Restriction map for
pF1KE1597
(Linear) (Six-base) MAPSORT of: KIEE1597.seq Check: 2063 from: 1 to: 9240
KIEE1597.orf
With 182 enzymes: SgfI *
September 9, 2010 13:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1374 1560 4728 9240
Size: 1374 186 3168 4512
Fragments arranged by size:
4512 3168 1374 186
AatII G_ACGT'C
Cuts at: 0 635 9240
Size: 635 8605
AccI GT'mk_AC
Cuts at: 0 1237 1633 2299 3496 4069 8197 9240
Size: 1237 396 666 1197 573 4128 1043
Fragments arranged by size:
4128 1237 1197 1043 666 573 396
Acc65I G'GTAC_C
Cuts at: 0 2137 3189 9240
Size: 2137 1052 6051
Fragments arranged by size:
6051 2137 1052
AclI AA'CG_TT
Cuts at: 0 7556 9240
Size: 7556 1684
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1025 1148 1751 2810 3248 3659 3929 4331
Size: 1025 123 603 1059 438 411 270 402
Cuts at: 4331 4763 5549 5684 5972 6360 9167 9240
Size: 432 786 135 288 388 2807 73
Fragments arranged by size:
2807 1059 1025 786 603 438 432 411
402 388 288 270 135 123 73
AfeI AGC'GCT
Cuts at: 0 8231 9240
Size: 8231 1009
AflIII A'CryG_T
Cuts at: 0 1738 7122 7718 8498 9240
Size: 1738 5384 596 780 742
Fragments arranged by size:
5384 1738 780 742 596
AleI CACnn'nnGTG
Cuts at: 0 2026 3355 3478 3724 4204 4834 6595 9240
Size: 2026 1329 123 246 480 630 1761 2645
Fragments arranged by size:
2645 2026 1761 1329 630 480 246 123
AlwNI CAG_nnn'CTG
Cuts at: 0 1783 1989 2575 2641 5995 6619 7771 9240
Size: 1783 206 586 66 3354 624 1152 1469
Fragments arranged by size:
3354 1783 1469 1152 624 586 206 66
ApaLI G'TGCA_C
Cuts at: 0 8349 9240
Size: 8349 891
ApoI r'AATT_y
Cuts at: 0 1143 1590 2040 2257 2391 2843 3843 3917
Size: 1143 447 450 217 134 452 1000 74
Cuts at: 3917 3978 5584 5831 6473 6805 6816 6825 6847
Size: 61 1606 247 642 332 11 9 22
Cuts at: 6847 7428 7613 7847 8097 8333 8554 8760 9240
Size: 581 185 234 250 236 221 206 480
Fragments arranged by size:
1606 1143 1000 642 581 480 452 450
447 332 250 247 236 234 221 217
206 185 134 74 61 22 11 9
AseI AT'TA_AT
Cuts at: 0 5696 9240
Size: 5696 3544
AvaI C'yCGr_G
Cuts at: 0 3863 9240
Size: 3863 5377
AvrII C'CTAG_G
Cuts at: 0 477 2758 7782 9240
Size: 477 2281 5024 1458
Fragments arranged by size:
5024 2281 1458 477
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2165 2198 2459 2492 5186 5219 9240
Size: 2165 33 261 33 2694 33 4021
Fragments arranged by size:
4021 2694 2165 261 33 33 33
BamHI G'GATC_C
Cuts at: 0 6779 9240
Size: 6779 2461
BanI G'GyrC_C
Cuts at: 0 1896 2137 3189 3607 4905 5017 5980 6427
Size: 1896 241 1052 418 1298 112 963 447
Cuts at: 6427 9240
Size: 2813
Fragments arranged by size:
2813 1896 1298 1052 963 447 418 241
112
BanII G_rGCy'C
Cuts at: 0 8641 9240
Size: 8641 599
BbsI GAAGACnn'nnnn_
Cuts at: 0 263 635 1201 3298 4769 6617 8514 8791
Size: 263 372 566 2097 1471 1848 1897 277
Cuts at: 8791 9240
Size: 449
Fragments arranged by size:
2097 1897 1848 1471 566 449 372 277
263
BbvCI CC'TCA_GC
Cuts at: 0 2576 3012 3077 7403 9240
Size: 2576 436 65 4326 1837
Fragments arranged by size:
4326 2576 1837 436 65
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 6683 6717 9240
Size: 6683 34 2523
Fragments arranged by size:
6683 2523 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 416 2193 4227 9240
Size: 416 1777 2034 5013
Fragments arranged by size:
5013 2034 1777 416
BclI T'GATC_A
Cuts at: 0 8339 8384 9240
Size: 8339 45 856
Fragments arranged by size:
8339 856 45
BfrBI ATG'CAT
Cuts at: 0 173 4629 7241 9240
Size: 173 4456 2612 1999
Fragments arranged by size:
4456 2612 1999 173
BglI GCCn_nnn'nGGC
Cuts at: 0 1993 3598 7690 9240
Size: 1993 1605 4092 1550
Fragments arranged by size:
4092 1993 1605 1550
BglII A'GATC_T
Cuts at: 0 5537 8619 9240
Size: 5537 3082 621
Fragments arranged by size:
5537 3082 621
Bme1580I G_kGCm'C
Cuts at: 0 485 1281 4155 8353 9240
Size: 485 796 2874 4198 887
Fragments arranged by size:
4198 2874 887 796 485
BmrI ACTGGGnnnn_n'
Cuts at: 0 1283 1706 6238 6602 7990 9240
Size: 1283 423 4532 364 1388 1250
Fragments arranged by size:
4532 1388 1283 1250 423 364
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1674 4704 6339 7868 9240
Size: 1674 3030 1635 1529 1372
Fragments arranged by size:
3030 1674 1635 1529 1372
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 788 1418 1932 2108 5075 6309 6587 8506
Size: 788 630 514 176 2967 1234 278 1919
Cuts at: 8506 9240
Size: 734
Fragments arranged by size:
2967 1919 1234 788 734 630 514 278
176
Bpu10I CC'TnA_GC
Cuts at: 0 1406 2576 3012 3077 3365 6512 7017 7403
Size: 1406 1170 436 65 288 3147 505 386
Cuts at: 7403 9240
Size: 1837
Fragments arranged by size:
3147 1837 1406 1170 505 436 386 288
65
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 186 475 3509 4017 9109 9240
Size: 186 289 3034 508 5092 131
Fragments arranged by size:
5092 3034 508 289 186 131
BsaI GGTCTCn'nnnn_
Cuts at: 0 275 1537 1645 1951 2413 3367 3610 3628
Size: 275 1262 108 306 462 954 243 18
Cuts at: 3628 3901 9240
Size: 273 5339
Fragments arranged by size:
5339 1262 954 462 306 275 273 243
108 18
BsaAI yAC'GTr
Cuts at: 0 1741 9240
Size: 1741 7499
BsaHI Gr'CG_yC
Cuts at: 0 632 9115 9240
Size: 632 8483 125
Fragments arranged by size:
8483 632 125
BsaWI w'CCGG_w
Cuts at: 0 8245 9240
Size: 8245 995
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 855 885 1017 1047 1416 1446 4506 4536
Size: 855 30 132 30 369 30 3060 30
Cuts at: 4536 5544 5574 8267 8297 9240
Size: 1008 30 2693 30 943
Fragments arranged by size:
3060 2693 1008 943 855 369 132 30
30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1647 3094 3547 5549 5552 8250 9240
Size: 1647 1447 453 2002 3 2698 990
Fragments arranged by size:
2698 2002 1647 1447 990 453 3
BseYI C'CCAG_C
Cuts at: 0 4846 4993 7446 8085 9240
Size: 4846 147 2453 639 1155
Fragments arranged by size:
4846 2453 1155 639 147
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 773 878 8724 9152 9240
Size: 773 105 7846 428 88
Fragments arranged by size:
7846 773 428 105 88
BsiHKAI G_wGCw'C
Cuts at: 0 1418 2875 3143 5053 6196 8353 9104 9240
Size: 1418 1457 268 1910 1143 2157 751 136
Fragments arranged by size:
2157 1910 1457 1418 1143 751 268 136
BsmI GAATG_Cn'
Cuts at: 0 2381 4317 7404 7742 9240
Size: 2381 1936 3087 338 1498
Fragments arranged by size:
3087 2381 1936 1498 338
Bsp1286I G_dGCh'C
Cuts at: 0 485 1281 1418 2875 3143 4155 5053 6196
Size: 485 796 137 1457 268 1012 898 1143
Cuts at: 6196 8353 8641 9104 9240
Size: 2157 288 463 136
Fragments arranged by size:
2157 1457 1143 1012 898 796 485 463
288 268 137 136
BspHI T'CATG_A
Cuts at: 0 2058 8720 9240
Size: 2058 6662 520
Fragments arranged by size:
6662 2058 520
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1374 1560 2316 4728 9240
Size: 1374 186 756 2412 4512
Fragments arranged by size:
4512 2412 1374 756 186
BsrDI GCAATG_nn'
Cuts at: 0 1959 3046 4543 5813 7614 7794 9240
Size: 1959 1087 1497 1270 1801 180 1446
Fragments arranged by size:
1959 1801 1497 1446 1270 1087 180
BsrFI r'CCGG_y
Cuts at: 0 9047 9240
Size: 9047 193
BsrGI T'GTAC_A
Cuts at: 0 1710 3714 4654 6296 9240
Size: 1710 2004 940 1642 2944
Fragments arranged by size:
2944 2004 1710 1642 940
BssSI C'ACGA_G
Cuts at: 0 4146 9240
Size: 4146 5094
BstAPI GCAn_nnn'nTGC
Cuts at: 0 8872 9240
Size: 8872 368
BstBI TT'CG_AA
Cuts at: 0 8313 9240
Size: 8313 927
BstEII G'GTnAC_C
Cuts at: 0 3090 9240
Size: 3090 6150
BstXI CCAn_nnnn'nTGG
Cuts at: 0 766 3186 3738 3792 4179 4989 5718 8038
Size: 766 2420 552 54 387 810 729 2320
Cuts at: 8038 8271 9240
Size: 233 969
Fragments arranged by size:
2420 2320 969 810 766 729 552 387
233 54
BstYI r'GATC_y
Cuts at: 0 2854 5537 5992 6779 7368 8619 9219 9240
Size: 2854 2683 455 787 589 1251 600 21
Fragments arranged by size:
2854 2683 1251 787 600 589 455 21
Bsu36I CC'TnA_GG
Cuts at: 0 4337 6314 9240
Size: 4337 1977 2926
Fragments arranged by size:
4337 2926 1977
BtgI C'CryG_G
Cuts at: 0 1076 2200 3240 3272 3299 5464 5844 9240
Size: 1076 1124 1040 32 27 2165 380 3396
Fragments arranged by size:
3396 2165 1124 1076 1040 380 32 27
BtsI GCAGTG_nn'
Cuts at: 0 673 947 2141 2576 5021 7136 8288 8444
Size: 673 274 1194 435 2445 2115 1152 156
Cuts at: 8444 8992 9240
Size: 548 248
Fragments arranged by size:
2445 2115 1194 1152 673 548 435 274
248 156
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 3204 3239 4734 4769 7926 7961 9240
Size: 3204 35 1495 35 3157 35 1279
Fragments arranged by size:
3204 3157 1495 1279 35 35 35
DraI TTT'AAA
Cuts at: 0 2262 7427 9240
Size: 2262 5165 1813
Fragments arranged by size:
5165 2262 1813
DraIII CAC_nnn'GTG
Cuts at: 0 6232 7507 8961 9240
Size: 6232 1275 1454 279
Fragments arranged by size:
6232 1454 1275 279
DrdI GACnn_nn'nnGTC
Cuts at: 0 2961 3828 9240
Size: 2961 867 5412
Fragments arranged by size:
5412 2961 867
EaeI y'GGCC_r
Cuts at: 0 1299 3116 4227 7682 7890 9240
Size: 1299 1817 1111 3455 208 1350
Fragments arranged by size:
3455 1817 1350 1299 1111 208
EarI CTCTTCn'nnn_
Cuts at: 0 105 409 578 4737 6764 6896 7017 7457
Size: 105 304 169 4159 2027 132 121 440
Cuts at: 7457 9036 9240
Size: 1579 204
Fragments arranged by size:
4159 2027 1579 440 304 204 169 132
121 105
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 7572 8808 9240
Size: 7572 1236 432
Fragments arranged by size:
7572 1236 432
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 788 1025 1148 1418 1751 1932 2108 2810
Size: 788 237 123 270 333 181 176 702
Cuts at: 2810 3248 3659 3929 4331 4763 5075 5549 5684
Size: 438 411 270 402 432 312 474 135
Cuts at: 5684 5972 6309 6360 6587 8506 9167 9240
Size: 288 337 51 227 1919 661 73
Fragments arranged by size:
1919 788 702 661 474 438 432 411
402 337 333 312 288 270 270 237
227 181 176 135 123 73 51
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1904 5825 7799 9240
Size: 1904 3921 1974 1441
Fragments arranged by size:
3921 1974 1904 1441
EcoO109I rG'GnC_Cy
Cuts at: 0 1909 1986 5171 7190 8881 9240
Size: 1909 77 3185 2019 1691 359
Fragments arranged by size:
3185 2019 1909 1691 359 77
EcoRI G'AATT_C
Cuts at: 0 1143 3843 5831 6473 6825 7847 8097 8760
Size: 1143 2700 1988 642 352 1022 250 663
Cuts at: 8760 9240
Size: 480
Fragments arranged by size:
2700 1988 1143 1022 663 642 480 352
250
EcoRV GAT'ATC
Cuts at: 0 2918 4277 6143 9240
Size: 2918 1359 1866 3097
Fragments arranged by size:
3097 2918 1866 1359
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 5433 9111 9240
Size: 5433 3678 129
Fragments arranged by size:
5433 3678 129
HaeII r_GCGC'y
Cuts at: 0 8233 9240
Size: 8233 1007
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 26 58 184 216 937 969 1213 1245
Size: 26 32 126 32 721 32 244 32
Cuts at: 1245 1615 1647 2350 2382 4266 4298 4381 4413
Size: 370 32 703 32 1884 32 83 32
Cuts at: 4413 4989 5021 5529 5544 5561 5576 6127 6159
Size: 576 32 508 15 17 15 551 32
Cuts at: 6159 6345 6377 7353 7385 7656 7688 8240 8272
Size: 186 32 976 32 271 32 552 32
Cuts at: 8272 8411 8443 9105 9137 9240
Size: 139 32 662 32 103
Fragments arranged by size:
1884 976 721 703 662 576 552 551
508 370 271 244 186 139 126 103
83 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 26 17 15 15
HincII GTy'rAC
Cuts at: 0 645 666 1226 3668 5759 9113 9131 9240
Size: 645 21 560 2442 2091 3354 18 109
Fragments arranged by size:
3354 2442 2091 645 560 109 21 18
HindIII A'AGCT_T
Cuts at: 0 25 9192 9240
Size: 25 9167 48
Fragments arranged by size:
9167 48 25
KpnI G_GTAC'C
Cuts at: 0 2141 3193 9240
Size: 2141 1052 6047
Fragments arranged by size:
6047 2141 1052
MfeI C'AATT_G
Cuts at: 0 2447 5364 7096 9240
Size: 2447 2917 1732 2144
Fragments arranged by size:
2917 2447 2144 1732
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 3791 4469 4966 6502 8638 9045 9240
Size: 3791 678 497 1536 2136 407 195
Fragments arranged by size:
3791 2136 1536 678 497 407 195
MscI TGG'CCA
Cuts at: 0 1301 3118 4229 7892 9240
Size: 1301 1817 1111 3663 1348
Fragments arranged by size:
3663 1817 1348 1301 1111
MslI CAynn'nnrTG
Cuts at: 0 351 2026 2199 2807 3355 3478 3724 4204
Size: 351 1675 173 608 548 123 246 480
Cuts at: 4204 4834 4987 5806 6462 6595 7751 8689 8947
Size: 630 153 819 656 133 1156 938 258
Cuts at: 8947 9240
Size: 293
Fragments arranged by size:
1675 1156 938 819 656 630 608 548
480 351 293 258 246 173 153 133
123
MspA1I CmG'CkG
Cuts at: 0 1297 1324 1429 1567 1843 2179 2851 3001
Size: 1297 27 105 138 276 336 672 150
Cuts at: 3001 4411 5662 8434 9240
Size: 1410 1251 2772 806
Fragments arranged by size:
2772 1410 1297 1251 806 672 336 276
150 138 105 27
NcoI C'CATG_G
Cuts at: 0 1076 2200 3240 3299 5464 5844 9240
Size: 1076 1124 1040 59 2165 380 3396
Fragments arranged by size:
3396 2165 1124 1076 1040 380 59
NdeI CA'TA_TG
Cuts at: 0 123 3038 4224 9240
Size: 123 2915 1186 5016
Fragments arranged by size:
5016 2915 1186 123
NsiI A_TGCA'T
Cuts at: 0 175 4631 7243 9240
Size: 175 4456 2612 1997
Fragments arranged by size:
4456 2612 1997 175
NspI r_CATG'y
Cuts at: 0 173 8502 9240
Size: 173 8329 738
Fragments arranged by size:
8329 738 173
PasI CC'CwG_GG
Cuts at: 0 2895 3792 7452 9240
Size: 2895 897 3660 1788
Fragments arranged by size:
3660 2895 1788 897
PciI A'CATG_T
Cuts at: 0 8498 9240
Size: 8498 742
PflMI CCAn_nnn'nTGG
Cuts at: 0 573 3058 9240
Size: 573 2485 6182
Fragments arranged by size:
6182 2485 573
PfoI T'CCnGG_A
Cuts at: 0 572 4912 5644 6564 9240
Size: 572 4340 732 920 2676
Fragments arranged by size:
4340 2676 920 732 572
PpuMI rG'GwC_Cy
Cuts at: 0 1909 5171 7190 8881 9240
Size: 1909 3262 2019 1691 359
Fragments arranged by size:
3262 2019 1909 1691 359
PsiI TTA'TAA
Cuts at: 0 6799 8236 9240
Size: 6799 1437 1004
Fragments arranged by size:
6799 1437 1004
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 4958 4990 6201 6233 9240
Size: 4958 32 1211 32 3007
Fragments arranged by size:
4958 3007 1211 32 32
PstI C_TGCA'G
Cuts at: 0 8015 8439 9240
Size: 8015 424 801
Fragments arranged by size:
8015 801 424
PvuII CAG'CTG
Cuts at: 0 1297 1324 1429 1567 1843 2179 2851 3001
Size: 1297 27 105 138 276 336 672 150
Cuts at: 3001 4411 5662 8434 9240
Size: 1410 1251 2772 806
Fragments arranged by size:
2772 1410 1297 1251 806 672 336 276
150 138 105 27
SanDI GG'GwC_CC
Cuts at: 0 8881 9240
Size: 8881 359
SapI GCTCTTCn'nnn_
Cuts at: 0 6764 7017 9240
Size: 6764 253 2223
Fragments arranged by size:
6764 2223 253
ScaI AGT'ACT
Cuts at: 0 5526 9240
Size: 5526 3714
SexAI A'CCwGG_T
Cuts at: 0 8372 9240
Size: 8372 868
SfcI C'TryA_G
Cuts at: 0 1319 1775 1838 2159 2561 2945 2996 4457
Size: 1319 456 63 321 402 384 51 1461
Cuts at: 4457 4889 6098 8011 8198 8435 9240
Size: 432 1209 1913 187 237 805
Fragments arranged by size:
1913 1461 1319 1209 805 456 432 402
384 321 237 187 63 51
SmaI CCC'GGG
Cuts at: 0 3865 9240
Size: 3865 5375
SmlI C'TyrA_G
Cuts at: 0 201 490 3524 4032 9124 9240
Size: 201 289 3034 508 5092 116
Fragments arranged by size:
5092 3034 508 289 201 116
SphI G_CATG'C
Cuts at: 0 173 9240
Size: 173 9067
SspI AAT'ATT
Cuts at: 0 2083 5292 7380 9240
Size: 2083 3209 2088 1860
Fragments arranged by size:
3209 2088 2083 1860
StyI C'CwwG_G
Cuts at: 0 267 477 1076 1339 1558 1892 2200 2332
Size: 267 210 599 263 219 334 308 132
Cuts at: 2332 2758 3240 3299 5464 5844 6775 7782 9240
Size: 426 482 59 2165 380 931 1007 1458
Fragments arranged by size:
2165 1458 1007 931 599 482 426 380
334 308 267 263 219 210 132 59
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1840 2887 6027 6167 6220 8077 8179 8999
Size: 1840 1047 3140 140 53 1857 102 820
Cuts at: 8999 9240
Size: 241
Fragments arranged by size:
3140 1857 1840 1047 820 241 140 102
53
TatI w'GTAC_w
Cuts at: 0 545 718 1710 3714 4654 5524 6296 9240
Size: 545 173 992 2004 940 870 772 2944
Fragments arranged by size:
2944 2004 992 940 870 772 545 173
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1416 1448 1692 1724 4367 4399 8267 8299
Size: 1416 32 244 32 2643 32 3868 32
Cuts at: 8299 9240
Size: 941
Fragments arranged by size:
3868 2643 1416 941 244 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 6357 6390 9240
Size: 6357 33 2850
Fragments arranged by size:
6357 2850 33
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 4986 5574 7723 8199 9240
Size: 4986 588 2149 476 1041
Fragments arranged by size:
4986 2149 1041 588 476
XmaI C'CCGG_G
Cuts at: 0 3863 9240
Size: 3863 5377
XmnI GAAnn'nnTTC
Cuts at: 0 1091 8896 9240
Size: 1091 7805 344
Fragments arranged by size:
7805 1091 344
ZraI GAC'GTC
Cuts at: 0 633 9240
Size: 633 8607
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AleI AlwNI ApaLI ApoI AseI AvaI AvrII BaeI
BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI
BfrBI BglI BglII Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtsI CspCI DraI DraIII DrdI EaeI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI HaeII
Hin4I HincII HindIII KpnI MfeI MmeI MscI MslI
MspA1I NcoI NdeI NsiI NspI PasI PciI PflMI
PfoI PpuMI PsiI PsrI PstI PvuII SanDI SapI
ScaI SexAI SfcI SmaI SmlI SphI SspI StyI
TaqII TatI TstI Tth111I XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AflII AgeI AhdI AlfI AloI ApaI AscI AsiSI
BbeI BlpI BmgBI BmtI BsaBI BsiEI BsiWI BsmBI
BspEI BsrBI BssHII BstZ17I BtgZI ClaI EagI EcoICRI
FseI FspI FspAI HpaI KasI MluI NaeI NarI
NgoMIV NheI NotI NruI PacI PmeI PmlI PpiI
PshAI PspOMI PspXI PvuI RsrII SacI SacII SalI
SbfI SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI
StuI SwaI XbaI XhoI