Restriction map for pF1KE2186
##################################
# NO CUT ENZYMES (  101 enzymes) #
##################################

AatII       AccIII      AclI        AcsI        AflII       
AflIII      AgeI        AhdI        AocI        Aor51HI     
ApoI        AscI        AseI        AsnI        Asp700I     
AspEI       AspI        AviII       AvrII       BamHI       
BbvCI       BcgI        BciVI       BclI        BfaI        
BfrI        BglII       BlnI        BsaWI       BseAI       
BseRI       BsiWI       BsmBI       BspDI       BspEI       
BspLU11I    BsrDI       BssSI       Bst1107I    BstBI       
BstEII      BstPI       BstZ17I     Bsu36I      ClaI        
CpoI        DraI        DraIII      Eam1105I    Eco47III    
Eco81I      EcoNI       EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        FseI        FspI        HincII      
HindII      HindIII     HpaI        MaeI        MfeI        
MluI        MroI        MseI        MunI        NdeI        
NheI        NsiI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmeI        PshAI       PshBI       
Psp1406I    PvuI        RsrII       SalI        SbfI        
SfiI        SfuI        SgrAI       SnaBI       SpeI        
SphI        Sse8387I    SspI        SwaI        TfiI        
Tru9I       Tsp509I     Tth111I     XbaI        XhoI        
XmnI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

Acc65I        657  GGTACC               G^GTACC
AccI          964  GTCTAC               GT^[AC][GT]AC
Asp718I       657  GGTACC               G^GTACC
BbrPI        1750  CACGTG               CAC^GTG
BbsI          952  GTCTTC               GAAGAC(2/6)
BlpI         1131  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     1131  GCTGAGC              GC^T[ACGT]AGC
BpuAI         952  GTCTTC               GAAGAC(2/6)
BsaBI        1341  GATCCGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI          922  GAGACC               GGTCTC(1/5)
BsgI          935  CTGCAC               GTGCAG(16/14)
BsmAI         922  GAGAC                GTCTC(1/5)
BsmI          541  GAATGC               GAATGC(1/-1)
Bsp1407I      266  TGTACA               T^GTACA
BspHI        1337  TCATGA               T^CATGA
BsrGI         266  TGTACA               T^GTACA
BstAPI        389  GCATGAGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII        1131  GCTGAGC              GC^T[ACGT]AGC
EagI           71  CGGCCG               C^GGCCG
EclXI          71  CGGCCG               C^GGCCG
Eco52I         71  CGGCCG               C^GGCCG
KpnI          657  GGTACC               GGTAC^C
MaeIII        606  GTCAC                ^GT[ACGT]AC
MamI         1341  GATCCGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI         1649  CCATGG               C^CATGG
NotI           70  GCGGCCGC             GC^GGCCGC
NruI          576  TCGCGA               TCG^CGA
NspI          149  ACATGC               [GA]CATG^[CT]
PmaCI        1750  CACGTG               CAC^GTG
PmlI         1750  CACGTG               CAC^GTG
PstI          891  CTGCAG               CTGCA^G
PvuII          55  CAGCTG               CAG^CTG
RcaI         1337  TCATGA               T^CATGA
SacI         1134  GAGCTC               GAGCT^C
SexAI        1015  ACCTGGT              A^CC[AT]GGT
SfcI          891  CTGCAG               C^T[GA][CT]AG
SmaI         1458  CCCGGG               CCC^GGG
SmlI         1513  CTCAAG               C^T[CT][GA]AG
SspBI         266  TGTACA               T^GTACA
TaqI          776  TCGA                 T^CGA
Tsp45I        606  GTCAC                ^GT[GC]AC
TthHB8I       776  TCGA                 T^CGA
XmaCI        1458  CCCGGG               C^CCGGG
XmaI         1458  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

Alw44I        199  GTGCAC               G^TGCAC
              942  GTGCAC              
AlwNI        1183  CAGAACCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1602  CAGCCGCTG           
ApaLI         199  GTGCAC               G^TGCAC
              942  GTGCAC              
BalI          761  TGGCCA               TGG^CCA
              980  TGGCCA              
BpmI          193  CTGGAG               CTGGAG(16/14)
             1327  CTGGAG              
BsaAI        1171  TACGTG               [CT]AC^GT[GA]
             1750  CACGTG              
BsiEI          71  CGGCCG               CG[GA][CT]^CG
              399  CGACCG              
BssHII        330  GCGCGC               G^CGCGC
             1482  GCGCGC              
BstXI         752  CCACCACAGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1382  CCAGCATTGTGG        
BstYI         224  AGATCC               [GA]^GATC[CT]
             1559  GGATCT              
DdeI          800  CTGAG                C^T[ACGT]AG
             1132  CTGAG               
DrdI          424  GACGCCGAGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1575  GACGGTGGAGTC        
EarI         1249  CTCTTC               CTCTTC(1/4)
             1276  CTCTTC              
HinfI         604  GAGTC                G^A[ACGT]TC
             1582  GAGTC               
Ksp632I      1249  CTCTTC               CTCTTC(1/4)
             1276  CTCTTC              
KspI          123  CCGCGG               CCGC^GG
             1621  CCGCGG              
MflI          224  AGATCC               [GA]^GATC[CT]
             1559  GGATCT              
MluNI         761  TGGCCA               TGG^CCA
              980  TGGCCA              
MscI          761  TGGCCA               TGG^CCA
              980  TGGCCA              
PflMI         704  CCACCTTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              989  CCATCTGGTGG         
PleI          604  GAGTC                GAGTC(4/5)
             1582  GAGTC               
PpuMI         431  AGGTCCT              [GA]G^G[AT]CC[CT]
             1148  GGGACCC             
SacII         123  CCGCGG               CCGC^GG
             1621  CCGCGG              
SapI         1248  GCTCTTC              GCTCTTC(1/4)
             1275  GCTCTTC             
StuI          481  AGGCCT               AGG^CCT
              717  AGGCCT              
Van91I        704  CCACCTTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              989  CCATCTGGTGG         
XcmI         1115  CCATCACGGCACTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1382  CCAGCATTGTGGTGG     
XhoII         224  AGATCC               [GA]^GATC[CT]
             1559  GGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   85 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        ApaI        AspHI       AvaI        AvaII       
BanI        BanII       BbeI        BbvI        BcnI        
BglI        BmyI        BsaHI       BsaJI       BsiHKAI     
BsiYI       BslI        BsmFI       BsoBI       Bsp1286I    
BspMI       BsrBI       BsrFI       BsrI        BssKI       
BstF5I      BstNI       BstUI       Cac8I       CfoI        
Cfr10I      Cfr13I      DpnII       DraII       DsaI        
EaeI        EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HpaII       
HphI        ItaI        KasI        MaeII       MboI        
MboII       MnlI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NaeI        NarI        
NciI        NdeII       NgoMIV      NlaIII      NlaIV       
RsaI        Sau3AI      Sau96I      ScaI        ScrFI       
SfaNI       SfoI        StyI        TseI        TspRI       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com