Restriction map for pF1KE2374
##################################
# NO CUT ENZYMES (  133 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AcyI        AflII       AgeI        AocI        Aor51HI     
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspI        AviII       AvrII       BalI        
BanI        BbeI        BbsI        BcgI        BciVI       
BclI        BfrI        BglI        BlnI        BlpI        
Bpu1102I    BpuAI       BsaHI       BsaI        BsaWI       
BseAI       BsiEI       BsiWI       BsmBI       BsmI        
Bsp1407I    BspDI       BspEI       BspLU11I    BsrDI       
BsrGI       BssHII      Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstXI       BstZ17I     Bsu36I      
Cac8I       CelII       ClaI        CpoI        DraI        
DrdI        EaeI        EagI        EclXI       Eco47III    
Eco52I      Eco81I      EcoO65I     EcoRI       EcoRV       
EcoT22I     FauI        FbaI        FseI        FspI        
HaeII       Hin1I       HincII      HindII      HpaI        
KasI        KpnI        MfeI        MluI        MluNI       
MroI        MscI        MseI        MunI        NaeI        
NarI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PflFI       PflMI       PinAI       PmeI        
PshAI       PshBI       Psp1406I    PvuI        RsrII       
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        Tru9I       Tsp509I     Tth111I     
Van91I      XbaI        XmnI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AccI          778  GTCTAC               GT^[AC][GT]AC
AflIII        338  ACACGT               A^C[GA][CT]GT
ApaI           51  GGGCCC               GGGCC^C
BamHI         156  GGATCC               G^GATCC
BbrPI         339  CACGTG               CAC^GTG
BbvCI         558  GCTGAGG              CCTCAGC(-5/-2)
BfaI          815  CTAG                 C^TAG
BglII         836  AGATCT               A^GATCT
BsaBI         819  GATCATGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI           15  GTGCAG               GTGCAG(16/14)
BspHI         821  TCATGA               T^CATGA
BspMI         716  ACCTGC               ACCTGC(4/8)
BsrFI         629  ACCGGC               [GA]^CCGG[CT]
BssSI         322  CTCGTG               CACGAG(-5/-1)
Cfr10I        629  ACCGGC               [GA]^CCGG[CT]
DraIII        148  CACAAAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI            7  CCGCGG               C^C[GA][CT]GG
EarI          756  GAAGAG               CTCTTC(1/4)
EcoT14I       107  CCAAGG               C^C[AT][AT]GG
HgaI           79  GCGTC                GACGC(5/10)
HindIII       289  AAGCTT               A^AGCTT
HphI          294  TCACC                GGTGA(8/7)
Ksp632I       756  GAAGAG               CTCTTC(1/4)
KspI            7  CCGCGG               CCGC^GG
MaeI          815  CTAG                 C^TAG
MaeIII         28  GTGAC                ^GT[ACGT]AC
MamI          819  GATCATGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
PaeR7I        502  CTCGAG               C^TCGAG
PmaCI         339  CACGTG               CAC^GTG
PmlI          339  CACGTG               CAC^GTG
PpuMI         540  GGGTCCC              [GA]G^G[AT]CC[CT]
RcaI          821  TCATGA               T^CATGA
SacI          679  GAGCTC               GAGCT^C
SacII           7  CCGCGG               CCGC^GG
SmaI           54  CCCGGG               CCC^GGG
StyI          107  CCAAGG               C^C[AT][AT]GG
TfiI          380  GATTC                G^A[AT]TC
Tsp45I         28  GTGAC                ^GT[GC]AC
XcmI          287  CCAAGCTTCACCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI          502  CTCGAG               C^TCGAG
XmaCI          54  CCCGGG               C^CCGGG
XmaI           54  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AfaI          441  GTAC                 GT^AC
              627  GTAC                
AhdI           73  GACGTTGCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              715  GACCTGCAGTC         
Alw44I        367  GTGCAC               G^TGCAC
              691  GTGCAC              
AlwNI         469  CAGATGCTG            CAG[ACGT][ACGT][ACGT]^CTG
              510  CAGCTTCTG           
ApaLI         367  GTGCAC               G^TGCAC
              691  GTGCAC              
AspEI          73  GACGTTGCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              715  GACCTGCAGTC         
AvaII         357  GGACC                G^G[AT]CC
              541  GGTCC               
BpmI          445  CTGGAG               CTGGAG(16/14)
              750  CTGGAG              
BsaAI         339  CACGTG               [CT]AC^GT[GA]
              685  TACGTG              
BseRI         499  CTCCTC               GAGGAG(10/8)
              730  GAGGAG              
BsmAI         733  GAGAC                GTCTC(1/5)
              723  GTCTC               
BsrBI         132  CCGCTC               CCGCTC(-3/-3)
              460  GAGCGG              
DdeI          384  CTCAG                C^T[ACGT]AG
              559  CTGAG               
Eam1105I       73  GACGTTGCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              715  GACCTGCAGTC         
EcoNI         267  CCTCACGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              297  CCTGGATGAGG         
MslI           21  CATCTCTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              295  CACCTGGATG          
PleI          735  GACTC                GAGTC(4/5)
              811  GAGTC               
RsaI          441  GTAC                 GT^AC
              627  GTAC                
SmlI          502  CTCGAG               C^T[CT][GA]AG
              574  CTCAAG              
TspRI         365  CAGTG                CA[GC]TG[ACGT][ACGT]^
              650  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   63 enzymes) #
#########################################

AccII       AciI        AluI        AlwI        AspHI       
AvaI        BanII       BbvI        BcnI        BmyI        
BsaJI       BsiHKAI     BsiYI       BslI        BsmFI       
BsoBI       Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       BstUI       BstYI       CfoI        Cfr13I      
DpnII       DraII       EcoO109I    EcoRII      Fnu4HI      
FokI        HaeIII      HapII       HhaI        HinP1I      
HinfI       HpaII       ItaI        MaeII       MboI        
MboII       MflI        MnlI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NdeII       
NlaIII      NlaIV       PstI        PvuII       Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        TaqI        
TseI        TthHB8I     XhoII       
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