Restriction map for pF1KE2065
##################################
# NO CUT ENZYMES (  124 enzymes) #
##################################

AccI        AccII       AclI        AcsI        AflIII      
AgeI        AhdI        Alw44I      AocI        Aor51HI     
ApaI        ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     AspEI       AspI        AviII       
AvrII       BbeI        BbrPI       BbvCI       BciVI       
BglI        BglII       BlnI        BsaAI       BsiEI       
BsiWI       BsmBI       BsmI        Bsp1407I    BspDI       
BspHI       BspLU11I    BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstUI       BstXI       BstZ17I     Bsu36I      
CfoI        Cfr10I      ClaI        CpoI        DraI        
DraII       DrdI        DsaI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoO109I    
EcoO65I     EcoRI       EcoRV       EcoT22I     FauI        
FseI        FspI        HaeII       HhaI        HinP1I      
HindIII     KasI        KspI        MfeI        MluI        
MunI        MvnI        NaeI        NarI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacII       SalI        SapI        ScaI        
SfiI        SfoI        SfuI        SgrAI       SnaBI       
SpeI        SphI        SspBI       SspI        SwaI        
Tth111I     XbaI        XhoI        XmnI        


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# ONE CUT ENZYMES (   44 enzymes) #
###################################

AatII         127  GACGTC               GACGT^C
Acc65I        223  GGTACC               G^GTACC
AccIII       1775  TCCGGA               T^CCGGA
AflII         204  CTTAAG               C^TTAAG
Asp718I       223  GGTACC               G^GTACC
AspHI         484  GAGCTC               G[AT]GC[AT]^C
BalI          170  TGGCCA               TGG^CCA
BbsI         1209  GAAGAC               GAAGAC(2/6)
BcgI         1500  CGAGGTTTATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI          761  TGATCA               T^GATCA
BfrI          204  CTTAAG               C^TTAAG
BlpI         1392  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     1392  GCTGAGC              GC^T[ACGT]AGC
BpuAI        1209  GAAGAC               GAAGAC(2/6)
BsaBI        1222  GATGCCGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI        1775  TCCGGA               [AT]^CCGG[AT]
BseAI        1775  TCCGGA               T^CCGGA
BsiHKAI       484  GAGCTC               G[AT]GC[AT]^C
BspEI        1775  TCCGGA               T^CCGGA
BspMI         984  ACCTGC               ACCTGC(4/8)
BsrBI        1279  GAGCGG               CCGCTC(-3/-3)
CelII        1392  GCTGAGC              GC^T[ACGT]AGC
DraIII        330  CACGAAGTG            CAC[ACGT][ACGT][ACGT]^GTG
FbaI          761  TGATCA               T^GATCA
HgaI          740  GCGTC                GACGC(5/10)
HpaI         1108  GTTAAC               GTT^AAC
KpnI          223  GGTACC               GGTAC^C
MamI         1222  GATGCCGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI         170  TGGCCA               TGG^CCA
MroI         1775  TCCGGA               T^CCGGA
MscI          170  TGGCCA               TGG^CCA
MslI          384  CATCCAGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NlaIII       1306  CATG                 CATG^
PleI           13  GAGTC                GAGTC(4/5)
PshAI         568  GACAACCGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI          484  GAGCTC               GAGCT^C
SbfI          132  CCTGCAGG             CCTGCA^GG
SexAI         949  ACCTGGT              A^CC[AT]GGT
SmaI         1545  CCCGGG               CCC^GGG
SmlI          204  CTTAAG               C^T[CT][GA]AG
Sse8387I      132  CCTGCAGG             CCTGCA^GG
StuI         1894  AGGCCT               AGG^CCT
XmaCI        1545  CCCGGG               C^CCGGG
XmaI         1545  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AcyI          127  GACGTC               G[GA]^CG[CT]C
              739  GGCGTC              
AvaI         1498  CCCGAG               C^[CT]CG[GA]G
             1545  CCCGGG              
BamHI         876  GGATCC               G^GATCC
             2081  GGATCC              
BanI           30  GGTGCC               G^G[CT][GA]CC
              223  GGTACC              
BanII         484  GAGCTC               G[GA]GC[CT]^C
             1513  GGGCTC              
BfaI         1087  CTAG                 C^TAG
             2078  CTAG                
BmyI          484  GAGCTC               G[AGT]GC[ACT]^C
             1513  GGGCTC              
BsaHI         127  GACGTC               G[GA]^CG[CT]C
              739  GGCGTC              
BsaI          820  GAGACC               GGTCTC(1/5)
             1852  GAGACC              
BsoBI        1498  CCCGAG               C^[CT]CG[GA]G
             1545  CCCGGG              
Bsp1286I      484  GAGCTC               G[AGT]GC[ACT]^C
             1513  GGGCTC              
BsrDI         729  CATTGC               GCAATG(2/0)
              924  GCAATG              
EaeI          170  TGGCCA               [CT]^GGCC[GA]
              428  TGGCCG              
EcoNI         156  CCTGGTGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1322  CCTTTTGGAGG         
Hin1I         127  GACGTC               G[GA]^CG[CT]C
              739  GGCGTC              
MaeI         1087  CTAG                 C^TAG
             2078  CTAG                
PflMI         464  CCAGCCCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1035  CCACCTTCTGG         
TfiI          410  GAATC                G^A[AT]TC
             2126  GAATC               
Van91I        464  CCAGCCCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1035  CCACCTTCTGG         
XcmI         1922  CCACAGGTGACGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2026  CCAATACCACCTTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   70 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AlwNI       
AvaII       BbvI        BcnI        BpmI        BsaJI       
BseRI       BsgI        BsiYI       BslI        BsmAI       
BsmFI       BsrI        BssKI       BstF5I      BstNI       
BstYI       Cac8I       Cfr13I      DdeI        DpnII       
EarI        EcoRII      EcoT14I     Fnu4HI      FokI        
HaeIII      HapII       HincII      HindII      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MspA1I      MspI        MvaI        MwoI        
NciI        NdeII       NlaIV       PstI        PvuII       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        StyI        TaqI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     XhoII       
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