Restriction map for
pF1KE4189
(Circular) (Six-base) MAPSORT of: pF1KE4189.seq Check: 9865 from: 1 to: 5138
pF1KE4189 5138 bp
With 182 enzymes: SgfI *
October 24, 2019 20:50 ..
AccI GT'mk_AC
Cuts at: 467 2176 467
Size: 1709 3429
Acc65I G'GTAC_C
Cuts at: 629 2154 629
Size: 1525 3613
AclI AA'CG_TT
Cuts at: 4978 4978
Size: 5138
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 566 679 889 985 999 1458 2963 3395 4142
Size: 113 210 96 14 459 1505 432 747
Cuts at: 4142 4410 566
Size: 268 1294
Fragments arranged by size:
1505 1294 747 459 432 268 210 113
96 14
AflIII A'CryG_T
Cuts at: 506 2490 3594 4571 506
Size: 1984 1104 977 1073
Fragments arranged by size:
1984 1104 1073 977
AgeI A'CCGG_T
Cuts at: 3526 3526
Size: 5138
AleI CACnn'nnGTG
Cuts at: 1378 1378
Size: 5138
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 304 336 304
Size: 32 5106
AlwNI CAG_nnn'CTG
Cuts at: 204 670 867 1146 1185 1356 1468 1588 2443
Size: 466 197 279 39 171 112 120 855
Cuts at: 2443 4010 204
Size: 1567 1332
Fragments arranged by size:
1567 1332 855 466 279 197 171 120
112 39
ApaLI G'TGCA_C
Cuts at: 1371 3908 1371
Size: 2537 2601
ApoI r'AATT_y
Cuts at: 433 910 1108 2142 4491 4747 433
Size: 477 198 1034 2349 256 824
Fragments arranged by size:
2349 1034 824 477 256 198
AseI AT'TA_AT
Cuts at: 20 20
Size: 5138
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5138
AvaI C'yCGr_G
Cuts at: 1045 1691 2158 1045
Size: 646 467 4025
Fragments arranged by size:
4025 646 467
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1789 1822 1789
Size: 33 5105
BamHI G'GATC_C
Cuts at: 2163 2163
Size: 5138
BanI G'GyrC_C
Cuts at: 564 629 2154 2568 2816 2851 564
Size: 65 1525 414 248 35 2851
Fragments arranged by size:
2851 1525 414 248 65 35
BanII G_rGCy'C
Cuts at: 221 1325 1835 1886 2097 2104 2152 3182 221
Size: 1104 510 51 211 7 48 1030 2177
Fragments arranged by size:
2177 1104 1030 510 211 51 48 7
BbeI G_GCGC'C
Cuts at: 2572 2820 2572
Size: 248 4890
BbsI GAAGACnn'nnnn_
Cuts at: 850 908 850
Size: 58 5080
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1036 1070 1286 1320 1036
Size: 34 216 34 4854
Fragments arranged by size:
4854 216 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3036 3797 5138 3036
Size: 761 1341 3036
Fragments arranged by size:
3036 1341 761
BclI T'GATC_A
Cuts at: 1187 2658 1187
Size: 1471 3667
BglII A'GATC_T
Cuts at: 306 2653 4690 306
Size: 2347 2037 754
Fragments arranged by size:
2347 2037 754
BlpI GC'TnA_GC
Cuts at: 856 1319 2250 856
Size: 463 931 3744
Fragments arranged by size:
3744 931 463
Bme1580I G_kGCm'C
Cuts at: 1375 1904 2763 2856 3912 1375
Size: 529 859 93 1056 2601
Fragments arranged by size:
2601 1056 859 529 93
BmgBI CAC'GTC
Cuts at: 723 1458 723
Size: 735 4403
BmrI ACTGGGnnnn_n'
Cuts at: 1507 1676 1814 2098 2350 2431 2766 4910 1507
Size: 169 138 284 252 81 335 2144 1735
Fragments arranged by size:
2144 1735 335 284 252 169 138 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 153 402 3505 153
Size: 249 3103 1786
Fragments arranged by size:
3103 1786 249
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 123 238 271 676 700 1264 1372 2023 2139
Size: 115 33 405 24 564 108 651 116
Cuts at: 2139 123
Size: 3122
Fragments arranged by size:
3122 651 564 405 116 115 108 33
24
Bpu10I CC'TnA_GC
Cuts at: 1612 1612
Size: 5138
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1114 2314 3193 3685 3983 1114
Size: 1200 879 492 298 2269
Fragments arranged by size:
2269 1200 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 240 1076 1528 4842 240
Size: 836 452 3314 536
Fragments arranged by size:
3314 836 536 452
BsaAI yAC'GTr
Cuts at: 509 2491 509
Size: 1982 3156
BsaBI GATnn'nnATC
Cuts at: 6 2652 4689 6
Size: 2646 2037 455
Fragments arranged by size:
2646 2037 455
BsaHI Gr'CG_yC
Cuts at: 2569 2817 3519 5015 2569
Size: 248 702 1496 2692
Fragments arranged by size:
2692 1496 702 248
BsaWI w'CCGG_w
Cuts at: 153 512 1878 2327 2848 3526 3800 3947 4358
Size: 359 1366 449 521 678 274 147 411
Cuts at: 4358 153
Size: 933
Fragments arranged by size:
1366 933 678 521 449 411 359 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 820 850 1559 1589 820
Size: 30 709 30 4369
Fragments arranged by size:
4369 709 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 457 1285 1843 457
Size: 828 558 3752
Fragments arranged by size:
3752 828 558
BseYI C'CCAG_C
Cuts at: 1356 1439 1789 1885 1890 2563 3321 3898 4394
Size: 83 350 96 5 673 758 577 496
Cuts at: 4394 1356
Size: 2100
Fragments arranged by size:
2100 758 673 577 496 350 96 83
5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 427 1081 1170 1570 1621 1844 427
Size: 654 89 400 51 223 3721
Fragments arranged by size:
3721 654 400 223 89 51
BsiEI CG_ry'CG
Cuts at: 104 1746 2680 2726 3934 104
Size: 1642 934 46 1208 1308
Fragments arranged by size:
1642 1308 1208 934 46
BsiHKAI G_wGCw'C
Cuts at: 221 1325 1375 1835 2152 2930 3120 3912 221
Size: 1104 50 460 317 778 190 792 1447
Fragments arranged by size:
1447 1104 792 778 460 317 190 50
Bsp1286I G_dGCh'C
Cuts at: 221 1325 1375 1835 1886 1904 2097 2104 2152
Size: 1104 50 460 51 18 193 7 48
Cuts at: 2152 2763 2856 2930 3120 3182 3912 221
Size: 611 93 74 190 62 730 1447
Fragments arranged by size:
1447 1104 730 611 460 193 190 93
74 62 51 50 48 18 7
BspEI T'CCGG_A
Cuts at: 153 4358 153
Size: 4205 933
BspHI T'CATG_A
Cuts at: 4314 4314
Size: 5138
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1607 2188 2402 2704 3085 1607
Size: 581 214 302 381 3660
Fragments arranged by size:
3660 581 381 302 214
BsrBI CCG'CTC
Cuts at: 50 807 1224 3430 3484 4968 5133 50
Size: 757 417 2206 54 1484 165 55
Fragments arranged by size:
2206 1484 757 417 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3050 3050
Size: 5138
BsrFI r'CCGG_y
Cuts at: 1785 3136 3317 3526 1785
Size: 1351 181 209 3397
Fragments arranged by size:
3397 1351 209 181
BssSI C'ACGA_G
Cuts at: 3409 3767 3409
Size: 358 4780
BstAPI GCAn_nnn'nTGC
Cuts at: 1413 2515 1413
Size: 1102 4036
BstBI TT'CG_AA
Cuts at: 3499 3499
Size: 5138
BstXI CCAn_nnnn'nTGG
Cuts at: 2111 2111
Size: 5138
BstYI r'GATC_y
Cuts at: 1 306 577 2163 2630 2653 2988 3234 4246
Size: 305 271 1586 467 23 335 246 1012
Cuts at: 4246 4332 4344 4690 1
Size: 86 12 346 449
Fragments arranged by size:
1586 1012 467 449 346 335 305 271
246 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 5138
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3088 3269 3432 3088
Size: 181 163 4794
Fragments arranged by size:
4794 181 163
BtsI GCAGTG_nn'
Cuts at: 634 1064 1260 1357 2381 634
Size: 430 196 97 1024 3391
Fragments arranged by size:
3391 1024 430 196 97
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1641 1676 1641
Size: 35 5103
DraI TTT'AAA
Cuts at: 2137 2137
Size: 5138
DrdI GACnn_nn'nnGTC
Cuts at: 232 2844 3702 232
Size: 2612 858 1668
Fragments arranged by size:
2612 1668 858
EaeI y'GGCC_r
Cuts at: 110 1157 1706 1743 1949 2087 2723 2897 3288
Size: 1047 549 37 206 138 636 174 391
Cuts at: 3288 3315 4660 110
Size: 27 1345 588
Fragments arranged by size:
1345 1047 636 588 549 391 206 174
138 37 27
EagI C'GGCC_G
Cuts at: 1743 2723 1743
Size: 980 4158
EarI CTCTTCn'nnn_
Cuts at: 389 920 1226 3161 3371 389
Size: 531 306 1935 210 2156
Fragments arranged by size:
2156 1935 531 306 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3346 3656 3802 4948 3346
Size: 310 146 1146 3536
Fragments arranged by size:
3536 1146 310 146
EcoICRI GAG'CTC
Cuts at: 219 1323 1833 2150 219
Size: 1104 510 317 3207
Fragments arranged by size:
3207 1104 510 317
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 123 238 271 566 676 679 700 889 985
Size: 115 33 295 110 3 21 189 96
Cuts at: 985 999 1264 1372 1458 2023 2139 2963 3395
Size: 14 265 108 86 565 116 824 432
Cuts at: 3395 4142 4410 123
Size: 747 268 851
Fragments arranged by size:
851 824 747 565 432 295 268 265
189 116 115 110 108 96 86 33
21 14 3
EcoO109I rG'GnC_Cy
Cuts at: 170 1051 1395 2277 170
Size: 881 344 882 3031
Fragments arranged by size:
3031 882 881 344
EcoRI G'AATT_C
Cuts at: 433 2142 433
Size: 1709 3429
EcoRV GAT'ATC
Cuts at: 339 339
Size: 5138
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1186 3157 1186
Size: 1971 3167
FspI TGC'GCA
Cuts at: 754 2919 754
Size: 2165 2973
HaeII r_GCGC'y
Cuts at: 2572 2820 3842 4821 2572
Size: 248 1022 979 2889
Fragments arranged by size:
2889 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 118 150 169 201 1656 1674 1688 1706 2980
Size: 32 19 32 1455 18 14 18 1274
Cuts at: 2980 3010 3012 3042 118
Size: 30 2 30 2214
Fragments arranged by size:
2214 1455 1274 32 32 30 30 19
18 18 14 2
HincII GTy'rAC
Cuts at: 2177 2177
Size: 5138
KasI G'GCGC_C
Cuts at: 2568 2816 2568
Size: 248 4890
KpnI G_GTAC'C
Cuts at: 633 2158 633
Size: 1525 3613
MluI A'CGCG_T
Cuts at: 4571 4571
Size: 5138
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 428 3809 3993 428
Size: 3381 184 1573
Fragments arranged by size:
3381 1573 184
MscI TGG'CCA
Cuts at: 112 1159 1708 1951 2089 2899 112
Size: 1047 549 243 138 810 2351
Fragments arranged by size:
2351 1047 810 549 243 138
MslI CAynn'nnrTG
Cuts at: 346 1378 3127 3254 346
Size: 1032 1749 127 2230
Fragments arranged by size:
2230 1749 1032 127
MspA1I CmG'CkG
Cuts at: 534 792 864 1341 1443 1742 1748 1803 2249
Size: 258 72 477 102 299 6 55 446
Cuts at: 2249 2563 2923 3936 4181 534
Size: 314 360 1013 245 1491
Fragments arranged by size:
1491 1013 477 446 360 314 299 258
245 102 72 55 6
NaeI GCC'GGC
Cuts at: 1787 3319 1787
Size: 1532 3606
NarI GG'CG_CC
Cuts at: 2569 2817 2569
Size: 248 4890
NcoI C'CATG_G
Cuts at: 107 107
Size: 5138
NgoMIV G'CCGG_C
Cuts at: 1785 3317 1785
Size: 1532 3606
NotI GC'GGCC_GC
Cuts at: 1743 1743
Size: 5138
NspI r_CATG'y
Cuts at: 252 2191 2691 3598 252
Size: 1939 500 907 1792
Fragments arranged by size:
1939 1792 907 500
PasI CC'CwG_GG
Cuts at: 1627 1627
Size: 5138
PciI A'CATG_T
Cuts at: 3594 3594
Size: 5138
PflMI CCAn_nnn'nTGG
Cuts at: 1704 1704
Size: 5138
PfoI T'CCnGG_A
Cuts at: 212 653 716 800 2000 212
Size: 441 63 84 1200 3350
Fragments arranged by size:
3350 1200 441 84 63
PmeI GTTT'AAAC
Cuts at: 2137 2137
Size: 5138
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4277 4309 4277
Size: 32 5106
PpuMI rG'GwC_Cy
Cuts at: 1395 1395
Size: 5138
PstI C_TGCA'G
Cuts at: 377 533 617 647 1001 1059 1418 1979 2185
Size: 156 84 30 354 58 359 561 206
Cuts at: 2185 2870 377
Size: 685 2645
Fragments arranged by size:
2645 685 561 359 354 206 156 84
58 30
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5138
PvuII CAG'CTG
Cuts at: 792 864 1341 1443 1803 2563 2923 792
Size: 72 477 102 360 760 360 3007
Fragments arranged by size:
3007 760 477 360 360 102 72
RsrII CG'GwC_CG
Cuts at: 3333 3333
Size: 5138
SacI G_AGCT'C
Cuts at: 221 1325 1835 2152 221
Size: 1104 510 317 3207
Fragments arranged by size:
3207 1104 510 317
SalI G'TCGA_C
Cuts at: 2175 2175
Size: 5138
SapI GCTCTTCn'nnn_
Cuts at: 3161 3371 3161
Size: 210 4928
SbfI CC_TGCA'GG
Cuts at: 1979 2185 1979
Size: 206 4932
ScaI AGT'ACT
Cuts at: 4655 4655
Size: 5138
SexAI A'CCwGG_T
Cuts at: 625 1640 1922 625
Size: 1015 282 3841
Fragments arranged by size:
3841 1015 282
SfcI C'TryA_G
Cuts at: 33 373 529 613 643 997 1055 1414 1798
Size: 340 156 84 30 354 58 359 384
Cuts at: 1798 1975 2181 2866 3859 4050 33
Size: 177 206 685 993 191 1121
Fragments arranged by size:
1121 993 685 384 359 354 340 206
191 177 156 84 58 30
SfoI GGC'GCC
Cuts at: 2570 2818 2570
Size: 248 4890
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5138
SmaI CCC'GGG
Cuts at: 2160 2160
Size: 5138
SmlI C'TyrA_G
Cuts at: 1093 2293 3208 3700 3962 1093
Size: 1200 915 492 262 2269
Fragments arranged by size:
2269 1200 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5138
SphI G_CATG'C
Cuts at: 2191 2691 2191
Size: 500 4638
StuI AGG'CCT
Cuts at: 490 587 1099 1130 490
Size: 97 512 31 4498
Fragments arranged by size:
4498 512 97 31
StyI C'CwwG_G
Cuts at: 107 389 779 2272 2626 107
Size: 282 390 1493 354 2619
Fragments arranged by size:
2619 1493 390 354 282
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 930 2721 2854 3313 3522 930
Size: 1791 133 459 209 2546
Fragments arranged by size:
2546 1791 459 209 133
TatI w'GTAC_w
Cuts at: 743 773 4653 743
Size: 30 3880 1228
Fragments arranged by size:
3880 1228 30
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 820 852 1559 1591 4655 4687 820
Size: 32 707 32 3064 32 1271
Fragments arranged by size:
3064 1271 707 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2935 2935
Size: 5138
XbaI T'CTAG_A
Cuts at: 2169 2169
Size: 5138
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2633 2633
Size: 5138
XmaI C'CCGG_G
Cuts at: 2158 2158
Size: 5138
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI ApaI AscI
AvrII BbvCI BfrBI BglI BmtI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstZ17I Bsu36I ClaI DraIII EcoNI
FseI FspAI HindIII HpaI MfeI NdeI NheI NruI
NsiI PacI PmlI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI XmnI ZraI