(Circular) (Six-base) MAPSORT of: pF1KE2633.seq Check: 9946 from: 1 to: 5699 pF1KE2633 5699 bp With 182 enzymes: SgfI * August 3, 2016 15:29 .. AccI GT'mk_AC Cuts at: 1327 1698 2737 1327 Size: 371 1039 4289 Fragments arranged by size: 4289 1039 371 Acc65I G'GTAC_C Cuts at: 2715 2715 Size: 5699 AclI AA'CG_TT Cuts at: 5539 5539 Size: 5699 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 133 298 442 614 743 1010 1075 1163 2233 Size: 165 144 172 129 267 65 88 1070 Cuts at: 2233 2407 2575 3524 3956 4703 4971 133 Size: 174 168 949 432 747 268 861 Fragments arranged by size: 1070 949 861 747 432 268 267 174 172 168 165 144 129 88 65 AflIII A'CryG_T Cuts at: 1295 3051 4155 5132 1295 Size: 1756 1104 977 1862 Fragments arranged by size: 1862 1756 1104 977 AgeI A'CCGG_T Cuts at: 4087 4087 Size: 5699 AlwNI CAG_nnn'CTG Cuts at: 391 721 969 1453 1848 3004 4571 391 Size: 330 248 484 395 1156 1567 1519 Fragments arranged by size: 1567 1519 1156 484 395 330 248 ApaI G_GGCC'C Cuts at: 124 124 Size: 5699 ApaLI G'TGCA_C Cuts at: 197 4469 197 Size: 4272 1427 ApoI r'AATT_y Cuts at: 187 220 241 612 1742 2703 5052 5308 187 Size: 33 21 371 1130 961 2349 256 578 Fragments arranged by size: 2349 1130 961 578 371 256 33 21 AseI AT'TA_AT Cuts at: 20 179 2174 20 Size: 159 1995 3545 Fragments arranged by size: 3545 1995 159 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 5699 AvaI C'yCGr_G Cuts at: 1347 2719 1347 Size: 1372 4327 BamHI G'GATC_C Cuts at: 2609 2724 2609 Size: 115 5584 BanI G'GyrC_C Cuts at: 2715 3129 3377 3412 2715 Size: 414 248 35 5002 Fragments arranged by size: 5002 414 248 35 BanII G_rGCy'C Cuts at: 124 1354 1917 2713 3743 124 Size: 1230 563 796 1030 2080 Fragments arranged by size: 2080 1230 1030 796 563 BbeI G_GCGC'C Cuts at: 3133 3381 3133 Size: 248 5451 BbsI GAAGACnn'nnnn_ Cuts at: 731 1831 1859 2111 2413 731 Size: 1100 28 252 302 4017 Fragments arranged by size: 4017 1100 302 252 28 BciVI GTATCCnnnnn_n' Cuts at: 3597 4358 5699 3597 Size: 761 1341 3597 Fragments arranged by size: 3597 1341 761 BclI T'GATC_A Cuts at: 3219 3219 Size: 5699 BglII A'GATC_T Cuts at: 1892 1928 3214 5251 1892 Size: 36 1286 2037 2340 Fragments arranged by size: 2340 2037 1286 36 BlpI GC'TnA_GC Cuts at: 2811 2811 Size: 5699 Bme1580I G_kGCm'C Cuts at: 124 201 540 3324 3417 4473 124 Size: 77 339 2784 93 1056 1350 Fragments arranged by size: 2784 1350 1056 339 93 77 BmrI ACTGGGnnnn_n' Cuts at: 1179 2911 2992 3327 5471 1179 Size: 1732 81 335 2144 1407 Fragments arranged by size: 2144 1732 1407 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4066 4066 Size: 5699 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 535 763 1286 1471 1563 2047 535 Size: 228 523 185 92 484 4187 Fragments arranged by size: 4187 523 484 228 185 92 Bpu10I CC'TnA_GC Cuts at: 1798 2474 1798 Size: 676 5023 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 335 1151 2434 2875 3754 4246 4544 335 Size: 816 1283 441 879 492 298 1490 Fragments arranged by size: 1490 1283 879 816 492 441 298 BsaI GGTCTCn'nnnn_ Cuts at: 1763 5403 1763 Size: 3640 2059 BsaAI yAC'GTr Cuts at: 196 944 2199 3052 196 Size: 748 1255 853 2843 Fragments arranged by size: 2843 1255 853 748 BsaBI GATnn'nnATC Cuts at: 6 3213 5250 6 Size: 3207 2037 455 Fragments arranged by size: 3207 2037 455 BsaHI Gr'CG_yC Cuts at: 3130 3378 4080 5576 3130 Size: 248 702 1496 3253 Fragments arranged by size: 3253 1496 702 248 BsaWI w'CCGG_w Cuts at: 2888 3409 4087 4361 4508 4919 2888 Size: 521 678 274 147 411 3668 Fragments arranged by size: 3668 678 521 411 274 147 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 283 619 1216 1564 283 Size: 336 597 348 4418 Fragments arranged by size: 4418 597 348 336 BseYI C'CCAG_C Cuts at: 131 335 3124 3882 4459 4955 131 Size: 204 2789 758 577 496 875 Fragments arranged by size: 2789 875 758 577 496 204 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 290 1883 2617 290 Size: 1593 734 3372 Fragments arranged by size: 3372 1593 734 BsiEI CG_ry'CG Cuts at: 104 3241 3287 4495 104 Size: 3137 46 1208 1308 Fragments arranged by size: 3137 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 201 497 1323 2713 3491 3681 4473 201 Size: 296 826 1390 778 190 792 1427 Fragments arranged by size: 1427 1390 826 792 778 296 190 Bsp1286I G_dGCh'C Cuts at: 124 201 497 540 1323 1354 1917 2713 3324 Size: 77 296 43 783 31 563 796 611 Cuts at: 3324 3417 3491 3681 3743 4473 124 Size: 93 74 190 62 730 1350 Fragments arranged by size: 1350 796 783 730 611 563 296 190 93 77 74 62 43 31 BspEI T'CCGG_A Cuts at: 4919 4919 Size: 5699 BspHI T'CATG_A Cuts at: 4875 4875 Size: 5699 BspMI ACCTGCnnnn'nnnn_ Cuts at: 2749 2963 3265 3646 2749 Size: 214 302 381 4802 Fragments arranged by size: 4802 381 302 214 BsrBI CCG'CTC Cuts at: 50 2607 3991 4045 5529 5694 50 Size: 2557 1384 54 1484 165 55 Fragments arranged by size: 2557 1484 1384 165 55 54 BsrDI GCAATG_nn' Cuts at: 1354 3611 1354 Size: 2257 3442 BsrFI r'CCGG_y Cuts at: 3697 3878 4087 3697 Size: 181 209 5309 Fragments arranged by size: 5309 209 181 BssSI C'ACGA_G Cuts at: 3970 4328 3970 Size: 358 5341 BstAPI GCAn_nnn'nTGC Cuts at: 1396 2079 3076 1396 Size: 683 997 4019 Fragments arranged by size: 4019 997 683 BstBI TT'CG_AA Cuts at: 330 4060 330 Size: 3730 1969 BstYI r'GATC_y Cuts at: 1 273 1269 1892 1928 2243 2609 2724 3191 Size: 272 996 623 36 315 366 115 467 Cuts at: 3191 3214 3549 3795 4807 4893 4905 5251 1 Size: 23 335 246 1012 86 12 346 449 Fragments arranged by size: 1012 996 623 467 449 366 346 335 315 272 246 115 86 36 23 12 Bsu36I CC'TnA_GG Cuts at: 508 508 Size: 5699 BtgI C'CryG_G Cuts at: 107 1221 107 Size: 1114 4585 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1555 3649 3830 3993 1555 Size: 2094 181 163 3261 Fragments arranged by size: 3261 2094 181 163 BtsI GCAGTG_nn' Cuts at: 2134 2942 2134 Size: 808 4891 DraI TTT'AAA Cuts at: 1302 2264 2698 1302 Size: 962 434 4303 Fragments arranged by size: 4303 962 434 DrdI GACnn_nn'nnGTC Cuts at: 3405 4263 3405 Size: 858 4841 EaeI y'GGCC_r Cuts at: 3284 3458 3849 3876 5221 3284 Size: 174 391 27 1345 3762 Fragments arranged by size: 3762 1345 391 174 27 EagI C'GGCC_G Cuts at: 3284 3284 Size: 5699 EarI CTCTTCn'nnn_ Cuts at: 152 274 1193 1553 1934 2021 2441 2498 3722 Size: 122 919 360 381 87 420 57 1224 Cuts at: 3722 3932 152 Size: 210 1919 Fragments arranged by size: 1919 1224 919 420 381 360 210 122 87 57 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 3907 4217 4363 5509 3907 Size: 310 146 1146 4097 Fragments arranged by size: 4097 1146 310 146 EcoICRI GAG'CTC Cuts at: 2711 2711 Size: 5699 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 133 298 442 535 614 743 763 1010 1075 Size: 165 144 93 79 129 20 247 65 Cuts at: 1075 1163 1286 1471 1563 2047 2233 2407 2575 Size: 88 123 185 92 484 186 174 168 Cuts at: 2575 3524 3956 4703 4971 133 Size: 949 432 747 268 861 Fragments arranged by size: 949 861 747 484 432 268 247 186 185 174 168 165 144 129 123 93 92 88 79 65 20 EcoO109I rG'GnC_Cy Cuts at: 2838 2838 Size: 5699 EcoRI G'AATT_C Cuts at: 1742 2703 1742 Size: 961 4738 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2290 3718 2290 Size: 1428 4271 FspI TGC'GCA Cuts at: 3480 3480 Size: 5699 HaeII r_GCGC'y Cuts at: 3133 3381 4403 5382 3133 Size: 248 1022 979 3450 Fragments arranged by size: 3450 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1092 1124 1435 1456 1467 1488 1552 1584 3541 Size: 32 311 21 11 21 64 32 1957 Cuts at: 3541 3571 3573 3603 1092 Size: 30 2 30 3188 Fragments arranged by size: 3188 1957 311 64 32 32 30 30 21 21 11 2 HincII GTy'rAC Cuts at: 1511 2738 1511 Size: 1227 4472 HindIII A'AGCT_T Cuts at: 1367 2575 1367 Size: 1208 4491 HpaI GTT'AAC Cuts at: 1511 1511 Size: 5699 KasI G'GCGC_C Cuts at: 3129 3377 3129 Size: 248 5451 KpnI G_GTAC'C Cuts at: 2719 2719 Size: 5699 MluI A'CGCG_T Cuts at: 5132 5132 Size: 5699 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1062 2360 4370 4554 1062 Size: 1298 2010 184 2207 Fragments arranged by size: 2207 2010 1298 184 MscI TGG'CCA Cuts at: 3460 3460 Size: 5699 MslI CAynn'nnrTG Cuts at: 2176 3688 3815 2176 Size: 1512 127 4060 Fragments arranged by size: 4060 1512 127 MspA1I CmG'CkG Cuts at: 339 1791 2810 3124 3484 4497 4742 339 Size: 1452 1019 314 360 1013 245 1296 Fragments arranged by size: 1452 1296 1019 1013 360 314 245 NaeI GCC'GGC Cuts at: 3880 3880 Size: 5699 NarI GG'CG_CC Cuts at: 3130 3378 3130 Size: 248 5451 NcoI C'CATG_G Cuts at: 107 107 Size: 5699 NgoMIV G'CCGG_C Cuts at: 3878 3878 Size: 5699 NspI r_CATG'y Cuts at: 1002 1299 2086 2752 3252 4159 1002 Size: 297 787 666 500 907 2542 Fragments arranged by size: 2542 907 787 666 500 297 PasI CC'CwG_GG Cuts at: 1590 1590 Size: 5699 PciI A'CATG_T Cuts at: 1295 4155 1295 Size: 2860 2839 PflMI CCAn_nnn'nTGG Cuts at: 903 969 1596 903 Size: 66 627 5006 Fragments arranged by size: 5006 627 66 PmeI GTTT'AAAC Cuts at: 2698 2698 Size: 5699 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 484 516 4838 4870 484 Size: 32 4322 32 1313 Fragments arranged by size: 4322 1313 32 32 PspOMI G'GGCC_C Cuts at: 120 120 Size: 5699 PstI C_TGCA'G Cuts at: 2746 3431 2746 Size: 685 5014 PvuI CG_AT'CG Cuts at: 104 104 Size: 5699 PvuII CAG'CTG Cuts at: 339 1791 3124 3484 339 Size: 1452 1333 360 2554 Fragments arranged by size: 2554 1452 1333 360 RsrII CG'GwC_CG Cuts at: 662 3894 662 Size: 3232 2467 SacI G_AGCT'C Cuts at: 2713 2713 Size: 5699 SalI G'TCGA_C Cuts at: 2736 2736 Size: 5699 SapI GCTCTTCn'nnn_ Cuts at: 2021 2441 3722 3932 2021 Size: 420 1281 210 3788 Fragments arranged by size: 3788 1281 420 210 SbfI CC_TGCA'GG Cuts at: 2746 2746 Size: 5699 ScaI AGT'ACT Cuts at: 5216 5216 Size: 5699 SexAI A'CCwGG_T Cuts at: 1772 1772 Size: 5699 SfcI C'TryA_G Cuts at: 33 413 850 1328 1786 2126 2742 3427 4420 Size: 380 437 478 458 340 616 685 993 Cuts at: 4420 4611 33 Size: 191 1121 Fragments arranged by size: 1121 993 685 616 478 458 437 380 340 191 SfoI GGC'GCC Cuts at: 3131 3379 3131 Size: 248 5451 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 5699 SmaI CCC'GGG Cuts at: 2721 2721 Size: 5699 SmlI C'TyrA_G Cuts at: 350 1166 2413 2854 3769 4261 4523 350 Size: 816 1247 441 915 492 262 1526 Fragments arranged by size: 1526 1247 915 816 492 441 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 5699 SphI G_CATG'C Cuts at: 2752 3252 2752 Size: 500 5199 SspI AAT'ATT Cuts at: 369 369 Size: 5699 StuI AGG'CCT Cuts at: 748 1921 748 Size: 1173 4526 StyI C'CwwG_G Cuts at: 107 1476 2627 2833 3187 107 Size: 1369 1151 206 354 2619 Fragments arranged by size: 2619 1369 1151 354 206 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 650 3282 3415 3874 4083 650 Size: 2632 133 459 209 2266 Fragments arranged by size: 2632 2266 459 209 133 TatI w'GTAC_w Cuts at: 5214 5214 Size: 5699 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5216 5248 5216 Size: 32 5667 Tth111I GACn'n_nGTC Cuts at: 1566 3496 1566 Size: 1930 3769 XbaI T'CTAG_A Cuts at: 807 2730 807 Size: 1923 3776 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1495 3194 1495 Size: 1699 4000 XmaI C'CCGG_G Cuts at: 2719 2719 Size: 5699 XmnI GAAnn'nnTTC Cuts at: 479 479 Size: 5699 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI PmeI PpiI PspOMI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AleI AlfI AloI AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI BsaXI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI MfeI NdeI NheI NotI NruI NsiI PacI PfoI PmlI PpuMI PshAI PsiI PspXI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI SwaI XhoI ZraI