Restriction map for pF1KE3818
 (Linear) (Six-base) MAPSORT of: KIEE3818.seq  Check: 3396  from: 1  to: 3546



KIEE3818.orf

 With 182 enzymes: SgfI * 

                        October 24, 2019 20:50  ..

AatII G_ACGT'C

Cuts at:      0    1403    3546 
   Size:       1403    2143
 
AccI GT'mk_AC

Cuts at:      0    3329    3546 
   Size:       3329     217
 
Acc65I G'GTAC_C

Cuts at:      0     518    3546 
   Size:        518    3028
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     696    1667    1765    1941    3443    3546 
   Size:        696     971      98     176    1502     103

  Fragments arranged by size: 

                1502     971     696     176     103      98
 
AflII C'TTAA_G

Cuts at:      0    1287    3546 
   Size:       1287    2259
 
AflIII A'CryG_T

Cuts at:      0     645    3546 
   Size:        645    2901
 
AhdI GACnn_n'nnGTC

Cuts at:      0    3285    3546 
   Size:       3285     261
 
AleI CACnn'nnGTG

Cuts at:      0     490     792    2145    3546 
   Size:        490     302    1353    1401

  Fragments arranged by size: 

                1401    1353     490     302
 
AlwNI CAG_nnn'CTG

Cuts at:      0     754     943    2411    3466    3469    3546 
   Size:        754     189    1468    1055       3      77

  Fragments arranged by size: 

                1468    1055     754     189      77       3
 
ApaLI G'TGCA_C

Cuts at:      0    1838    3293    3546 
   Size:       1838    1455     253

  Fragments arranged by size: 

                1838    1455     253
 
ApoI r'AATT_y

Cuts at:      0     982    1101    1209    1336    1800    2869    3397    3546 
   Size:        982     119     108     127     464    1069     528     149

  Fragments arranged by size: 

                1069     982     528     464     149     127     119     108
            
 
AvaI C'yCGr_G

Cuts at:      0    3478    3546 
   Size:       3478      68
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     634     667    3182    3215    3546 
   Size:        634      33    2515      33     331

  Fragments arranged by size: 

                2515     634     331      33      33
 
BamHI G'GATC_C

Cuts at:      0      62    1855    3546 
   Size:         62    1793    1691

  Fragments arranged by size: 

                1793    1691      62
 
BanI G'GyrC_C

Cuts at:      0     468     518    2136    3546 
   Size:        468      50    1618    1410

  Fragments arranged by size: 

                1618    1410     468      50
 
BanII G_rGCy'C

Cuts at:      0      41     289    1576    3546 
   Size:         41     248    1287    1970

  Fragments arranged by size: 

                1970    1287     248      41
 
BbvCI CC'TCA_GC

Cuts at:      0      50    1658    3546 
   Size:         50    1608    1888

  Fragments arranged by size: 

                1888    1608      50
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2118    2152    3546 
   Size:       2118      34    1394

  Fragments arranged by size: 

                2118    1394      34
 
BclI T'GATC_A

Cuts at:      0     156    3546 
   Size:        156    3390
 
BglI GCCn_nnn'nGGC

Cuts at:      0    2736    3172    3546 
   Size:       2736     436     374

  Fragments arranged by size: 

                2736     436     374
 
BglII A'GATC_T

Cuts at:      0    1224    3546 
   Size:       1224    2322
 
BlpI GC'TnA_GC

Cuts at:      0     559    3546 
   Size:        559    2987
 
Bme1580I G_kGCm'C

Cuts at:      0    1785    1842    2691    2949    3092    3297    3314    3479 
   Size:       1785      57     849     258     143     205      17     165

Cuts at:   3479    3546 
   Size:         67

  Fragments arranged by size: 

                1785     849     258     205     165     143      67      57
                  17
 
BmgBI CAC'GTC

Cuts at:      0     646    2835    3546 
   Size:        646    2189     711

  Fragments arranged by size: 

                2189     711     646
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     484    2290    3546 
   Size:        484    1806    1256

  Fragments arranged by size: 

                1806    1256     484
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2000    3252    3546 
   Size:       2000    1252     294

  Fragments arranged by size: 

                2000    1252     294
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      25     224    1325    1349    1620    2413    2612    2666 
   Size:         25     199    1101      24     271     793     199      54

Cuts at:   2666    2726    2729    2810    2927    3546 
   Size:         60       3      81     117     619

  Fragments arranged by size: 

                1101     793     619     271     199     199     117      81
                  60      54      25      24       3
 
Bpu10I CC'TnA_GC

Cuts at:      0      50     631    1658    3360    3546 
   Size:         50     581    1027    1702     186

  Fragments arranged by size: 

                1702    1027     581     186      50
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    3116    3546 
   Size:       3116     430
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     121    2532    3546 
   Size:        121    2411    1014

  Fragments arranged by size: 

                2411    1014     121
 
BsaHI Gr'CG_yC

Cuts at:      0     914    1400    3546 
   Size:        914     486    2146

  Fragments arranged by size: 

                2146     914     486
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     727     757    3546 
   Size:        727      30    2789

  Fragments arranged by size: 

                2789     727      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1671    1675    2579    3546 
   Size:       1671       4     904     967

  Fragments arranged by size: 

                1671     967     904       4
 
BseYI C'CCAG_C

Cuts at:      0     149     464     783    1860    1894    2117    2221    3106 
   Size:        149     315     319    1077      34     223     104     885

Cuts at:   3106    3546 
   Size:        440

  Fragments arranged by size: 

                1077     885     440     319     315     223     149     104
                  34
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      98     563    2857    3546 
   Size:         98     465    2294     689

  Fragments arranged by size: 

                2294     689     465      98
 
BsiEI CG_ry'CG

Cuts at:      0      96    3546 
   Size:         96    3450
 
BsiHKAI G_wGCw'C

Cuts at:      0     289    1842    3297    3546 
   Size:        289    1553    1455     249

  Fragments arranged by size: 

                1553    1455     289     249
 
BsmI GAATG_Cn'

Cuts at:      0    1129    3546 
   Size:       1129    2417
 
Bsp1286I G_dGCh'C

Cuts at:      0      41     289    1576    1785    1842    2691    2949    3092 
   Size:         41     248    1287     209      57     849     258     143

Cuts at:   3092    3297    3314    3479    3546 
   Size:        205      17     165      67

  Fragments arranged by size: 

                1287     849     258     248     209     205     165     143
                  67      57      41      17
 
BspHI T'CATG_A

Cuts at:      0    1967    3546 
   Size:       1967    1579
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1712    2284    3178    3546 
   Size:       1712     572     894     368

  Fragments arranged by size: 

                1712     894     572     368
 
BsrDI GCAATG_nn'

Cuts at:      0    1193    2399    3546 
   Size:       1193    1206    1147

  Fragments arranged by size: 

                1206    1193    1147
 
BsrFI r'CCGG_y

Cuts at:      0     130    3546 
   Size:        130    3416
 
BsrGI T'GTAC_A

Cuts at:      0     732    3516    3546 
   Size:        732    2784      30

  Fragments arranged by size: 

                2784     732      30
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1789    3546 
   Size:       1789    1757
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     174    3546 
   Size:        174    3372
 
BstYI r'GATC_y

Cuts at:      0      62    1224    1855    2601    3546 
   Size:         62    1162     631     746     945

  Fragments arranged by size: 

                1162     945     746     631      62
 
Bsu36I CC'TnA_GG

Cuts at:      0    1520    1535    2364    3546 
   Size:       1520      15     829    1182

  Fragments arranged by size: 

                1520    1182     829      15
 
BtgI C'CryG_G

Cuts at:      0      57     522     968    3546 
   Size:         57     465     446    2578

  Fragments arranged by size: 

                2578     465     446      57
 
BtsI GCAGTG_nn'

Cuts at:      0      81     546     671    1864    2216    2217    3092    3546 
   Size:         81     465     125    1193     352       1     875     454

  Fragments arranged by size: 

                1193     875     465     454     352     125      81       1
            
 
DraIII CAC_nnn'GTG

Cuts at:      0     541    2790    3546 
   Size:        541    2249     756

  Fragments arranged by size: 

                2249     756     541
 
EaeI y'GGCC_r

Cuts at:      0      93    1166    3173    3546 
   Size:         93    1073    2007     373

  Fragments arranged by size: 

                2007    1073     373      93
 
EagI C'GGCC_G

Cuts at:      0      93    3546 
   Size:         93    3453
 
EarI CTCTTCn'nnn_

Cuts at:      0     337    1130    1643    1969    2460    2462    2582    3005 
   Size:        337     793     513     326     491       2     120     423

Cuts at:   3005    3546 
   Size:        541

  Fragments arranged by size: 

                 793     541     513     491     423     337     326     120
                   2
 
EcoICRI GAG'CTC

Cuts at:      0     287    3546 
   Size:        287    3259
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      25     224     696    1325    1349    1620    1667    1765 
   Size:         25     199     472     629      24     271      47      98

Cuts at:   1765    1941    2413    2612    2666    2726    2729    2810    2927 
   Size:        176     472     199      54      60       3      81     117

Cuts at:   2927    3443    3546 
   Size:        516     103

  Fragments arranged by size: 

                 629     516     472     472     271     199     199     176
                 117     103      98      81      60      54      47      25
                  24       3
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1474    3546 
   Size:       1474    2072
 
EcoO109I rG'GnC_Cy

Cuts at:      0      86     105    1586    2622    3261    3546 
   Size:         86      19    1481    1036     639     285

  Fragments arranged by size: 

                1481    1036     639     285      86      19
 
EcoRI G'AATT_C

Cuts at:      0     982    3546 
   Size:        982    2564
 
EcoRV GAT'ATC

Cuts at:      0    1504    3546 
   Size:       1504    2042
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     315    1968    3546 
   Size:        315    1653    1578

  Fragments arranged by size: 

                1653    1578     315
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1573    1605    2086    2118    3271    3303    3343    3375 
   Size:       1573      32     481      32    1153      32      40      32

Cuts at:   3375    3546 
   Size:        171

  Fragments arranged by size: 

                1573    1153     481     171      40      32      32      32
                  32
 
HindIII A'AGCT_T

Cuts at:      0     221    1610    3546 
   Size:        221    1389    1936

  Fragments arranged by size: 

                1936    1389     221
 
KpnI G_GTAC'C

Cuts at:      0     522    3546 
   Size:        522    3024
 
MscI TGG'CCA

Cuts at:      0    1168    3175    3546 
   Size:       1168    2007     371

  Fragments arranged by size: 

                2007    1168     371
 
MslI CAynn'nnrTG

Cuts at:      0     490     792    2093    2145    3546 
   Size:        490     302    1301      52    1401

  Fragments arranged by size: 

                1401    1301     490     302      52
 
MspA1I CmG'CkG

Cuts at:      0      59     170     547     618    2433    2656    2830    3226 
   Size:         59     111     377      71    1815     223     174     396

Cuts at:   3226    3324    3345    3466    3546 
   Size:         98      21     121      80

  Fragments arranged by size: 

                1815     396     377     223     174     121     111      98
                  80      71      59      21
 
NcoI C'CATG_G

Cuts at:      0     522     968    3546 
   Size:        522     446    2578

  Fragments arranged by size: 

                2578     522     446
 
NspI r_CATG'y

Cuts at:      0    1092    1562    1928    2092    2496    3546 
   Size:       1092     470     366     164     404    1050

  Fragments arranged by size: 

                1092    1050     470     404     366     164
 
PasI CC'CwG_GG

Cuts at:      0     610    3546 
   Size:        610    2936
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    2719    3546 
   Size:       2719     827
 
PpuMI rG'GwC_Cy

Cuts at:      0     105    2622    3546 
   Size:        105    2517     924

  Fragments arranged by size: 

                2517     924     105
 
PshAI GACnn'nnGTC

Cuts at:      0     531    3546 
   Size:        531    3015
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     846     878    3546 
   Size:        846      32    2668

  Fragments arranged by size: 

                2668     846      32
 
PstI C_TGCA'G

Cuts at:      0     678    1002    1149    2981    3033    3443    3546 
   Size:        678     324     147    1832      52     410     103

  Fragments arranged by size: 

                1832     678     410     324     147     103      52
 
PvuII CAG'CTG

Cuts at:      0     170     547     618    2433    2656    2830    3324    3345 
   Size:        170     377      71    1815     223     174     494      21

Cuts at:   3345    3466    3546 
   Size:        121      80

  Fragments arranged by size: 

                1815     494     377     223     174     170     121      80
                  71      21
 
SacI G_AGCT'C

Cuts at:      0     289    3546 
   Size:        289    3257
 
SacII CC_GC'GG

Cuts at:      0      60    3546 
   Size:         60    3486
 
SanDI GG'GwC_CC

Cuts at:      0     105    3546 
   Size:        105    3441
 
SapI GCTCTTCn'nnn_

Cuts at:      0    2582    3005    3546 
   Size:       2582     423     541

  Fragments arranged by size: 

                2582     541     423
 
SbfI CC_TGCA'GG

Cuts at:      0    3443    3546 
   Size:       3443     103
 
ScaI AGT'ACT

Cuts at:      0    1195    3546 
   Size:       1195    2351
 
SfcI C'TryA_G

Cuts at:      0     657     674     806     998    1145    2083    2977    3005 
   Size:        657      17     132     192     147     938     894      28

Cuts at:   3005    3029    3439    3448    3546 
   Size:         24     410       9      98

  Fragments arranged by size: 

                 938     894     657     410     192     147     132      98
                  28      24      17       9
 
SmaI CCC'GGG

Cuts at:      0    3480    3546 
   Size:       3480      66
 
SmlI C'TyrA_G

Cuts at:      0    1287    3131    3546 
   Size:       1287    1844     415

  Fragments arranged by size: 

                1844    1287     415
 
SphI G_CATG'C

Cuts at:      0    1562    2092    3546 
   Size:       1562     530    1454

  Fragments arranged by size: 

                1562    1454     530
 
StuI AGG'CCT

Cuts at:      0    1846    3546 
   Size:       1846    1700
 
StyI C'CwwG_G

Cuts at:      0     290     408     522     719     968    2335    2663    2750 
   Size:        290     118     114     197     249    1367     328      87

Cuts at:   2750    3546 
   Size:        796

  Fragments arranged by size: 

                1367     796     328     290     249     197     118     114
                  87
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     163     456     804    1908    2157    2219    2823    2933 
   Size:        163     293     348    1104     249      62     604     110

Cuts at:   2933    3546 
   Size:        613

  Fragments arranged by size: 

                1104     613     604     348     293     249     163     110
                  62
 
TatI w'GTAC_w

Cuts at:      0     732    1051    1193    3516    3546 
   Size:        732     319     142    2323      30

  Fragments arranged by size: 

                2323     732     319     142      30
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     727     759    2562    2594    3546 
   Size:        727      32    1803      32     952

  Fragments arranged by size: 

                1803     952     727      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    2445    3546 
   Size:       2445    1101
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1902    2430    3321    3546 
   Size:       1902     528     891     225

  Fragments arranged by size: 

                1902     891     528     225
 
XmaI C'CCGG_G

Cuts at:      0    3478    3546 
   Size:       3478      68
 
XmnI GAAnn'nnTTC

Cuts at:      0    1210    2869    3546 
   Size:       1210    1659     677

  Fragments arranged by size: 

                1659    1210     677
 
ZraI GAC'GTC

Cuts at:      0    1401    3546 
   Size:       1401    2145
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AcuI    AflII   AflIII     AhdI     AleI
    AlwNI    ApaLI     ApoI     AvaI     BaeI    BamHI     BanI    BanII
    BbvCI     BcgI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaHI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspHI
    BspMI    BsrDI    BsrFI    BsrGI   BstAPI    BstXI    BstYI   Bsu36I
     BtgI     BtsI   DraIII     EaeI     EagI     EarI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI    Hin4I  HindIII     KpnI
     MscI     MslI   MspA1I     NcoI     NspI     PasI    PflMI    PpuMI
    PshAI     PsrI     PstI    PvuII     SacI    SacII    SanDI     SapI
     SbfI     ScaI     SfcI     SmaI     SmlI     SphI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XcmI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AclI     AfeI     AgeI     AlfI     AloI     ApaI     AscI
     AseI    AsiSI    AvrII     BbeI     BbsI    BciVI    BfrBI     BmtI
    BsaAI    BsaBI    BsaWI    BsiWI    BsmBI    BspEI    BsrBI   BssHII
    BssSI    BstBI   BstEII  BstZ17I    BtgZI     ClaI    CspCI     DraI
     DrdI     EciI     FseI     FspI    FspAI    HaeII   HincII     HpaI
     KasI     MfeI     MluI     MmeI     NaeI     NarI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PciI     PfoI     PmeI
     PmlI     PpiI     PsiI   PspOMI    PspXI     PvuI    RsrII     SalI
    SexAI     SfiI     SfoI     SgfI    SgrAI    SnaBI     SpeI     SrfI
     SspI     SwaI     XbaI     XhoI
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