Restriction map for pF1KE6772
 (Linear) (Six-base) MAPSORT of: KIEE6772.seq  Check: 2101  from: 1  to: 1839



KIEE6772.orf

 With 182 enzymes: SgfI * 

                        July 24, 2018 16:24  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     335     509    1839 
   Size:        335     174    1330

  Fragments arranged by size: 

                1330     335     174
 
AleI CACnn'nnGTG

Cuts at:      0     985    1839 
   Size:        985     854
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     350     382    1839 
   Size:        350      32    1457

  Fragments arranged by size: 

                1457     350      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0      45     648    1049    1576    1839 
   Size:         45     603     401     527     263

  Fragments arranged by size: 

                 603     527     401     263      45
 
ApaI G_GGCC'C

Cuts at:      0    1462    1839 
   Size:       1462     377
 
ApoI r'AATT_y

Cuts at:      0     293    1839 
   Size:        293    1546
 
AvaI C'yCGr_G

Cuts at:      0      81     455     968    1003    1839 
   Size:         81     374     513      35     836

  Fragments arranged by size: 

                 836     513     374      81      35
 
BamHI G'GATC_C

Cuts at:      0     459    1839 
   Size:        459    1380
 
BanI G'GyrC_C

Cuts at:      0      48     241     381    1839 
   Size:         48     193     140    1458

  Fragments arranged by size: 

                1458     193     140      48
 
BanII G_rGCy'C

Cuts at:      0     456    1205    1462    1839 
   Size:        456     749     257     377

  Fragments arranged by size: 

                 749     456     377     257
 
BbeI G_GCGC'C

Cuts at:      0     385    1839 
   Size:        385    1454
 
BbvCI CC'TCA_GC

Cuts at:      0    1042    1839 
   Size:       1042     797
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1591    1839 
   Size:       1591     248
 
BclI T'GATC_A

Cuts at:      0    1365    1839 
   Size:       1365     474
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1428    1839 
   Size:       1428     411
 
BglII A'GATC_T

Cuts at:      0    1797    1839 
   Size:       1797      42
 
BlpI GC'TnA_GC

Cuts at:      0    1418    1839 
   Size:       1418     421
 
Bme1580I G_kGCm'C

Cuts at:      0      53     982    1462    1839 
   Size:         53     929     480     377

  Fragments arranged by size: 

                 929     480     377      53
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     695    1839 
   Size:        695    1144
 
BmtI G_CTAG'C

Cuts at:      0     167    1839 
   Size:        167    1672
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     127     839    1219    1415    1430    1467    1839 
   Size:        127     712     380     196      15      37     372

  Fragments arranged by size: 

                 712     380     372     196     127      37      15
 
Bpu10I CC'TnA_GC

Cuts at:      0     497     641    1042    1839 
   Size:        497     144     401     797

  Fragments arranged by size: 

                 797     497     401     144
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     356    1839 
   Size:        356    1483
 
BsaHI Gr'CG_yC

Cuts at:      0     382    1839 
   Size:        382    1457
 
BsaWI w'CCGG_w

Cuts at:      0       5    1839 
   Size:          5    1834
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     350     380     812     842    1614    1644    1839 
   Size:        350      30     432      30     772      30     195

  Fragments arranged by size: 

                 772     432     350     195      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     509    1839 
   Size:        509    1330
 
BseYI C'CCAG_C

Cuts at:      0    1633    1839 
   Size:       1633     206
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1520    1839 
   Size:       1520     319
 
BsiHKAI G_wGCw'C

Cuts at:      0     954    1361    1839 
   Size:        954     407     478

  Fragments arranged by size: 

                 954     478     407
 
BsmI GAATG_Cn'

Cuts at:      0    1757    1839 
   Size:       1757      82
 
Bsp1286I G_dGCh'C

Cuts at:      0      53     456     954     982    1205    1361    1462    1839 
   Size:         53     403     498      28     223     156     101     377

  Fragments arranged by size: 

                 498     403     377     223     156     101      53      28
            
 
BspHI T'CATG_A

Cuts at:      0    1830    1839 
   Size:       1830       9
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     656    1839 
   Size:        656    1183
 
BsrFI r'CCGG_y

Cuts at:      0      95    1839 
   Size:         95    1744
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1312    1839 
   Size:       1312     527
 
BstYI r'GATC_y

Cuts at:      0     442     459     492    1025    1797    1839 
   Size:        442      17      33     533     772      42

  Fragments arranged by size: 

                 772     533     442      42      33      17
 
Bsu36I CC'TnA_GG

Cuts at:      0    1702    1839 
   Size:       1702     137
 
BtgI C'CryG_G

Cuts at:      0    1055    1839 
   Size:       1055     784
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     135    1839 
   Size:        135    1704
 
BtsI GCAGTG_nn'

Cuts at:      0     568    1131    1839 
   Size:        568     563     708

  Fragments arranged by size: 

                 708     568     563
 
ClaI AT'CG_AT

Cuts at:      0     191    1839 
   Size:        191    1648
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1209    1244    1839 
   Size:       1209      35     595

  Fragments arranged by size: 

                1209     595      35
 
DraIII CAC_nnn'GTG

Cuts at:      0    1182    1839 
   Size:       1182     657
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     556    1714    1839 
   Size:        556    1158     125

  Fragments arranged by size: 

                1158     556     125
 
EaeI y'GGCC_r

Cuts at:      0    1217    1839 
   Size:       1217     622
 
EarI CTCTTCn'nnn_

Cuts at:      0     826    1003    1839 
   Size:        826     177     836

  Fragments arranged by size: 

                 836     826     177
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     127     335     509     839    1219    1415    1430    1467 
   Size:        127     208     174     330     380     196      15      37

Cuts at:   1467    1839 
   Size:        372

  Fragments arranged by size: 

                 380     372     330     208     196     174     127      37
                  15
 
EcoO109I rG'GnC_Cy

Cuts at:      0     645    1459    1839 
   Size:        645     814     380

  Fragments arranged by size: 

                 814     645     380
 
HaeII r_GCGC'y

Cuts at:      0     385    1839 
   Size:        385    1454
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1588    1620    1839 
   Size:       1588      32     219

  Fragments arranged by size: 

                1588     219      32
 
HindIII A'AGCT_T

Cuts at:      0     873    1839 
   Size:        873     966
 
KasI G'GCGC_C

Cuts at:      0     381    1839 
   Size:        381    1458
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1095    1839 
   Size:       1095     744
 
MscI TGG'CCA

Cuts at:      0    1219    1839 
   Size:       1219     620
 
MslI CAynn'nnrTG

Cuts at:      0     985    1300    1839 
   Size:        985     315     539

  Fragments arranged by size: 

                 985     539     315
 
MspA1I CmG'CkG

Cuts at:      0     424     705    1189    1839 
   Size:        424     281     484     650

  Fragments arranged by size: 

                 650     484     424     281
 
NarI GG'CG_CC

Cuts at:      0     382    1839 
   Size:        382    1457
 
NcoI C'CATG_G

Cuts at:      0    1055    1839 
   Size:       1055     784
 
NheI G'CTAG_C

Cuts at:      0     163    1839 
   Size:        163    1676
 
NspI r_CATG'y

Cuts at:      0     130     513    1322    1839 
   Size:        130     383     809     517

  Fragments arranged by size: 

                 809     517     383     130
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1182    1839 
   Size:       1182     657
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     350     382    1839 
   Size:        350      32    1457

  Fragments arranged by size: 

                1457     350      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     645    1839 
   Size:        645    1194
 
PspOMI G'GGCC_C

Cuts at:      0    1458    1839 
   Size:       1458     381
 
PspXI vC'TCGA_Gb

Cuts at:      0     968    1839 
   Size:        968     871
 
PstI C_TGCA'G

Cuts at:      0     438    1839 
   Size:        438    1401
 
PvuII CAG'CTG

Cuts at:      0     424     705    1189    1839 
   Size:        424     281     484     650

  Fragments arranged by size: 

                 650     484     424     281
 
RsrII CG'GwC_CG

Cuts at:      0      62    1839 
   Size:         62    1777
 
SfcI C'TryA_G

Cuts at:      0     434    1839 
   Size:        434    1405
 
SfoI GGC'GCC

Cuts at:      0     383    1839 
   Size:        383    1456
 
SmlI C'TyrA_G

Cuts at:      0     371     968    1839 
   Size:        371     597     871

  Fragments arranged by size: 

                 871     597     371
 
SphI G_CATG'C

Cuts at:      0    1322    1839 
   Size:       1322     517
 
StuI AGG'CCT

Cuts at:      0      17    1612    1839 
   Size:         17    1595     227

  Fragments arranged by size: 

                1595     227      17
 
StyI C'CwwG_G

Cuts at:      0      53     930     974    1055    1839 
   Size:         53     877      44      81     784

  Fragments arranged by size: 

                 877     784      81      53      44
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1290    1839 
   Size:       1290     549
 
TatI w'GTAC_w

Cuts at:      0     699    1839 
   Size:        699    1140
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     812     844    1839 
   Size:        812      32     995

  Fragments arranged by size: 

                 995     812      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1602    1839 
   Size:       1602     237
 
XbaI T'CTAG_A

Cuts at:      0     135    1839 
   Size:        135    1704
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1212    1839 
   Size:       1212     627
 
XhoI C'TCGA_G

Cuts at:      0     968    1839 
   Size:        968     871
 
XmnI GAAnn'nnTTC

Cuts at:      0     989    1839 
   Size:        989     850
 

 Enzymes that do cut:

     AcuI     AleI     AloI    AlwNI     ApaI     ApoI     AvaI    BamHI
     BanI    BanII     BbeI    BbvCI    BciVI     BclI     BglI    BglII
     BlpI Bme1580I     BmrI     BmtI     BpmI   Bpu10I    BpuEI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I
    BspHI    BspMI    BsrFI   BstAPI    BstYI   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI   DraIII     DrdI     EaeI     EarI  Eco57MI
 EcoO109I    HaeII    Hin4I  HindIII     KasI     MmeI     MscI     MslI
   MspA1I     NarI     NcoI     NheI     NspI    PflMI     PpiI    PpuMI
   PspOMI    PspXI     PstI    PvuII    RsrII     SfcI     SfoI     SmlI
     SphI     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI    ApaLI     AscI     AseI    AsiSI    AvrII
     BaeI     BbsI     BcgI    BfrBI    BmgBI     BplI     BsaI    BsaAI
    BsaBI    BsiEI    BsiWI    BsmBI    BspEI    BsrBI    BsrDI    BsrGI
   BssHII    BssSI    BstBI   BstEII    BstXI  BstZ17I     DraI     EagI
     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI   HincII     HpaI     KpnI     MfeI     MluI     NaeI     NdeI
   NgoMIV     NotI     NruI     NsiI     PacI     PasI     PciI     PfoI
     PmeI     PmlI    PshAI     PsiI     PsrI     PvuI     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SrfI     SspI     SwaI     XmaI
     ZraI
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