Restriction map for pF1KE6772
##################################
# NO CUT ENZYMES (  103 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AflII       AflIII      AgeI        AhdI        Alw44I      
Aor51HI     ApaLI       AscI        AseI        AsnI        
Asp718I     AspEI       AviII       AvrII       BbrPI       
BcgI        BfrI        BlnI        BsaAI       BsaBI       
BsaI        BseAI       BsiEI       BsiWI       BsmBI       
Bsp1407I    BspEI       BspLU11I    BsrBI       BsrDI       
BsrGI       BssHII      BssSI       Bst1107I    BstBI       
BstEII      BstPI       BstXI       BstZ17I     DraI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
EcoNI       EcoO65I     EcoRI       EcoRV       EcoT22I     
FseI        FspI        HincII      HindII      HpaI        
KpnI        KspI        MaeII       MamI        MfeI        
MluI        MroI        MunI        NaeI        NdeI        
NgoMIV      NotI        NruI        NsiI        NspV        
PacI        PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        Sse8387I    SspBI       SspI        
SwaI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   73 enzymes) #
###################################

AccII         339  CGCG                 CG^CG
AcsI          293  GAATTT               [GA]^AATT[CT]
AcyI          381  GGCGCC               G[GA]^CG[CT]C
AocI         1701  CCTCAGG              CC^T[ACGT]AGG
ApaI         1458  GGGCCC               GGGCC^C
ApoI          293  GAATTT               [GA]^AATT[CT]
Asp700I       985  GAAGTGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI         1599  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI         1217  TGGCCA               TGG^CCA
BamHI         459  GGATCC               G^GATCC
BbeI          381  GGCGCC               GGCGC^C
BbsI         1834  GAAGAC               GAAGAC(2/6)
BbvCI        1041  CCTCAGC              CCTCAGC(-5/-2)
BciVI        1580  GTATCC               GTATCC(6/5)
BclI         1365  TGATCA               T^GATCA
BglI         1422  GCCGTACTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        1797  AGATCT               A^GATCT
BlpI         1417  GCTAAGC              GC^T[ACGT]AGC
Bpu1102I     1417  GCTAAGC              GC^T[ACGT]AGC
BpuAI        1834  GAAGAC               GAAGAC(2/6)
BsaHI         381  GGCGCC               G[GA]^CG[CT]C
BsaWI           5  TCCGGT               [AT]^CCGG[AT]
BseRI         518  CTCCTC               GAGGAG(10/8)
BsgI         1535  CTGCAC               GTGCAG(16/14)
BsmFI        1706  GGGAC                GGGAC(10/14)
BsmI         1751  GAATGC               GAATGC(1/-1)
BspDI         190  ATCGAT               AT^CGAT
BspHI        1830  TCATGA               T^CATGA
BspMI         647  ACCTGC               ACCTGC(4/8)
BsrFI          95  ACCGGC               [GA]^CCGG[CT]
BstAPI       1306  GCAGGAGATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI         339  CGCG                 CG^CG
Bsu36I       1701  CCTCAGG              CC^T[ACGT]AGG
CelII        1417  GCTAAGC              GC^T[ACGT]AGC
CfoI          382  GCGC                 GCG^C
Cfr10I         95  ACCGGC               [GA]^CCGG[CT]
ClaI          190  ATCGAT               AT^CGAT
CpoI           61  CGGACCG              CG^G[AT]CCG
DraIII       1177  CACATAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI         1055  CCATGG               C^C[GA][CT]GG
EaeI         1217  TGGCCA               [CT]^GGCC[GA]
Eco81I       1701  CCTCAGG              CC^T[ACGT]AGG
FbaI         1365  TGATCA               T^GATCA
HaeII         381  GGCGCC               [GA]GCGC^[CT]
HgaI           71  GCGTC                GACGC(5/10)
HhaI          382  GCGC                 GCG^C
Hin1I         381  GGCGCC               G[GA]^CG[CT]C
HinP1I        382  GCGC                 G^CGC
HindIII       873  AAGCTT               A^AGCTT
KasI          381  GGCGCC               G^GCGCC
MluNI        1217  TGGCCA               TGG^CCA
MscI         1217  TGGCCA               TGG^CCA
MvnI          339  CGCG                 CG^CG
NarI          381  GGCGCC               GG^CGCC
NcoI         1055  CCATGG               C^CATGG
NheI          163  GCTAGC               G^CTAGC
PaeR7I        968  CTCGAG               C^TCGAG
PflFI        1599  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        1176  CCACATAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI         644  AGGACCT              [GA]G^G[AT]CC[CT]
PstI          434  CTGCAG               CTGCA^G
RcaI         1830  TCATGA               T^CATGA
RsrII          61  CGGACCG              CG^G[AT]CCG
SfcI          434  CTGCAG               C^T[GA][CT]AG
SfoI          381  GGCGCC               GGC^GCC
SphI         1318  GCATGC               GCATG^C
Tsp45I        559  GTCAC                ^GT[GC]AC
Tth111I      1599  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I       1176  CCACATAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI          135  TCTAGA               T^CTAGA
XcmI         1205  CCAATGTTGAGTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI          968  CTCGAG               C^TCGAG
XmnI          985  GAAGTGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AspHI         950  GTGCTC               G[AT]GC[AT]^C
             1357  GAGCAC              
BfaI          136  CTAG                 C^TAG
              164  CTAG                
BsiHKAI       950  GTGCTC               G[AT]GC[AT]^C
             1357  GAGCAC              
DraII         644  AGGACCT              [GA]G^G[ACGT]CC[CT]
             1458  GGGCCCC             
DrdI          550  GACCCAAATGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1708  GACAAAGTGGTC        
EarI          997  CTCTTC               CTCTTC(1/4)
              831  GAAGAG              
EcoO109I      644  AGGACCT              [GA]G^G[ACGT]CC[CT]
             1458  GGGCCCC             
FauI           25  GCGGG                CCCGC(4/6)
             1350  GCGGG               
Ksp632I       997  CTCTTC               CTCTTC(1/4)
              831  GAAGAG              
MaeI          136  CTAG                 C^TAG
              164  CTAG                
MaeIII        559  GTCAC                ^GT[ACGT]AC
             1134  GTTAC               
MslI          981  CACAGAAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1296  CATCTGGGTG          
SmlI          371  CTCAAG               C^T[CT][GA]AG
              968  CTCGAG              
StuI           15  AGGCCT               AGG^CCT
             1610  AGGCCT              
TaqI          191  TCGA                 T^CGA
              969  TCGA                
TfiI          575  GATTC                G^A[AT]TC
              990  GATTC               
TthHB8I       191  TCGA                 T^CGA
              969  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   65 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AlwNI       
AvaI        AvaII       BanI        BanII       BbvI        
BcnI        BmyI        BpmI        BsaJI       BsiYI       
BslI        BsmAI       BsoBI       Bsp1286I    BsrI        
BssKI       BstF5I      BstNI       BstYI       Cac8I       
Cfr13I      DdeI        DpnII       EcoRII      EcoT14I     
Fnu4HI      FokI        HaeIII      HapII       HinfI       
HpaII       HphI        ItaI        MboI        MboII       
MflI        MnlI        MseI        MspA1I      MspI        
MvaI        MwoI        NciI        NdeII       NlaIII      
NlaIV       NspI        PleI        PvuII       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       StyI        
Tru9I       TseI        Tsp509I     TspRI       XhoII       
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