Restriction map for pF1KE9532
##################################
# NO CUT ENZYMES (  121 enzymes) #
##################################

AccI        AccIII      AclI        AcsI        AflII       
AgeI        AhdI        Alw44I      AlwNI       Aor51HI     
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     AspEI       AspI        AvrII       BamHI       
BbrPI       BbvCI       BcgI        BciVI       BclI        
BfaI        BfrI        BglII       BlnI        BlpI        
BpmI        Bpu1102I    BsaAI       BsaBI       BsaI        
BseAI       BseRI       BsiEI       BsiWI       Bsp1407I    
BspDI       BspEI       BsrBI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstBI       BstZ17I     
CelII       Cfr10I      ClaI        CpoI        DraI        
DraIII      EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoNI       EcoRI       EcoRV       EcoT22I     
FbaI        FseI        HindIII     HpaI        MaeI        
MamI        MfeI        MluI        MroI        MunI        
NaeI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshBI       Psp1406I    PstI        
PvuI        RsrII       SacI        SalI        SapI        
SbfI        ScaI        SexAI       SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SwaI        TfiI        Tsp509I     TspRI       
Tth111I     XbaI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AatII         478  GACGTC               GACGT^C
Acc65I       1095  GGTACC               G^GTACC
AflIII        398  ACATGT               A^C[GA][CT]GT
AluI          934  AGCT                 AG^CT
Asp718I      1095  GGTACC               G^GTACC
AspHI         682  GTGCTC               G[AT]GC[AT]^C
AviII        1046  TGCGCA               TGC^GCA
BbeI          127  GGCGCC               GGCGC^C
BsaWI         764  ACCGGA               [AT]^CCGG[AT]
BsiHKAI       682  GTGCTC               G[AT]GC[AT]^C
BsmBI        1090  GAGACG               CGTCTC(1/5)
BsmI          357  GAATGC               GAATGC(1/-1)
BspHI         710  TCATGA               T^CATGA
BspLU11I      398  ACATGT               A^CATGT
BsrI          629  ACTGG                ACTGG(1/-1)
BstEII        153  GGTCACC              G^GT[ACGT]ACC
BstPI         153  GGTCACC              G^GT[ACGT]ACC
BstYI         608  AGATCC               [GA]^GATC[CT]
DrdI          289  GACACTCTGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I       153  GGTCACC              G^GT[ACGT]ACC
FspI         1046  TGCGCA               TGC^GCA
HaeII         127  GGCGCC               [GA]GCGC^[CT]
HincII        928  GTCAAC               GT[CT]^[GA]AC
HindII        928  GTCAAC               GT[CT]^[GA]AC
HinfI         731  GAGTC                G^A[ACGT]TC
KasI          127  GGCGCC               G^GCGCC
KpnI         1095  GGTACC               GGTAC^C
KspI         1099  CCGCGG               CCGC^GG
MaeIII        154  GTCAC                ^GT[ACGT]AC
MflI          608  AGATCC               [GA]^GATC[CT]
MseI          272  TTAA                 T^TAA
NarI          127  GGCGCC               GG^CGCC
NspI          398  ACATGT               [GA]CATG^[CT]
PflMI         855  CCAAGGGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          731  GAGTC                GAGTC(4/5)
PshAI         828  GACGCCTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII         933  CAGCTG               CAG^CTG
RcaI          710  TCATGA               T^CATGA
SacII        1099  CCGCGG               CCGC^GG
SfcI          702  CTACAG               C^T[GA][CT]AG
SfiI          516  GGCCATCTGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI          127  GGCGCC               GGC^GCC
SmlI          148  CTCAAG               C^T[CT][GA]AG
SspI          259  AATATT               AAT^ATT
StuI          980  AGGCCT               AGG^CCT
Tru9I         272  TTAA                 T^TAA
Tsp45I        154  GTCAC                ^GT[GC]AC
Van91I        855  CCAAGGGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          518  CCATCTGGGCCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         608  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AfaI          219  GTAC                 GT^AC
             1096  GTAC                
AocI          228  CCTCAGG              CC^T[ACGT]AGG
              619  CCTCAGG             
ApaI          524  GGGCCC               GGGCC^C
              633  GGGCCC              
AvaII         297  GGTCC                G^G[AT]CC
              762  GGACC               
BalI          515  TGGCCA               TGG^CCA
              893  TGGCCA              
BanI          127  GGCGCC               G^G[CT][GA]CC
             1095  GGTACC              
BbsI          246  GAAGAC               GAAGAC(2/6)
              841  GTCTTC              
BcnI          636  CCCGG                CC^[GC]GG
             1020  CCGGG               
BglI          138  GCCCCTCGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              517  GCCATCTGGGC         
BpuAI         246  GAAGAC               GAAGAC(2/6)
              841  GTCTTC              
BsgI          909  CTGCAC               GTGCAG(16/14)
             1027  GTGCAG              
BsmAI         883  GAGAC                GTCTC(1/5)
             1090  GAGAC               
BspMI         770  ACCTGC               ACCTGC(4/8)
             1029  GCAGGT              
BsrDI         378  CATTGC               GCAATG(2/0)
             1053  CATTGC              
BstAPI        102  GCATCTGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              992  GCAAGTTCTGC         
BstXI         428  CCATGAGTGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1058  CCAAGGACGTGG        
Bsu36I        228  CCTCAGG              CC^T[ACGT]AGG
              619  CCTCAGG             
DraII         524  GGGCCCT              [GA]G^G[ACGT]CC[CT]
              632  GGGGCCC             
Eco81I        228  CCTCAGG              CC^T[ACGT]AGG
              619  CCTCAGG             
EcoO109I      524  GGGCCCT              [GA]G^G[ACGT]CC[CT]
              632  GGGGCCC             
EcoT14I       855  CCAAGG               C^C[AT][AT]GG
             1058  CCAAGG              
HgaI          306  GACGC                GACGC(5/10)
              828  GACGC               
MluNI         515  TGGCCA               TGG^CCA
              893  TGGCCA              
MscI          515  TGGCCA               TGG^CCA
              893  TGGCCA              
MspA1I        933  CAGCTG               C[AC]G^C[GT]G
             1099  CCGCGG              
NciI          636  CCCGG                CC^[GC]GG
             1020  CCGGG               
RsaI          219  GTAC                 GT^AC
             1096  GTAC                
StyI          855  CCAAGG               C^C[AT][AT]GG
             1058  CCAAGG              


#########################################
# 3 OR MORE CUT ENZYMES (   59 enzymes) #
#########################################

AccII       AciI        AcyI        AlwI        AvaI        
BanII       BbvI        BmyI        BsaHI       BsaJI       
BsiYI       BslI        BsmFI       BsoBI       Bsp1286I    
BssKI       BstF5I      BstNI       BstUI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DsaI        
EaeI        EarI        EcoRII      FauI        Fnu4HI      
FokI        HaeIII      HapII       HhaI        Hin1I       
HinP1I      HpaII       HphI        ItaI        Ksp632I     
MaeII       MboI        MboII       MnlI        MslI        
MspI        MvaI        MvnI        MwoI        NdeII       
NlaIII      NlaIV       Sau3AI      Sau96I      ScrFI       
SfaNI       TaqI        TseI        TthHB8I     
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