Restriction map for pF1KE3822
 (Linear) (Six-base) MAPSORT of: KIEE3822.seq  Check: 6022  from: 1  to: 7581



KIEE3822.orf

 With 182 enzymes: SgfI * 

                        October 24, 2019 20:50  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     225    7581 
   Size:        225    7356
 
AccI GT'mk_AC

Cuts at:      0    7076    7326    7581 
   Size:       7076     250     255

  Fragments arranged by size: 

                7076     255     250
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      67     416     427    1388    2615    2846    2857    3121 
   Size:         67     349      11     961    1227     231      11     264

Cuts at:   3121    3403    3412    4298    5252    5276    5860    6143    6578 
   Size:        282       9     886     954      24     584     283     435

Cuts at:   6578    7240    7460    7581 
   Size:        662     220     121

  Fragments arranged by size: 

                1227     961     954     886     662     584     435     349
                 283     282     264     231     220     121      67      24
                  11      11       9
 
AfeI AGC'GCT

Cuts at:      0     935    7581 
   Size:        935    6646
 
AflII C'TTAA_G

Cuts at:      0    2370    3663    7581 
   Size:       2370    1293    3918

  Fragments arranged by size: 

                3918    2370    1293
 
AflIII A'CryG_T

Cuts at:      0     552    4934    7335    7581 
   Size:        552    4382    2401     246

  Fragments arranged by size: 

                4382    2401     552     246
 
AleI CACnn'nnGTG

Cuts at:      0     252    3310    7581 
   Size:        252    3058    4271

  Fragments arranged by size: 

                4271    3058     252
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    5936    7581 
   Size:       5936    1645
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    3538    3570    7581 
   Size:       3538      32    4011

  Fragments arranged by size: 

                4011    3538      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1812    7581 
   Size:       1812    5769
 
ApaI G_GGCC'C

Cuts at:      0    2813    7581 
   Size:       2813    4768
 
ApaLI G'TGCA_C

Cuts at:      0    6116    7168    7581 
   Size:       6116    1052     413

  Fragments arranged by size: 

                6116    1052     413
 
ApoI r'AATT_y

Cuts at:      0     592     639     794    1573    1802    1886    2586    2611 
   Size:        592      47     155     779     229      84     700      25

Cuts at:   2611    2743    2781    3136    3486    3550    4198    4630    4897 
   Size:        132      38     355     350      64     648     432     267

Cuts at:   4897    4918    4963    5626    6396    6780    6921    7515    7581 
   Size:         21      45     663     770     384     141     594      66

  Fragments arranged by size: 

                 779     770     700     663     648     594     592     432
                 384     355     350     267     229     155     141     132
                  84      66      64      47      45      38      25      21
            
 
AseI AT'TA_AT

Cuts at:      0     262    4060    5122    6328    7581 
   Size:        262    3798    1062    1206    1253

  Fragments arranged by size: 

                3798    1253    1206    1062     262
 
AvrII C'CTAG_G

Cuts at:      0    5652    7581 
   Size:       5652    1929
 
BanII G_rGCy'C

Cuts at:      0    2813    7581 
   Size:       2813    4768
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     415    2030    7581 
   Size:        415    1615    5551

  Fragments arranged by size: 

                5551    1615     415
 
BbvCI CC'TCA_GC

Cuts at:      0    4261    7581 
   Size:       4261    3320
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1560    1796    1825    6026    6556    7581 
   Size:       1560     236      29    4201     530    1025

  Fragments arranged by size: 

                4201    1560    1025     530     236      29
 
BclI T'GATC_A

Cuts at:      0    2151    2528    2820    7581 
   Size:       2151     377     292    4761

  Fragments arranged by size: 

                4761    2151     377     292
 
BfrBI ATG'CAT

Cuts at:      0    1221    1261    1726    6461    7581 
   Size:       1221      40     465    4735    1120

  Fragments arranged by size: 

                4735    1221    1120     465      40
 
BglII A'GATC_T

Cuts at:      0     417     926    4080    4158    5511    7581 
   Size:        417     509    3154      78    1353    2070

  Fragments arranged by size: 

                3154    2070    1353     509     417      78
 
BlpI GC'TnA_GC

Cuts at:      0     937    6603    7097    7581 
   Size:        937    5666     494     484

  Fragments arranged by size: 

                5666     937     494     484
 
Bme1580I G_kGCm'C

Cuts at:      0    1119    2813    6103    6120    6306    6518    7172    7581 
   Size:       1119    1694    3290      17     186     212     654     409

  Fragments arranged by size: 

                3290    1694    1119     654     409     212     186      17
            
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    4037    5783    7581 
   Size:       4037    1746    1798

  Fragments arranged by size: 

                4037    1798    1746
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     168     588    5865    6550    7581 
   Size:        168     420    5277     685    1031

  Fragments arranged by size: 

                5277    1031     685     420     168
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     136    1525    3067    3346    5407    5624    6227    7307 
   Size:        136    1389    1542     279    2061     217     603    1080

Cuts at:   7307    7581 
   Size:        274

  Fragments arranged by size: 

                2061    1542    1389    1080     603     279     274     217
                 136
 
Bpu10I CC'TnA_GC

Cuts at:      0    2998    4261    7581 
   Size:       2998    1263    3320

  Fragments arranged by size: 

                3320    2998    1263
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    3396    3466    3729    5158    6341    7581 
   Size:       3396      70     263    1429    1183    1240

  Fragments arranged by size: 

                3396    1429    1240    1183     263      70
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    5231    5972    7581 
   Size:       5231     741    1609

  Fragments arranged by size: 

                5231    1609     741
 
BsaAI yAC'GTr

Cuts at:      0     137    1095    5931    7336    7581 
   Size:        137     958    4836    1405     245

  Fragments arranged by size: 

                4836    1405     958     245     137
 
BsaBI GATnn'nnATC

Cuts at:      0    2204    3697    7581 
   Size:       2204    1493    3884

  Fragments arranged by size: 

                3884    2204    1493
 
BsaWI w'CCGG_w

Cuts at:      0    4646    7427    7581 
   Size:       4646    2781     154

  Fragments arranged by size: 

                4646    2781     154
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    5690    5720    7581 
   Size:       5690      30    1861

  Fragments arranged by size: 

                5690    1861      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     412     592     943    7304    7581 
   Size:        412     180     351    6361     277

  Fragments arranged by size: 

                6361     412     351     277     180
 
BseYI C'CCAG_C

Cuts at:      0    1112    1185    4761    5812    6504    7581 
   Size:       1112      73    3576    1051     692    1077

  Fragments arranged by size: 

                3576    1112    1077    1051     692      73
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     250     916    1117    1519    7581 
   Size:        250     666     201     402    6062

  Fragments arranged by size: 

                6062     666     402     250     201
 
BsiHKAI G_wGCw'C

Cuts at:      0     149    5178    6120    7172    7581 
   Size:        149    5029     942    1052     409

  Fragments arranged by size: 

                5029    1052     942     409     149
 
BsmI GAATG_Cn'

Cuts at:      0     775     918    1221    1263    2182    2907    3066    4094 
   Size:        775     143     303      42     919     725     159    1028

Cuts at:   4094    4470    6463    6495    7581 
   Size:        376    1993      32    1086

  Fragments arranged by size: 

                1993    1086    1028     919     775     725     376     303
                 159     143      42      32
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    6417    7557    7581 
   Size:       6417    1140      24

  Fragments arranged by size: 

                6417    1140      24
 
Bsp1286I G_dGCh'C

Cuts at:      0     149    1119    2813    5178    6103    6120    6306    6518 
   Size:        149     970    1694    2365     925      17     186     212

Cuts at:   6518    7172    7581 
   Size:        654     409

  Fragments arranged by size: 

                2365    1694     970     925     654     409     212     186
                 149      17
 
BspHI T'CATG_A

Cuts at:      0     725    1479    2823    7370    7581 
   Size:        725     754    1344    4547     211

  Fragments arranged by size: 

                4547    1344     754     725     211
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     225    4177    5597    7581 
   Size:        225    3952    1420    1984

  Fragments arranged by size: 

                3952    1984    1420     225
 
BsrDI GCAATG_nn'

Cuts at:      0     741     919    3621    6032    6056    7581 
   Size:        741     178    2702    2411      24    1525

  Fragments arranged by size: 

                2702    2411    1525     741     178      24
 
BsrGI T'GTAC_A

Cuts at:      0    1710    7581 
   Size:       1710    5871
 
BssSI C'ACGA_G

Cuts at:      0    2297    3069    7581 
   Size:       2297     772    4512

  Fragments arranged by size: 

                4512    2297     772
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    4391    6043    7581 
   Size:       4391    1652    1538

  Fragments arranged by size: 

                4391    1652    1538
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     313     535    2049    2271    2805    3861    7581 
   Size:        313     222    1514     222     534    1056    3720

  Fragments arranged by size: 

                3720    1514    1056     534     313     222     222
 
BstYI r'GATC_y

Cuts at:      0     120     417     926    2221    2293    3228    3844    3885 
   Size:        120     297     509    1295      72     935     616      41

Cuts at:   3885    4080    4158    4535    5251    5511    7311    7581 
   Size:        195      78     377     716     260    1800     270

  Fragments arranged by size: 

                1800    1295     935     716     616     509     377     297
                 270     260     195     120      78      72      41
 
Bsu36I CC'TnA_GG

Cuts at:      0    2571    4865    7581 
   Size:       2571    2294    2716

  Fragments arranged by size: 

                2716    2571    2294
 
BtgI C'CryG_G

Cuts at:      0    6307    7581 
   Size:       6307    1274
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    2594    7581 
   Size:       2594    4987
 
BtsI GCAGTG_nn'

Cuts at:      0    1451    1774    4765    7079    7265    7581 
   Size:       1451     323    2991    2314     186     316

  Fragments arranged by size: 

                2991    2314    1451     323     316     186
 
ClaI AT'CG_AT

Cuts at:      0    5130    7581 
   Size:       5130    2451
 
DraI TTT'AAA

Cuts at:      0     645    3401    3578    3924    7581 
   Size:        645    2756     177     346    3657

  Fragments arranged by size: 

                3657    2756     645     346     177
 
DraIII CAC_nnn'GTG

Cuts at:      0    4461    5997    7581 
   Size:       4461    1536    1584

  Fragments arranged by size: 

                4461    1584    1536
 
EaeI y'GGCC_r

Cuts at:      0    1176    2393    7581 
   Size:       1176    1217    5188

  Fragments arranged by size: 

                5188    1217    1176
 
EarI CTCTTCn'nnn_

Cuts at:      0      23     647    2646    3097    4309    7581 
   Size:         23     624    1999     451    1212    3272

  Fragments arranged by size: 

                3272    1999    1212     624     451      23
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      67     136     416     427    1388    1525    2615    2846 
   Size:         67      69     280      11     961     137    1090     231

Cuts at:   2846    2857    3067    3121    3346    3403    3412    4298    5252 
   Size:         11     210      54     225      57       9     886     954

Cuts at:   5252    5276    5407    5624    5860    6143    6227    6578    7240 
   Size:         24     131     217     236     283      84     351     662

Cuts at:   7240    7307    7460    7581 
   Size:         67     153     121

  Fragments arranged by size: 

                1090     961     954     886     662     351     283     280
                 236     231     225     217     210     153     137     131
                 121      84      69      67      67      57      54      24
                  11      11       9
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    4264    7581 
   Size:       4264    3317
 
EcoO109I rG'GnC_Cy

Cuts at:      0     301    2809    3637    7581 
   Size:        301    2508     828    3944

  Fragments arranged by size: 

                3944    2508     828     301
 
EcoRI G'AATT_C

Cuts at:      0     794    4198    6396    7581 
   Size:        794    3404    2198    1185

  Fragments arranged by size: 

                3404    2198    1185     794
 
EcoRV GAT'ATC

Cuts at:      0    2536    5798    7581 
   Size:       2536    3262    1783

  Fragments arranged by size: 

                3262    2536    1783
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1439    5877    6386    7581 
   Size:       1439    4438     509    1195

  Fragments arranged by size: 

                4438    1439    1195     509
 
HaeII r_GCGC'y

Cuts at:      0     937    1513    7581 
   Size:        937     576    6068

  Fragments arranged by size: 

                6068     937     576
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     402     434    1723    1755    2520    2552    2590    2622 
   Size:        402      32    1289      32     765      32      38      32

Cuts at:   2622    3780    3812    4148    4180    4527    4559    4662    4694 
   Size:       1158      32     336      32     347      32     103      32

Cuts at:   4694    5243    5275    6125    6157    7453    7485    7581 
   Size:        549      32     850      32    1296      32      96

  Fragments arranged by size: 

                1296    1289    1158     850     765     549     402     347
                 336     103      96      38      32      32      32      32
                  32      32      32      32      32      32      32
 
HincII GTy'rAC

Cuts at:      0     635    1268    2324    7327    7581 
   Size:        635     633    1056    5003     254

  Fragments arranged by size: 

                5003    1056     635     633     254
 
HindIII A'AGCT_T

Cuts at:      0     291     545    5234    6841    7581 
   Size:        291     254    4689    1607     740

  Fragments arranged by size: 

                4689    1607     740     291     254
 
HpaI GTT'AAC

Cuts at:      0     635    7581 
   Size:        635    6946
 
MfeI C'AATT_G

Cuts at:      0     346    4625    7581 
   Size:        346    4279    2956

  Fragments arranged by size: 

                4279    2956     346
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     294    3271    4536    5100    5603    6774    7079    7581 
   Size:        294    2977    1265     564     503    1171     305     502

  Fragments arranged by size: 

                2977    1265    1171     564     503     502     305     294
            
 
MscI TGG'CCA

Cuts at:      0    1178    2395    7581 
   Size:       1178    1217    5186

  Fragments arranged by size: 

                5186    1217    1178
 
MslI CAynn'nnrTG

Cuts at:      0     252    1071    1216    1328    3059    3310    3726    4114 
   Size:        252     819     145     112    1731     251     416     388

Cuts at:   4114    4361    6900    7066    7534    7581 
   Size:        247    2539     166     468      47

  Fragments arranged by size: 

                2539    1731     819     468     416     388     252     251
                 247     166     145     112      47
 
MspA1I CmG'CkG

Cuts at:      0      14     941    1503    3282    4563    4683    5812    6403 
   Size:         14     927     562    1779    1281     120    1129     591

Cuts at:   6403    6802    7581 
   Size:        399     779

  Fragments arranged by size: 

                1779    1281    1129     927     779     591     562     399
                 120      14
 
NcoI C'CATG_G

Cuts at:      0    6307    7581 
   Size:       6307    1274
 
NdeI CA'TA_TG

Cuts at:      0    2900    7581 
   Size:       2900    4681
 
NruI TCG'CGA

Cuts at:      0     223    7581 
   Size:        223    7358
 
NsiI A_TGCA'T

Cuts at:      0    1223    1263    1728    6463    7581 
   Size:       1223      40     465    4735    1118

  Fragments arranged by size: 

                4735    1223    1118     465      40
 
NspI r_CATG'y

Cuts at:      0     556    1112    1548    4938    5157    7581 
   Size:        556     556     436    3390     219    2424

  Fragments arranged by size: 

                3390    2424     556     556     436     219
 
PacI TTA_AT'TAA

Cuts at:      0    6332    7581 
   Size:       6332    1249
 
PciI A'CATG_T

Cuts at:      0     552    4934    7581 
   Size:        552    4382    2647

  Fragments arranged by size: 

                4382    2647     552
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     114    5340    7581 
   Size:        114    5226    2241

  Fragments arranged by size: 

                5226    2241     114
 
PfoI T'CCnGG_A

Cuts at:      0      77     113    7307    7581 
   Size:         77      36    7194     274

  Fragments arranged by size: 

                7194     274      77      36
 
PmlI CAC'GTG

Cuts at:      0    1095    7581 
   Size:       1095    6486
 
PpuMI rG'GwC_Cy

Cuts at:      0     301    3637    7581 
   Size:        301    3336    3944

  Fragments arranged by size: 

                3944    3336     301
 
PsiI TTA'TAA

Cuts at:      0     749    3995    6149    7581 
   Size:        749    3246    2154    1432

  Fragments arranged by size: 

                3246    2154    1432     749
 
PspOMI G'GGCC_C

Cuts at:      0    2809    7581 
   Size:       2809    4772
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    2294    2326    7581 
   Size:       2294      32    5255

  Fragments arranged by size: 

                5255    2294      32
 
PstI C_TGCA'G

Cuts at:      0    1781    4688    4772    7581 
   Size:       1781    2907      84    2809

  Fragments arranged by size: 

                2907    2809    1781      84
 
PvuII CAG'CTG

Cuts at:      0      14     941    1503    3282    4563    4683    5812    6403 
   Size:         14     927     562    1779    1281     120    1129     591

Cuts at:   6403    7581 
   Size:       1178

  Fragments arranged by size: 

                1779    1281    1178    1129     927     591     562     120
                  14
 
SalI G'TCGA_C

Cuts at:      0    7325    7581 
   Size:       7325     256
 
SanDI GG'GwC_CC

Cuts at:      0     301    7581 
   Size:        301    7280
 
SapI GCTCTTCn'nnn_

Cuts at:      0    4309    7581 
   Size:       4309    3272
 
ScaI AGT'ACT

Cuts at:      0    4209    6067    6891    7581 
   Size:       4209    1858     824     690

  Fragments arranged by size: 

                4209    1858     824     690
 
SexAI A'CCwGG_T

Cuts at:      0    3633    7581 
   Size:       3633    3948
 
SfcI C'TryA_G

Cuts at:      0    1777    3233    4203    4684    4768    5908    6072    7581 
   Size:       1777    1456     970     481      84    1140     164    1509

  Fragments arranged by size: 

                1777    1509    1456    1140     970     481     164      84
            
 
SmlI C'TyrA_G

Cuts at:      0    2370    3375    3445    3663    3708    5137    6356    7581 
   Size:       2370    1005      70     218      45    1429    1219    1225

  Fragments arranged by size: 

                2370    1429    1225    1219    1005     218      70      45
            
 
SpeI A'CTAG_T

Cuts at:      0    4665    7581 
   Size:       4665    2916
 
SphI G_CATG'C

Cuts at:      0    1112    1548    7581 
   Size:       1112     436    6033

  Fragments arranged by size: 

                6033    1112     436
 
SspI AAT'ATT

Cuts at:      0     753    2024    2855    4996    5601    6596    7581 
   Size:        753    1271     831    2141     605     995     985

  Fragments arranged by size: 

                2141    1271     995     985     831     753     605
 
StuI AGG'CCT

Cuts at:      0    5031    6366    7581 
   Size:       5031    1335    1215

  Fragments arranged by size: 

                5031    1335    1215
 
StyI C'CwwG_G

Cuts at:      0     333    2412    2804    4297    4409    5536    5652    5852 
   Size:        333    2079     392    1493     112    1127     116     200

Cuts at:   5852    5861    6307    6608    7581 
   Size:          9     446     301     973

  Fragments arranged by size: 

                2079    1493    1127     973     446     392     333     301
                 200     116     112       9
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    7350    7581 
   Size:       7350     231
 
TatI w'GTAC_w

Cuts at:      0    1710    4207    4784    6065    6889    7581 
   Size:       1710    2497     577    1281     824     692

  Fragments arranged by size: 

                2497    1710    1281     824     692     577
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    3060    3092    3203    3235    3409    3441    4786    4818 
   Size:       3060      32     111      32     174      32    1345      32

Cuts at:   4818    5690    5722    6000    6032    7581 
   Size:        872      32     278      32    1549

  Fragments arranged by size: 

                3060    1549    1345     872     278     174     111      32
                  32      32      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    6657    7581 
   Size:       6657     924
 
XbaI T'CTAG_A

Cuts at:      0    3733    7581 
   Size:       3733    3848
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    5108    5859    5935    7581 
   Size:       5108     751      76    1646

  Fragments arranged by size: 

                5108    1646     751      76
 
XmnI GAAnn'nnTTC

Cuts at:      0     775     794    1654    1855    2589    4875    4898    5372 
   Size:        775      19     860     201     734    2286      23     474

Cuts at:   5372    7581 
   Size:       2209

  Fragments arranged by size: 

                2286    2209     860     775     734     474     201      23
                  19
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AfeI    AflII   AflIII     AleI     AlfI
     AloI    AlwNI     ApaI    ApaLI     ApoI     AseI    AvrII    BanII
     BbsI    BbvCI    BciVI     BclI    BfrBI    BglII     BlpI Bme1580I
     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI     BsmI    BsmBI
 Bsp1286I    BspHI    BspMI    BsrDI    BsrGI    BssSI   BstAPI    BstXI
    BstYI   Bsu36I     BtgI    BtgZI     BtsI     ClaI     DraI   DraIII
     EaeI     EarI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
    HaeII    Hin4I   HincII  HindIII     HpaI     MfeI     MmeI     MscI
     MslI   MspA1I     NcoI     NdeI     NruI     NsiI     NspI     PacI
     PciI    PflMI     PfoI     PmlI    PpuMI     PsiI   PspOMI     PsrI
     PstI    PvuII     SalI    SanDI     SapI     ScaI    SexAI     SfcI
     SmlI     SpeI     SphI     SspI     StuI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AgeI     AhdI     AscI    AsiSI     AvaI
     BaeI    BamHI     BanI     BbeI     BcgI     BglI    BmgBI     BmtI
    BsaHI    BsiEI    BsiWI    BspEI    BsrBI    BsrFI   BssHII    BstBI
   BstEII  BstZ17I    CspCI     DrdI     EagI     EciI  EcoICRI     FseI
     FspI    FspAI     KasI     KpnI     MluI     NaeI     NarI   NgoMIV
     NheI     NotI     PasI     PmeI     PpiI    PshAI    PspXI     PvuI
    RsrII     SacI    SacII     SbfI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SrfI     SwaI     XhoI     XmaI     ZraI
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