Restriction map for pF1KB9166
##################################
# NO CUT ENZYMES (   75 enzymes) #
##################################

AccIII      AclI        AcsI        AflII       AgeI        
Aor51HI     ApoI        AscI        AseI        AsnI        
Asp700I     AviII       BbeI        BciVI       BfrI        
BglII       BlpI        Bpu1102I    BsaBI       BseAI       
BsiWI       BsmI        Bsp1407I    BspEI       BspHI       
BsrGI       Bst1107I    BstEII      BstPI       BstZ17I     
CelII       CpoI        DraI        Eco47III    EcoO65I     
EcoRI       EcoT22I     FseI        FspI        HindIII     
HpaI        KasI        KspI        MamI        MfeI        
MluI        MroI        MunI        NarI        NdeI        
NotI        NruI        NsiI        PacI        PaeR7I      
PinAI       PmeI        PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacII       SbfI        SfiI        
SfoI        SnaBI       SpeI        Sse8387I    SspBI       
SspI        SwaI        XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

Acc65I       2338  GGTACC               G^GTACC
AflIII       1181  ACATGT               A^C[GA][CT]GT
AhdI         2190  GACAGCTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I        306  GTGCAC               G^TGCAC
ApaLI         306  GTGCAC               G^TGCAC
Asp718I      2338  GGTACC               G^GTACC
AspEI        2190  GACAGCTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI        1099  GGATCC               G^GATCC
BbvCI        1553  CCTCAGC              CCTCAGC(-5/-2)
BsaWI        2509  ACCGGA               [AT]^CCGG[AT]
BsiEI        5736  CGGCCG               CG[GA][CT]^CG
BspDI        1654  ATCGAT               AT^CGAT
BspLU11I     1181  ACATGT               A^CATGT
BsrDI        5686  CATTGC               GCAATG(2/0)
BssHII       5523  GCGCGC               G^CGCGC
BssSI        5609  CTCGTG               CACGAG(-5/-1)
BstBI         729  TTCGAA               TT^CGAA
ClaI         1654  ATCGAT               AT^CGAT
DrdI          832  GACAACTGTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI         5736  CGGCCG               C^GGCCG
Eam1105I     2190  GACAGCTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI        5736  CGGCCG               C^GGCCG
Eco52I       5736  CGGCCG               C^GGCCG
EcoRV        1651  GATATC               GAT^ATC
KpnI         2338  GGTACC               GGTAC^C
MseI          497  TTAA                 T^TAA
NheI         5400  GCTAGC               G^CTAGC
NspV          729  TTCGAA               TT^CGAA
SacI         4436  GAGCTC               GAGCT^C
SapI         4390  GCTCTTC              GCTCTTC(1/4)
ScaI         3770  AGTACT               AGT^ACT
SexAI        5144  ACCTGGT              A^CC[AT]GGT
SfuI          729  TTCGAA               TT^CGAA
SgrAI        5405  CGCCGGCG             C[GA]^CCGG[CT]G
Tru9I         497  TTAA                 T^TAA
Tsp509I      2566  AATT                 ^AATT


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AatII        2686  GACGTC               GACGT^C
             5413  GACGTC              
BalI          515  TGGCCA               TGG^CCA
             3077  TGGCCA              
BbrPI        2788  CACGTG               CAC^GTG
             4641  CACGTG              
BclI         1422  TGATCA               T^GATCA
             3780  TGATCA              
BsaAI        2788  CACGTG               [CT]AC^GT[GA]
             4641  CACGTG              
BsrFI        4499  GCCGGC               [GA]^CCGG[CT]
             5406  GCCGGC              
Cfr10I       4499  GCCGGC               [GA]^CCGG[CT]
             5406  GCCGGC              
FbaI         1422  TGATCA               T^GATCA
             3780  TGATCA              
HaeII        4677  GGCGCT               [GA]GCGC^[CT]
             5403  AGCGCC              
MluNI         515  TGGCCA               TGG^CCA
             3077  TGGCCA              
MscI          515  TGGCCA               TGG^CCA
             3077  TGGCCA              
NaeI         4499  GCCGGC               GCC^GGC
             5406  GCCGGC              
NgoMIV       4499  GCCGGC               G^CCGGC
             5406  GCCGGC              
PflMI        1290  CCAGGACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3069  CCACTCTCTGG         
PmaCI        2788  CACGTG               CAC^GTG
             4641  CACGTG              
PmlI         2788  CACGTG               CAC^GTG
             4641  CACGTG              
SalI         2722  GTCGAC               G^TCGAC
             4418  GTCGAC              
SmlI         4531  CTCAAG               C^T[CT][GA]AG
             4960  CTTGAG              
SphI          434  GCATGC               GCATG^C
             5756  GCATGC              
Van91I       1290  CCAGGACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3069  CCACTCTCTGG         


#########################################
# 3 OR MORE CUT ENZYMES (  127 enzymes) #
#########################################

AccI        AccII       AciI        AcyI        AfaI        
AluI        AlwI        AlwNI       AocI        ApaI        
AspHI       AspI        AvaI        AvaII       AvrII       
BanI        BanII       BbsI        BbvI        BcgI        
BcnI        BfaI        BglI        BlnI        BmyI        
BpmI        BpuAI       BsaHI       BsaI        BsaJI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmBI       BsmFI       BsoBI       Bsp1286I    
BspMI       BsrBI       BsrI        BssKI       BstAPI      
BstF5I      BstNI       BstUI       BstXI       BstYI       
Bsu36I      Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraII       DraIII      DsaI        EaeI        
EarI        Eco81I      EcoNI       EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NcoI        NdeII       NlaIII      NlaIV       
NspI        PflFI       PleI        PpuMI       PshAI       
PstI        PvuII       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmaI        StuI        
StyI        TaqI        TfiI        TseI        Tsp45I      
TspRI       Tth111I     TthHB8I     XcmI        XhoII       
XmaCI       XmaI        
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