Restriction map for pF1KE3818
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# NO CUT ENZYMES (   83 enzymes) #
##################################

AccIII      AclI        AgeI        Aor51HI     ApaI        
AscI        AseI        AsnI        AviII       AvrII       
BbeI        BbrPI       BbsI        BciVI       BlnI        
BpuAI       BsaAI       BsaBI       BsaWI       BseAI       
BsiWI       BsmBI       BspDI       BspEI       BspLU11I    
BsrBI       BssHII      BssSI       Bst1107I    BstBI       
BstEII      BstPI       BstZ17I     ClaI        CpoI        
DraI        DrdI        Eco47III    EcoO65I     EcoT22I     
FseI        FspI        HaeII       HincII      HindII      
HpaI        KasI        MamI        MfeI        MluI        
MroI        MunI        NaeI        NarI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PinAI       PmaCI       
PmeI        PmlI        PshBI       Psp1406I    PvuI        
RsrII       SalI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SnaBI       SpeI        SspI        
SwaI        XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AatII        1399  GACGTC               GACGT^C
Acc65I        518  GGTACC               G^GTACC
AccI         3328  GTAGAC               GT^[AC][GT]AC
AflII        1287  CTTAAG               C^TTAAG
AflIII        645  ACGTGT               A^C[GA][CT]GT
AhdI         3280  GACCTACTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I       518  GGTACC               G^GTACC
AspEI        3280  GACCTACTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         2442  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI         3478  CCCGGG               C^[CT]CG[GA]G
BcgI         2129  CGAATCTGGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI          156  TGATCA               T^GATCA
BfrI         1287  CTTAAG               C^TTAAG
BglII        1224  AGATCT               A^GATCT
BlpI          558  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      558  GCTCAGC              GC^T[ACGT]AGC
BsiEI          93  CGGCCG               CG[GA][CT]^CG
BsmI         1123  GAATGC               GAATGC(1/-1)
BsoBI        3478  CCCGGG               C^[CT]CG[GA]G
BspHI        1967  TCATGA               T^CATGA
BsrFI         130  ACCGGC               [GA]^CCGG[CT]
BstAPI       1783  GCACAAGATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI         167  CCAGCTGTGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII         558  GCTCAGC              GC^T[ACGT]AGC
Cfr10I        130  ACCGGC               [GA]^CCGG[CT]
EagI           93  CGGCCG               C^GGCCG
Eam1105I     3280  GACCTACTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI          93  CGGCCG               C^GGCCG
Eco52I         93  CGGCCG               C^GGCCG
EcoNI        1470  CCTGTCTCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI         982  GAATTC               G^AATTC
EcoRV        1502  GATATC               GAT^ATC
FbaI          156  TGATCA               T^GATCA
KpnI          518  GGTACC               GGTAC^C
KspI           57  CCGCGG               CCGC^GG
PflFI        2442  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        2713  CCAGAGGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI         527  GACCTGTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI         1967  TCATGA               T^CATGA
SacI          285  GAGCTC               GAGCT^C
SacII          57  CCGCGG               CCGC^GG
SbfI         3438  CCTGCAGG             CCTGCA^GG
ScaI         1193  AGTACT               AGT^ACT
SmaI         3478  CCCGGG               CCC^GGG
Sse8387I     3438  CCTGCAGG             CCTGCA^GG
StuI         1844  AGGCCT               AGG^CCT
Tth111I      2442  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I       2713  CCAGAGGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI        3478  CCCGGG               C^CCGGG
XmaI         3478  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   35 enzymes) #
###################################

AccII          19  CGCG                 CG^CG
               58  CGCG                
AcyI          913  GGCGTC               G[GA]^CG[CT]C
             1399  GACGTC              
Alw44I       1838  GTGCAC               G^TGCAC
             3293  GTGCAC              
ApaLI        1838  GTGCAC               G^TGCAC
             3293  GTGCAC              
Asp700I      1206  GAAGAATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2865  GAATAAATTC          
BalI         1166  TGGCCA               TGG^CCA
             3173  TGGCCA              
BamHI          62  GGATCC               G^GATCC
             1855  GGATCC              
BbvCI          49  CCTCAGC              CCTCAGC(-5/-2)
             1657  GCTGAGG             
BglI         2730  GCCGGGCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             3166  GCCAAAATGGC         
BsaHI         913  GGCGTC               G[GA]^CG[CT]C
             1399  GACGTC              
BsaI          127  GAGACC               GGTCTC(1/5)
             2538  GAGACC              
Bsp1407I      732  TGTACA               T^GTACA
             3516  TGTACA              
BsrDI        1186  GCAATG               GCAATG(2/0)
             2392  GCAATG              
BsrGI         732  TGTACA               T^GTACA
             3516  TGTACA              
BstUI          19  CGCG                 CG^CG
               58  CGCG                
CfoI          117  GCGC                 GCG^C
             3083  GCGC                
DraIII        536  CACGCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
             2785  CACAAAGTG           
HgaI           20  GCGTC                GACGC(5/10)
              914  GCGTC               
HhaI          117  GCGC                 GCG^C
             3083  GCGC                
Hin1I         913  GGCGTC               G[GA]^CG[CT]C
             1399  GACGTC              
HinP1I        117  GCGC                 G^CGC
             3083  GCGC                
HindIII       221  AAGCTT               A^AGCTT
             1610  AAGCTT              
MluNI        1166  TGGCCA               TGG^CCA
             3173  TGGCCA              
MscI         1166  TGGCCA               TGG^CCA
             3173  TGGCCA              
MvnI           19  CGCG                 CG^CG
               58  CGCG                
NcoI          522  CCATGG               C^CATGG
              968  CCATGG              
PpuMI         104  GGGACCC              [GA]G^G[AT]CC[CT]
             2621  AGGACCC             
SapI         3010  GAAGAGC              GCTCTTC(1/4)
             2575  GCTCTTC             
SmlI         1287  CTTAAG               C^T[CT][GA]AG
             3131  CTCAAG              
SphI         1558  GCATGC               GCATG^C
             2088  GCATGC              
SspBI         732  TGTACA               T^GTACA
             3516  TGTACA              
TaqI         1506  TCGA                 T^CGA
             1872  TCGA                
TfiI         2028  GAATC                G^A[AT]TC
             2130  GAATC               
TthHB8I      1506  TCGA                 T^CGA
             1872  TCGA                
XmnI         1206  GAAGAATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2865  GAATAAATTC          


#########################################
# 3 OR MORE CUT ENZYMES (   90 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       AocI        ApoI        AspHI       AvaII       
BanI        BanII       BbvI        BcnI        BfaI        
BmyI        BpmI        BsaJI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    BspMI       BsrI        BssKI       BstF5I      
BstNI       BstYI       Bsu36I      Cac8I       Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EarI        Eco81I      EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeIII      HapII       
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MnlI        MseI        MslI        MspA1I      
MspI        MvaI        MwoI        NciI        NdeII       
NlaIII      NlaIV       NspI        PleI        PstI        
PvuII       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        StyI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       XcmI        XhoII       
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