################################## # NO CUT ENZYMES ( 102 enzymes) # ################################## AatII Acc65I AccI AclI AcyI AflII AgeI AhdI Aor51HI AscI AseI AsnI Asp718I AspEI AviII AvrII BalI BamHI BbeI BbrPI BfrI BglII BlnI BpmI BsaBI BsaHI BsiWI BspDI BspMI BsrBI BsrDI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstYI BstZ17I ClaI CpoI DraIII DrdI Eam1105I Eco47III EcoO65I EcoRI EcoRV EcoT22I FseI FspI HaeII Hin1I HincII HindII HpaI KasI KpnI MamI MfeI MflI MluI MluNI MscI MunI NarI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SalI SapI SbfI ScaI SexAI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I StuI SwaI XbaI XcmI XhoI XhoII ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccIII 134 TCCGGA T^CCGGA AflIII 1454 ACATGT A^C[GA][CT]GT AlwNI 1751 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 478 CCTAAGG CC^T[ACGT]AGG ApaI 524 GGGCCC GGGCC^C AspI 1320 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC BbvCI 341 CCTCAGC CCTCAGC(-5/-2) BciVI 924 GGATAC GTATCC(6/5) BclI 1797 TGATCA T^GATCA BlpI 409 GCTCAGC GC^T[ACGT]AGC Bpu1102I 409 GCTCAGC GC^T[ACGT]AGC BsaAI 1195 TACGTG [CT]AC^GT[GA] BsaI 514 GGTCTC GGTCTC(1/5) BsaWI 134 TCCGGA [AT]^CCGG[AT] BseAI 134 TCCGGA T^CCGGA BsgI 403 CTGCAC GTGCAG(16/14) BsiEI 329 CGGCCG CG[GA][CT]^CG BsmBI 57 CGTCTC CGTCTC(1/5) Bsp1407I 1483 TGTACA T^GTACA BspEI 134 TCCGGA T^CCGGA BspHI 2115 TCATGA T^CATGA BspLU11I 1454 ACATGT A^CATGT BsrGI 1483 TGTACA T^GTACA BssSI 992 CACGAG CACGAG(-5/-1) BstXI 1059 CCAGCCTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 478 CCTAAGG CC^T[ACGT]AGG CelII 409 GCTCAGC GC^T[ACGT]AGC EaeI 329 CGGCCG [CT]^GGCC[GA] EagI 329 CGGCCG C^GGCCG EclXI 329 CGGCCG C^GGCCG Eco52I 329 CGGCCG C^GGCCG Eco81I 478 CCTAAGG CC^T[ACGT]AGG EcoNI 1063 CCTAATGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FbaI 1797 TGATCA T^GATCA HindIII 1398 AAGCTT A^AGCTT MroI 134 TCCGGA T^CCGGA MslI 1487 CACTGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NcoI 1492 CCATGG C^CATGG NspI 1454 ACATGT [GA]CATG^[CT] PflFI 1320 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 1550 CCATCATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 101 GGGACCC [GA]G^G[AT]CC[CT] RcaI 2115 TCATGA T^CATGA SacI 1706 GAGCTC GAGCT^C SspBI 1483 TGTACA T^GTACA SspI 2308 AATATT AAT^ATT Tth111I 1320 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 1550 CCATCATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### Alw44I 22 GTGCAC G^TGCAC 1777 GTGCAC AlwI 1581 GATCC GGATC(4/5) 2370 GGATC ApaLI 22 GTGCAC G^TGCAC 1777 GTGCAC BanI 153 GGCACC G^G[CT][GA]CC 369 GGTGCC BbsI 733 GAAGAC GAAGAC(2/6) 1693 GAAGAC BcgI 14 CGAGCCCCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 217 GCAGCACCTTCG BglI 526 GCCCGAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 535 GCCGGCGGGGC BpuAI 733 GAAGAC GAAGAC(2/6) 1693 GAAGAC BsmI 1865 GAATGC GAATGC(1/-1) 1952 GCATTC BsrI 1276 CCAGT ACTGG(1/-1) 1899 CCAGT BstF5I 882 CATCC GGATG(2/0) 2343 GGATG CfoI 375 GCGC GCG^C 471 GCGC FokI 882 CATCC GGATG(9/13) 2343 GGATG HhaI 375 GCGC GCG^C 471 GCGC HinP1I 375 GCGC G^CGC 471 GCGC KspI 38 CCGCGG CCGC^GG 157 CCGCGG NaeI 213 GCCGGC GCC^GGC 535 GCCGGC NgoMIV 213 GCCGGC G^CCGGC 535 GCCGGC PleI 1465 GAGTC GAGTC(4/5) 1524 GAGTC PstI 205 CTGCAG CTGCA^G 1184 CTGCAG SacII 38 CCGCGG CCGC^GG 157 CCGCGG SfiI 525 GGCCCGAGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 534 GGCCGGCGGGGCC SmaI 98 CCCGGG CCC^GGG 548 CCCGGG TaqI 1527 TCGA T^CGA 1662 TCGA Tsp45I 1467 GTCAC ^GT[GC]AC 1605 GTCAC TthHB8I 1527 TCGA T^CGA 1662 TCGA XmaCI 98 CCCGGG C^CCGGG 548 CCCGGG XmaI 98 CCCGGG C^CCGGG 548 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 80 enzymes) # ######################################### AccII AciI AcsI AfaI AluI ApoI Asp700I AspHI AvaI AvaII BanII BbvI BcnI BfaI BmyI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrFI BssKI BstNI BstUI Cac8I Cfr10I Cfr13I DdeI DpnII DraI DraII DsaI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI HaeIII HapII HgaI HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TfiI Tru9I TseI Tsp509I TspRI XmnI