################################## # NO CUT ENZYMES ( 160 enzymes) # ################################## AatII AccI AccII AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AspHI AspI AviII AvrII BamHI BbeI BbrPI BbvCI BbvI BcgI BciVI BfaI BfrI BglII BlnI BpmI BsaBI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI Bsp1407I BspEI BspHI BspLU11I BspMI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I Cfr10I CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I Fnu4HI FseI FspI HincII HindII HindIII HpaI ItaI KasI KspI MaeI MaeIII MamI MfeI MluI MroI MspA1I MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StyI SwaI TfiI TseI Tsp45I Tsp509I TspRI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### Acc65I 292 GGTACC G^GTACC Asp718I 292 GGTACC G^GTACC AvaI 237 CTCGGG C^[CT]CG[GA]G BalI 2 TGGCCA TGG^CCA BclI 491 TGATCA T^GATCA BglI 37 GCCCTCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaAI 487 TACGTG [CT]AC^GT[GA] BsaI 307 GAGACC GGTCTC(1/5) BsmAI 307 GAGAC GTCTC(1/5) BsmFI 390 GTCCC GGGAC(10/14) BsmI 112 GCATTC GAATGC(1/-1) BsoBI 237 CTCGGG C^[CT]CG[GA]G BspDI 448 ATCGAT AT^CGAT BsrBI 288 GAGCGG CCGCTC(-3/-3) BsrI 435 CCAGT ACTGG(1/-1) BstF5I 59 CATCC GGATG(2/0) BstXI 446 CCATCGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 92 AGATCC [GA]^GATC[CT] CfoI 478 GCGC GCG^C ClaI 448 ATCGAT AT^CGAT DsaI 498 CCACGG C^C[GA][CT]GG EaeI 2 TGGCCA [CT]^GGCC[GA] EarI 415 GAAGAG CTCTTC(1/4) FauI 457 GCGGG CCCGC(4/6) FbaI 491 TGATCA T^GATCA FokI 59 CATCC GGATG(9/13) HaeII 477 GGCGCT [GA]GCGC^[CT] HhaI 478 GCGC GCG^C HinP1I 478 GCGC G^CGC HinfI 87 GACTC G^A[ACGT]TC KpnI 292 GGTACC GGTAC^C Ksp632I 415 GAAGAG CTCTTC(1/4) MflI 92 AGATCC [GA]^GATC[CT] MluNI 2 TGGCCA TGG^CCA MscI 2 TGGCCA TGG^CCA MseI 317 TTAA T^TAA PleI 87 GACTC GAGTC(4/5) PpuMI 158 AGGACCT [GA]G^G[AT]CC[CT] PshAI 192 GACCAACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SfaNI 330 GATGC GCATC(5/9) StuI 233 AGGCCT AGG^CCT TaqI 449 TCGA T^CGA Tru9I 317 TTAA T^TAA TthHB8I 449 TCGA T^CGA XhoII 92 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AcyI 217 GACGCC G[GA]^CG[CT]C 298 GACGCC AfaI 293 GTAC GT^AC 369 GTAC AlwI 93 GATCC GGATC(4/5) 126 GGATC ApaI 44 GGGCCC GGGCC^C 241 GGGCCC BanI 34 GGTGCC G^G[CT][GA]CC 292 GGTACC BanII 44 GGGCCC G[GA]GC[CT]^C 241 GGGCCC BbsI 12 GAAGAC GAAGAC(2/6) 67 GTCTTC BcnI 42 CCGGG CC^[GC]GG 339 CCCGG BlpI 285 GCTGAGC GC^T[ACGT]AGC 480 GCTCAGC Bpu1102I 285 GCTGAGC GC^T[ACGT]AGC 480 GCTCAGC BpuAI 12 GAAGAC GAAGAC(2/6) 67 GTCTTC BsaHI 217 GACGCC G[GA]^CG[CT]C 298 GACGCC BsiYI 270 CCTGTTTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 498 CCACGGGGAGG BslI 270 CCTGTTTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 498 CCACGGGGAGG Cac8I 4 GCCAGC GC[ACGT]^[ACGT]GC 457 GCGGGC CelII 285 GCTGAGC GC^T[ACGT]AGC 480 GCTCAGC DraII 158 AGGACCT [GA]G^G[ACGT]CC[CT] 240 GGGGCCC EcoO109I 158 AGGACCT [GA]G^G[ACGT]CC[CT] 240 GGGGCCC HapII 42 CCGG C^CGG 340 CCGG Hin1I 217 GACGCC G[GA]^CG[CT]C 298 GACGCC HpaII 42 CCGG C^CGG 340 CCGG HphI 254 TCACC GGTGA(8/7) 494 TCACC MaeII 197 ACGT A^CGT 488 ACGT MslI 117 CACACTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 447 CATCGATGTG MspI 42 CCGG C^CGG 340 CCGG MwoI 37 GCCCTCCGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 46 GCCCAGAGAGC NciI 42 CCGGG CC^[GC]GG 339 CCCGG RsaI 293 GTAC GT^AC 369 GTAC ######################################### # 3 OR MORE CUT ENZYMES ( 25 enzymes) # ######################################### AciI AluI AvaII BmyI BsaJI BseRI Bsp1286I BssKI BstNI Cfr13I DdeI DpnII EcoRII HaeIII HgaI MboI MboII MnlI MvaI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI