Restriction map for pF1KE2186
 (Linear) (Six-base) MAPSORT of: KIEE2186.seq  Check: 140  from: 1  to: 1755



KIEE2186.orf

 With 182 enzymes: SgfI * 

                        July 16, 2019 14:21  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1456    1725    1755 
   Size:       1456     269      30

  Fragments arranged by size: 

                1456     269      30
 
AccI GT'mk_AC

Cuts at:      0     965    1755 
   Size:        965     790
 
Acc65I G'GTAC_C

Cuts at:      0     657    1755 
   Size:        657    1098
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     619     663    1755 
   Size:        619      44    1092

  Fragments arranged by size: 

                1092     619      44
 
AleI CACnn'nnGTG

Cuts at:      0     757    1755 
   Size:        757     998
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1188    1607    1755 
   Size:       1188     419     148

  Fragments arranged by size: 

                1188     419     148
 
ApaI G_GGCC'C

Cuts at:      0     559     934    1219    1714    1755 
   Size:        559     375     285     495      41

  Fragments arranged by size: 

                 559     495     375     285      41
 
ApaLI G'TGCA_C

Cuts at:      0     199     942    1755 
   Size:        199     743     813

  Fragments arranged by size: 

                 813     743     199
 
AvaI C'yCGr_G

Cuts at:      0     382     531    1458    1492    1669    1755 
   Size:        382     149     927      34     177      86

  Fragments arranged by size: 

                 927     382     177     149      86      34
 
BanI G'GyrC_C

Cuts at:      0     111     471     522     642     657    1309    1645    1755 
   Size:        111     360      51     120      15     652     336     110

  Fragments arranged by size: 

                 652     360     336     120     111     110      51      15
            
 
BanII G_rGCy'C

Cuts at:      0     559     584     934    1138    1219    1714    1755 
   Size:        559      25     350     204      81     495      41

  Fragments arranged by size: 

                 559     495     350     204      81      41      25
 
BbeI G_GCGC'C

Cuts at:      0     475     526    1649    1755 
   Size:        475      51    1123     106

  Fragments arranged by size: 

                1123     475     106      51
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     945    1755 
   Size:        945     810
 
BglI GCCn_nnn'nGGC

Cuts at:      0     368     469     480     886    1755 
   Size:        368     101      11     406     869

  Fragments arranged by size: 

                 869     406     368     101      11
 
BlpI GC'TnA_GC

Cuts at:      0    1132    1755 
   Size:       1132     623
 
Bme1580I G_kGCm'C

Cuts at:      0     116     203     559     593     647     934     946    1219 
   Size:        116      87     356      34      54     287      12     273

Cuts at:   1219    1246    1714    1755 
   Size:         27     468      41

  Fragments arranged by size: 

                 468     356     287     273     116      87      54      41
                  34      27      12
 
BmgBI CAC'GTC

Cuts at:      0     749    1547    1755 
   Size:        749     798     208

  Fragments arranged by size: 

                 798     749     208
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1147    1509    1755 
   Size:       1147     362     246

  Fragments arranged by size: 

                1147     362     246
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     214    1348    1755 
   Size:        214    1134     407

  Fragments arranged by size: 

                1134     407     214
 
Bpu10I CC'TnA_GC

Cuts at:      0     800    1755 
   Size:        800     955
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1498    1755 
   Size:       1498     257
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     916    1755 
   Size:        916     839
 
BsaAI yAC'GTr

Cuts at:      0    1173    1752    1755 
   Size:       1173     579       3

  Fragments arranged by size: 

                1173     579       3
 
BsaBI GATnn'nnATC

Cuts at:      0    1345    1755 
   Size:       1345     410
 
BsaHI Gr'CG_yC

Cuts at:      0     425     472     523    1553    1646    1755 
   Size:        425      47      51    1030      93     109

  Fragments arranged by size: 

                1030     425     109      93      51      47
 
BseYI C'CCAG_C

Cuts at:      0     414     489     550     846    1102    1212    1430    1755 
   Size:        414      75      61     296     256     110     218     325

  Fragments arranged by size: 

                 414     325     296     256     218     110      75      61
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     920    1755 
   Size:        920     835
 
BsiEI CG_ry'CG

Cuts at:      0      74     402    1755 
   Size:         74     328    1353

  Fragments arranged by size: 

                1353     328      74
 
BsiHKAI G_wGCw'C

Cuts at:      0     203     905     946    1138    1229    1508    1755 
   Size:        203     702      41     192      91     279     247

  Fragments arranged by size: 

                 702     279     247     203     192      91      41
 
BsmI GAATG_Cn'

Cuts at:      0     547    1755 
   Size:        547    1208
 
Bsp1286I G_dGCh'C

Cuts at:      0     116     203     559     584     593     647     905     934 
   Size:        116      87     356      25       9      54     258      29

Cuts at:    934     946    1138    1219    1229    1246    1508    1714    1755 
   Size:         12     192      81      10      17     262     206      41

  Fragments arranged by size: 

                 356     262     258     206     192     116      87      81
                  54      41      29      25      17      12      10       9
            
 
BspHI T'CATG_A

Cuts at:      0    1337    1755 
   Size:       1337     418
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     251    1241    1456    1725    1755 
   Size:        251     990     215     269      30

  Fragments arranged by size: 

                 990     269     251     215      30
 
BsrBI CCG'CTC

Cuts at:      0     317     615    1640    1755 
   Size:        317     298    1025     115

  Fragments arranged by size: 

                1025     317     298     115
 
BsrFI r'CCGG_y

Cuts at:      0     340     525    1030    1159    1471    1755 
   Size:        340     185     505     129     312     284

  Fragments arranged by size: 

                 505     340     312     284     185     129
 
BsrGI T'GTAC_A

Cuts at:      0     266    1755 
   Size:        266    1489
 
BssHII G'CGCG_C

Cuts at:      0     330    1482    1755 
   Size:        330    1152     273

  Fragments arranged by size: 

                1152     330     273
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     395    1755 
   Size:        395    1360
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     759    1389    1755 
   Size:        759     630     366

  Fragments arranged by size: 

                 759     630     366
 
BstYI r'GATC_y

Cuts at:      0     224    1559    1755 
   Size:        224    1335     196

  Fragments arranged by size: 

                1335     224     196
 
BtgI C'CryG_G

Cuts at:      0     123     467     926    1139    1621    1649    1755 
   Size:        123     344     459     213     482      28     106

  Fragments arranged by size: 

                 482     459     344     213     123     106      28
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1041    1755 
   Size:       1041     714
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     430    1581    1755 
   Size:        430    1151     174

  Fragments arranged by size: 

                1151     430     174
 
EaeI y'GGCC_r

Cuts at:      0      71     211     761     980    1025    1157    1473    1629 
   Size:         71     140     550     219      45     132     316     156

Cuts at:   1629    1652    1755 
   Size:         23     103

  Fragments arranged by size: 

                 550     316     219     156     140     132     103      71
                  45      23
 
EagI C'GGCC_G

Cuts at:      0      71    1755 
   Size:         71    1684
 
EarI CTCTTCn'nnn_

Cuts at:      0    1255    1282    1755 
   Size:       1255      27     473

  Fragments arranged by size: 

                1255     473      27
 
EcoICRI GAG'CTC

Cuts at:      0    1136    1755 
   Size:       1136     619
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     214     619     663    1348    1755 
   Size:        214     405      44     685     407

  Fragments arranged by size: 

                 685     407     405     214      44
 
EcoO109I rG'GnC_Cy

Cuts at:      0      87     432     555     931    1149    1711    1755 
   Size:         87     345     123     376     218     562      44

  Fragments arranged by size: 

                 562     376     345     218     123      87      44
 
HaeII r_GCGC'y

Cuts at:      0     475     526     807    1132    1444    1649    1682    1755 
   Size:        475      51     281     325     312     205      33      73

  Fragments arranged by size: 

                 475     325     312     281     205      73      51      33
            
 
KasI G'GCGC_C

Cuts at:      0     471     522    1645    1755 
   Size:        471      51    1123     110

  Fragments arranged by size: 

                1123     471     110      51
 
KpnI G_GTAC'C

Cuts at:      0     661    1755 
   Size:        661    1094
 
MscI TGG'CCA

Cuts at:      0     763     982    1755 
   Size:        763     219     773

  Fragments arranged by size: 

                 773     763     219
 
MslI CAynn'nnrTG

Cuts at:      0     757     778    1390    1755 
   Size:        757      21     612     365

  Fragments arranged by size: 

                 757     612     365      21
 
MspA1I CmG'CkG

Cuts at:      0      57     125     216     303     354     550    1221    1607 
   Size:         57      68      91      87      51     196     671     386

Cuts at:   1607    1623    1755 
   Size:         16     132

  Fragments arranged by size: 

                 671     386     196     132      91      87      68      57
                  51      16
 
NaeI GCC'GGC

Cuts at:      0     342     527    1032    1161    1755 
   Size:        342     185     505     129     594

  Fragments arranged by size: 

                 594     505     342     185     129
 
NarI GG'CG_CC

Cuts at:      0     472     523    1646    1755 
   Size:        472      51    1123     109

  Fragments arranged by size: 

                1123     472     109      51
 
NcoI C'CATG_G

Cuts at:      0    1649    1755 
   Size:       1649     106
 
NgoMIV G'CCGG_C

Cuts at:      0     340     525    1030    1159    1755 
   Size:        340     185     505     129     596

  Fragments arranged by size: 

                 596     505     340     185     129
 
NotI GC'GGCC_GC

Cuts at:      0      71    1755 
   Size:         71    1684
 
NruI TCG'CGA

Cuts at:      0     578    1755 
   Size:        578    1177
 
NspI r_CATG'y

Cuts at:      0     153    1755 
   Size:        153    1602
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     710     995    1755 
   Size:        710     285     760

  Fragments arranged by size: 

                 760     710     285
 
PmlI CAC'GTG

Cuts at:      0    1752    1755 
   Size:       1752       3
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     682     714    1177    1209    1755 
   Size:        682      32     463      32     546

  Fragments arranged by size: 

                 682     546     463      32      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     432    1149    1755 
   Size:        432     717     606

  Fragments arranged by size: 

                 717     606     432
 
PspOMI G'GGCC_C

Cuts at:      0     555     930    1215    1710    1755 
   Size:        555     375     285     495      45

  Fragments arranged by size: 

                 555     495     375     285      45
 
PstI C_TGCA'G

Cuts at:      0     895    1755 
   Size:        895     860
 
PvuII CAG'CTG

Cuts at:      0      57    1755 
   Size:         57    1698
 
SacI G_AGCT'C

Cuts at:      0    1138    1755 
   Size:       1138     617
 
SacII CC_GC'GG

Cuts at:      0     126    1624    1755 
   Size:        126    1498     131

  Fragments arranged by size: 

                1498     131     126
 
SanDI GG'GwC_CC

Cuts at:      0    1149    1755 
   Size:       1149     606
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1255    1282    1755 
   Size:       1255      27     473

  Fragments arranged by size: 

                1255     473      27
 
ScaI AGT'ACT

Cuts at:      0    1075    1498    1519    1755 
   Size:       1075     423      21     236

  Fragments arranged by size: 

                1075     423     236      21
 
SexAI A'CCwGG_T

Cuts at:      0    1015    1755 
   Size:       1015     740
 
SfcI C'TryA_G

Cuts at:      0     891    1755 
   Size:        891     864
 
SfoI GGC'GCC

Cuts at:      0     473     524    1647    1755 
   Size:        473      51    1123     108

  Fragments arranged by size: 

                1123     473     108      51
 
SmaI CCC'GGG

Cuts at:      0    1460    1755 
   Size:       1460     295
 
SmlI C'TyrA_G

Cuts at:      0    1513    1755 
   Size:       1513     242
 
SrfI GCCC'GGGC

Cuts at:      0    1460    1755 
   Size:       1460     295
 
StuI AGG'CCT

Cuts at:      0     483     719    1755 
   Size:        483     236    1036

  Fragments arranged by size: 

                1036     483     236
 
StyI C'CwwG_G

Cuts at:      0      83     335    1649    1755 
   Size:         83     252    1314     106

  Fragments arranged by size: 

                1314     252     106      83
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1491    1755 
   Size:       1491     264
 
TatI w'GTAC_w

Cuts at:      0     266    1073    1496    1517    1755 
   Size:        266     807     423      21     238

  Fragments arranged by size: 

                 807     423     266     238      21
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1122    1389    1755 
   Size:       1122     267     366

  Fragments arranged by size: 

                1122     366     267
 
XmaI C'CCGG_G

Cuts at:      0    1458    1755 
   Size:       1458     297
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AcuI     AleI    AlwNI     ApaI    ApaLI
     AvaI     BanI    BanII     BbeI     BbsI     BglI     BlpI Bme1580I
    BmgBI     BmrI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspHI
    BspMI    BsrBI    BsrFI    BsrGI   BssHII   BstAPI    BstXI    BstYI
     BtgI    BtgZI     DrdI     EaeI     EagI     EarI  EcoICRI  Eco57MI
 EcoO109I    HaeII     KasI     KpnI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI   NgoMIV     NotI     NruI     NspI    PflMI     PmlI
     PpiI    PpuMI   PspOMI     PstI    PvuII     SacI    SacII    SanDI
     SapI     ScaI    SexAI     SfcI     SfoI     SmaI     SmlI     SrfI
     StuI     StyI    TaqII     TatI     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AclI     AfeI    AflII   AflIII     AgeI     AhdI     AlfI
     AloI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI
    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII     BmtI     BplI
    BsaWI    BsaXI    BseRI    BsiWI    BsmBI    BspEI    BsrDI    BssSI
    BstBI   BstEII  BstZ17I   Bsu36I     BtsI     ClaI    CspCI     DraI
   DraIII     EciI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    Hin4I   HincII  HindIII     HpaI     MfeI     MluI     MmeI
     NdeI     NheI     NsiI     PacI     PasI     PciI     PfoI     PmeI
    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII     SalI     SbfI
     SfiI     SgfI    SgrAI    SnaBI     SpeI     SphI     SspI     SwaI
     TstI  Tth111I     XbaI     XhoI     XmnI     ZraI
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