Restriction map for pF1KE9532
 (Linear) (Six-base) MAPSORT of: KIEE9532.seq  Check: 261  from: 1  to: 1110



KIEE9532.orf

 With 182 enzymes: SgfI * 

                        December 2, 2013 11:07  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1020    1110 
   Size:       1020      90
 
AatII G_ACGT'C

Cuts at:      0     482    1110 
   Size:        482     628
 
Acc65I G'GTAC_C

Cuts at:      0    1095    1110 
   Size:       1095      15
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      37    1110 
   Size:         37    1073
 
AflIII A'CryG_T

Cuts at:      0     398    1110 
   Size:        398     712
 
AleI CACnn'nnGTG

Cuts at:      0     160    1110 
   Size:        160     950
 
ApaI G_GGCC'C

Cuts at:      0     528     637    1110 
   Size:        528     109     473

  Fragments arranged by size: 

                 528     473     109
 
AvaI C'yCGr_G

Cuts at:      0     142     744     752    1110 
   Size:        142     602       8     358

  Fragments arranged by size: 

                 602     358     142       8
 
BanI G'GyrC_C

Cuts at:      0     127    1095    1110 
   Size:        127     968      15

  Fragments arranged by size: 

                 968     127      15
 
BanII G_rGCy'C

Cuts at:      0       8      79     149     170     197     528     568     637 
   Size:          8      71      70      21      27     331      40      69

Cuts at:    637     751    1110 
   Size:        114     359

  Fragments arranged by size: 

                 359     331     114      71      70      69      40      27
                  21       8
 
BbeI G_GCGC'C

Cuts at:      0     131    1110 
   Size:        131     979
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     253     834    1110 
   Size:        253     581     276

  Fragments arranged by size: 

                 581     276     253
 
BglI GCCn_nnn'nGGC

Cuts at:      0     144     523    1110 
   Size:        144     379     587

  Fragments arranged by size: 

                 587     379     144
 
Bme1580I G_kGCm'C

Cuts at:      0     325     474     528     637    1110 
   Size:        325     149      54     109     473

  Fragments arranged by size: 

                 473     325     149     109      54
 
BmgBI CAC'GTC

Cuts at:      0     488    1026    1065    1110 
   Size:        488     538      39      45

  Fragments arranged by size: 

                 538     488      45      39
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     639    1110 
   Size:        639     471
 
Bpu10I CC'TnA_GC

Cuts at:      0      73    1110 
   Size:         73    1037
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     133    1110 
   Size:        133     977
 
BsaHI Gr'CG_yC

Cuts at:      0     128     479     829    1110 
   Size:        128     351     350     281

  Fragments arranged by size: 

                 351     350     281     128
 
BsaWI w'CCGG_w

Cuts at:      0     764    1110 
   Size:        764     346
 
BseYI C'CCAG_C

Cuts at:      0     861    1110 
   Size:        861     249
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     894    1048    1110 
   Size:        894     154      62

  Fragments arranged by size: 

                 894     154      62
 
BsiHKAI G_wGCw'C

Cuts at:      0     686    1110 
   Size:        686     424
 
BsmI GAATG_Cn'

Cuts at:      0     363    1110 
   Size:        363     747
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1084    1110 
   Size:       1084      26
 
Bsp1286I G_dGCh'C

Cuts at:      0       8      79     149     170     197     325     474     528 
   Size:          8      71      70      21      27     128     149      54

Cuts at:    528     568     637     686     751    1110 
   Size:         40      69      49      65     359

  Fragments arranged by size: 

                 359     149     128      71      70      69      65      54
                  49      40      27      21       8
 
BspHI T'CATG_A

Cuts at:      0     710    1110 
   Size:        710     400
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     779    1020    1110 
   Size:        779     241      90

  Fragments arranged by size: 

                 779     241      90
 
BsrDI GCAATG_nn'

Cuts at:      0     377    1052    1110 
   Size:        377     675      58

  Fragments arranged by size: 

                 675     377      58
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     108     998    1110 
   Size:        108     890     112

  Fragments arranged by size: 

                 890     112     108
 
BstEII G'GTnAC_C

Cuts at:      0     153    1110 
   Size:        153     957
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     435    1065    1110 
   Size:        435     630      45

  Fragments arranged by size: 

                 630     435      45
 
BstYI r'GATC_y

Cuts at:      0     608    1110 
   Size:        608     502
 
Bsu36I CC'TnA_GG

Cuts at:      0     229     620    1110 
   Size:        229     391     490

  Fragments arranged by size: 

                 490     391     229
 
BtgI C'CryG_G

Cuts at:      0     123     726     890    1099    1110 
   Size:        123     603     164     209      11

  Fragments arranged by size: 

                 603     209     164     123      11
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     295    1110 
   Size:        295     815
 
EaeI y'GGCC_r

Cuts at:      0     176     284     346     515     893    1110 
   Size:        176     108      62     169     378     217

  Fragments arranged by size: 

                 378     217     176     169     108      62
 
EarI CTCTTCn'nnn_

Cuts at:      0      16     581     667    1110 
   Size:         16     565      86     443

  Fragments arranged by size: 

                 565     443      86      16
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     724    1110 
   Size:        724     386
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      37    1110 
   Size:         37    1073
 
EcoO109I rG'GnC_Cy

Cuts at:      0     525     633    1110 
   Size:        525     108     477

  Fragments arranged by size: 

                 525     477     108
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1002    1110 
   Size:       1002     108
 
FspI TGC'GCA

Cuts at:      0    1048    1110 
   Size:       1048      62
 
HaeII r_GCGC'y

Cuts at:      0     131    1110 
   Size:        131     979
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     423     455    1110 
   Size:        423      32     655

  Fragments arranged by size: 

                 655     423      32
 
HincII GTy'rAC

Cuts at:      0     930    1110 
   Size:        930     180
 
KasI G'GCGC_C

Cuts at:      0     127    1110 
   Size:        127     983
 
KpnI G_GTAC'C

Cuts at:      0    1099    1110 
   Size:       1099      11
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     168    1110 
   Size:        168     942
 
MscI TGG'CCA

Cuts at:      0     517     895    1110 
   Size:        517     378     215

  Fragments arranged by size: 

                 517     378     215
 
MslI CAynn'nnrTG

Cuts at:      0     160     241     433    1110 
   Size:        160      81     192     677

  Fragments arranged by size: 

                 677     192     160      81
 
MspA1I CmG'CkG

Cuts at:      0     935    1101    1110 
   Size:        935     166       9

  Fragments arranged by size: 

                 935     166       9
 
NarI GG'CG_CC

Cuts at:      0     128    1110 
   Size:        128     982
 
NspI r_CATG'y

Cuts at:      0     402    1110 
   Size:        402     708
 
PasI CC'CwG_GG

Cuts at:      0     918    1110 
   Size:        918     192
 
PciI A'CATG_T

Cuts at:      0     398    1110 
   Size:        398     712
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     861    1110 
   Size:        861     249
 
PfoI T'CCnGG_A

Cuts at:      0     962    1110 
   Size:        962     148
 
PshAI GACnn'nnGTC

Cuts at:      0     832    1110 
   Size:        832     278
 
PspOMI G'GGCC_C

Cuts at:      0     524     633    1110 
   Size:        524     109     477

  Fragments arranged by size: 

                 524     477     109
 
PvuII CAG'CTG

Cuts at:      0     935    1110 
   Size:        935     175
 
SacII CC_GC'GG

Cuts at:      0    1102    1110 
   Size:       1102       8
 
SfcI C'TryA_G

Cuts at:      0     702    1110 
   Size:        702     408
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     523    1110 
   Size:        523     587
 
SfoI GGC'GCC

Cuts at:      0     129    1110 
   Size:        129     981
 
SmlI C'TyrA_G

Cuts at:      0     148    1110 
   Size:        148     962
 
SspI AAT'ATT

Cuts at:      0     261    1110 
   Size:        261     849
 
StuI AGG'CCT

Cuts at:      0     982    1110 
   Size:        982     128
 
StyI C'CwwG_G

Cuts at:      0     855    1058    1110 
   Size:        855     203      52

  Fragments arranged by size: 

                 855     203      52
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     525    1110 
   Size:        525     585
 
ZraI GAC'GTC

Cuts at:      0     480    1110 
   Size:        480     630
 

 Enzymes that do cut:

     AarI    AatII   Acc65I     AcuI   AflIII     AleI     ApaI     AvaI
     BanI    BanII     BbeI     BbsI     BglI Bme1580I    BmgBI     BmrI
   Bpu10I    BpuEI    BsaHI    BsaWI    BseYI     BsgI  BsiHKAI     BsmI
    BsmBI Bsp1286I    BspHI    BspMI    BsrDI   BstAPI   BstEII    BstXI
    BstYI   Bsu36I     BtgI     DrdI     EaeI     EarI     EciI  Eco57MI
 EcoO109I     FalI     FspI    HaeII    Hin4I   HincII     KasI     KpnI
     MmeI     MscI     MslI   MspA1I     NarI     NspI     PasI     PciI
    PflMI     PfoI    PshAI   PspOMI    PvuII    SacII     SfcI     SfiI
     SfoI     SmlI     SspI     StuI     StyI     XcmI     ZraI

 Enzymes that do not cut: 

     AccI     AclI     AfeI    AflII     AgeI     AhdI     AlfI     AloI
    AlwNI    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI
    BamHI    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII     BlpI
     BmtI     BplI     BpmI     BsaI    BsaAI    BsaBI    BsaXI    BseRI
    BsiEI    BsiWI    BspEI    BsrBI    BsrFI    BsrGI   BssHII    BssSI
    BstBI  BstZ17I    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     EagI  EcoICRI    EcoNI    EcoRI    EcoRV     FseI    FspAI  HindIII
     HpaI     MfeI     MluI     NaeI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PmeI     PmlI     PpiI    PpuMI
     PsiI    PspXI     PsrI     PstI     PvuI    RsrII     SacI     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SwaI    TaqII     TatI     TstI
  Tth111I     XbaI     XhoI     XmaI     XmnI
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