 376 157
Cuts at: 6137 6249 6350 6354 6484 8170 8351 8560 2017
Size: 112 101 4 130 1686 181 209 3629
Fragments arranged by size:
3629 1686 1510 840 709 376 227 209
196 181 157 130 112 105 101 4
BsrGI T'GTAC_A
Cuts at: 3047 3047
Size: 10172
BssHII G'CGCG_C
Cuts at: 155 1769 5313 6649 155
Size: 1614 3544 1336 3678
Fragments arranged by size:
3678 3544 1614 1336
BssSI C'ACGA_G
Cuts at: 2821 3148 8443 8801 2821
Size: 327 5295 358 4192
Fragments arranged by size:
5295 4192 358 327
BstAPI GCAn_nnn'nTGC
Cuts at: 383 1049 1115 1897 3876 4677 7549 383
Size: 666 66 782 1979 801 2872 3006
Fragments arranged by size:
3006 2872 1979 801 782 666 66
BstBI TT'CG_AA
Cuts at: 8533 8533
Size: 10172
BstEII G'GTnAC_C
Cuts at: 2317 2445 6252 7051 2317
Size: 128 3807 799 5438
Fragments arranged by size:
5438 3807 799 128
BstXI CCAn_nnnn'nTGG
Cuts at: 1929 2125 3150 4044 4125 4281 5013 5040 1929
Size: 196 1025 894 81 156 732 27 7061
Fragments arranged by size:
7061 1025 894 732 196 156 81 27
BstYI r'GATC_y
Cuts at: 1 758 2084 3014 3184 4277 6047 6213 7197
Size: 757 1326 930 170 1093 1770 166 984
Cuts at: 7197 7664 7687 8022 8268 9280 9366 9378 9724
Size: 467 23 335 246 1012 86 12 346
Cuts at: 9724 1
Size: 449
Fragments arranged by size:
1770 1326 1093 1012 984 930 757 467
449 346 335 246 170 166 86 23
12
Bsu36I CC'TnA_GG
Cuts at: 1912 1962 6626 1912
Size: 50 4664 5458
Fragments arranged by size:
5458 4664 50
BtgI C'CryG_G
Cuts at: 338 406 1704 1797 2280 2496 2840 3576 3709
Size: 68 1298 93 483 216 344 736 133
Cuts at: 3709 4253 4655 5012 5522 5915 6292 6459 6728
Size: 544 402 357 510 393 377 167 269
Cuts at: 6728 7163 338
Size: 435 3347
Fragments arranged by size:
3347 1298 736 544 510 483 435 402
393 377 357 344 269 216 167 133
93 68
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3188 4023 8122 8303 8466 3188
Size: 835 4099 181 163 4894
Fragments arranged by size:
4894 4099 835 181 163
BtsI GCAGTG_nn'
Cuts at: 1895 3682 5442 5586 6336 6571 7415 1895
Size: 1787 1760 144 750 235 844 4652
Fragments arranged by size:
4652 1787 1760 844 750 235 144
DraI TTT'AAA
Cuts at: 7171 7171
Size: 10172
DraIII CAC_nnn'GTG
Cuts at: 684 2347 6969 684
Size: 1663 4622 3887
Fragments arranged by size:
4622 3887 1663
DrdI GACnn_nn'nnGTC
Cuts at: 6406 6928 7878 8736 6406
Size: 522 950 858 7842
Fragments arranged by size:
7842 950 858 522
EaeI y'GGCC_r
Cuts at: 272 335 370 934 1026 1448 1919 2096 2127
Size: 63 35 564 92 422 471 177 31
Cuts at: 2127 2277 2510 3140 3281 3470 3579 3721 3725
Size: 150 233 630 141 189 109 142 4
Cuts at: 3725 4115 4250 5165 5675 6352 6482 7757 7931
Size: 390 135 915 510 677 130 1275 174
Cuts at: 7931 8322 8349 9694 272
Size: 391 27 1345 750
Fragments arranged by size:
1345 1275 915 750 677 630 564 510
471 422 391 390 233 189 177 174
150 142 141 135 130 109 92 63
35 31 27 4
EagI C'GGCC_G
Cuts at: 272 370 934 1026 3721 3725 6352 7757 272
Size: 98 564 92 2695 4 2627 1405 2687
Fragments arranged by size:
2695 2687 2627 1405 564 98 92 4
EarI CTCTTCn'nnn_
Cuts at: 1475 2194 2884 3130 3940 4465 4975 6999 7060
Size: 719 690 246 810 525 510 2024 61
Cuts at: 7060 8195 8405 1475
Size: 1135 210 3242
Fragments arranged by size:
3242 2024 1135 810 719 690 525 510
246 210 61
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 241 2749 3181 3853 4144 4822 6008 6383 6417
Size: 2508 432 672 291 678 1186 375 34
Cuts at: 6417 6595 8380 8690 8836 9982 241
Size: 178 1785 310 146 1146 431
Fragments arranged by size:
2508 1785 1186 1146 678 672 432 431
375 310 291 178 146 34
EcoICRI GAG'CTC
Cuts at: 243 2247 3377 7184 243
Size: 2004 1130 3807 3231
Fragments arranged by size:
3807 3231 2004 1130
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 535 1510 2218 2248 2436 2475 2602 2641 2653
Size: 975 708 30 188 39 127 39 12
Cuts at: 2653 2803 2877 2956 3235 3286 3325 3519 3540
Size: 150 74 79 279 51 39 194 21
Cuts at: 3540 3561 3901 4066 5125 5221 5305 5314 5449
Size: 21 340 165 1059 96 84 9 135
Cuts at: 5449 5463 5716 5773 6028 6330 6823 6980 7126
Size: 14 253 57 255 302 493 157 146
Cuts at: 7126 7997 8429 9176 9444 535
Size: 871 432 747 268 1263
Fragments arranged by size:
1263 1059 975 871 747 708 493 432
340 302 279 268 255 253 194 188
165 157 150 146 135 127 96 84
79 74 57 51 39 39 39 30
21 21 14 12 9
EcoNI CCTnn'n_nnAGG
Cuts at: 906 1790 3022 3151 4579 7139 906
Size: 884 1232 129 1428 2560 3939
Fragments arranged by size:
3939 2560 1428 1232 884 129
EcoO109I rG'GnC_Cy
Cuts at: 1603 1871 2121 2232 2328 3441 3504 3505 3668
Size: 268 250 111 96 1113 63 1 163
Cuts at: 3668 3924 4344 4488 4489 4584 5049 5457 5766
Size: 256 420 144 1 95 465 408 309
Cuts at: 5766 5767 6498 6621 6622 6764 6834 6930 7118
Size: 1 731 123 1 142 70 96 188
Cuts at: 7118 7311 1603
Size: 193 4464
Fragments arranged by size:
4464 1113 731 465 420 408 309 268
256 250 193 188 163 144 142 123
111 96 96 95 70 63 1 1
1 1
EcoRI G'AATT_C
Cuts at: 2293 7176 2293
Size: 4883 5289
EcoRV GAT'ATC
Cuts at: 4379 4379
Size: 10172
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4581 6342 8191 4581
Size: 1761 1849 6562
Fragments arranged by size:
6562 1849 1761
FseI GG_CCGG'CC
Cuts at: 3727 3727
Size: 10172
FspI TGC'GCA
Cuts at: 1042 2758 3799 6505 7953 1042
Size: 1716 1041 2706 1448 3261
Fragments arranged by size:
3261 2706 1716 1448 1041
HaeII r_GCGC'y
Cuts at: 214 504 1103 1623 1742 2817 3518 3534 3576
Size: 290 599 520 119 1075 701 16 42
Cuts at: 3576 4274 4848 4896 5222 5831 5935 6827 6858
Size: 698 574 48 326 609 104 892 31
Cuts at: 6858 7606 7854 8876 9855 214
Size: 748 248 1022 979 531
Fragments arranged by size:
1075 1022 979 892 748 701 698 609
599 574 531 520 326 290 248 119
104 48 42 31 16
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 220 252 1264 1296 1801 1833 2562 2594 3193
Size: 32 1012 32 505 32 729 32 599
Cuts at: 3193 3225 3826 3858 3981 4013 6697 6729 8014
Size: 32 601 32 123 32 2684 32 1285
Cuts at: 8014 8044 8046 8076 220
Size: 30 2 30 2316
Fragments arranged by size:
2684 2316 1285 1012 729 601 599 505
123 32 32 32 32 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 2523 4665 4995 7211 2523
Size: 2142 330 2216 5484
Fragments arranged by size:
5484 2216 2142 330
KasI G'GCGC_C
Cuts at: 210 1738 2813 3514 4270 4844 5827 5931 6823
Size: 1528 1075 701 756 574 983 104 892
Cuts at: 6823 7602 7850 210
Size: 779 248 2532
Fragments arranged by size:
2532 1528 1075 983 892 779 756 701
574 248 104
KpnI G_GTAC'C
Cuts at: 7192 7192
Size: 10172
MluI A'CGCG_T
Cuts at: 9605 9605
Size: 10172
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 650 1187 1457 3212 8843 9027 650
Size: 537 270 1755 5631 184 1795
Fragments arranged by size:
5631 1795 1755 537 270 184
MscI TGG'CCA
Cuts at: 1450 1921 2129 2512 3142 4252 5167 7933 1450
Size: 471 208 383 630 1110 915 2766 3689
Fragments arranged by size:
3689 2766 1110 915 630 471 383 208
MslI CAynn'nnrTG
Cuts at: 568 643 1175 1318 2506 2539 2608 2698 3148
Size: 75 532 143 1188 33 69 90 450
Cuts at: 3148 3358 4123 4246 4756 5005 5011 5080 5260
Size: 210 765 123 510 249 6 69 180
Cuts at: 5260 5332 6685 8161 8288 568
Size: 72 1353 1476 127 2452
Fragments arranged by size:
2452 1476 1353 1188 765 532 510 450
249 210 180 143 127 123 90 75
72 69 69 33 6
MspA1I CmG'CkG
Cuts at: 380 884 1389 1401 1416 1472 1550 1562 1799
Size: 504 505 12 15 56 78 12 237
Cuts at: 1799 1903 1976 2176 2252 2324 2388 2456 3349
Size: 104 73 200 76 72 64 68 893
Cuts at: 3349 3539 3578 3854 4196 4544 5157 5949 6593
Size: 190 39 276 342 348 613 792 644
Cuts at: 6593 6730 6918 7283 7597 7957 8970 9215 380
Size: 137 188 365 314 360 1013 245 1337
Fragments arranged by size:
1337 1013 893 792 644 613 505 504
365 360 348 342 314 276 245 237
200 190 188 137 104 78 76 73
72 68 64 56 39 15 12 12
NaeI GCC'GGC
Cuts at: 3529 3725 4792 4897 5606 5982 6139 6352 6356
Size: 196 1067 105 709 376 157 213 4
Cuts at: 6356 6486 8353 3529
Size: 130 1867 5348
Fragments arranged by size:
5348 1867 1067 709 376 213 196 157
130 105 4
NarI GG'CG_CC
Cuts at: 211 1739 2814 3515 4271 4845 5828 5932 6824
Size: 1528 1075 701 756 574 983 104 892
Cuts at: 6824 7603 7851 211
Size: 779 248 2532
Fragments arranged by size:
2532 1528 1075 983 892 779 756 701
574 248 104
NcoI C'CATG_G
Cuts at: 406 1704 2840 4253 5012 6459 406
Size: 1298 1136 1413 759 1447 4119
Fragments arranged by size:
4119 1447 1413 1298 1136 759
NgoMIV G'CCGG_C
Cuts at: 3527 3723 4790 4895 5604 5980 6137 6350 6354
Size: 196 1067 105 709 376 157 213 4
Cuts at: 6354 6484 8351 3527
Size: 130 1867 5348
Fragments arranged by size:
5348 1867 1067 709 376 213 196 157
130 105 4
NotI GC'GGCC_GC
Cuts at: 370 370
Size: 10172
NspI r_CATG'y
Cuts at: 429 465 756 1005 1047 1888 2095 2613 2910
Size: 36 291 249 42 841 207 518 297
Cuts at: 2910 4755 5313 5907 7225 7725 8632 429
Size: 1845 558 594 1318 500 907 1969
Fragments arranged by size:
1969 1845 1318 907 841 594 558 518
500 297 291 249 207 42 36
PasI CC'CwG_GG
Cuts at: 150 456 1056 3500 4155 6258 7141 150
Size: 306 600 2444 655 2103 883 3181
Fragments arranged by size:
3181 2444 2103 883 655 600 306
PciI A'CATG_T
Cuts at: 4751 5903 8628 4751
Size: 1152 2725 6295
Fragments arranged by size:
6295 2725 1152
PflMI CCAn_nnn'nTGG
Cuts at: 2823 7147 2823
Size: 4324 5848
PfoI T'CCnGG_A
Cuts at: 512 890 3386 5872 6536 512
Size: 378 2496 2486 664 4148
Fragments arranged by size:
4148 2496 2486 664 378
PmeI GTTT'AAAC
Cuts at: 7171 7171
Size: 10172
PmlI CAC'GTG
Cuts at: 420 617 4002 420
Size: 197 3385 6590
Fragments arranged by size:
6590 3385 197
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 955 987 2965 2997 9311 9343 955
Size: 32 1978 32 6314 32 1784
Fragments arranged by size:
6314 1978 1784 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 1603 2232 2328 3441 6498 6764 6834 6930 7118
Size: 629 96 1113 3057 266 70 96 188
Cuts at: 7118 1603
Size: 4657
Fragments arranged by size:
4657 3057 1113 629 266 188 96 96
70
PshAI GACnn'nnGTC
Cuts at: 3211 3211
Size: 10172
PspOMI G'GGCC_C
Cuts at: 1871 3504 3667 3923 4343 4488 5766 6621 1871
Size: 1633 163 256 420 145 1278 855 5422
Fragments arranged by size:
5422 1633 1278 855 420 256 163 145
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1120 1152 4621 4653 1120
Size: 32 3469 32 6639
Fragments arranged by size:
6639 3469 32 32
PstI C_TGCA'G
Cuts at: 4190 5954 6151 6222 6275 6843 7219 7904 4190
Size: 1764 197 71 53 568 376 685 6458
Fragments arranged by size:
6458 1764 685 568 376 197 71 53
PvuI CG_AT'CG
Cuts at: 104 3756 104
Size: 3652 6520
PvuII CAG'CTG
Cuts at: 380 1401 1472 3349 3539 3854 4196 5157 7597
Size: 1021 71 1877 190 315 342 961 2440
Cuts at: 7597 7957 380
Size: 360 2595
Fragments arranged by size:
2595 2440 1877 1021 961 360 342 315
190 71
RsrII CG'GwC_CG
Cuts at: 8367 8367
Size: 10172
SacI G_AGCT'C
Cuts at: 245 2249 3379 7186 245
Size: 2004 1130 3807 3231
Fragments arranged by size:
3807 3231 2004 1130
SacII CC_GC'GG
Cuts at: 1800 3579 6731 1800
Size: 1779 3152 5241
Fragments arranged by size:
5241 3152 1779
SalI G'TCGA_C
Cuts at: 4663 7209 4663
Size: 2546 7626
SanDI GG'GwC_CC
Cuts at: 1603 2328 3441 6834 6930 7118 1603
Size: 725 1113 3393 96 188 4657
Fragments arranged by size:
4657 3393 1113 725 188 96
SapI GCTCTTCn'nnn_
Cuts at: 1475 2194 2884 3130 4465 8195 8405 1475
Size: 719 690 246 1335 3730 210 3242
Fragments arranged by size:
3730 3242 1335 719 690 246 210
SbfI CC_TGCA'GG
Cuts at: 7219 7219
Size: 10172
ScaI AGT'ACT
Cuts at: 664 1135 2488 5059 9689 664
Size: 471 1353 2571 4630 1147
Fragments arranged by size:
4630 2571 1353 1147 471
SexAI A'CCwGG_T
Cuts at: 1655 5846 1655
Size: 4191 5981
SfcI C'TryA_G
Cuts at: 33 4186 5950 6147 6218 6271 6513 6839 7215
Size: 4153 1764 197 71 53 242 326 376
Cuts at: 7215 7900 8893 9084 33
Size: 685 993 191 1121
Fragments arranged by size:
4153 1764 1121 993 685 376 326 242
197 191 71 53
SfiI GGCCn_nnn'nGGCC
Cuts at: 334 3720 334
Size: 3386 6786
SfoI GGC'GCC
Cuts at: 212 1740 2815 3516 4272 4846 5829 5933 6825
Size: 1528 1075 701 756 574 983 104 892
Cuts at: 6825 7604 7852 212
Size: 779 248 2532
Fragments arranged by size:
2532 1528 1075 983 892 779 756 701
574 248 104
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 10172
SmaI CCC'GGG
Cuts at: 207 1608 1876 2414 3666 4349 5772 7194 207
Size: 1401 268 538 1252 683 1423 1422 3185
Fragments arranged by size:
3185 1423 1422 1401 1252 683 538 268
SmlI C'TyrA_G
Cuts at: 3568 4354 5053 7327 8242 8734 8996 3568
Size: 786 699 2274 915 492 262 4744
Fragments arranged by size:
4744 2274 915 786 699 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 10172
SphI G_CATG'C
Cuts at: 429 756 1005 1047 2910 5313 7225 7725 429
Size: 327 249 42 1863 2403 1912 500 2876
Fragments arranged by size:
2876 2403 1912 1863 500 327 249 42
SrfI GCCC'GGGC
Cuts at: 3666 3666
Size: 10172
StuI AGG'CCT
Cuts at: 520 1069 3919 4053 5802 6385 6616 6868 520
Size: 549 2850 134 1749 583 231 252 3824
Fragments arranged by size:
3824 2850 1749 583 549 252 231 134
StyI C'CwwG_G
Cuts at: 329 406 578 1704 2003 2840 4253 4514 4613
Size: 77 172 1126 299 837 1413 261 99
Cuts at: 4613 5012 5990 6176 6199 6459 6845 7306 7660
Size: 399 978 186 23 260 386 461 354
Cuts at: 7660 329
Size: 2841
Fragments arranged by size:
2841 1413 1126 978 837 461 399 386
354 299 261 260 186 172 99 77
23
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 127 283 346 598 1236 3201 4273 4438 4536
Size: 156 63 252 638 1965 1072 165 98
Cuts at: 4536 4614 4648 6634 7755 7888 8347 8556 127
Size: 78 34 1986 1121 133 459 209 1743
Fragments arranged by size:
1986 1965 1743 1121 1072 638 459 252
209 165 156 133 98 78 63 34
TatI w'GTAC_w
Cuts at: 662 1133 1259 2486 3047 4559 4706 5057 9687
Size: 471 126 1227 561 1512 147 351 4630
Cuts at: 9687 662
Size: 1147
Fragments arranged by size:
4630 1512 1227 1147 561 471 351 147
126
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 472 504 668 700 2942 2974 6085 6117 6157
Size: 32 164 32 2242 32 3111 32 40
Cuts at: 6157 6189 6198 6230 6328 6360 6781 6813 9689
Size: 32 9 32 98 32 421 32 2876
Cuts at: 9689 9721 472
Size: 32 923
Fragments arranged by size:
3111 2876 2242 923 421 164 98 40
32 32 32 32 32 32 32 32
32 9
Tth111I GACn'n_nGTC
Cuts at: 136 4243 6768 7969 136
Size: 4107 2525 1201 2339
Fragments arranged by size:
4107 2525 2339 1201
XbaI T'CTAG_A
Cuts at: 7203 7203
Size: 10172
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 862 1702 1914 2847 3300 7011 7667 862
Size: 840 212 933 453 3711 656 3367
Fragments arranged by size:
3711 3367 933 840 656 453 212
XmaI C'CCGG_G
Cuts at: 205 1606 1874 2412 3664 4347 5770 7192 205
Size: 1401 268 538 1252 683 1423 1422 3185
Fragments arranged by size:
3185 1423 1422 1401 1252 683 538 268
XmnI GAAnn'nnTTC
Cuts at: 970 2974 3952 970
Size: 2004 978 7190
Fragments arranged by size:
7190 2004 978
ZraI GAC'GTC
Cuts at: 2307 3209 2307
Size: 902 9270
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AscI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PspOMI PsrI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SmaI SmlI SpeI SphI SrfI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI ZraI
Enzymes that do not cut:
AflII BaeI BfrBI BmgBI BmtI BsiWI BsmI BstZ17I
ClaI CspCI FspAI HindIII HpaI MfeI NdeI NheI
NruI NsiI PacI PsiI PspXI SgrAI SnaBI SspI
SwaI XhoI