Restriction map for pF1KE1756
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# NO CUT ENZYMES (  175 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AfaI        AflII       AgeI        
AhdI        Alw44I      AocI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspHI       AspI        AvaI        
AvaII       AviII       AvrII       BalI        BamHI       
BanI        BanII       BbeI        BbrPI       BbsI        
BbvCI       BcgI        BfaI        BfrI        BglI        
BglII       BlnI        BlpI        BmyI        BpmI        
Bpu1102I    BpuAI       BsaAI       BsaHI       BsaI        
BsaWI       BseAI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsmI        BsoBI       Bsp1286I    Bsp1407I    
BspDI       BspEI       BspMI       BsrBI       BsrDI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstBI       BstF5I      BstUI       BstZ17I     Bsu36I      
CelII       CfoI        Cfr10I      Cfr13I      ClaI        
CpoI        DraI        DraII       DraIII      DrdI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoRI       
EcoRV       EcoT22I     FokI        FseI        FspI        
HaeII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HpaI        KasI        KpnI        
Ksp632I     KspI        MaeI        MfeI        MluI        
MluNI       MroI        MscI        MunI        MvnI        
NaeI        NarI        NdeI        NgoMIV      NheI        
NlaIV       NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        RsaI        
RsrII       SacI        SacII       SalI        SapI        
Sau96I      SbfI        ScaI        SfcI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        Tth111I     Van91I      
XbaI        XhoI        XmaCI       XmaI        XmnI        


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# ONE CUT ENZYMES (   42 enzymes) #
###################################

AcsI           65  AAATTT               [GA]^AATT[CT]
AflIII        372  ACATGT               A^C[GA][CT]GT
AlwNI         573  CAGCCTCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApoI           65  AAATTT               [GA]^AATT[CT]
BbvI          622  GCTGC                GCAGC(8/12)
BciVI         430  GGATAC               GTATCC(6/5)
BclI          348  TGATCA               T^GATCA
BcnI          592  CCCGG                CC^[GC]GG
BsiYI          30  CCGATCTGCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI           30  CCGATCTGCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI         310  GAGAC                GTCTC(1/5)
BsmBI         310  GAGACG               CGTCTC(1/5)
BsmFI         588  GTCCC                GGGAC(10/14)
BspLU11I      372  ACATGT               A^CATGT
BstAPI        118  GCACTTTTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        456  GGTAACC              G^GT[ACGT]ACC
BstPI         456  GGTAACC              G^GT[ACGT]ACC
BstYI         229  AGATCC               [GA]^GATC[CT]
DdeI          616  CTCAG                C^T[ACGT]AG
DsaI          744  CCATGG               C^C[GA][CT]GG
EaeI           38  CGGCCA               [CT]^GGCC[GA]
EcoO65I       456  GGTAACC              G^GT[ACGT]ACC
EcoT14I       744  CCATGG               C^C[AT][AT]GG
FauI          653  GCGGG                CCCGC(4/6)
FbaI          348  TGATCA               T^GATCA
HapII         593  CCGG                 C^CGG
HindIII       545  AAGCTT               A^AGCTT
HpaII         593  CCGG                 C^CGG
MaeII         760  ACGT                 A^CGT
MflI          229  AGATCC               [GA]^GATC[CT]
MspA1I        478  CAGCTG               C[AC]G^C[GT]G
MspI          593  CCGG                 C^CGG
NciI          592  CCCGG                CC^[GC]GG
NcoI          744  CCATGG               C^CATGG
NspI          372  ACATGT               [GA]CATG^[CT]
PvuII         478  CAGCTG               CAG^CTG
SexAI         696  ACCAGGT              A^CC[AT]GGT
SfaNI         212  GATGC                GCATC(5/9)
StyI          744  CCATGG               C^C[AT][AT]GG
TseI          622  GCTGC                G^C[AT]GC
XcmI          735  CCAAGTGAGCCATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         229  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   13 enzymes) #
###################################

AlwI          230  GATCC                GGATC(4/5)
              727  GATCC               
BsaBI         230  GATCCTTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              349  GATCAGAATC          
BspHI         177  TCATGA               T^CATGA
              194  TCATGA              
BstNI         462  CCTGG                CC^[AT]GG
              697  CCAGG               
EcoRII        462  CCTGG                ^CC[AT]GG
              697  CCAGG               
HaeIII         39  GGCC                 GG^CC
              387  GGCC                
MamI          230  GATCCTTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              349  GATCAGAATC          
MslI           54  CATCAGCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
               99  CACCCAGATG          
MvaI          462  CCTGG                CC^[AT]GG
              697  CCAGG               
MwoI          118  GCACTTTTTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              734  GCCAAGTGAGC         
PleI          500  GACTC                GAGTC(4/5)
              552  GAGTC               
RcaI          177  TCATGA               T^CATGA
              194  TCATGA              
Tsp45I        523  GTCAC                ^GT[GC]AC
              721  GTGAC               


#########################################
# 3 OR MORE CUT ENZYMES (   28 enzymes) #
#########################################

AciI        AluI        BsaJI       BseRI       BsrI        
BssKI       BstXI       Cac8I       DpnII       Fnu4HI      
HinfI       HphI        ItaI        MaeIII      MboI        
MboII       MnlI        MseI        NdeII       NlaIII      
Sau3AI      ScrFI       TaqI        TfiI        Tru9I       
Tsp509I     TspRI       TthHB8I     
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