Restriction map for pF1KE9424
 (Linear) (Six-base) MAPSORT of: KIEE9424.seq  Check: 1110  from: 1  to: 1083



KIEE9424.orf

 With 182 enzymes: SgfI * 

                        November 2, 2010 17:11  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     344     434    1083 
   Size:        344      90     649

  Fragments arranged by size: 

                 649     344      90
 
AatII G_ACGT'C

Cuts at:      0      92    1083 
   Size:         92     991
 
AfeI AGC'GCT

Cuts at:      0     270    1083 
   Size:        270     813
 
AflIII A'CryG_T

Cuts at:      0     173    1083 
   Size:        173     910
 
ApaI G_GGCC'C

Cuts at:      0     322    1083 
   Size:        322     761
 
ApaLI G'TGCA_C

Cuts at:      0     421    1083 
   Size:        421     662
 
ApoI r'AATT_y

Cuts at:      0     569     686     995    1083 
   Size:        569     117     309      88

  Fragments arranged by size: 

                 569     309     117      88
 
AscI GG'CGCG_CC

Cuts at:      0     196    1083 
   Size:        196     887
 
BanI G'GyrC_C

Cuts at:      0     556     651    1083 
   Size:        556      95     432

  Fragments arranged by size: 

                 556     432      95
 
BanII G_rGCy'C

Cuts at:      0     322     835    1083 
   Size:        322     513     248

  Fragments arranged by size: 

                 513     322     248
 
BbeI G_GCGC'C

Cuts at:      0     560    1083 
   Size:        560     523
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     516    1083 
   Size:        516     567
 
BclI T'GATC_A

Cuts at:      0     671    1083 
   Size:        671     412
 
BglII A'GATC_T

Cuts at:      0     611    1083 
   Size:        611     472
 
Bme1580I G_kGCm'C

Cuts at:      0     322     425    1083 
   Size:        322     103     658

  Fragments arranged by size: 

                 658     322     103
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      70     421     626    1083 
   Size:         70     351     205     457

  Fragments arranged by size: 

                 457     351     205      70
 
Bpu10I CC'TnA_GC

Cuts at:      0     358    1083 
   Size:        358     725
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     816    1083 
   Size:        816     267
 
BsaAI yAC'GTr

Cuts at:      0     348    1083 
   Size:        348     735
 
BsaHI Gr'CG_yC

Cuts at:      0      89     207     557    1083 
   Size:         89     118     350     526

  Fragments arranged by size: 

                 526     350     118      89
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     793     844    1083 
   Size:        793      51     239

  Fragments arranged by size: 

                 793     239      51
 
BseYI C'CCAG_C

Cuts at:      0     165     315     767    1083 
   Size:        165     150     452     316

  Fragments arranged by size: 

                 452     316     165     150
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     996    1083 
   Size:        996      87
 
BsiEI CG_ry'CG

Cuts at:      0     503    1083 
   Size:        503     580
 
BsiHKAI G_wGCw'C

Cuts at:      0     146     425    1083 
   Size:        146     279     658

  Fragments arranged by size: 

                 658     279     146
 
BsmI GAATG_Cn'

Cuts at:      0      16    1083 
   Size:         16    1067
 
Bsp1286I G_dGCh'C

Cuts at:      0     146     322     425     835    1083 
   Size:        146     176     103     410     248

  Fragments arranged by size: 

                 410     248     176     146     103
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     263     344     434    1083 
   Size:        263      81      90     649

  Fragments arranged by size: 

                 649     263      90      81
 
BsrBI CCG'CTC

Cuts at:      0     362     508    1083 
   Size:        362     146     575

  Fragments arranged by size: 

                 575     362     146
 
BsrFI r'CCGG_y

Cuts at:      0     104    1083 
   Size:        104     979
 
BssHII G'CGCG_C

Cuts at:      0      14     196    1083 
   Size:         14     182     887

  Fragments arranged by size: 

                 887     182      14
 
BssSI C'ACGA_G

Cuts at:      0     615    1083 
   Size:        615     468
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     176     413    1083 
   Size:        176     237     670

  Fragments arranged by size: 

                 670     237     176
 
BstYI r'GATC_y

Cuts at:      0     611     755    1083 
   Size:        611     144     328

  Fragments arranged by size: 

                 611     328     144
 
BtgI C'CryG_G

Cuts at:      0     119     210     389    1083 
   Size:        119      91     179     694

  Fragments arranged by size: 

                 694     179     119      91
 
BtsI GCAGTG_nn'

Cuts at:      0     161     473    1083 
   Size:        161     312     610

  Fragments arranged by size: 

                 610     312     161
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1048    1083 
   Size:       1048      35
 
EaeI y'GGCC_r

Cuts at:      0     116     287     386     895    1083 
   Size:        116     171      99     509     188

  Fragments arranged by size: 

                 509     188     171     116      99
 
EarI CTCTTCn'nnn_

Cuts at:      0     247     265     346     709     790     802     910    1083 
   Size:        247      18      81     363      81      12     108     173

  Fragments arranged by size: 

                 363     247     173     108      81      81      18      12
            
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      70     421     626    1083 
   Size:         70     351     205     457

  Fragments arranged by size: 

                 457     351     205      70
 
EcoO109I rG'GnC_Cy

Cuts at:      0     319     445    1083 
   Size:        319     126     638

  Fragments arranged by size: 

                 638     319     126
 
FspI TGC'GCA

Cuts at:      0      43    1083 
   Size:         43    1040
 
HaeII r_GCGC'y

Cuts at:      0     272     481     560    1083 
   Size:        272     209      79     523

  Fragments arranged by size: 

                 523     272     209      79
 
KasI G'GCGC_C

Cuts at:      0     556    1083 
   Size:        556     527
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     110    1083 
   Size:        110     973
 
MspA1I CmG'CkG

Cuts at:      0      25     121     212     391    1083 
   Size:         25      96      91     179     692

  Fragments arranged by size: 

                 692     179      96      91      25
 
NarI GG'CG_CC

Cuts at:      0     557    1083 
   Size:        557     526
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     108    1083 
   Size:        108     975
 
PfoI T'CCnGG_A

Cuts at:      0     603    1083 
   Size:        603     480
 
PpuMI rG'GwC_Cy

Cuts at:      0     445    1083 
   Size:        445     638
 
PspOMI G'GGCC_C

Cuts at:      0     318    1083 
   Size:        318     765
 
PstI C_TGCA'G

Cuts at:      0     431    1083 
   Size:        431     652
 
SacII CC_GC'GG

Cuts at:      0     122     213     392    1083 
   Size:        122      91     179     691

  Fragments arranged by size: 

                 691     179     122      91
 
SapI GCTCTTCn'nnn_

Cuts at:      0     265     346     790    1083 
   Size:        265      81     444     293

  Fragments arranged by size: 

                 444     293     265      81
 
SexAI A'CCwGG_T

Cuts at:      0     884    1083 
   Size:        884     199
 
SfcI C'TryA_G

Cuts at:      0     427    1083 
   Size:        427     656
 
SfoI GGC'GCC

Cuts at:      0     558    1083 
   Size:        558     525
 
StuI AGG'CCT

Cuts at:      0     307    1083 
   Size:        307     776
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     903    1083 
   Size:        903     180
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      57      89    1083 
   Size:         57      32     994

  Fragments arranged by size: 

                 994      57      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     886    1083 
   Size:        886     197
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     381    1083 
   Size:        381     702
 
ZraI GAC'GTC

Cuts at:      0      90    1083 
   Size:         90     993
 

 Enzymes that do cut:

     AarI    AatII     AfeI   AflIII     ApaI    ApaLI     ApoI     AscI
     BanI    BanII     BbeI     BbsI     BclI    BglII Bme1580I     BpmI
   Bpu10I     BsaI    BsaAI    BsaHI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI     BsmI Bsp1286I    BspMI    BsrBI    BsrFI   BssHII    BssSI
   BstAPI    BstYI     BtgI     BtsI     DrdI     EaeI     EarI  Eco57MI
 EcoO109I     FspI    HaeII     KasI     MmeI   MspA1I     NarI    PflMI
     PfoI    PpuMI   PspOMI     PstI    SacII     SapI    SexAI     SfcI
     SfoI     StuI    TaqII     TstI  Tth111I     XcmI     ZraI

 Enzymes that do not cut: 

     AccI   Acc65I     AclI     AcuI    AflII     AgeI     AhdI     AleI
     AlfI     AloI    AlwNI     AseI    AsiSI     AvaI    AvrII     BaeI
    BamHI    BbvCI     BcgI    BciVI    BfrBI     BglI     BlpI    BmgBI
     BmrI     BmtI     BplI    BpuEI    BsaBI    BsaWI    BsaXI    BsiWI
    BsmBI    BspEI    BspHI    BsrDI    BsrGI    BstBI   BstEII    BstXI
  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII     EagI
     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI    FspAI
    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MscI
     MslI     NaeI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI     PmeI     PmlI     PpiI
    PshAI     PsiI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI
     SalI    SanDI     SbfI     ScaI     SfiI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StyI     SwaI
     TatI     XbaI     XhoI     XmaI     XmnI
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