Restriction map for
pF1KE9424
(Circular) (Six-base) MAPSORT of: pF1KE9424.seq Check: 7577 from: 1 to: 4196
pF1KE9424 4196 bp
With 182 enzymes: SgfI *
November 2, 2010 17:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 452 542 452
Size: 90 4106
AatII G_ACGT'C
Cuts at: 200 200
Size: 4196
AccI GT'mk_AC
Cuts at: 1234 1234
Size: 4196
Acc65I G'GTAC_C
Cuts at: 1212 1212
Size: 4196
AclI AA'CG_TT
Cuts at: 4036 4036
Size: 4196
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2021 2453 3200 3468 2021
Size: 432 747 268 2749
Fragments arranged by size:
2749 747 432 268
AfeI AGC'GCT
Cuts at: 378 378
Size: 4196
AflIII A'CryG_T
Cuts at: 281 1548 2652 3629 281
Size: 1267 1104 977 848
Fragments arranged by size:
1267 1104 977 848
AgeI A'CCGG_T
Cuts at: 2584 2584
Size: 4196
AlwNI CAG_nnn'CTG
Cuts at: 1501 3068 1501
Size: 1567 2629
ApaI G_GGCC'C
Cuts at: 430 430
Size: 4196
ApaLI G'TGCA_C
Cuts at: 529 2966 529
Size: 2437 1759
ApoI r'AATT_y
Cuts at: 677 794 1103 1200 3549 3805 677
Size: 117 309 97 2349 256 1068
Fragments arranged by size:
2349 1068 309 256 117 97
AscI GG'CGCG_CC
Cuts at: 304 304
Size: 4196
AseI AT'TA_AT
Cuts at: 20 20
Size: 4196
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4196
AvaI C'yCGr_G
Cuts at: 1216 1216
Size: 4196
BamHI G'GATC_C
Cuts at: 1221 1221
Size: 4196
BanI G'GyrC_C
Cuts at: 664 759 1212 1626 1874 1909 664
Size: 95 453 414 248 35 2951
Fragments arranged by size:
2951 453 414 248 95 35
BanII G_rGCy'C
Cuts at: 430 943 1210 2240 430
Size: 513 267 1030 2386
Fragments arranged by size:
2386 1030 513 267
BbeI G_GCGC'C
Cuts at: 668 1630 1878 668
Size: 962 248 2986
Fragments arranged by size:
2986 962 248
BbsI GAAGACnn'nnnn_
Cuts at: 624 624
Size: 4196
BciVI GTATCCnnnnn_n'
Cuts at: 2094 2855 4196 2094
Size: 761 1341 2094
Fragments arranged by size:
2094 1341 761
BclI T'GATC_A
Cuts at: 779 1716 779
Size: 937 3259
BglII A'GATC_T
Cuts at: 719 1711 3748 719
Size: 992 2037 1167
Fragments arranged by size:
2037 1167 992
BlpI GC'TnA_GC
Cuts at: 1308 1308
Size: 4196
Bme1580I G_kGCm'C
Cuts at: 430 533 1821 1914 2970 430
Size: 103 1288 93 1056 1656
Fragments arranged by size:
1656 1288 1056 103 93
BmrI ACTGGGnnnn_n'
Cuts at: 1408 1489 1824 3968 1408
Size: 81 335 2144 1636
Fragments arranged by size:
2144 1636 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2563 2563
Size: 4196
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 178 529 734 178
Size: 351 205 3640
Fragments arranged by size:
3640 351 205
Bpu10I CC'TnA_GC
Cuts at: 466 466
Size: 4196
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1372 2251 2743 3041 1372
Size: 879 492 298 2527
Fragments arranged by size:
2527 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 924 3900 924
Size: 2976 1220
BsaAI yAC'GTr
Cuts at: 456 1549 456
Size: 1093 3103
BsaBI GATnn'nnATC
Cuts at: 6 1710 3747 6
Size: 1704 2037 455
Fragments arranged by size:
2037 1704 455
BsaHI Gr'CG_yC
Cuts at: 197 315 665 1627 1875 2577 4073 197
Size: 118 350 962 248 702 1496 320
Fragments arranged by size:
1496 962 702 350 320 248 118
BsaWI w'CCGG_w
Cuts at: 1385 1906 2584 2858 3005 3416 1385
Size: 521 678 274 147 411 2165
Fragments arranged by size:
2165 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 901 952 901
Size: 51 4145
BseYI C'CCAG_C
Cuts at: 273 423 875 1621 2379 2956 3452 273
Size: 150 452 746 758 577 496 1017
Fragments arranged by size:
1017 758 746 577 496 452 150
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1104 1104
Size: 4196
BsiEI CG_ry'CG
Cuts at: 104 611 1738 1784 2992 104
Size: 507 1127 46 1208 1308
Fragments arranged by size:
1308 1208 1127 507 46
BsiHKAI G_wGCw'C
Cuts at: 254 533 1210 1988 2178 2970 254
Size: 279 677 778 190 792 1480
Fragments arranged by size:
1480 792 778 677 279 190
BsmI GAATG_Cn'
Cuts at: 124 124
Size: 4196
Bsp1286I G_dGCh'C
Cuts at: 254 430 533 943 1210 1821 1914 1988 2178
Size: 176 103 410 267 611 93 74 190
Cuts at: 2178 2240 2970 254
Size: 62 730 1480
Fragments arranged by size:
1480 730 611 410 267 190 176 103
93 74 62
BspEI T'CCGG_A
Cuts at: 3416 3416
Size: 4196
BspHI T'CATG_A
Cuts at: 3372 3372
Size: 4196
BspMI ACCTGCnnnn'nnnn_
Cuts at: 371 452 542 1246 1460 1762 2143 371
Size: 81 90 704 214 302 381 2424
Fragments arranged by size:
2424 704 381 302 214 90 81
BsrBI CCG'CTC
Cuts at: 50 470 616 2488 2542 4026 4191 50
Size: 420 146 1872 54 1484 165 55
Fragments arranged by size:
1872 1484 420 165 146 55 54
BsrDI GCAATG_nn'
Cuts at: 2108 2108
Size: 4196
BsrFI r'CCGG_y
Cuts at: 212 2194 2375 2584 212
Size: 1982 181 209 1824
Fragments arranged by size:
1982 1824 209 181
BssHII G'CGCG_C
Cuts at: 122 304 122
Size: 182 4014
BssSI C'ACGA_G
Cuts at: 723 2467 2825 723
Size: 1744 358 2094
Fragments arranged by size:
2094 1744 358
BstAPI GCAn_nnn'nTGC
Cuts at: 284 521 1573 284
Size: 237 1052 2907
Fragments arranged by size:
2907 1052 237
BstBI TT'CG_AA
Cuts at: 2557 2557
Size: 4196
BstYI r'GATC_y
Cuts at: 1 719 863 1221 1688 1711 2046 2292 3304
Size: 718 144 358 467 23 335 246 1012
Cuts at: 3304 3390 3402 3748 1
Size: 86 12 346 449
Fragments arranged by size:
1012 718 467 449 358 346 335 246
144 86 23 12
BtgI C'CryG_G
Cuts at: 227 318 497 227
Size: 91 179 3926
Fragments arranged by size:
3926 179 91
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2146 2327 2490 2146
Size: 181 163 3852
Fragments arranged by size:
3852 181 163
BtsI GCAGTG_nn'
Cuts at: 269 581 1439 269
Size: 312 858 3026
Fragments arranged by size:
3026 858 312
DraI TTT'AAA
Cuts at: 1195 1195
Size: 4196
DrdI GACnn_nn'nnGTC
Cuts at: 1156 1902 2760 1156
Size: 746 858 2592
Fragments arranged by size:
2592 858 746
EaeI y'GGCC_r
Cuts at: 224 395 494 1003 1781 1955 2346 2373 3718
Size: 171 99 509 778 174 391 27 1345
Cuts at: 3718 224
Size: 702
Fragments arranged by size:
1345 778 702 509 391 174 171 99
27
EagI C'GGCC_G
Cuts at: 1781 1781
Size: 4196
EarI CTCTTCn'nnn_
Cuts at: 355 373 454 817 898 910 1018 2219 2429
Size: 18 81 363 81 12 108 1201 210
Cuts at: 2429 355
Size: 2122
Fragments arranged by size:
2122 1201 363 210 108 81 81 18
12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2404 2714 2860 4006 2404
Size: 310 146 1146 2594
Fragments arranged by size:
2594 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1208 1208
Size: 4196
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 178 529 734 2021 2453 3200 3468 178
Size: 351 205 1287 432 747 268 906
Fragments arranged by size:
1287 906 747 432 351 268 205
EcoO109I rG'GnC_Cy
Cuts at: 427 553 1335 427
Size: 126 782 3288
Fragments arranged by size:
3288 782 126
EcoRI G'AATT_C
Cuts at: 1200 1200
Size: 4196
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2215 2215
Size: 4196
FspI TGC'GCA
Cuts at: 151 1977 151
Size: 1826 2370
HaeII r_GCGC'y
Cuts at: 380 589 668 1630 1878 2900 3879 380
Size: 209 79 962 248 1022 979 697
Fragments arranged by size:
1022 979 962 697 248 209 79
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2038 2068 2070 2100 2038
Size: 30 2 30 4134
Fragments arranged by size:
4134 30 30 2
HincII GTy'rAC
Cuts at: 1235 1235
Size: 4196
KasI G'GCGC_C
Cuts at: 664 1626 1874 664
Size: 962 248 2986
Fragments arranged by size:
2986 962 248
KpnI G_GTAC'C
Cuts at: 1216 1216
Size: 4196
MluI A'CGCG_T
Cuts at: 3629 3629
Size: 4196
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 218 2867 3051 218
Size: 2649 184 1363
Fragments arranged by size:
2649 1363 184
MscI TGG'CCA
Cuts at: 1957 1957
Size: 4196
MslI CAynn'nnrTG
Cuts at: 2185 2312 2185
Size: 127 4069
MspA1I CmG'CkG
Cuts at: 133 229 320 499 1307 1621 1981 2994 3239
Size: 96 91 179 808 314 360 1013 245
Cuts at: 3239 133
Size: 1090
Fragments arranged by size:
1090 1013 808 360 314 245 179 96
91
NaeI GCC'GGC
Cuts at: 2377 2377
Size: 4196
NarI GG'CG_CC
Cuts at: 665 1627 1875 665
Size: 962 248 2986
Fragments arranged by size:
2986 962 248
NgoMIV G'CCGG_C
Cuts at: 2375 2375
Size: 4196
NspI r_CATG'y
Cuts at: 1249 1749 2656 1249
Size: 500 907 2789
Fragments arranged by size:
2789 907 500
PciI A'CATG_T
Cuts at: 2652 2652
Size: 4196
PflMI CCAn_nnn'nTGG
Cuts at: 216 216
Size: 4196
PfoI T'CCnGG_A
Cuts at: 711 711
Size: 4196
PmeI GTTT'AAAC
Cuts at: 1195 1195
Size: 4196
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3335 3367 3335
Size: 32 4164
PpuMI rG'GwC_Cy
Cuts at: 553 553
Size: 4196
PspOMI G'GGCC_C
Cuts at: 426 426
Size: 4196
PstI C_TGCA'G
Cuts at: 539 1243 1928 539
Size: 704 685 2807
Fragments arranged by size:
2807 704 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4196
PvuII CAG'CTG
Cuts at: 1621 1981 1621
Size: 360 3836
RsrII CG'GwC_CG
Cuts at: 2391 2391
Size: 4196
SacI G_AGCT'C
Cuts at: 1210 1210
Size: 4196
SacII CC_GC'GG
Cuts at: 230 321 500 230
Size: 91 179 3926
Fragments arranged by size:
3926 179 91
SalI G'TCGA_C
Cuts at: 1233 1233
Size: 4196
SapI GCTCTTCn'nnn_
Cuts at: 373 454 898 2219 2429 373
Size: 81 444 1321 210 2140
Fragments arranged by size:
2140 1321 444 210 81
SbfI CC_TGCA'GG
Cuts at: 1243 1243
Size: 4196
ScaI AGT'ACT
Cuts at: 3713 3713
Size: 4196
SexAI A'CCwGG_T
Cuts at: 992 992
Size: 4196
SfcI C'TryA_G
Cuts at: 33 535 1239 1924 2917 3108 33
Size: 502 704 685 993 191 1121
Fragments arranged by size:
1121 993 704 685 502 191
SfoI GGC'GCC
Cuts at: 666 1628 1876 666
Size: 962 248 2986
Fragments arranged by size:
2986 962 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4196
SmaI CCC'GGG
Cuts at: 1218 1218
Size: 4196
SmlI C'TyrA_G
Cuts at: 1351 2266 2758 3020 1351
Size: 915 492 262 2527
Fragments arranged by size:
2527 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4196
SphI G_CATG'C
Cuts at: 1249 1749 1249
Size: 500 3696
StuI AGG'CCT
Cuts at: 415 415
Size: 4196
StyI C'CwwG_G
Cuts at: 1330 1684 1330
Size: 354 3842
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1011 1779 1912 2371 2580 1011
Size: 768 133 459 209 2627
Fragments arranged by size:
2627 768 459 209 133
TatI w'GTAC_w
Cuts at: 3711 3711
Size: 4196
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 165 197 3713 3745 165
Size: 32 3516 32 616
Fragments arranged by size:
3516 616 32 32
Tth111I GACn'n_nGTC
Cuts at: 994 1993 994
Size: 999 3197
XbaI T'CTAG_A
Cuts at: 1227 1227
Size: 4196
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 489 1691 489
Size: 1202 2994
XmaI C'CCGG_G
Cuts at: 1216 1216
Size: 4196
ZraI GAC'GTC
Cuts at: 198 198
Size: 4196
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BciVI BclI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PciI PflMI PfoI PmeI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AflII AhdI AleI AlfI AloI AvrII BaeI BbvCI
BcgI BfrBI BglI BmgBI BmtI BsaXI BsiWI BsmBI
BsrGI BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PmlI
PshAI PsiI PspXI PsrI SanDI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI XmnI