Restriction map for
pF1KE5788
(Linear) (Six-base) MAPSORT of: KIEE5788.seq Check: 4678 from: 1 to: 2340
KIEE5788.orf
With 182 enzymes: SgfI *
October 24, 2019 20:50 ..
AatII G_ACGT'C
Cuts at: 0 10 1383 2340
Size: 10 1373 957
Fragments arranged by size:
1373 957 10
Acc65I G'GTAC_C
Cuts at: 0 1987 2340
Size: 1987 353
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 22 1301 1529 2340
Size: 22 1279 228 811
Fragments arranged by size:
1279 811 228 22
AflII C'TTAA_G
Cuts at: 0 1321 1855 2340
Size: 1321 534 485
Fragments arranged by size:
1321 534 485
AflIII A'CryG_T
Cuts at: 0 1238 2231 2340
Size: 1238 993 109
Fragments arranged by size:
1238 993 109
AhdI GACnn_n'nnGTC
Cuts at: 0 1379 2340
Size: 1379 961
AlwNI CAG_nnn'CTG
Cuts at: 0 988 2340
Size: 988 1352
ApoI r'AATT_y
Cuts at: 0 179 587 1586 1678 2219 2340
Size: 179 408 999 92 541 121
Fragments arranged by size:
999 541 408 179 121 92
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1309 1342 2340
Size: 1309 33 998
Fragments arranged by size:
1309 998 33
BanI G'GyrC_C
Cuts at: 0 1600 1987 2340
Size: 1600 387 353
Fragments arranged by size:
1600 387 353
BbeI G_GCGC'C
Cuts at: 0 1604 2340
Size: 1604 736
BbsI GAAGACnn'nnnn_
Cuts at: 0 53 398 2340
Size: 53 345 1942
Fragments arranged by size:
1942 345 53
BclI T'GATC_A
Cuts at: 0 851 2340
Size: 851 1489
BlpI GC'TnA_GC
Cuts at: 0 2195 2340
Size: 2195 145
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1672 2340
Size: 1672 668
Bpu10I CC'TnA_GC
Cuts at: 0 1214 2340
Size: 1214 1126
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 229 1940 2340
Size: 229 1711 400
Fragments arranged by size:
1711 400 229
BsaHI Gr'CG_yC
Cuts at: 0 7 1380 1601 2340
Size: 7 1373 221 739
Fragments arranged by size:
1373 739 221 7
BsaWI w'CCGG_w
Cuts at: 0 1665 2340
Size: 1665 675
BseYI C'CCAG_C
Cuts at: 0 171 2340
Size: 171 2169
BsiHKAI G_wGCw'C
Cuts at: 0 735 1313 2288 2340
Size: 735 578 975 52
Fragments arranged by size:
975 735 578 52
BsmI GAATG_Cn'
Cuts at: 0 793 1599 2340
Size: 793 806 741
Fragments arranged by size:
806 793 741
Bsp1286I G_dGCh'C
Cuts at: 0 735 1313 2288 2340
Size: 735 578 975 52
Fragments arranged by size:
975 735 578 52
BsrDI GCAATG_nn'
Cuts at: 0 482 2340
Size: 482 1858
BsrGI T'GTAC_A
Cuts at: 0 678 1329 2340
Size: 678 651 1011
Fragments arranged by size:
1011 678 651
BssSI C'ACGA_G
Cuts at: 0 233 2340
Size: 233 2107
BstBI TT'CG_AA
Cuts at: 0 405 2340
Size: 405 1935
BstEII G'GTnAC_C
Cuts at: 0 1782 2340
Size: 1782 558
Bsu36I CC'TnA_GG
Cuts at: 0 572 2340
Size: 572 1768
BtsI GCAGTG_nn'
Cuts at: 0 121 2340
Size: 121 2219
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2254 2289 2340
Size: 2254 35 51
Fragments arranged by size:
2254 51 35
DraI TTT'AAA
Cuts at: 0 194 819 1149 2340
Size: 194 625 330 1191
Fragments arranged by size:
1191 625 330 194
DraIII CAC_nnn'GTG
Cuts at: 0 310 2340
Size: 310 2030
DrdI GACnn_nn'nnGTC
Cuts at: 0 1902 2340
Size: 1902 438
EarI CTCTTCn'nnn_
Cuts at: 0 41 1277 1999 2099 2295 2340
Size: 41 1236 722 100 196 45
Fragments arranged by size:
1236 722 196 100 45 41
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 22 1301 1529 1672 2340
Size: 22 1279 228 143 668
Fragments arranged by size:
1279 668 228 143 22
EcoRI G'AATT_C
Cuts at: 0 1678 2340
Size: 1678 662
EcoRV GAT'ATC
Cuts at: 0 2316 2340
Size: 2316 24
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 52 223 633 1315 2340
Size: 52 171 410 682 1025
Fragments arranged by size:
1025 682 410 171 52
HaeII r_GCGC'y
Cuts at: 0 1604 2340
Size: 1604 736
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 29 1365 1397 1823 1855 2340
Size: 29 1336 32 426 32 485
Fragments arranged by size:
1336 485 426 32 32 29
HincII GTy'rAC
Cuts at: 0 337 555 1962 2340
Size: 337 218 1407 378
Fragments arranged by size:
1407 378 337 218
HindIII A'AGCT_T
Cuts at: 0 82 409 509 1070 1297 1474 1523 1858
Size: 82 327 100 561 227 177 49 335
Cuts at: 1858 2340
Size: 482
Fragments arranged by size:
561 482 335 327 227 177 100 82
49
HpaI GTT'AAC
Cuts at: 0 555 2340
Size: 555 1785
KasI G'GCGC_C
Cuts at: 0 1600 2340
Size: 1600 740
KpnI G_GTAC'C
Cuts at: 0 1991 2340
Size: 1991 349
MslI CAynn'nnrTG
Cuts at: 0 251 2332 2340
Size: 251 2081 8
Fragments arranged by size:
2081 251 8
MspA1I CmG'CkG
Cuts at: 0 525 985 1459 1567 2199 2340
Size: 525 460 474 108 632 141
Fragments arranged by size:
632 525 474 460 141 108
NarI GG'CG_CC
Cuts at: 0 1601 2340
Size: 1601 739
NspI r_CATG'y
Cuts at: 0 145 493 1242 2235 2340
Size: 145 348 749 993 105
Fragments arranged by size:
993 749 348 145 105
PciI A'CATG_T
Cuts at: 0 1238 2231 2340
Size: 1238 993 109
Fragments arranged by size:
1238 993 109
PsiI TTA'TAA
Cuts at: 0 1744 2340
Size: 1744 596
PstI C_TGCA'G
Cuts at: 0 2204 2340
Size: 2204 136
PvuII CAG'CTG
Cuts at: 0 525 985 1567 2199 2340
Size: 525 460 582 632 141
Fragments arranged by size:
632 582 525 460 141
ScaI AGT'ACT
Cuts at: 0 239 2340
Size: 239 2101
SfcI C'TryA_G
Cuts at: 0 2084 2200 2340
Size: 2084 116 140
Fragments arranged by size:
2084 140 116
SfoI GGC'GCC
Cuts at: 0 1602 2340
Size: 1602 738
SmlI C'TyrA_G
Cuts at: 0 208 1321 1855 1955 2340
Size: 208 1113 534 100 385
Fragments arranged by size:
1113 534 385 208 100
SphI G_CATG'C
Cuts at: 0 145 2340
Size: 145 2195
StyI C'CwwG_G
Cuts at: 0 843 2340
Size: 843 1497
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1983 2340
Size: 1983 357
TatI w'GTAC_w
Cuts at: 0 237 678 1329 2340
Size: 237 441 651 1011
Fragments arranged by size:
1011 651 441 237
XmnI GAAnn'nnTTC
Cuts at: 0 203 1073 2340
Size: 203 870 1267
Fragments arranged by size:
1267 870 203
ZraI GAC'GTC
Cuts at: 0 8 1381 2340
Size: 8 1373 959
Fragments arranged by size:
1373 959 8
Enzymes that do cut:
AatII Acc65I AcuI AflII AflIII AhdI AlwNI ApoI
BaeI BanI BbeI BbsI BclI BlpI BpmI Bpu10I
BpuEI BsaHI BsaWI BseYI BsiHKAI BsmI Bsp1286I BsrDI
BsrGI BssSI BstBI BstEII Bsu36I BtsI CspCI DraI
DraIII DrdI EarI Eco57MI EcoRI EcoRV FalI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MslI MspA1I
NarI NspI PciI PsiI PstI PvuII ScaI SfcI
SfoI SmlI SphI StyI TaqII TatI XmnI ZraI
Enzymes that do not cut:
AarI AccI AclI AfeI AgeI AleI AlfI AloI
ApaI ApaLI AscI AseI AsiSI AvaI AvrII BamHI
BanII BbvCI BcgI BciVI BfrBI BglI BglII Bme1580I
BmgBI BmrI BmtI BplI BsaI BsaAI BsaBI BsaXI
BseRI BsgI BsiEI BsiWI BsmBI BspEI BspHI BspMI
BsrBI BsrFI BssHII BstAPI BstXI BstYI BstZ17I BtgI
BtgZI ClaI EaeI EagI EciI EcoICRI EcoNI EcoO109I
FseI FspI FspAI MfeI MluI MmeI MscI NaeI
NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI SexAI SfiI SgfI SgrAI SmaI
SnaBI SpeI SrfI SspI StuI SwaI TstI Tth111I
XbaI XcmI XhoI XmaI