Restriction map for pF1KE2374
 (Linear) (Six-base) MAPSORT of: KIEE2374.seq  Check: 9529  from: 1  to: 852



KIEE2374.orf

 With 182 enzymes: SgfI * 

                        September 6, 2011 14:42  ..

AccI GT'mk_AC

Cuts at:      0     779     852 
   Size:        779      73
 
AflIII A'CryG_T

Cuts at:      0     338     852 
   Size:        338     514
 
AhdI GACnn_n'nnGTC

Cuts at:      0      78     720     852 
   Size:         78     642     132

  Fragments arranged by size: 

                 642     132      78
 
AlwNI CAG_nnn'CTG

Cuts at:      0     474     515     852 
   Size:        474      41     337

  Fragments arranged by size: 

                 474     337      41
 
ApaI G_GGCC'C

Cuts at:      0      55     852 
   Size:         55     797
 
ApaLI G'TGCA_C

Cuts at:      0     367     691     852 
   Size:        367     324     161

  Fragments arranged by size: 

                 367     324     161
 
AvaI C'yCGr_G

Cuts at:      0      54     502     614     725     852 
   Size:         54     448     112     111     127

  Fragments arranged by size: 

                 448     127     112     111      54
 
BamHI G'GATC_C

Cuts at:      0     156     852 
   Size:        156     696
 
BanII G_rGCy'C

Cuts at:      0      55     615     683     852 
   Size:         55     560      68     169

  Fragments arranged by size: 

                 560     169      68      55
 
BbvCI CC'TCA_GC

Cuts at:      0     559     852 
   Size:        559     293
 
BglII A'GATC_T

Cuts at:      0     836     852 
   Size:        836      16
 
Bme1580I G_kGCm'C

Cuts at:      0      55     371     695     852 
   Size:         55     316     324     157

  Fragments arranged by size: 

                 324     316     157      55
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     618     852 
   Size:        618     234
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     466     771     852 
   Size:        466     305      81

  Fragments arranged by size: 

                 466     305      81
 
Bpu10I CC'TnA_GC

Cuts at:      0     559     852 
   Size:        559     293
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     559     852 
   Size:        559     293
 
BsaAI yAC'GTr

Cuts at:      0     341     687     852 
   Size:        341     346     165

  Fragments arranged by size: 

                 346     341     165
 
BsaBI GATnn'nnATC

Cuts at:      0     823     852 
   Size:        823      29
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     490     745     852 
   Size:        490     255     107

  Fragments arranged by size: 

                 490     255     107
 
BseYI C'CCAG_C

Cuts at:      0      45      67     396     764     852 
   Size:         45      22     329     368      88

  Fragments arranged by size: 

                 368     329      88      45      22
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      36     852 
   Size:         36     816
 
BsiHKAI G_wGCw'C

Cuts at:      0     371     683     695     852 
   Size:        371     312      12     157

  Fragments arranged by size: 

                 371     312     157      12
 
Bsp1286I G_dGCh'C

Cuts at:      0      55     371     615     683     695     852 
   Size:         55     316     244      68      12     157

  Fragments arranged by size: 

                 316     244     157      68      55      12
 
BspHI T'CATG_A

Cuts at:      0     821     852 
   Size:        821      31
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     725     852 
   Size:        725     127
 
BsrBI CCG'CTC

Cuts at:      0     134     462     852 
   Size:        134     328     390

  Fragments arranged by size: 

                 390     328     134
 
BsrFI r'CCGG_y

Cuts at:      0     629     852 
   Size:        629     223
 
BssSI C'ACGA_G

Cuts at:      0     322     852 
   Size:        322     530
 
BstYI r'GATC_y

Cuts at:      0     156     218     836     852 
   Size:        156      62     618      16

  Fragments arranged by size: 

                 618     156      62      16
 
BtgI C'CryG_G

Cuts at:      0       7     852 
   Size:          7     845
 
BtsI GCAGTG_nn'

Cuts at:      0     371     656     852 
   Size:        371     285     196

  Fragments arranged by size: 

                 371     285     196
 
DraIII CAC_nnn'GTG

Cuts at:      0     153     852 
   Size:        153     699
 
EarI CTCTTCn'nnn_

Cuts at:      0     751     852 
   Size:        751     101
 
EcoICRI GAG'CTC

Cuts at:      0     681     852 
   Size:        681     171
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     466     771     852 
   Size:        466     305      81

  Fragments arranged by size: 

                 466     305      81
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     271     301     852 
   Size:        271      30     551

  Fragments arranged by size: 

                 551     271      30
 
EcoO109I rG'GnC_Cy

Cuts at:      0      51      58     541     852 
   Size:         51       7     483     311

  Fragments arranged by size: 

                 483     311      51       7
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      64      96     160     192     706     738     828     852 
   Size:         64      32      64      32     514      32      90      24

  Fragments arranged by size: 

                 514      90      64      64      32      32      32      24
            
 
HindIII A'AGCT_T

Cuts at:      0     289     852 
   Size:        289     563
 
MslI CAynn'nnrTG

Cuts at:      0      25     299     852 
   Size:         25     274     553

  Fragments arranged by size: 

                 553     274      25
 
MspA1I CmG'CkG

Cuts at:      0       9      88     125     495     852 
   Size:          9      79      37     370     357

  Fragments arranged by size: 

                 370     357      79      37       9
 
PfoI T'CCnGG_A

Cuts at:      0     481     852 
   Size:        481     371
 
PmlI CAC'GTG

Cuts at:      0     341     852 
   Size:        341     511
 
PpuMI rG'GwC_Cy

Cuts at:      0     541     852 
   Size:        541     311
 
PspOMI G'GGCC_C

Cuts at:      0      51     852 
   Size:         51     801
 
PspXI vC'TCGA_Gb

Cuts at:      0     502     852 
   Size:        502     350
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     433     465     852 
   Size:        433      32     387

  Fragments arranged by size: 

                 433     387      32
 
PstI C_TGCA'G

Cuts at:      0      87     722     851     852 
   Size:         87     635     129       1

  Fragments arranged by size: 

                 635     129      87       1
 
PvuII CAG'CTG

Cuts at:      0      88     125     495     852 
   Size:         88      37     370     357

  Fragments arranged by size: 

                 370     357      88      37
 
SacI G_AGCT'C

Cuts at:      0     683     852 
   Size:        683     169
 
SacII CC_GC'GG

Cuts at:      0      10     852 
   Size:         10     842
 
SanDI GG'GwC_CC

Cuts at:      0     541     852 
   Size:        541     311
 
SfcI C'TryA_G

Cuts at:      0      83     718     847     852 
   Size:         83     635     129       5

  Fragments arranged by size: 

                 635     129      83       5
 
SmaI CCC'GGG

Cuts at:      0      56     852 
   Size:         56     796
 
SmlI C'TyrA_G

Cuts at:      0     502     574     852 
   Size:        502      72     278

  Fragments arranged by size: 

                 502     278      72
 
StyI C'CwwG_G

Cuts at:      0     107     852 
   Size:        107     745
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     294     852 
   Size:        294     558
 
XhoI C'TCGA_G

Cuts at:      0     502     852 
   Size:        502     350
 
XmaI C'CCGG_G

Cuts at:      0      54     852 
   Size:         54     798
 

 Enzymes that do cut:

     AccI   AflIII     AhdI    AlwNI     ApaI    ApaLI     AvaI    BamHI
    BanII    BbvCI    BglII Bme1580I     BmrI     BpmI   Bpu10I    BpuEI
    BsaAI    BsaBI    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I    BspHI
    BspMI    BsrBI    BsrFI    BssSI    BstYI     BtgI     BtsI   DraIII
     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I    Hin4I  HindIII     MslI
   MspA1I     PfoI     PmlI    PpuMI   PspOMI    PspXI     PsrI     PstI
    PvuII     SacI    SacII    SanDI     SfcI     SmaI     SmlI     StyI
     XcmI     XhoI     XmaI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII     AgeI
     AleI     AlfI     AloI     ApoI     AscI     AseI    AsiSI    AvrII
     BaeI     BanI     BbeI     BbsI     BcgI    BciVI     BclI    BfrBI
     BglI     BlpI    BmgBI     BmtI     BplI     BsaI    BsaHI    BsaWI
    BsaXI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BsrDI    BsrGI
   BssHII   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI
     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EciI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PmeI     PpiI    PshAI     PsiI
     PvuI    RsrII     SalI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XmnI
     ZraI
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