Restriction map for pF1KE3378
##################################
# NO CUT ENZYMES (  105 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AflII       AgeI        AhdI        Alw44I      ApaI        
ApaLI       AscI        Asp718I     AspEI       AspHI       
AspI        AviII       AvrII       BalI        BanII       
BbeI        BbrPI       BbvCI       BcnI        BfrI        
BglI        BglII       BlnI        BmyI        BsaBI       
BsaHI       BsaWI       BseAI       BsiEI       BsiHKAI     
BsiWI       BsmBI       Bsp1286I    Bsp1407I    BspDI       
BspEI       BspMI       BsrDI       BsrGI       BssHII      
BssSI       Bst1107I    BstXI       BstZ17I     ClaI        
CpoI        DraIII      DrdI        EaeI        EagI        
Eam1105I    EclXI       Eco52I      FseI        FspI        
Hin1I       KasI        KpnI        KspI        MamI        
MluI        MluNI       MroI        MscI        NarI        
NciI        NcoI        NdeI        NheI        NotI        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        Psp1406I    PvuI        
RsrII       SacI        SacII       SalI        SbfI        
SexAI       SfiI        SfoI        SgrAI       SmaI        
SpeI        SphI        Sse8387I    SspBI       Tth111I     
Van91I      XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   61 enzymes) #
###################################

AccI         1970  GTCTAC               GT^[AC][GT]AC
AccII        3373  CGCG                 CG^CG
AflIII       1935  ACATGT               A^C[GA][CT]GT
Aor51HI       824  AGCGCT               AGC^GCT
AvaI         1332  CCCGAG               C^[CT]CG[GA]G
BamHI        2640  GGATCC               G^GATCC
BanI         1230  GGTGCC               G^G[CT][GA]CC
BbsI          635  GAAGAC               GAAGAC(2/6)
BcgI          234  GCAGGAAATTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI         3342  TGATCA               T^GATCA
BlpI          148  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      148  GCTGAGC              GC^T[ACGT]AGC
BpuAI         635  GAAGAC               GAAGAC(2/6)
BsaAI         847  TACGTA               [CT]AC^GT[GA]
BsaI         1029  GAGACC               GGTCTC(1/5)
BsoBI        1332  CCCGAG               C^[CT]CG[GA]G
BspHI        1067  TCATGA               T^CATGA
BspLU11I     1935  ACATGT               A^CATGT
BsrBI        2683  GAGCGG               CCGCTC(-3/-3)
BsrFI          80  GCCGGC               [GA]^CCGG[CT]
BstAPI        457  GCAGCAGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII       2593  GGTGACC              G^GT[ACGT]ACC
BstPI        2593  GGTGACC              G^GT[ACGT]ACC
BstUI        3373  CGCG                 CG^CG
CelII         148  GCTGAGC              GC^T[ACGT]AGC
CfoI          825  GCGC                 GCG^C
Cfr10I         80  GCCGGC               [GA]^CCGG[CT]
DraII        2970  AGGACCC              [GA]G^G[ACGT]CC[CT]
DsaI         2602  CCACGG               C^C[GA][CT]GG
Eco47III      824  AGCGCT               AGC^GCT
EcoO109I     2970  AGGACCC              [GA]G^G[ACGT]CC[CT]
EcoO65I      2593  GGTGACC              G^GT[ACGT]ACC
EcoRI         406  GAATTC               G^AATTC
EcoRV        2263  GATATC               GAT^ATC
EcoT14I      1763  CCAAGG               C^C[AT][AT]GG
EcoT22I      3218  ATGCAT               ATGCA^T
FbaI         3342  TGATCA               T^GATCA
HaeII         824  AGCGCT               [GA]GCGC^[CT]
HapII          81  CCGG                 C^CGG
HhaI          825  GCGC                 GCG^C
HinP1I        825  GCGC                 G^CGC
HpaI          514  GTTAAC               GTT^AAC
HpaII          81  CCGG                 C^CGG
MaeII         848  ACGT                 A^CGT
MspA1I        461  CAGCTG               C[AC]G^C[GT]G
MspI           81  CCGG                 C^CGG
MvnI         3373  CGCG                 CG^CG
NaeI           80  GCCGGC               GCC^GGC
NgoMIV         80  GCCGGC               G^CCGGC
NruI         3372  TCGCGA               TCG^CGA
NsiI         3218  ATGCAT               ATGCA^T
PpuMI        2970  AGGACCC              [GA]G^G[AT]CC[CT]
PstI          455  CTGCAG               CTGCA^G
PvuII         461  CAGCTG               CAG^CTG
RcaI         1067  TCATGA               T^CATGA
SnaBI         847  TACGTA               TAC^GTA
SspI         2497  AATATT               AAT^ATT
StuI           40  AGGCCT               AGG^CCT
StyI         1763  CCAAGG               C^C[AT][AT]GG
SwaI         1914  ATTTAAAT             ATTT^AAAT
XcmI          874  CCACCTGGGGCATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AlwNI         458  CAGCAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1454  CAGTCACTG           
AocI          111  CCTTAGG              CC^T[ACGT]AGG
             1597  CCTGAGG             
Asp700I      1604  GAACCGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2449  GAAAGTGTTC          
AvaII        1660  GGACC                G^G[AT]CC
             2971  GGACC               
BciVI        1556  GGATAC               GTATCC(6/5)
             2776  GGATAC              
BpmI         1316  CTCCAG               CTGGAG(16/14)
             3045  CTCCAG              
BsmI         1037  GAATGC               GAATGC(1/-1)
             3220  GCATTC              
Bsu36I        111  CCTTAGG              CC^T[ACGT]AGG
             1597  CCTGAGG             
DraI         1915  TTTAAA               TTT^AAA
             3028  TTTAAA              
Eco81I        111  CCTTAGG              CC^T[ACGT]AGG
             1597  CCTGAGG             
FauI          573  CCCGC                CCCGC(4/6)
             2007  GCGGG               
HgaI          216  GACGC                GACGC(5/10)
             1968  GCGTC               
HincII        171  GTCAAC               GT[CT]^[GA]AC
              514  GTTAAC              
HindII        171  GTCAAC               GT[CT]^[GA]AC
              514  GTTAAC              
HindIII      1755  AAGCTT               A^AGCTT
             2939  AAGCTT              
MfeI          401  CAATTG               C^AATTG
             3035  CAATTG              
MunI          401  CAATTG               C^AATTG
             3035  CAATTG              
NspI         1935  ACATGT               [GA]CATG^[CT]
             3216  ACATGC              
PshAI         638  GACCTTTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             3059  GACAGCAGTC          
SapI         1870  GAAGAGC              GCTCTTC(1/4)
              364  GCTCTTC             
ScaI          289  AGTACT               AGT^ACT
             2579  AGTACT              
SfcI          455  CTGCAG               C^T[GA][CT]AG
             1522  CTACAG              
XmnI         1604  GAACCGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2449  GAAAGTGTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   69 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        AseI        AsnI        BbvI        BfaI        
BsaJI       BseRI       BsgI        BsiYI       BslI        
BsmAI       BsmFI       BsrI        BssKI       BstBI       
BstF5I      BstNI       BstYI       Cac8I       Cfr13I      
DdeI        DpnII       EarI        EcoNI       EcoRII      
Fnu4HI      FokI        HaeIII      HinfI       HphI        
ItaI        Ksp632I     MaeI        MaeIII      MboI        
MboII       MflI        MnlI        MseI        MslI        
MvaI        MwoI        NdeII       NlaIII      NlaIV       
NspV        PleI        PshBI       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfuI        SmlI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XhoII       
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